龙血竭胶囊对SD大鼠放射性皮肤损伤防护作用及机制的初步研究
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摘要
目的:探讨龙血竭对SD大鼠皮肤放射性损伤的防护作用及可能机制。方法:100只雄性SD大鼠,随机数字表法分为5个组:空白对照组、龙血竭对照组、照射模型组、比亚芬治疗组和龙血竭治疗组,每组20只,比亚芬和龙血竭于电子线照射后外用于背部皮肤,观察照射后3天大鼠皮肤组织病理改变,血清和皮肤组织丙二醛(MDA)含量,超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、Na~+-K~+-ATP酶和Ca~(2+)-Mg~(2+)-ATP酶活性,Western Blot方法检测皮肤组织Bcl-2、Bax、Caspase-3蛋白表达水平。结果:龙血竭胶囊可以降低皮肤和血清MDA的含量,增加GSH-Px和SOD的活性,增加皮肤Bcl-2蛋白水平,降低Bax和活化的Caspase-3蛋白水平,对ATP酶活性无影响。结论:龙血竭胶囊对大鼠放射性皮肤损伤有防护作用,其机制可能为提高机体的抗氧化酶和抑制细胞凋亡。
Objective:To explore the possible mechanism of the prevention of Longxuejie capsules in radiomermatitis using a SD rat model.Methods:One-hundred male SD rats were randomly divided into five groups:Blank control group,Longxuejie group,radiation group,biafine+radiation group and Longxuejie+radiation group.Longxuejie or Biafine was applied to the back ski after electron beam irradiation.At 3d after treatment,skin tissue and serum were collected to observe pathological changes,test activities SOD,GSH-Px,Na~+-K~+-ATPase and Ca~(2+)-Mg~(2+)-ATPase as well as the content of MDA,and detect protein expression of Bcl-2,Bax and caspase 3.Results:Longxuejie capsules could reduce the content of MDA in tissue,increased the activities of GSH-Px and SOD,enhanced the expression of Bcl-2 protein level,but reduced the levels of Bax and cleaved Caspase-3.It has no effect on ATPase.Conclusions:Longxuejie capsules could protect rat skin from radiation injury probably by promotion the antioxidant enzymes and inhibition apoptosis.
Objective:To explore the possible mechanism of the prevention of Longxuejie capsules in radiomermatitis using a SD rat model.Methods:One-hundred male SD rats were randomly divided into five groups:Blank control group,Longxuejie group,radiation group,biafine+radiation group and Longxuejie+radiation group.Longxuejie or Biafine was applied to the back ski after electron beam irradiation.At 3d after treatment,skin tissue and serum were collected to observe pathological changes,test activities SOD,GSH-Px,Na~+-K~+-ATPase and Ca~(2+)-Mg~(2+)-ATPase as well as the content of MDA,and detect protein expression of Bcl-2,Bax and caspase 3.Results:Longxuejie capsules could reduce the content of MDA in tissue,increased the activities of GSH-Px and SOD,enhanced the expression of Bcl-2 protein level,but reduced the levels of Bax and cleaved Caspase-3.It has no effect on ATPase.Conclusions:Longxuejie capsules could protect rat skin from radiation injury probably by promotion the antioxidant enzymes and inhibition apoptosis.
引文
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[15]曲静,武霞,刘学鍵.龙血竭治疗头颈部肿瘤放疗后放射性皮肤损伤的疗效观察[J].山东医学高等专科学校学报,2016,38(5):378-381.
[16]朱振风,高学美,武霞.湿润烧伤膏联合龙血竭治疗头颈部肿瘤放射性皮肤损伤的应用研究[J].中国烧伤创疡杂志,2017,29(3):225-228.
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[19]Shepherd BR,Enis DR,Wang F,et al.Vascularization and engraftment of a human skin substitute using circulating progenitor cell-derived endothel ial cells[J].FASEB J,2006,20(10):1739-1741.
[20]张宪发,吴正球,梁自乾,等.糖尿病模型大鼠烫伤创面皮肤组织P物质和Bcl-2表达与龙血竭的干预[J].中国组织工程研究,2012,16(20):3675-3679.
[2]倪晨,王贵均,赵爱国.β射线致皮肤放射性损伤动物模型的建立[J].中药新药与临床药理,2011,22(2):224-225.
[3]Jaschke W,Schmuth M,Trianni A,et al.Radiation-Induced Skin Injuries to Patients:What the Interventional Radiologist Needs to Know[J].Cardiovasc Intervent Radiol,2017,40(8):1131-1140.
[4]Sheng X,Zhou Y,Wang H,et al.Establishment and characterization of a radiation-induced dermatitis rat model[J].J Cell Mol Med,2019,23(5):3178-3189.
[5]Winquist E,Agbassi C,Meyers BM,et al.Systemic therapy in the curative treatment of head and neck squamous cell cancer:a systematic review[J].J Otolaryngol Head Neck Surg,2017,46(1):29.
[6]Yang B,Wang Z,Sheng C,et al.Evidence-based review of oral traditional Chinese medicine compound recipe administration for treating weight drop-induced experimental traumatic brain injury[J].BMC Complement Altern Med,2016,16:95.
[7]师梅梅,杨建雄.龙血竭的体外抗氧化研究[J].中成药,2009,29(11):1591-1594.
[8]Ran Y,Wang R,Lin F,et al.Radioprotective effects of Dragon's blood and its extract against gamma irradiation in mouse bone marrow cells[J].Phys Med,2014,30(4):427-431.
[9]王丽新.黄芪对自然衰老小鼠皮肤中ATP酶活力影响的实验研究[J].实用中医内科杂志,2010,24(9):24-26.
[10]Karbownik M,Reiter RJ.Antioxidative effects of melatonin in protection against cellular damage caused by ionizing radiation[J].Proc Soc Exp Biol Med,2000,225(1):9-22.
[11]Ran Y,Wang R,Gao Q,et al.Dragon's blood and its extracts attenuate radiation-induced oxidative stress in mice[J].J Radiat Res,2014,55(4):699-706.
[12]武霞,盖龙娴,刘学键.龙血竭联合獾油治疗头颈部肿瘤放疗引起放射性皮肤损伤的疗效观察[J].中国基层医药,2015,22(11):1719-1720.
[13]李彦华,武霞.龙血竭联合康复新液治疗宫颈癌同步放化疗引起皮肤损伤的临床研究[J].山东医学高等专科学校学报,2018,40(6):404-406.
[14]许明君,杨静.龙血竭胶囊治疗放射性皮肤损伤的临床疗效观察[J].赣南医学院学报,2011,31(6):739-740.
[15]曲静,武霞,刘学鍵.龙血竭治疗头颈部肿瘤放疗后放射性皮肤损伤的疗效观察[J].山东医学高等专科学校学报,2016,38(5):378-381.
[16]朱振风,高学美,武霞.湿润烧伤膏联合龙血竭治疗头颈部肿瘤放射性皮肤损伤的应用研究[J].中国烧伤创疡杂志,2017,29(3):225-228.
[17]张静,刘丽波.放射性皮肤损伤的机制研究进展[J].辐射研究与辐射工艺学报,2015,33(2):1-6.
[18]Xin N,Li YJ,Li X,et al.Dragon's blood may have radioprotective effects in radiation-induced rat brain injury[J].Radiat Res,2012,178(1):75-85.
[19]Shepherd BR,Enis DR,Wang F,et al.Vascularization and engraftment of a human skin substitute using circulating progenitor cell-derived endothel ial cells[J].FASEB J,2006,20(10):1739-1741.
[20]张宪发,吴正球,梁自乾,等.糖尿病模型大鼠烫伤创面皮肤组织P物质和Bcl-2表达与龙血竭的干预[J].中国组织工程研究,2012,16(20):3675-3679.