基于可逆共价酰腙键制备海藻酸水凝胶及其pH响应性研究
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摘要
利用醛基化海藻酸钠与酰肼基聚乙二醇反应,获得动态共价键交联的海藻酸水凝胶。通过核磁共振光谱、红外光谱、扫描电镜对水凝胶的结构进行了表征,采用流变仪测定水凝胶的流变性能。结果表明该水凝胶具有较高的弹性和强度,并且随pH值的变化,可以实现溶胶-凝胶转化,即在中性条件下形成凝胶,在酸性条件下则变为溶胶。基于这种优异的性能,再加上优良的生物相容性,将为此类材料在药学生物领域的应用打下坚实的基础。
Aldehyde-based alginate was reacted with hydrazide-based PEG to obtain a dynamical covalent crosslinked alginate hydrogel.The structure of hydrogel was characterized by 1 H-NMR,FT-IR and SEM.The rheological properties were measured by rheometer.The results showed that the hydrogel had high elasticity and strength,and with the change of pH value,sol-gel conversion can be realized which was the gel forms under neutral conditions and the sol under acidic conditions.Based on this excellent performance,combined with excellent biocompatibility the hydrogel will lay a solid foundation for the application of such materials in the field of pharmaceutical biology.
Aldehyde-based alginate was reacted with hydrazide-based PEG to obtain a dynamical covalent crosslinked alginate hydrogel.The structure of hydrogel was characterized by 1 H-NMR,FT-IR and SEM.The rheological properties were measured by rheometer.The results showed that the hydrogel had high elasticity and strength,and with the change of pH value,sol-gel conversion can be realized which was the gel forms under neutral conditions and the sol under acidic conditions.Based on this excellent performance,combined with excellent biocompatibility the hydrogel will lay a solid foundation for the application of such materials in the field of pharmaceutical biology.
引文
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[2]Mladenovska K,Raicki R S,Janevik E I,et al.Colon-specific delivery of 5-aminosalicylic acid from chitosan-Ca-alginate microparticles[J].International Journal of Pharmaceutics,2007,342(1-2):124-136.
[3]Hall K K,Gattas-Asfura K M,Stablfr C L.Microencapsulation of islets within alginate/poly(ethylene glycol)gels crosslinked via staudinger ligation[J].Acta Biomaterialia,2011,7(2):614-624.
[4]Miao T X,Rao K S,Spees J L,et al.Osteogenic differentiation of human mesenchymal stem cells through alginate-graft-poly(ethylene glycol)microsphere-mediated intracellular growth factor delivery[J].Journal of Controlled Release,2014,192:57-66.
[5]Jeon O,Samorezov J E,Alsberg E.Single and dual crosslinked oxidized methacrylated alginate/PEG hydrogels for bioadhesive applications[J].Acta Biomaterialia,2014,10(1):47-55.
[6]Sun J Y,Zhao X H,Llleperuma W R K,et al.Highly stretchable and tough hydrogels[J].Nature,2012,489(7414):133-136.
[7]Balakrishnan B J,Jayakrishnan A.Self-cross-linking biopolymers as injectable in situ forming biodegradable scaffolds[J].Biomaterials,2005,26(18):3941-3951.
[8]Han X J,Dong Z Q,Fan M M,et al.pH-inducde shape-memory polymers[J].Macromolecular Rapid Communications,2012,33(12):1055-1060.
[9]Ding F Y,Wu S P,Wang S S,et al.A dynamic and selfcrosslinked polysaccharide hydrogel with autonomous selfhealing ability[J].Soft Matter,2015,11(20):3971-3976.
[10]Deng G H,Tang C M,Li F Y,et al.Covalent cross-linked polymer gels with reversible sol-gel transition and self-healing properties[J].Macromolecules,2010,43(3):1191-1194.
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[12]Wu N Q,Pan C Y,Zhang B J,et al.Preparation and properties of a thermo-sensitive hydrogel based on oxidized sodium alginate[J].Acta Polymerica Sinica,2007,6:497-502.