早期不同负荷运动对后期2型糖尿病大鼠成模率及血糖水平的影响
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摘要
背景:糖代谢紊乱是糖尿病的主要临床表现,运动是治疗糖尿病的主要方法之一,早期不同负荷运动对后期2型糖尿病大鼠模型成模率及血糖水平的影响尚不明确。目的:探究不同负荷运动对高糖高脂膳食联合注射链脲佐菌素建立大鼠2型糖尿病模型的影响。方法:50只SD雄性大鼠(购自湖北省实验动物研究中心),随机分为安静对照组、糖尿病对照组、小强度运动干预组、中等强度运动干预组和大强度运动干预组5组,每组10只。安静对照组普通饲料喂养;糖尿病对照组高糖高脂饲料喂养,均笼中自由活动;小、中、大强度运动干预组高糖高脂喂养,期间分别进行8周不同强度的跑台运动。运动第8周末,检测各组大鼠的体质量和空腹血糖,然后隔夜禁食不禁水12 h,安静对照组一次性腹腔注射柠檬酸缓冲液,其余4组一次性腹腔注射2%链脲佐菌素溶液。注射链脲佐菌素第7天再次检测大鼠体质量、空腹血糖和糖化血清蛋白水平。结果与结论:不同强度运动干预组的体质量、空腹血糖、糖化血清蛋白、血糖曲线下面积均较糖尿病对照组有所改善,中等、大强度运动干预可更有效地调节2型糖尿病血糖水平,改善糖耐量,而大强度运动干预对于降低2型糖尿病的发病率更为有效。
BACKGROUND: Dysfunction of glucose metabolism is the main clinical manifestation of diabetes mellitus.Exercise is one of the main methods to treat diabetes mellitus. The effect of early exercise with different loads on the modeling rate and blood glucose level of late-stage type 2 diabetic rats is unclear. OBJECTIVE: To explore the effects of different exercise loads on the establishment of rat diabetes mellitus model with high-sugar and high-fat diet combined with streptozotocin injection. METHODS: Fifty Sprague-Dawley male rats(provided by Hubei Provincial Laboratory Animal Research Center) were randomly divided into quiet, diabetes mellitus, and low-, moderate-, and high-intensity exercise groups(n=10/group). Rats in the quiet group were fed with normal feed, diabetes mellitus group were fed with high-sugar and high-fat diets, and were freely active in cages. Mice in the low-, moderate-, and high-intensity exercise groups were fed with high-sugar and high-fat diets, and underwent 8-week exercise of different intensities. At the end of exercise, the body weight and free blood glucose in each group were tested, and then the rats were fasted but free to drink for 12 hours overnight. Quiet group was given intraperitoneal injection of citrate buffer, and other groups were injected intraperitoneally with 2% streptozotocin solution. The body weight, free blood glucose and glycated serum protein on day 7 were detected. RESULTS AND CONCLUSION: The body weight, free blood glucose, glycated serum protein, and area under the curve of blood glucose in each exercise group were improved compared with the diabetes mellitus group. Moderate-and high-intensity exercise can effectively regulate blood glucose level and improve glucose tolerance of type 2 diabetes mellitus. Besides, high-intensity exercise is more effective in reducing the incidence of type 2 diabetes mellitus.
BACKGROUND: Dysfunction of glucose metabolism is the main clinical manifestation of diabetes mellitus.Exercise is one of the main methods to treat diabetes mellitus. The effect of early exercise with different loads on the modeling rate and blood glucose level of late-stage type 2 diabetic rats is unclear. OBJECTIVE: To explore the effects of different exercise loads on the establishment of rat diabetes mellitus model with high-sugar and high-fat diet combined with streptozotocin injection. METHODS: Fifty Sprague-Dawley male rats(provided by Hubei Provincial Laboratory Animal Research Center) were randomly divided into quiet, diabetes mellitus, and low-, moderate-, and high-intensity exercise groups(n=10/group). Rats in the quiet group were fed with normal feed, diabetes mellitus group were fed with high-sugar and high-fat diets, and were freely active in cages. Mice in the low-, moderate-, and high-intensity exercise groups were fed with high-sugar and high-fat diets, and underwent 8-week exercise of different intensities. At the end of exercise, the body weight and free blood glucose in each group were tested, and then the rats were fasted but free to drink for 12 hours overnight. Quiet group was given intraperitoneal injection of citrate buffer, and other groups were injected intraperitoneally with 2% streptozotocin solution. The body weight, free blood glucose and glycated serum protein on day 7 were detected. RESULTS AND CONCLUSION: The body weight, free blood glucose, glycated serum protein, and area under the curve of blood glucose in each exercise group were improved compared with the diabetes mellitus group. Moderate-and high-intensity exercise can effectively regulate blood glucose level and improve glucose tolerance of type 2 diabetes mellitus. Besides, high-intensity exercise is more effective in reducing the incidence of type 2 diabetes mellitus.
引文
[1]中华医学会糖尿病学分会.中国2型糖尿病防治指南(2017年版)[J].中华糖尿病杂志,2018,10(1):4-67.
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[3]李源源,方奋,贾占昀,等.不同强度有氧运动对2型糖尿病患者血糖及心肺耐力的影响[J].中华物理医学与康复杂志, 2017,39(8)617-619.
[4]罗曦娟,张献博,徐峻华,等.运动是良医应用实例:美国糖尿病预防项目及其应用[J].北京体育大学学报, 2016,39(8):59-65,73.
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[6]马晓雯,常芸,王世强,等.长期耐力训练对大鼠心肌细胞自噬相关因子Beclin1和LC3的影响[J].体育科学, 2016,36(2):66-71.
[7]余丹,叶山东,李业琼,等.二甲双胍对2型糖尿病模型大鼠肾组织蛋白激酶C-β表达的影响[J].中国糖尿病杂志, 2017,25(10):904-908.
[8]王晓凌,姜玲玲,邢立勇,等.高脂饲养诱导大鼠胰岛素抵抗的动态观察[J].中国老年学杂志, 2008,28(7):664-666.
[9]韩芸,梁伟玲,吴君,等.海洋多糖多肽复合物对2型糖尿病大鼠糖耐量的影响[J].现代中西医结合杂志, 2016,25(2):141-143,154.
[10]王胜男,罗利娟,陈毅,等.运动和食物成分对3周龄雄性小鼠体重调节的影响[J].中国运动医学杂志, 2018,37(2):152-157.
[11]杨京辉,汪亚群,楼青青,等.高强度间歇运动对糖尿病前期患者糖脂代谢的影响[J].中国康复医学杂志, 2017,32(8):907-911,937.
[12]郭彩红,高秀莹.达格列净对2型糖尿病伴代谢综合征患者代谢指标影响[J].临床军医杂志, 2018,46(5):585-587.
[13]沈亚非,徐焱成.高糖高脂膳食和链脲佐菌素诱导2型糖尿病模型的建立[J].实用诊断与治疗杂志, 2006,20(9):649-650.
[14]许冬明,李显国.高脂饮食及运动对2型糖尿病大鼠脂联素、瘦素及血糖水平的影响[J].中国应用生理学杂志, 2016,32(4):298-300.
[15]杨丽丽,董建成,卞尧尧,等.果糖胺检测对糖尿病诊断价值的Meta分析[J].中国糖尿病杂志, 2015,23(7):621-626.
[16]Roberts CK, Little JP, Thyfault JP. Modification of insulin sensitivity and glycemic control by activity and exercise. Med Sci Sports Exerc. 2013;45(10):1868-1877.
[17]邵月.耐力运动对糖尿病大鼠血清血糖、胰岛素、胰岛素抵抗指数、糖化血清蛋白、红细胞糖化血红蛋白的影响[J].中国老年学杂志, 2010,30(1):54-56.
[18]Khan S, Jena G. Sodium butyrate reduces insulin-resistance,fat accumulation and dyslipidemia in type-2 diabetic rat:A comparative study with metformin. Chem Biol Interact. 2016;254:124-134.
[19]Garabadu D, Krishnamurthy S. Metformin attenuates hepatic insulin resistance in type-2 diabetic rats through PI3K/Akt/GLUT-4 signalling independent to bicuculline-sensitive GABAA receptor stimulation. Pharm Biol. 2017;55(1):722-728.
[20]江洪.不同运动强度干预2型糖尿病模型大鼠的骨密度变化[J].中国组织工程研究, 2016,20(27):3963-3969.
[21]姜得悦,李婷,田晨光,等.二甲双胍抑制2型糖尿病大鼠脂肪组织胎球蛋白A表达的研究[J].中国糖尿病杂志, 2018,26(8):671-675.
[22]赵云鹏.中等强度有氧运动对2型糖尿病患者胰岛素抵抗相关因素的影响[J].广州体育学院学报, 2016,36(4):106-108.
[23]Jiménez-Maldonado A, Virgen-Ortiz A, Melnikov V, et al.Effect of moderate and high intensity chronic exercise on the pancreatic islet morphometry in healthy rats:BDNF receptor participation. Islets. 2017;9(1):1-10.
[2]耿雪,申晋波.不同运动方式干预2型糖尿病机制的研究进展[J].体育科研, 2018,39(2):91-99.
[3]李源源,方奋,贾占昀,等.不同强度有氧运动对2型糖尿病患者血糖及心肺耐力的影响[J].中华物理医学与康复杂志, 2017,39(8)617-619.
[4]罗曦娟,张献博,徐峻华,等.运动是良医应用实例:美国糖尿病预防项目及其应用[J].北京体育大学学报, 2016,39(8):59-65,73.
[5]Bedford TG, Tipton CM, Wilson NC, et al. Maximum oxygen consumption of rats and its changes with various experimental procedures. J Appl Physiol Respir Environ Exerc Physiol. 1979;47(6):1278-1283.
[6]马晓雯,常芸,王世强,等.长期耐力训练对大鼠心肌细胞自噬相关因子Beclin1和LC3的影响[J].体育科学, 2016,36(2):66-71.
[7]余丹,叶山东,李业琼,等.二甲双胍对2型糖尿病模型大鼠肾组织蛋白激酶C-β表达的影响[J].中国糖尿病杂志, 2017,25(10):904-908.
[8]王晓凌,姜玲玲,邢立勇,等.高脂饲养诱导大鼠胰岛素抵抗的动态观察[J].中国老年学杂志, 2008,28(7):664-666.
[9]韩芸,梁伟玲,吴君,等.海洋多糖多肽复合物对2型糖尿病大鼠糖耐量的影响[J].现代中西医结合杂志, 2016,25(2):141-143,154.
[10]王胜男,罗利娟,陈毅,等.运动和食物成分对3周龄雄性小鼠体重调节的影响[J].中国运动医学杂志, 2018,37(2):152-157.
[11]杨京辉,汪亚群,楼青青,等.高强度间歇运动对糖尿病前期患者糖脂代谢的影响[J].中国康复医学杂志, 2017,32(8):907-911,937.
[12]郭彩红,高秀莹.达格列净对2型糖尿病伴代谢综合征患者代谢指标影响[J].临床军医杂志, 2018,46(5):585-587.
[13]沈亚非,徐焱成.高糖高脂膳食和链脲佐菌素诱导2型糖尿病模型的建立[J].实用诊断与治疗杂志, 2006,20(9):649-650.
[14]许冬明,李显国.高脂饮食及运动对2型糖尿病大鼠脂联素、瘦素及血糖水平的影响[J].中国应用生理学杂志, 2016,32(4):298-300.
[15]杨丽丽,董建成,卞尧尧,等.果糖胺检测对糖尿病诊断价值的Meta分析[J].中国糖尿病杂志, 2015,23(7):621-626.
[16]Roberts CK, Little JP, Thyfault JP. Modification of insulin sensitivity and glycemic control by activity and exercise. Med Sci Sports Exerc. 2013;45(10):1868-1877.
[17]邵月.耐力运动对糖尿病大鼠血清血糖、胰岛素、胰岛素抵抗指数、糖化血清蛋白、红细胞糖化血红蛋白的影响[J].中国老年学杂志, 2010,30(1):54-56.
[18]Khan S, Jena G. Sodium butyrate reduces insulin-resistance,fat accumulation and dyslipidemia in type-2 diabetic rat:A comparative study with metformin. Chem Biol Interact. 2016;254:124-134.
[19]Garabadu D, Krishnamurthy S. Metformin attenuates hepatic insulin resistance in type-2 diabetic rats through PI3K/Akt/GLUT-4 signalling independent to bicuculline-sensitive GABAA receptor stimulation. Pharm Biol. 2017;55(1):722-728.
[20]江洪.不同运动强度干预2型糖尿病模型大鼠的骨密度变化[J].中国组织工程研究, 2016,20(27):3963-3969.
[21]姜得悦,李婷,田晨光,等.二甲双胍抑制2型糖尿病大鼠脂肪组织胎球蛋白A表达的研究[J].中国糖尿病杂志, 2018,26(8):671-675.
[22]赵云鹏.中等强度有氧运动对2型糖尿病患者胰岛素抵抗相关因素的影响[J].广州体育学院学报, 2016,36(4):106-108.
[23]Jiménez-Maldonado A, Virgen-Ortiz A, Melnikov V, et al.Effect of moderate and high intensity chronic exercise on the pancreatic islet morphometry in healthy rats:BDNF receptor participation. Islets. 2017;9(1):1-10.