北京鸭关节炎病例的细菌分离与鉴定
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摘要
从表现为关节炎症状的北京鸭体内分离到23株细菌,通过16S rDNA测序鉴定,确定分离株分别属于葡萄球菌、棒状杆菌和大肠杆菌,另有一株球菌尚不明分类地位。药物敏感试验结果显示,阿莫西林、阿米卡星等对分离菌具有较好的抑菌作用,而氟苯尼考、阿奇霉素等抑菌效果较差。选取12个代表菌株进行动物试验,结果表明分离菌单独对肉鸭没有明显致病作用,说明这些细菌可能仅作为条件性致病菌存在于鸭场环境中。
23 bacterial strains were isolated from white Pekin ducks with arthritis symptoms. By 16 S rDNA sequencing, the isolated strainswere identified to belong to staphylococcus, corynebacteria and escherichia colirespectively, except one unclassified coccus. Drug sensitivity test showed that most of the bacterial strains were sensitive to amoxicillin, amikacin but resistant to florfenicol, azithromycin. 12 representative strains were tested by ducks challenge but no obviouspathogenic effect was found. It suggests that the tested strains maybe just opportunistic pathogens exist in the duck houses.
23 bacterial strains were isolated from white Pekin ducks with arthritis symptoms. By 16 S rDNA sequencing, the isolated strainswere identified to belong to staphylococcus, corynebacteria and escherichia colirespectively, except one unclassified coccus. Drug sensitivity test showed that most of the bacterial strains were sensitive to amoxicillin, amikacin but resistant to florfenicol, azithromycin. 12 representative strains were tested by ducks challenge but no obviouspathogenic effect was found. It suggests that the tested strains maybe just opportunistic pathogens exist in the duck houses.
引文
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[22] Mohan K., Shroeder-Tucker L. C., Karenga D.,Dziva F.,Harrison A.,Muvavarirwa P.Unidentified coryneform bacterial strain from cases of polyarthritis in chickens:phenotype and fatty acid profile[J].Avian diseases,2002,46(4):1051-1054.
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[2]王超,张梦南,乜琳,等.鹿场环境中细菌的分布及耐药性调查[J].中国畜牧兽医,2017(03):904-911.
[3]孙化露,陈冰,李树纯,等.7种鸭源细菌的分离与16S r DNA测序鉴定[J].中国兽医杂志,2011(10):13-16.
[4]陈世西,郭振华,杨汉春,等.猪源松鼠葡萄球菌的耐药性以及对消毒剂的抵抗力[A].中国畜牧兽医学会动物传染病学分会第十二次学术研讨会[C].江西,井冈山:2007.
[5]黎小正,韦信贤,童桂香,等.黄喉拟水龟致病菌——松鼠葡萄球菌的分离鉴定及药敏试验[J].大连水产学院学报,2009(04):371-374.
[6]段荟芹,王利.耗儿鱼松鼠葡萄球菌的分离鉴定及耐药性分析[J].中国畜牧兽医,2015(05):1288-1293.
[7]王贵花,申艳梅.松鼠葡萄球菌引起的脑室-腹腔分流术后感染1例分析[J].中国误诊学杂志,2011(06):1336.
[8]杨桂芝,段飞云.一株松鼠葡萄球菌引起的尿路感染[J].中国卫生检验杂志,2010(06):1568.
[9]赵红利,杨军,刘晓丽,等.鸭源葡萄球菌的分离鉴定和毒力基因检测及其与致病性相关性研究[J].中国兽医科学2018,2:1-5.
[10]杨军.鸭源葡萄球菌分离鉴定、毒力基因检测及与致病性相关性研究[D].武汉:华中农业大学,2014.
[11]洪帮兴,江丽芳,张世英,等.木糖葡萄球菌引发术后医院感染的鉴定与分析[J].中华医院感染学杂志,2002(11):74-75.
[12]路娟,仲淑英,董娟,等.由脓胸脓汁中分离出木糖葡萄球菌的鉴定[J].哈尔滨医科大学学报,1996(05):470-471.
[13]魏鲁予.致奶牛乳房炎木糖葡萄球菌部分生物学特性及Ⅰ类整合子检测[D].石河子:石河子大学,2010.
[14]侯大忠,麦充志,侯春阳.阿尔莱特葡萄球菌引起慢性前列腺炎1例报告[J].中国皮肤性病学杂志,2000(03):40.
[15]杨媛媛,岳启安,楚海荣,等.棒状杆菌条件致病菌临床感染及棒状杆菌的临床应用[J].医学综述,2007(17):1357-1359.
[16] Martin E., Kampfer P., Jackel U. Quantification and identification of culturable airborne bacteria from duck houses[J].The Annals of occupational hygiene, 2010,54(2):217-227.
[17]黄引贤,李乃津,赵国颂,等.羊伪结核棒状杆菌某些特性的初步研究[J].中国畜禽传染病,1988(02):3-4.
[18]李敏,田明星,邹年莉,等.一株猪源棒状杆菌的分离及其16S r RNA基因分析鉴定[J].中国畜牧兽医, 2010(10):76-81.
[19] Murphy J., Devane M. L., Robson B., Gilpin B.J. Genotypic characterization of bacteria cultured from duck faeces[J]. Journal of applied microbiology, 2005, 99(2):301-309.
[20] Xiao Y., Xiang Y., Zhou W., Chen J., Li K.,Yang H. Microbial community mapping in intestinal tract of broiler chicken[J]. Poultry science, 2017, 96(5):1387-1393.
[21] Yang W., Guo M., Liu G., Yu G., Wang P.,Wang H., Chai T. Detection and analysis of fine particulate matter and microbial aerosol in chicken houses in Shandong Province, China[J].Poultry science, 2017,97(3):995-1005.
[22] Mohan K., Shroeder-Tucker L. C., Karenga D.,Dziva F.,Harrison A.,Muvavarirwa P.Unidentified coryneform bacterial strain from cases of polyarthritis in chickens:phenotype and fatty acid profile[J].Avian diseases,2002,46(4):1051-1054.
[23] Glaeser S. P., Kleinhagauer T., Jackel U., Klug K.,Kampfer P.Jeotgalicoccus schoeneichii sp.nov.isolated from exhaust air of a pig barn[J].International journal of systematic and evolutionary microbiology,2016,66(9):3503-3508.
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