改良石蜡切片法观察金针菇子实体不同发育时期组织结构
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摘要
运用改良石蜡切片法制备了金针菇(Flammulina filiformis)子实体不同发育时期的切片,观察其菌褶和菌柄的细胞结构。结果表明,以柠檬烯为透明剂,金针菇子实体组织结构清晰、完整、无皱缩。观察不同发育时期的金针菇子实体组织切片发现,金针菇原基内部有呈菌丝无规则扭结状态的菌盖结构,随着子实体不断发育,菌盖下端有菌褶结构分化,随后菌柄结构随菌盖发育而进一步分化,菌柄组织细胞状纵向紧密排列,横切面有维管束状细胞呈外紧密内疏松状排列且其间有孔洞出现,菌褶菌髓部呈菌丝与球泡状细胞共同存在的异型分布结构。
The improved paraffin method was used to prepare sections of Flammulina filiformis fruiting bodies at different developmental stages,and to observe cell structure of gills and stipes.The results showed that F.filiformis fruiting body section was clear,intact and wrinkle-free with limonene as clearing agent.Observation of tissue sections of F.filiformis fruiting bodies showed that there was a pileus structure with irregularly twisted mycelium in primordium.With the development of fruiting body,the gills differentiated in the bottom of pileus; after that,the stipe structure was further differentiated with the development of pileus.The stipe cells were longitudinally closely aligned in F.filiformis.Viewing from the cross section,vascular bundle cells were arranged as compacting inside and loosening outside with cavities in it.A heteromorphic structure was observed,where mycelia and bulliform cells coexisted in gill trama.
The improved paraffin method was used to prepare sections of Flammulina filiformis fruiting bodies at different developmental stages,and to observe cell structure of gills and stipes.The results showed that F.filiformis fruiting body section was clear,intact and wrinkle-free with limonene as clearing agent.Observation of tissue sections of F.filiformis fruiting bodies showed that there was a pileus structure with irregularly twisted mycelium in primordium.With the development of fruiting body,the gills differentiated in the bottom of pileus; after that,the stipe structure was further differentiated with the development of pileus.The stipe cells were longitudinally closely aligned in F.filiformis.Viewing from the cross section,vascular bundle cells were arranged as compacting inside and loosening outside with cavities in it.A heteromorphic structure was observed,where mycelia and bulliform cells coexisted in gill trama.
引文
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[20]REIJNDERS AFM.On the origin of specialized trama types in the Agaricales[J].Mycological Research,1993,97(3):257-268.
[2]杨和川,谭一罗,苏文英,等.金针菇菌株农艺性状评价及遗传多样性分析[J].南方农业学报,2018,49(12):2371-2378.
[3]龙莹,严俊杰,仝宗军,等.fv-gs6:一个与金针菇菌柄伸长相关的葡聚糖合酶基因[J].福建农林大学学报(自然科学版),2018,47(6):698-704.
[4]丁霄霄,李凤伟,商曰玲,等.金针菇液态发酵培养初步研究[J].中国酿造,2018,37(9):124-129.
[5]WILLIAMS MAJ,BECKETT A,READ ND.Ultrastructural aspects of fruit body differentiation in Flammutina velutipes.In:Developmental Biology of Higher Fungi[M].Cambridge:Cambridge University Press,1985.
[6]赵亚林,闫青地,冯佳佳,等.石蜡切片方法的改良[J].安徽农业科学,2017,45(32):6-8.
[7]宋超.半被果型担子果发育研究[D].长春:吉林农业大学,2008.
[8]张鹏.木耳形态发育及木耳属次生菌丝和子实体的解剖学研究[D].长春:吉林农业大学,2011.
[9]杨虎彪,李晓霞,罗丽娟.植物石蜡制片中透明和脱蜡技术的改良[J].植物学报,2009,44(2):230-235.
[10]ARGALL K,ARMATI P.Use of microwave fixation in the preparation of cell cultures for observation with the scanning electron microscope[J].Journal of Electron Microscopy Technique,1990,16(4):347.
[11]LANGMAN JM.d-Limonene:Is it a safe,effective alternative to xylene?[J].Journal of Histotechnology,1995,18(2):131-137.
[12]张天杰,谢明,唐玉娇,等.正丁醇作透明剂在病理组织切片中的应用[J].湘南学院学报(医学版),2014,16(4):65-67.
[13]宋超,图力古尔.双孢蘑菇子实体发育研究[J].中国食用菌,2012,31(6):11-13.
[14]杨桂梅.金针菇工厂化栽培管理[J].安徽农业科学,2013,41(36):13867-13868.
[15]邓伦武,黄文静,何海旺,等.Steedman's wax包埋切片DAPI染色法证实铝诱导花生根尖细胞程序性死亡[J].南方农业学报,2014,45(8):1353-1357.
[16]田子强,刘俊峰,张少为,等.普通甲醛固定石蜡包埋组织DNA提取方法的探讨[J].癌症,2004,23:342-345.
[17]辛颖,WERTHER GA,RUSSO VC.新生大鼠缺氧缺血后不同脑区神经元变性的动态观察[J].中国医科大学学报,2006,35(1):1-3.
[18]盖宇鹏.球盖菇科几个种的个体发育研究[D].长春:吉林农业大学,2012.
[19]GINNS J,REIJNDERS AFM,STAPLERS JA.The Development of the Hymenophoral Trama in the Aphyllophorales and the Agaricales[J].Mycologia,1992,85(4):718.
[20]REIJNDERS AFM.On the origin of specialized trama types in the Agaricales[J].Mycological Research,1993,97(3):257-268.
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