高效液相色谱指纹图谱法测定广西不同产地粉葛和葛根中9种特征成分
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摘要
目的建立高效液相色谱指纹图谱法测定分析广西不同产地粉葛和葛根中9种特征成分的方法。方法利用高效液相色谱法(high performance liquid chromatography, HPLC)同时测定粉葛和葛根中葛根素、3’-羟基葛根素、大豆苷、黄豆黄苷、染料木苷、大豆苷元、染料木素、刺芒柄花素、鹰嘴豆芽素A等9种特征成分的含量,建立广西不同产地粉葛和葛根的指纹图谱,并利用相似度分析从整体上对广西粉葛和葛根药材的质量进行评价。结果测定了8批次样品中的9种特征成分的含量。粉葛样品共标定15个共有峰,指认其中7个峰,与共有模式之间的相似度均大于0.97;葛根样品共标定34个共有峰,指认其中7个峰,与共有模式之间的相似度均大于0.99。结论广西不同产地的粉葛和葛根之间所含的化学成分具有一定的共性和差异性,该方法适合用于葛根和粉葛的质量控制。
Objective To establish a method for determination and analysis of 9 characteristic constituents in Puerariae thomsonii radix and Puerariae lobatae radix from different producing areas of Guangxi by high performance liquid chromatography fingerprint. Methods The 9 characteristic constituents content of puerarin,3'-hydroxy puerarin, daidzin, glycitin, genistin, daidzein, genistein, formononetin, biochanin A in Puerariae thomsonii radix and Puerariae lobatae radix was determined by high performance liquid chromatography(HPLC).The fingerprints of Puerariae thomsonii radix and Puerariae lobatae radix in different producing areas in Guangxi were established, and the quality of Guangxi Puerariae thomsonii radix and Puerariae lobatae radix were evaluated by similarity analysis. Results The content of 9 characteristic components in 8 batches of samples was determined.In total, 15 common peaks were calibrated by Puerariae thomsonii radix, and 7 of them were identified, the similarity between them and common patterns was greater than 0.97. Thirty-four common peaks were calibrated by Puerariae lobatae radix, and 7 of them were identified, the similarity between them and common patterns was greater than 0.99.Conclusion The chemical composition of Puerariae thomsonii radix and Puerariae lobatae radix from different producing areas in Guangxi has certain commonness and difference. This method is suitable for quality control of of puerariae thomsonii radix and puerariae lobatae radix.
Objective To establish a method for determination and analysis of 9 characteristic constituents in Puerariae thomsonii radix and Puerariae lobatae radix from different producing areas of Guangxi by high performance liquid chromatography fingerprint. Methods The 9 characteristic constituents content of puerarin,3'-hydroxy puerarin, daidzin, glycitin, genistin, daidzein, genistein, formononetin, biochanin A in Puerariae thomsonii radix and Puerariae lobatae radix was determined by high performance liquid chromatography(HPLC).The fingerprints of Puerariae thomsonii radix and Puerariae lobatae radix in different producing areas in Guangxi were established, and the quality of Guangxi Puerariae thomsonii radix and Puerariae lobatae radix were evaluated by similarity analysis. Results The content of 9 characteristic components in 8 batches of samples was determined.In total, 15 common peaks were calibrated by Puerariae thomsonii radix, and 7 of them were identified, the similarity between them and common patterns was greater than 0.97. Thirty-four common peaks were calibrated by Puerariae lobatae radix, and 7 of them were identified, the similarity between them and common patterns was greater than 0.99.Conclusion The chemical composition of Puerariae thomsonii radix and Puerariae lobatae radix from different producing areas in Guangxi has certain commonness and difference. This method is suitable for quality control of of puerariae thomsonii radix and puerariae lobatae radix.
引文
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[8]陈风春,张亦红,刘芳,等.高效液相色谱法测定葛根类保健食品中葛根素、大豆苷、大豆苷元及染料木素[J].解放军预防医学杂志,2013,31(5):425-427.Chen FC,Zhang YH,Liu F,et al.Determination of puerarin,daidzein,daidzein and dyelignin in pueraria health food used by high performance liquid chromatography[J].J Prev Med Chin PLA,2013,31(5):425-427.
[9]吴文杰,邓阳,谭桂林,等.一测多评法测定葛根药材中5种异黄酮类份[J].中草药,2017,48(4):777-781.Wu WJ,Deng Y,Tan GL,et al.Determination of five isoflavonoids in Pueraria Radix by QAMS[J].Chin Trad Herb Drug,2017,48(4):777-781.
[10]尤春雪,张振秋,李峰,等.HPLC波长切换技术对葛根中8种成分的测定及指纹图谱研究[J].中草药,2013,44(5):616-621.You CX,Zhang ZQ,Li F,et al.Determination and fingerprint analysis of eight constituents in puerariae radix by HPLC wavelength switching[J].Chin Trad Herb Drug,2013,44(5):616-621.
[11]刘喜舞,戴玲,叶惠煊,等.HPLC法同时测定中国、泰国葛根中异黄酮的含量[J].中国药师,2013,16(5):668-670.Liu XW,Dai L,Ye HX,et al.Simultaneous determination of isoflavonoids in Radix puerariae from China and Thailand by HPLC[J].China Pharm,2013,16(5):668-670.
[12]王金华,姚仲青.葛根与粉葛饮片的色谱指纹图谱研究[J].医学导报,2013,4(32):525-529.Wang JH,Yao ZQ.Studies on chromatographic fingerprints of Pueraria lobata and Pueraria thomsonii[J].Herald Med,2013,4(32):525-529.
[13]聂平,肖炳燚,罗晖明,等.超高效液相色谱-质谱联用技术在中药指纹图谱中的应用[J].中南药学,2013,11(7):524-527.Nie P,Xiao BY,Luo HM,et al.Application of ultrahigh performance liquid chromatography-mass spectrometry in fingerprint of traditional Chinese medicine[J].Centr South Pharm,2013,11(7):524-527.
[14]戴玲,叶惠煊,邹亲朋,等.湘葛一号根HPLC指纹图谱及5种异黄酮含量的同时测定[J].西北药学杂志,2016,31(3):239-244.Dai L,Ye HX,Zou QP,et al.HPLC fingerprint for roots of Hunan Pueraria thomsonii Benth and simultaneous determination of five isoflavones[J].Northwest Pharm J,2016,31(3):239-244.
[15]李生茂,刘琳,谭睿,等.葛根和粉葛高效液相指纹图谱及化学模式识别[J].辽宁中医药杂志,2016,43(5):1034-1037.Li SM,Liu L,Tan R,et al.HPLC fingerprint and chemical pattern recognition of Pueraria lobata and Pueraria thomsonii[J].Liaoning JTrad Chin Med,2016,43(5):1034-1037.
[2]杨立芳,李小燕,罗伟强,等.高效液相色谱法测定广西粉葛中葛根素的含量[J].时珍国医国药,2007,18(3):578-579.Yang LF,Li XY,Luo WQ,et al.Determination of Puerarinin content in Guangxi Puerariae thomonii radix by HPLC[J].Lishizhen Med Mater Med Res,2007,18(3):578-579.
[3]王黎,高苏亚,李华.不同产地葛根的HPLC指纹图谱及质量评价研究[J].安徽农业科学,2011,39(18):10782.Wang L,Gao SY,Li H.HPLC fingerprints and quality evaluation of Radix puerariae from different places[J].J Anhui Agric Sci,2011,39(18):10782.
[4]何建军,梅新,陈学玲,等.我国葛根产业的开发思路[J].农产品加工学刊,2011,(12):104-105.He JJ,Mei X,Chen XL,et al.Thinking on industry development of Pueraria lobata[J].Acad Period Farm Prod Process,2011,(12):104-105.
[5]高靖,陈继红.葛根及葛根异黄酮的研究现状[J].畜牧与饲料科学,2013,34(6):44-45.Gao J,Chen JH.The current research status of Radix puerariae and Radix puerariae isoflavone[J].Anim Husband Feed Sci,2013,34(6):44-45.
[6]沈梦洁,冯昕韡,朱仲良.葛根HPLC指纹图谱及模式识别研究[J].内蒙古大学学报(自然科学版),2012,43(1):73-76.Shen MJ,Feng XW,Zhu ZL.Study on HPLC fingerprint and pattern recognition of pueraria[J].J Inner Mongolia Univ(Nat Sci Ed),2012,43(1):73-76.
[7]张建辉,杨代明,吴延瑞,等.HPLC法和紫外分光光度法测定葛根淀粉中葛根素含量的研究[J].食品与机械,2006,22(6):110-112.Zhang JH,Yang DM,Wu YR,et al.Comparative studies on puerarin determ ination of kudzu roots starch by ultraviolet spectrophotometry and HPLC[J].Food Mach,2006,22(6):110-112.
[8]陈风春,张亦红,刘芳,等.高效液相色谱法测定葛根类保健食品中葛根素、大豆苷、大豆苷元及染料木素[J].解放军预防医学杂志,2013,31(5):425-427.Chen FC,Zhang YH,Liu F,et al.Determination of puerarin,daidzein,daidzein and dyelignin in pueraria health food used by high performance liquid chromatography[J].J Prev Med Chin PLA,2013,31(5):425-427.
[9]吴文杰,邓阳,谭桂林,等.一测多评法测定葛根药材中5种异黄酮类份[J].中草药,2017,48(4):777-781.Wu WJ,Deng Y,Tan GL,et al.Determination of five isoflavonoids in Pueraria Radix by QAMS[J].Chin Trad Herb Drug,2017,48(4):777-781.
[10]尤春雪,张振秋,李峰,等.HPLC波长切换技术对葛根中8种成分的测定及指纹图谱研究[J].中草药,2013,44(5):616-621.You CX,Zhang ZQ,Li F,et al.Determination and fingerprint analysis of eight constituents in puerariae radix by HPLC wavelength switching[J].Chin Trad Herb Drug,2013,44(5):616-621.
[11]刘喜舞,戴玲,叶惠煊,等.HPLC法同时测定中国、泰国葛根中异黄酮的含量[J].中国药师,2013,16(5):668-670.Liu XW,Dai L,Ye HX,et al.Simultaneous determination of isoflavonoids in Radix puerariae from China and Thailand by HPLC[J].China Pharm,2013,16(5):668-670.
[12]王金华,姚仲青.葛根与粉葛饮片的色谱指纹图谱研究[J].医学导报,2013,4(32):525-529.Wang JH,Yao ZQ.Studies on chromatographic fingerprints of Pueraria lobata and Pueraria thomsonii[J].Herald Med,2013,4(32):525-529.
[13]聂平,肖炳燚,罗晖明,等.超高效液相色谱-质谱联用技术在中药指纹图谱中的应用[J].中南药学,2013,11(7):524-527.Nie P,Xiao BY,Luo HM,et al.Application of ultrahigh performance liquid chromatography-mass spectrometry in fingerprint of traditional Chinese medicine[J].Centr South Pharm,2013,11(7):524-527.
[14]戴玲,叶惠煊,邹亲朋,等.湘葛一号根HPLC指纹图谱及5种异黄酮含量的同时测定[J].西北药学杂志,2016,31(3):239-244.Dai L,Ye HX,Zou QP,et al.HPLC fingerprint for roots of Hunan Pueraria thomsonii Benth and simultaneous determination of five isoflavones[J].Northwest Pharm J,2016,31(3):239-244.
[15]李生茂,刘琳,谭睿,等.葛根和粉葛高效液相指纹图谱及化学模式识别[J].辽宁中医药杂志,2016,43(5):1034-1037.Li SM,Liu L,Tan R,et al.HPLC fingerprint and chemical pattern recognition of Pueraria lobata and Pueraria thomsonii[J].Liaoning JTrad Chin Med,2016,43(5):1034-1037.
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