同步亚硝化/反硝化除磷的调控因子及菌群分析
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摘要
针对碳源偏低的城市污水,文章采用厌氧/限氧的连续流活性污泥反应器,控制水力停留时间为14 h,污泥回流比为1,COD为80～180 mg/L、TP为8.95～12.25 mg/L、NH_4~+-N为30～33.5 mg/L,考察溶解氧(DO)和二沉池沉淀时间对亚硝化/反硝化同步反应的影响,并对系统微生物菌群进行研究分析。结果表明,污泥中AOB与NDPAOs 2种菌群属类的配比为1.113时,DO范围在0.4～0.7 mg/L,二沉池沉淀时间为3 h,A/OLA连续流中亚硝化和反硝化2个生化反应平衡,脱氮除磷效果最佳,TP的去除率为98.32%,TN的去除率为98.61%。
For the treatment of urban sewage with low carbon source, the anaerobic/limit oxygen continuous flow activated sludge reactor was used under the hydraulic retention time for 14 h, the sludge reflux ratio 1, COD 80～180 mg/L, and TP 8.95～12.25 mg/L, NH_4~+-N 30～33.5 mg/L. The influence of dissolved oxygen(DO) and sedimentation time on the simultaneous nitrosation/denitrification reaction was investigated, and the microbial flora of the system was studied. The results showed that when the ratio of both AOB and NDPAOs in the sludge was 1.113, the DO range was 0.4～0.7 mg/L, the precipitation time in the secondary sedimentation tank was 3 h, the two biochemical reactions of nitrification and denitrification in A/OLA continuous flow were balanced, and the removal efficiency of phosphorus and nitrogen and phosphorus was the best, with average removal rate of TP as 98.32%, and the removal rate of TN as 98.61%.
For the treatment of urban sewage with low carbon source, the anaerobic/limit oxygen continuous flow activated sludge reactor was used under the hydraulic retention time for 14 h, the sludge reflux ratio 1, COD 80～180 mg/L, and TP 8.95～12.25 mg/L, NH_4~+-N 30～33.5 mg/L. The influence of dissolved oxygen(DO) and sedimentation time on the simultaneous nitrosation/denitrification reaction was investigated, and the microbial flora of the system was studied. The results showed that when the ratio of both AOB and NDPAOs in the sludge was 1.113, the DO range was 0.4～0.7 mg/L, the precipitation time in the secondary sedimentation tank was 3 h, the two biochemical reactions of nitrification and denitrification in A/OLA continuous flow were balanced, and the removal efficiency of phosphorus and nitrogen and phosphorus was the best, with average removal rate of TP as 98.32%, and the removal rate of TN as 98.61%.
引文
[1]刘钢.新型后置反硝化工艺处理低C/N(C/P)比城镇污水性能研究[D].杭州:浙江大学,2012.Liu Gang.A New Post-Denitrification Process for the Treatment of Low C/N(C/P)Ratio of Urban Sewage[D].Hangzhou:Zhejiang University,2012.
[2]Li D,Fang Q,Yi D.Maximizing the accumulation of poly-β-hydroxybutyrate(PHB)in low-carbon urban sewage[J].Desalination&Water Treatment,2016:1-12.
[3]易丹,方茜,张可方,等.厌氧时聚-β-羟基丁酸酯(PHB)最大化积累的影响因子[J].环境工程学报,2015,9(1):131-136.Yi Dan,Fang Qian,Zhang Kefang,et al.Effect factors of maximizing accumulation of poly-β-hydroxybutyrate(PHB)during anaerobic[J].Journal of Environmental Engineering,2015,9(1):131-136.
[4]方茜,张朝升,张立秋,等.基于PHB碳源驱动的同时硝化/反硝化除磷过程[J].环境科学学报,2014,34(8):1968-1977.Fang Qian,Zhang Chaosheng,Zhang Liqiu,et al.Simultaneous nitrification/denitrification phosphorus removal process based on PHB carbon source[J].Journal of Environmental Engineering,2014,34(8):1968-1977.
[5]张朝升,范建华,张可方,等.亚硝酸盐型同步硝化反硝化耦合除磷研究[J].环境科学与技术,2007,30(8):13-15.Zhang Chaosheng,Fan Jianhua,Zhang Kefang,et al.Study on nitrite-type simultaneous nitrification and denitrification coupled phosphorus removal[J].Environmental Science and Technology,2007,30(8):13-15.
[6]徐诗燕,方茜,易丹,等.亚硝化/反硝化除磷同步发生的控制策略[J].环境工程学报,2017,11(3):1487-1493.Xu Shiyan,Fang Qian,Yi Dan,et al.Control strategy for simultaneous occurrence of nitrosation/denitrifying phosphorus removal[J].Journal of Environmental Engineering,2017,11(3):1487-1493.
[7]张立秋,张可方,张朝升,等.DO对亚硝酸型SND的影响[J].水处理技术,2008(8):29-33.Zhang Liqiu,Zhang Kefang,Zhang Chaosheng,et al.Effect of DO on nitrite-type SND[J].Water Treatment Technology,2008(8):29-33.
[8]荣宏伟,张朝升,彭永臻,等.DO对SBBR工艺同步硝化反硝化的影响研究[J].环境科学与技术,2009,32(8):16-19.Rong Hongwei,Zhang Chaosheng,Peng Yongzhen,et al.Effect of DO on simultaneous nitrification and denitrification in SBBR process[J].Environmental Science and Technology,2009,32(8):16-19.
[9]罗思音.DO对短程同步硝化反硝化除磷工艺的影响[J].水科学与工程技术,2011(5):18-20.Luo Yinsi.Effect of DO on short-range simultaneous nitrification and denitrifying phosphorus removal process[J].Water Science and Engineering Technology,2011(5):18-20.
[10]张华,张学洪,郭周芳,等.微孔曝气氧化沟生物脱氮除磷影响因素的研究[J].水处理技术,2014(4):103-106.Zhang Hua,Zhang Xuehong,Guo Zhoufang,et al.Study on the factors affecting biological nitrogen and phosphorus removal in microporous aeration oxidation ditch[J].Water Treatment Technology,2014(4):103-106.
[11]贾西宁,汤婷.二沉池浮泥产生的影响因素及避免措施[C].//全国污泥处理技术研究暨资源化综合利用新技术、新设备交流研讨会.2013.Jia Xining,Tang Ting.Influencing Factors and Avoidance Measures of Floating Mud in the Secondary Sedimentation Tank[C].//National Seminar on Sludge Treatment Technology Research and Resource Utilization Comprehensive Utilization of New Technologies and New Equipment,2013.
[12]Wang J,Yu J.Kinetic analysis on formation of poly(3-hydroxybutyrate)from acetic acid by Ralstonia eutropha,under chemically defined conditions[J].Journal of Industrial Microbiology&Biotechnology,2001,26(3):121.
[13]Wang X,Wen X,Yan H,et al.Bacterial community dynamics in a functionally stable pilot-scale wastewater treatment plant[J].Bioresource Technology,2011,102(3):2352-7.
[14]Zhang T,Shao M F,Ye L.454 pyrosequencing reveals bacterial diversity of activated sludge from 14 sewage treatment plants[J].ISME Journal,2012,6(6):1137-1147.
[15]郑林雪,李军,胡家玮,等.同步硝化反硝化系统中反硝化细菌多样性研究[J].中国环境科学,2015(1):116-121.Zheng Linxue,Li Jun,Hu Jiawei,et al.Study on the diversity of denitrifying bacteria in simultaneous nitrification and denitrification systems[J].China Environmental Science,2015(1):116-121.
[16]Allen M S,Welch K T,Prebyl B S,et al.Analysis and glycosyl composition of the exopolysaccharide isolated from the floc-forming wastewater bacterium Thauera sp.MZ1T[J].Environmental Microbiology,2004,6(8):780.
[2]Li D,Fang Q,Yi D.Maximizing the accumulation of poly-β-hydroxybutyrate(PHB)in low-carbon urban sewage[J].Desalination&Water Treatment,2016:1-12.
[3]易丹,方茜,张可方,等.厌氧时聚-β-羟基丁酸酯(PHB)最大化积累的影响因子[J].环境工程学报,2015,9(1):131-136.Yi Dan,Fang Qian,Zhang Kefang,et al.Effect factors of maximizing accumulation of poly-β-hydroxybutyrate(PHB)during anaerobic[J].Journal of Environmental Engineering,2015,9(1):131-136.
[4]方茜,张朝升,张立秋,等.基于PHB碳源驱动的同时硝化/反硝化除磷过程[J].环境科学学报,2014,34(8):1968-1977.Fang Qian,Zhang Chaosheng,Zhang Liqiu,et al.Simultaneous nitrification/denitrification phosphorus removal process based on PHB carbon source[J].Journal of Environmental Engineering,2014,34(8):1968-1977.
[5]张朝升,范建华,张可方,等.亚硝酸盐型同步硝化反硝化耦合除磷研究[J].环境科学与技术,2007,30(8):13-15.Zhang Chaosheng,Fan Jianhua,Zhang Kefang,et al.Study on nitrite-type simultaneous nitrification and denitrification coupled phosphorus removal[J].Environmental Science and Technology,2007,30(8):13-15.
[6]徐诗燕,方茜,易丹,等.亚硝化/反硝化除磷同步发生的控制策略[J].环境工程学报,2017,11(3):1487-1493.Xu Shiyan,Fang Qian,Yi Dan,et al.Control strategy for simultaneous occurrence of nitrosation/denitrifying phosphorus removal[J].Journal of Environmental Engineering,2017,11(3):1487-1493.
[7]张立秋,张可方,张朝升,等.DO对亚硝酸型SND的影响[J].水处理技术,2008(8):29-33.Zhang Liqiu,Zhang Kefang,Zhang Chaosheng,et al.Effect of DO on nitrite-type SND[J].Water Treatment Technology,2008(8):29-33.
[8]荣宏伟,张朝升,彭永臻,等.DO对SBBR工艺同步硝化反硝化的影响研究[J].环境科学与技术,2009,32(8):16-19.Rong Hongwei,Zhang Chaosheng,Peng Yongzhen,et al.Effect of DO on simultaneous nitrification and denitrification in SBBR process[J].Environmental Science and Technology,2009,32(8):16-19.
[9]罗思音.DO对短程同步硝化反硝化除磷工艺的影响[J].水科学与工程技术,2011(5):18-20.Luo Yinsi.Effect of DO on short-range simultaneous nitrification and denitrifying phosphorus removal process[J].Water Science and Engineering Technology,2011(5):18-20.
[10]张华,张学洪,郭周芳,等.微孔曝气氧化沟生物脱氮除磷影响因素的研究[J].水处理技术,2014(4):103-106.Zhang Hua,Zhang Xuehong,Guo Zhoufang,et al.Study on the factors affecting biological nitrogen and phosphorus removal in microporous aeration oxidation ditch[J].Water Treatment Technology,2014(4):103-106.
[11]贾西宁,汤婷.二沉池浮泥产生的影响因素及避免措施[C].//全国污泥处理技术研究暨资源化综合利用新技术、新设备交流研讨会.2013.Jia Xining,Tang Ting.Influencing Factors and Avoidance Measures of Floating Mud in the Secondary Sedimentation Tank[C].//National Seminar on Sludge Treatment Technology Research and Resource Utilization Comprehensive Utilization of New Technologies and New Equipment,2013.
[12]Wang J,Yu J.Kinetic analysis on formation of poly(3-hydroxybutyrate)from acetic acid by Ralstonia eutropha,under chemically defined conditions[J].Journal of Industrial Microbiology&Biotechnology,2001,26(3):121.
[13]Wang X,Wen X,Yan H,et al.Bacterial community dynamics in a functionally stable pilot-scale wastewater treatment plant[J].Bioresource Technology,2011,102(3):2352-7.
[14]Zhang T,Shao M F,Ye L.454 pyrosequencing reveals bacterial diversity of activated sludge from 14 sewage treatment plants[J].ISME Journal,2012,6(6):1137-1147.
[15]郑林雪,李军,胡家玮,等.同步硝化反硝化系统中反硝化细菌多样性研究[J].中国环境科学,2015(1):116-121.Zheng Linxue,Li Jun,Hu Jiawei,et al.Study on the diversity of denitrifying bacteria in simultaneous nitrification and denitrification systems[J].China Environmental Science,2015(1):116-121.
[16]Allen M S,Welch K T,Prebyl B S,et al.Analysis and glycosyl composition of the exopolysaccharide isolated from the floc-forming wastewater bacterium Thauera sp.MZ1T[J].Environmental Microbiology,2004,6(8):780.