番茄晚疫病拮抗细菌FT-1菌株的筛选及鉴定
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摘要
为筛选出对番茄晚疫病有较好防效的拮抗细菌,从健康番茄根际采集8份土样,分离纯化得到54株菌株,经平板对峙法筛选出1株对番茄晚疫病病原菌拮抗效果较好的菌株FT-1,通过生理生化试验、形态特征观察对其进行了种属初步鉴定,鉴定结果为菌株FT-1属于芽孢杆菌属。
In order to obtain antagonistic bacteria against Phtophthora infestans( Mont.) De Bury,about 54 strains of bacteria isolated from 8 soil samples were screened with the agar plate diffusion method. After primary and secondary screening,the strain FT-1 showed the most significant antagonistic activity. After the morphological observation and a series of physiological and biochemistry experiments,the bacterial strain FT-1 wasprimarily identified as Bacillus sp.
In order to obtain antagonistic bacteria against Phtophthora infestans( Mont.) De Bury,about 54 strains of bacteria isolated from 8 soil samples were screened with the agar plate diffusion method. After primary and secondary screening,the strain FT-1 showed the most significant antagonistic activity. After the morphological observation and a series of physiological and biochemistry experiments,the bacterial strain FT-1 wasprimarily identified as Bacillus sp.
引文
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[2]Becktell M C,Daughtery M L,Fry W E.Epidemiology and Manage ment of Pet Unia and Tomato Late Blight[J].Plant Disease Sep,2005,89(9):1000-1008.
[3]安德荣.生物制药的原理及方法一抗生素的制备[M].北京:中国科学文化出版社,2002
[4]方中达.植病研究法[M].北京:农业出版社,1977:161-224.
[5]Niemann H,Losekann T,de Beer D,et al.Novel microbial communities of the Haakon Mosby mud volcano and their role as a methane sink[J].Nature,2006,443(5227):854-858.
[6]赵斌,何绍江.微生物学实验[M].北京:科学出版社,2002:86
[7]李春杰,刘海龙,许艳丽.大豆根腐病拮抗细菌的生物学特性研究[J].大豆科学,2011,30(5):809-812.
[8]东秀珠,蔡妙英.常用细菌系统鉴定手册[M].北京:科学出版社,2001:399-417.
[9]布坎南R E,吉本斯N E.伯杰细菌鉴定手册[M].北京:科学出版社,1984
[10]Qin GZ,Tian SP.Biocontrol of postharvest diseases of jujube fruit by Criptococcus laurentii combined with a low dosage of fungicides under difherent storage conditions[J].Plant Disease,2004,88(5):497-501.[l1]Zhou DQ.Laboratory Manual of Microbiology[M].Shanghai:Shanghai Scientific and Technical Publishers,1986:40-42.
[12]蒋继志,赵丽坤,史娟,等.儿种真菌发酵液对致病疫霉的抑制作用[J].微生物学通报,2001,28(2):55-59.
[13]郭会靖,蒋继志,李向彬,等.致病疫霉拮抗菌筛选及复合发酵液的抑菌作用研究[J].河北农业大学学报,2008,31(4):87-90.
[14]蒋继志,李莎,工会仙.3株致病疫霉拮抗放线菌混合发酵及其抑制机理[J].河北人学学报,2010,30(1):78-82.
[15]Liu D,Anderson N A,Kmkel L.Selection and characterization of suppressive strains of Streptomyces inhibiting potato scab[J].Canada Journal of Plant Pathology,1996,42(5):487-502.
[16]刘静,王军,姚建铭,等.枯草芽抱杆菌扒抗菌物特性的研究及抗菌肤的分离纯化[J].微生物学报,2004,44(4):511-513.
[17]Maget D R,Peypoux F.Iturins a special class of pore forming lipopeptides biological and hysicochemical properties[J].Toxicology,1994,87(2):151-174.