醋延胡索饮片颜色与其内在质量的相关性分析
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摘要
目的:基于国际照明委员会(CIE)LAB颜色空间技术对醋延胡索饮片颜色数据化,结合饮片中10种主要生物碱类成分的含量,探讨醋延胡索饮片颜色与主要成分含量的相关性,以及不同颜色饮片的内在质量差异。方法:采用精密色差仪测定醋延胡索饮片颜色参数;采用HPLC测定醋延胡索饮片中主要化学成分含量,Agilent ZORBAX SB-C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相乙腈(A)-0.1%磷酸二氢钾水溶液(B)梯度洗脱(0～10 min,5%～22%A;10～30 min,22%～25%A;30～50 min,25%～60%A;50～70 min,60%～95%A),检测波长280 nm,柱温25℃,流速1.0 m L·min~(-1),进样量10μL。结果:不同颜色醋延胡索饮片质量均符合2015年版《中国药典》的要求,但饮片之间内在质量存在差异,样品中所测化学成分总量与CIELAB颜色空间内a~*(红绿轴)和总色差值(ΔE)呈正相关性,与L~*(明度),b~*(黄蓝轴)具显著负相关性;所测成分中除D-四氢药根碱与四氢黄连碱外,延胡索丙素等7种成分的含量与颜色呈正相关,只有延胡索甲素含量与颜色呈负相关。结论:色彩分析技术不仅可以客观量化饮片颜色,还可通过分析颜色与主要有效成分含量的相关性,实现饮片质量的快捷评价。
Objective:Based on the color space technology of CIE-LAB,the color of vinegar-processed Corydalis Rhizoma decoction pieces was digitized,combining with the contents of 10 major alkaloids in the decoction pieces,to discuss the correlation between the color and contents of main ingredients of vinegar-processed Corydalis Rhizoma decoction pieces,and investigate the intrinsic quality difference in the decoction pieces with different color.Method:The precision colorimeter was used to determine the color parameters of vinegar-processed Corydalis Rhizoma decoction pieces;HPLC was employed to determine contents of main chemical components in the decoction pieces,which was performed on Agilent ZORBAX SB-C_(18)column(4.6 mm×250 mm,5μm)with mobile phase of acetonitrile(A)-0.1%potassium dihydrogen phosphate aqueous solution(B)for gradient elution(0-10 min,5%-22%A;10-30 min,22%-25%A;30-50 min,25%-60%A;50-70 min,60%-95%A),detection wavelength of 280 nm,column temperature at 30℃and flow rate of 1.0 m L·min~(-1).Result:The quality of vinegar-processed Corydalis Rhizoma decoction pieces with different color was in line with the requirement of the 2015 edition of Chinese Pharmacopoeia,but there were differences in the intrinsic quality between the decoction pieces.The total content of chemical components in the samples showed a positive correlation with the a~*(green-red axis)and total chromatic aberration value(ΔE)in the CIE-LAB color space,and it was significantly negative correlated with L~*(lightness)and b~*(blue-yellow axis).In the 10 tested components,except for D-tetrahydrojatrorrhizine and tetrahydrocoptisine,contents of protopine and other 6 components were positively correlated with color,and only the content of corydaline was negatively correlated with color.Conclusion:Color analysis technology can objectively quantify the color of the decoction pieces,and can achieve a quick evaluation of quality of the decoction pieces by analyzing correlation between the color and the contents of main active ingredients.
Objective:Based on the color space technology of CIE-LAB,the color of vinegar-processed Corydalis Rhizoma decoction pieces was digitized,combining with the contents of 10 major alkaloids in the decoction pieces,to discuss the correlation between the color and contents of main ingredients of vinegar-processed Corydalis Rhizoma decoction pieces,and investigate the intrinsic quality difference in the decoction pieces with different color.Method:The precision colorimeter was used to determine the color parameters of vinegar-processed Corydalis Rhizoma decoction pieces;HPLC was employed to determine contents of main chemical components in the decoction pieces,which was performed on Agilent ZORBAX SB-C_(18)column(4.6 mm×250 mm,5μm)with mobile phase of acetonitrile(A)-0.1%potassium dihydrogen phosphate aqueous solution(B)for gradient elution(0-10 min,5%-22%A;10-30 min,22%-25%A;30-50 min,25%-60%A;50-70 min,60%-95%A),detection wavelength of 280 nm,column temperature at 30℃and flow rate of 1.0 m L·min~(-1).Result:The quality of vinegar-processed Corydalis Rhizoma decoction pieces with different color was in line with the requirement of the 2015 edition of Chinese Pharmacopoeia,but there were differences in the intrinsic quality between the decoction pieces.The total content of chemical components in the samples showed a positive correlation with the a~*(green-red axis)and total chromatic aberration value(ΔE)in the CIE-LAB color space,and it was significantly negative correlated with L~*(lightness)and b~*(blue-yellow axis).In the 10 tested components,except for D-tetrahydrojatrorrhizine and tetrahydrocoptisine,contents of protopine and other 6 components were positively correlated with color,and only the content of corydaline was negatively correlated with color.Conclusion:Color analysis technology can objectively quantify the color of the decoction pieces,and can achieve a quick evaluation of quality of the decoction pieces by analyzing correlation between the color and the contents of main active ingredients.
引文
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[8]张铁军,许浚,申秀萍,等.基于中药质量标志物(QMarker)的元胡止痛滴丸的“性-效-物”三元关系和作用机制研究[J].中草药,2016,47(13):2199-2211.
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[10]岳显可,朱涛,顾超,等.不同等级延胡索药材多指标成分含量测定及指纹图谱分析[J].中华中医药学刊,2016,34(12):2868-2874.
[11]顾超,岳显可,杜伟锋,等.延胡索中3种成分近红外同步定量检测模型的建立[J].中国实验方剂学杂志,2017,23(3):60-65.
[12]吕秋菊,秦海燕,宋捷民,等.延胡索药材商品规格等级划分的合理性研究[J].甘肃中医药大学学报,2017,34(2):70-76.
[2]李玉山.延胡索乙素的制备工艺及药理作用研究进展[J].解放军药学学报,2013,29(5):480-483.
[3]王文凯,徐彭,杨武亮,等.不同酸制延胡索的实验研究[J].中国实验方剂学杂志,2011,17(3):26-28.
[4]徐婷,金昔陆,曹惠明.延胡索乙素药理作用的研究进展[J].中国临床药学杂志,2001,10(1):58-60.
[5]张凯丽,王绩英.去氢紫堇碱的药理作用及其相关研究进展[J].世界最新医学信息文摘,2018,18(16):120-122.
[6]侯晓琳,邱智东.延冰片的化学成分及药理作用研究进展[J].长春中医药大学学报,2016,32(6):1306-1308.
[7]张铁军,许浚,韩彦琪,等.中药质量标志物(Qmarker)研究:延胡索质量评价及质量标准研究[J].中草药,2016,47(9):1458-1467.
[8]张铁军,许浚,申秀萍,等.基于中药质量标志物(QMarker)的元胡止痛滴丸的“性-效-物”三元关系和作用机制研究[J].中草药,2016,47(13):2199-2211.
[9]孙婷婷,龚千锋,李娆娆,等.延胡索指标性成分延胡索乙素的影响因素分析[J].中国实验方剂学杂志,2013,19(3):89-92.
[10]岳显可,朱涛,顾超,等.不同等级延胡索药材多指标成分含量测定及指纹图谱分析[J].中华中医药学刊,2016,34(12):2868-2874.
[11]顾超,岳显可,杜伟锋,等.延胡索中3种成分近红外同步定量检测模型的建立[J].中国实验方剂学杂志,2017,23(3):60-65.
[12]吕秋菊,秦海燕,宋捷民,等.延胡索药材商品规格等级划分的合理性研究[J].甘肃中医药大学学报,2017,34(2):70-76.