脂质组学分析方法进展及其在中药研究中的应用
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摘要
脂质在生物学过程中发挥着广泛而重要的作用。作为代谢组学的分支之一,脂质组学通过探究不同生理、病理状态或药物干预后整体脂质代谢的变化,从脂质代谢网络中寻找疾病的关键脂质标志物,从整体角度研究脂质代谢调控在疾病发生、发展中的作用机制及药物作用机制。由于中药成分的复杂性和作用的整体性,亟需发展适合中药复杂体系研究的科学方法揭示其药效物质、整体作用机制等科学内涵。基于脂质组学的系统生物学技术为中药复杂体系作用机制研究提供了可借鉴的思路。该文主要就脂质组学方法技术及其在中药研究中的应用进行综述,以期为中药现代化研究提供方法技术参考。
Lipids have been documented to play comprehensive and significant role in many biological processes. As a branch of metabolomics,lipidomics research mainly involves the analysis of the variation of lipid metabolism profiles under different physiologic,pathologic conditions or drug intervention,the discovery of key lipid biomarkers of a disease in lipid metabolic networks,and the study of the mechanism of action of lipid metabolic regulation during disease onset and progression,and drug treatment. Traditional Chinese medicines( TCMs)are characterized with integrated effects by multi-components,multi-targets and integrated effects. It is urgent to develop methods suitable for the study of complex TCMs to reveal the active constituents and integrated mechanism of action. Systems biology such as lipidomics provides valuable strategy and approach to illustrate the complex mechanisms of TCMs. In this paper,in order to provide technical references for TCMs,we have reviewed the analytical techniques applied in lipidomics and the applications of lipidomics in TCMs researches.
Lipids have been documented to play comprehensive and significant role in many biological processes. As a branch of metabolomics,lipidomics research mainly involves the analysis of the variation of lipid metabolism profiles under different physiologic,pathologic conditions or drug intervention,the discovery of key lipid biomarkers of a disease in lipid metabolic networks,and the study of the mechanism of action of lipid metabolic regulation during disease onset and progression,and drug treatment. Traditional Chinese medicines( TCMs)are characterized with integrated effects by multi-components,multi-targets and integrated effects. It is urgent to develop methods suitable for the study of complex TCMs to reveal the active constituents and integrated mechanism of action. Systems biology such as lipidomics provides valuable strategy and approach to illustrate the complex mechanisms of TCMs. In this paper,in order to provide technical references for TCMs,we have reviewed the analytical techniques applied in lipidomics and the applications of lipidomics in TCMs researches.
引文
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[13]Scupakova K,Soons Z,Ertaylan G,et al. Spatial systems lipidomics reveals nonalcoholic fatty liver disease heterogeneity[J].Anal Chem,2018,90(8):5130.
[14]Furse S,Egmond M R,Killian J A. Isolation of lipids from biological samples[J]. Mol Membr Biol,2015,32(3):55.
[15]Zhao Z,Xu Y. An extremely simple method for extraction of lysophospholipids and phospholipids from blood samples[J]. J Lipid Res,2010,51(3):652.
[16]t'Kindt R,Jorge L,Dumont E,et al. Profiling and characterizing skin ceramides using reversed-phase liquid chromatography-quadrupole time-of-flight mass spectrometry[J]. Anal Chem,2012,84(1):403.
[17]Folch J,Lees M,Sloane Stanley G H. A simple method for the isolation and purification of total lipides from animal tissues[J].J Biol Chem,1957,226(1):497.
[18]Bligh E G,Dyer W J. A rapid method of total lipid extraction and purification[J]. Can J Biochem Physiol,1959,37(8):911.
[19]Matyash V,Liebisch G,Kurzchalia T V,et al. Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics[J].J Lipid Res,2008,49(5):1137.
[20]Lofgren L,Stahlman M,Forsberg G B,et al. The BUME method:a novel automated chloroform-free 96-well total lipid extraction method for blood plasma[J]. J Lipid Res,2012,53(8):1690.
[21]Montoliu I,Scherer M,Beguelin F,et al. Serum profiling of healthy aging identifies phospho-and sphingolipid species as markers of human longevity[J]. Aging-Us,2014,6(1):9.
[22]Ulmer C Z,Jones C M,Yost R A,et al. Optimization of Folch,Bligh-Dyer,and Matyash sample-to-extraction solvent ratios for human plasma-based lipidomics studies[J]. Anal Chim Acta,2018,1037:351.
[23]Min H K,Lim S,Chung B C,et al. Shotgun lipidomics for candidate biomarkers of urinary phospholipids in prostate cancer[J].Anal Bioanal Chem,2011,399(2):823.
[24]Panagiotopoulou P M,Tsimidou M. Solid phase extraction:applications to the chromatographic analysis of vegetable oils and fats[J]. Grasas Aceites,2002,53(1):84.
[25]Bian J,Xue Y,Yao K,et al. Solid-phase extraction approach for phospholipids profiling by titania-coated silica microspheres prior to reversed-phase liquid chromatography-evaporative light scattering detection and tandem mass spectrometry analysis[J]. Talanta,2014,123:233.
[26]Deng J,Li W,Yang Q,et al. Biocompatible surface-coated probe for in vivo,in situ,and microscale lipidomics of small biological organisms and cells using mass spectrometry[J]. Anal Chem,2018,90(11):6936.
[27]Uchikata T,Matsubara A,Fukusaki E,et al. High-throughput phospholipid profiling system based on supercritical fluid extraction-supercritical fluid chromatography/mass spectrometry for dried plasma spot analysis[J]. J Chromatogr A,2012,1250:69.
[28]Deviese T,Van Ham-Meert A,Hare V J,et al. Supercritical fluids for higher extraction yields of lipids from archeological ceramics[J]. Anal Chem,2018,90(4):2420.
[29]Truzzi C,Illuminati S,Annibaldi A,et al. Quantification of fatty acids in the muscle of antarctic fish Trematomus bernacchii by gas chromatography-mass spectrometry:optimization of the analytical methodology[J]. Chemosphere,2017,173:116.
[30]Liu Y,Chen T,Qiu Y,et al. An ultrasonication-assisted extraction and derivatization protocol for GC/TOFMS-based metabolite profiling[J]. Anal Bioanal Chem,2011,400(5):1405.
[31]Pizarro C,Arenzana-Ramila I,Perez-del-Notario N,et al. Plasma lipidomic profiling method based on ultrasound extraction and liquid chromatography mass spectrometry[J]. Anal Chem,2013,85(24):12085.
[32]Carrasco-Pancorbo A,Navas-Iglesias N,Cuadros-Rodríguez L.From lipid analysis towards lipidomics,a new challenge for the analytical chemistry of the 21st century. Part I:modern lipid analysis[J]. Tr AC Trends Anal Chem,2009,28(3):263.
[33]Putri S P,Yamamoto S,Tsugawa H,et al. Current metabolomics:technological advances[J]. J Biosci Bioeng,2013,116(1):9.
[34]Peterson B L,Cummings B S. A review of chromatographic methods for the assessment of phospholipids in biological samples[J].Biomed Chromatogr,2006,20(3):227.
[35]Ghidotti M,Fabbri D,Torri C,et al. Determination of volatile fatty acids in digestate by solvent extraction with dimethyl carbonate and gas chromatography-mass spectrometry[J]. Anal Chim Acta,2018,1034:92.
[36]Payeur A L,Lorenz M A,Kennedy R T. Analysis of fatty acid composition in insulin secreting cells by comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry[J]. J Chromatogr B Anal Technol Biomed Life Sci,2012,893-894:187.
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[38]Fukasawa K,Nakajima S,Gotoh M,et al. Qualitative and quantitative comparison of cyclic phosphatidic acid and its related lipid species in rat serum using hydrophilic interaction liquid chromatography with tandem-mass spectrometry[J]. J Chromatogr A,2018,1567:177.
[39]Choi J M,Kim T E,Cho J Y,et al. Development of lipidomic platform and phosphatidylcholine retention time index for lipid profiling of rosuvastatin treated human plasma[J]. J Chromatogr B Anal Technol Biomed Life Sci,2014,944:157.
[40]Zhang Y,Hu C,Hong J,et al. Lipid profiling reveals different therapeutic effects of metformin and glipizide in patients with type2 diabetes and coronary artery disease[J]. Diabetes Care,2014,37(10):2804.
[41]Lisa M,Cifkova E,Holcapek M. Lipidomic profiling of biological tissues using off-line two-dimensional high-performance liquid chromatography-mass spectrometry[J]. J Chromatogr A,2011,1218(31):5146.
[42]Rampler E,Schoeny H,Mitic B M,et al. Simultaneous non-polar and polar lipid analysis by on-line combination of HILIC,RP and high resolution MS[J]. Analyst,2018,143(5):1250.
[43]Yang K,Cheng H,Gross R W,et al. Automated lipid identification and quantification by multidimensional mass spectrometrybased shotgun lipidomics[J]. Anal Chem,2009,81(11):4356.
[44]Smith R,Anthonymuthu T S,Ventura D,et al. Statistical agglomeration:peak summarization for direct infusion lipidomics[J]. Bioinformatics,2013,29(19):2445.
[45]Lu L,Hu C,Zhao Y,et al. Shotgun lipidomics revealed altered profiles of serum lipids in systemic lupus erythematosus closely associated with disease activity[J]. Biomolecules,2018,8(4):105.
[46]Al Hamimi S,Sandahl M,Armeni M,et al. Screening of stationary phase selectivities for global lipid profiling by ultrahigh performance supercritical fluid chromatography[J]. J Chromatogr A,2018,1548:76.
[47]Novakova L,Perrenoud A G,Francois I,et al. Modern analytical supercritical fluid chromatography using columns packed with sub-2 mum particles:a tutorial[J]. Anal Chim Acta,2014,824:18.
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