4个薄壳山核桃品种核型分析

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英文篇名：Karyotype Analysis of Four Pecan Cultivars 作者：韩杰 ; 沈海萍 ; 储冬生 ; 吴应康 ; 彭方仁 英文作者：Han Jie;Shen Haiping;Chu Dongsheng;Wu Yingkang;Peng Fangren;Co-Innovation Center for the Sustainable Forestry in Southern China,College of Forestry,Nanjing Forestry University;Yancheng Dafeng District Forest Farm; 关键词：薄壳山核桃 ; 核型分析 ; 染色体 英文关键词：Pecan;;Karyotype analysis;;Chromosome 中文刊名：FZZW 英文刊名：Molecular Plant Breeding 机构：南京林业大学林学院南方现代林业协同创新中心;盐城市大丰区林场; 出版日期：2018-09-12 出版单位：分子植物育种 年：2018 期：v.16 基金：江苏省林业三新工程项目[LYSX[2016]44];; 国家林业局948项目(2015-4-16)共同资助 语种：中文; 页：FZZW201817036 页数：8 CN：17 ISSN：46-1068/S 分类号：210-217

摘要

薄壳山核桃是重要的木本油料作物。‘波尼’、‘马罕’、‘金华’、‘钟山25’都是中国大面积推广种植的优良品种。本研究采用常规染色体压片法,以薄壳山核桃嫩芽为材料,对该4个主要栽培品种进行核型分析。结果表明,薄壳山核桃的染色体为2n=32。4个无性系品种的核型类型都是2B。‘波尼’染色体组成为2S+16M1+12M2+2L,核型公式为2n=32=12sm+18m+2T;‘马罕’染色体组成为6S+12M1+8M2+6L,核型公式为2n=32=16sm+14m+2T;‘金华’染色体组成为4S+12M1+14M2+2L,核型公式为2n=32=10sm+20m+2T;‘钟山25’染色体组成为6S+12M1+8M2+6L,核型公式为2n=32=8sm+22m+2T。4个无性系品种的核型不对称系数分别为62.07%、64.68%、62.47%、62.38%。从进化角度来看,‘波尼’属于比较原始的类型,‘马罕’属于比较进化的类型。本研究可为薄壳山核桃种质资源鉴定和育种提供细胞学基础。
        Pecan(Carya illinoinensis) is an important woody oil crop.'Pawnee','Mahan','Jinhua','Zhongshan25'are all fine varieties that have been promoted to cultivate in large scale in China.The chromosome numbers and karyotypes of four pecan cultivars were studied by applying traditional squash technique.The results showed that the chromosome number of all tested cultivars was 32.The karyotypes of four kinds of Carya illinoinensis were2 B.The results indicated that the karyotype formula of 'Pawnee' was 2 n=32=12 sm+18 m+2 T,chromosome composition of relative length was 2 S+16 M1+12 M2+2 L;and the karyotype formula of 'Mahan' was 2 n=32=16 sm+14 m+2 T,chromosome composition of relative length was 6 S+12 M1+8 M2+6 L;as to 'Jinhua',the karyotype formula was 2 n=32=10 sm+20 m+2 T,the chromosome composition of relative length was 4 S+12 M1+14 M2+2 L,the last one is 'Zhongshan25',the karyotype formula was 2 n=32=8 sm+22 m+2 T,the chromosome composition of relative length was 6 S+12 M1+8 M2+6 L.These index of kary otype asymmetries of four pecan cultivars were62.07%,64.68%,62.47%,62.38%.'Pawnee' was relative original while 'Mahan' was evolutional during genome evolution.This study could provide cytological basis for the identification and breeding of germplasm resource of pecan.

引文

Arano H.,1963,Cytological studies in subfamily Carduoideae(Compositae)of Japan IX.The karyotype analysis and phylogenic considerations on pertya and ainsliaea(2),Shokubutsugaku Zasshi,76(895):32-39
    Huang S.S.,Mo X.L.,Zeng Q.Q.,Cai Y.W.and Qiu W.F.,2009,Analysis on karyotype of Melocanna humilis Kurz,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),28(4):751-754(黄珊珊,莫小路,曾庆钱,蔡岳文,邱蔚芬,2009,小梨竹核型分析,基因组学与应用生物学,28(4):751-754)
    Kuo S.R.,Wang T.T.,and Huang T.C.,1972,Karyotype analysis of some Foromsan gymnosperms,Taiwania,17(1):66-80
    Li J.N.,Li L.F.,Xiong X.W.,Chen H.W.,and Xi X.L.,2012,Research progress of asexual propagation techniques of Carya illinoensis,Anhui Nongye Kexue(Journal of Anhui Agricultural Sciences),40(33):16226-16228(李俊南,李莲芳,熊新武,陈宏伟,习学良,2012,薄壳山核桃无性繁殖技术研究进展,安徽农业科学,40(33):16226-16228)
    Li M.X.,and Chen R.Y.,1985,A suggestion on the standardization of karyotype analysis in plants,Wuhan Zhiwuxue Yanjiu(Journal of Wuhan Botanical Research),3(4):297-302(李懋学,陈瑞阳,1985,关于植物核型分析的标准化问题,武汉植物学研究,3(4):297-302)
    Lima-de-Faria A.,1980,Classification of genes,rearrangements and chromosomes according to the chromosome field,Hereditas,93(1):1-46
    Lombardini L.,2006,One-time pruning of pecan trees induced limited and short-term benefits in canopy light penetration,yield,and nut quality,Hortscience,41(6):1469-1473
    Lv F.D.,Yang F.,and Zhang R.Q.,2002,Karyotypes of three Carya Nutt.species,Zhongnan Linxueyuan Xuebao(Journal of Central South Forestry University),22(1):47-49(吕芳德,杨帆,张日清,2002,山核桃属部分种的核型分析,中南林学院学报,22(1):47-49)
    Ma M.C.,Wang Y.,Gao H.Q.,and Zhuang N.S.,2017,Karyotype analysis of three varieties in Hevea brasiliensis,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),15(4):1563-1568(马梦晨,王英,高和琼,庄南生,2017,橡胶树三个品种的核型分析,分子植物育种,15(4):1563-1568
    Ma M.R.,Wang Y.,Zhuang N.S.,and Gao H.Q.,2017,Kary otype analysis of four main rubber tree(Hevea brasiliensis)cultivars,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),36(1):311-317(马梦茹,王英,庄南生,高和琼,2017,四个橡胶树主要栽培品种的核型分析,基因组学与应用生物学,36(1):311-317)
    Stebbins G.L.,ed.,1971,Chromosomal evolution in higherplants,Addison-Wesley,London,England,pp.1-21
    Wang L.P.,2010,Karyotype analysis on five species of genus Buxus,Thesis for M.S.,Nanjing Forestry University,Supervisor:Ji K.S.,pp.13-15(王丽平,2010,黄杨属五种植物的核型分析,硕士学位论文,南京林业大学,导师:季孔庶,pp.13-15)
    Yang F.,2001,Karyotype of Carya illinoensis and Carya tonkinensis,Jiangxi Linye Keji(Jiangxi Forestry Science and Technology),(2):6-7(杨帆,2001,薄壳山核桃及云南山核桃染色体核型分析,江西林业科技,(2):6-7)
    Zhang R.Q.,and Lv F.D.,2002,Pecan distribution,cultural Regionalizationand and cultivar classification in USA,Jingjilin Yanjiu(Economic forest Researches),20(3):53-55(张日清,吕芳德,2002,美国山核桃在原产地分布、引种栽培区划及主要栽培品种分类研究概述,经济林研究,20(3):53-55)
    Zhang R.Q.,Lv F.D.,and He F.,2001,The pecan tree and its adaptability in China,Jiangsu Linye Keji(Journal of Jiangsu Forestry Science&Technology),28(4):45-47(张日清,吕芳德,何方,2001,美国山核桃及其在我国的适应性研究,江苏林业科技,28(4):45-47)
    Zhou Z.,and Gu S.Y.,2010,Image processing of karyotype analysis,Shengwuxue Zazhi(Journal of Biology),27(6):95-96(周洲,顾曙余,2010,核型分析实验中的图像处理,生物学杂志,27(6):95-96)
    Zhu H.J.,Liu G.Q.,Cao F.L.,Zang X.,Zhou B.B.,and Sheng J.Y.,2012,Effects of zinc application on seedling growth and zinc distribution in different or gans of Carya illinoensis(Wangenh.)K.Koch,Nanjing Linye Daxue Xuebao(Journal of Nanjing Forestry University(Natural Sciences Edition)),36(4):75-78(朱海军,刘广勤,曹福亮,臧旭,周蓓蓓,生静雅,2012,施锌对薄壳山核桃幼苗生长及体内锌分配的影响,南京林业大学学报(自然科学版),36(4):75-78)