GC法同时测定香砂枳术丸中3种成分的含量
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摘要
目的建立同时测定香砂枳术丸中3种成分含量的气相色谱方法。方法色谱柱为HP-5柱(30m×0.32 mm,0.25μm);采取程序升温,载气为氮气,流速为2.0 mL·min~(-1),进样量为1μL,分流比为5∶1,进样口温度为275℃,检测器(FID)温度为295℃。结果乙酸龙脑酯、木香烃内酯、去氢木香内酯分别在12.75～204.0μg·m L-1(r=0.9999)、34.88～558.0μg·mL~(-1)(r=0.9999)、48.68～778.8μg·mL~(-1)(r=0.9999)内线性关系良好,平均加样回收率分别为97.7%(RSD=1.4%)、98.2%(RSD=1.2%)、101.0%(RSD=1.1%)。结论该方法操作简便、准确,可靠,可用于香砂枳术丸的质量控制。
Objective To establish a GC method to simultaneously determine 3 ingredients in Xiangsha Zhizhu pills. Methods The chromatogram column was HP-5(30 m×0.32 mm, 0.25 μm). The programmed-temperature used nitrogen as the medium at 2.0 mL·min~(-1), the injection volume was 1 μL and the split ratio was 5 ∶ 1.The inlet temperature was 275 ℃ and the FID temperature was 295 ℃. Results Good linear correlations were found at 12.75-204.0 μg·mL~(-1)(r = 0.9999) for bornyl acetate, 34.88-558.0 μg·mL~(-1)(r = 0.9999)for costunolide, and 48.68-778.8 μg·mL~(-1)(r = 0.9999) for dehydrocostus lactone. The average recovery(n = 6) were 97.7%(RSD = 1.4%) for bornyl acetate, 98.2%(RSD = 1.2%) for costunolide, and 101.0%(RSD= 1.1%) for dehydrocostus lactone. Conclusion The method is easy, accurate and reliable. It can be used for the quality control of Xiangsha Zhizhu pills.
Objective To establish a GC method to simultaneously determine 3 ingredients in Xiangsha Zhizhu pills. Methods The chromatogram column was HP-5(30 m×0.32 mm, 0.25 μm). The programmed-temperature used nitrogen as the medium at 2.0 mL·min~(-1), the injection volume was 1 μL and the split ratio was 5 ∶ 1.The inlet temperature was 275 ℃ and the FID temperature was 295 ℃. Results Good linear correlations were found at 12.75-204.0 μg·mL~(-1)(r = 0.9999) for bornyl acetate, 34.88-558.0 μg·mL~(-1)(r = 0.9999)for costunolide, and 48.68-778.8 μg·mL~(-1)(r = 0.9999) for dehydrocostus lactone. The average recovery(n = 6) were 97.7%(RSD = 1.4%) for bornyl acetate, 98.2%(RSD = 1.2%) for costunolide, and 101.0%(RSD= 1.1%) for dehydrocostus lactone. Conclusion The method is easy, accurate and reliable. It can be used for the quality control of Xiangsha Zhizhu pills.
引文
[1]中国药典2015年版.一部[S]. 2015:1203-1204.
[2]邢亚红,王芬,钱胜华. GC-MS测定香砂平胃丸中乙酸龙脑酯的含量[J].海峡药学,2015,27(9):43-45.
[3]马丽娜,郭伟英.毛细管气相色谱法同时测定沉香化气丸中3个成分的含量[J].药物分析杂志,2012,32(9):1559-1563.
[4]袁惠霞,欧国灯,刘潇潇.气相色谱法测定香砂六君丸中乙酸龙脑酯[J].中国医药研究,2018,8(19):69-71.
[5]赵磊,郭彩芳,高楠楠,等. GC法同时测定香砂养胃片中、厚朴酚、和厚朴酚的含量[J].中国药物评价,2018,35(5):339-342.
[6]张小龙,孙晓,姚晨旭,等. GC法同时测定肚痛丸中6种有效成分的含量[J].中国药师,2018,21(3):518-520.
[7]鲁艺,申丽,王洋,等.砂仁挥发油中7种活性成分的含量测定研究[J].药物分析杂志,2016,36(9):1536-1543.
[8]赵磊,郭彩芳,高楠楠,等. GC法同时测定香砂养胃片中去氢木香内酯、厚朴酚、和厚朴酚的含量[J].中国药物评价,2018,35(5):339-342.
[9]陈正收,周应军,胡达,等.高效液相色谱法测定调经活血片中木香烃内酯和去氢木香内酯的含量[J].中南药学,2007,5(6):524-526.
[2]邢亚红,王芬,钱胜华. GC-MS测定香砂平胃丸中乙酸龙脑酯的含量[J].海峡药学,2015,27(9):43-45.
[3]马丽娜,郭伟英.毛细管气相色谱法同时测定沉香化气丸中3个成分的含量[J].药物分析杂志,2012,32(9):1559-1563.
[4]袁惠霞,欧国灯,刘潇潇.气相色谱法测定香砂六君丸中乙酸龙脑酯[J].中国医药研究,2018,8(19):69-71.
[5]赵磊,郭彩芳,高楠楠,等. GC法同时测定香砂养胃片中、厚朴酚、和厚朴酚的含量[J].中国药物评价,2018,35(5):339-342.
[6]张小龙,孙晓,姚晨旭,等. GC法同时测定肚痛丸中6种有效成分的含量[J].中国药师,2018,21(3):518-520.
[7]鲁艺,申丽,王洋,等.砂仁挥发油中7种活性成分的含量测定研究[J].药物分析杂志,2016,36(9):1536-1543.
[8]赵磊,郭彩芳,高楠楠,等. GC法同时测定香砂养胃片中去氢木香内酯、厚朴酚、和厚朴酚的含量[J].中国药物评价,2018,35(5):339-342.
[9]陈正收,周应军,胡达,等.高效液相色谱法测定调经活血片中木香烃内酯和去氢木香内酯的含量[J].中南药学,2007,5(6):524-526.