顶空固相微萃取与气质联用法分析贵州正安野木瓜果实中的挥发性成分
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摘要
实验以成熟正安野木瓜为原料,采用顶空固相微萃取与气质联用法,分析检测野木瓜果实中的挥发性成分。实验中分离鉴定出65种挥发性化合物,类别有酯类(23种)、醇类(21种)、醛类(8种)、醚类(3种)、羧酸类(3种)、酮类(4种)和烯烃类(2种)化合物,主要以酯类(35.906%)和醇类(33.589%)为主。实验结果表明,野木瓜果实的挥发性成分中正-3-己烯醇(Cis-3-hexen-1-ol)的含量最高为15.207%,具有强烈的青叶香气,是正安野木瓜果实清香气的主要成分。
The volatile aroma components of zhen'an Stauntonvine fruit pulp were separated and identified by headspace solid phase micro extraction couple with gas chromatography-mass spectrometry(HS-SPME-GC-MS). The results showed that a total of 65 volatile compounds including: 23 esters, 21 alcohols, 8 aldehydes, 3 ethers,3 carboxylic acids,4 ketones and 2 olefins. The esters were higher, accounting for 35.906% in the total volatile compounds, and the alcohols accounting for 33.598% in the second place. Besides, the Cis-3-hexen-1-ol was the highest volatile components of zhen'an Stauntonvine fruit, reached 15.207%, had a strong aroma of green leaves, which constituted the main aroma of zhen'an Stauntonvine fruit.
The volatile aroma components of zhen'an Stauntonvine fruit pulp were separated and identified by headspace solid phase micro extraction couple with gas chromatography-mass spectrometry(HS-SPME-GC-MS). The results showed that a total of 65 volatile compounds including: 23 esters, 21 alcohols, 8 aldehydes, 3 ethers,3 carboxylic acids,4 ketones and 2 olefins. The esters were higher, accounting for 35.906% in the total volatile compounds, and the alcohols accounting for 33.598% in the second place. Besides, the Cis-3-hexen-1-ol was the highest volatile components of zhen'an Stauntonvine fruit, reached 15.207%, had a strong aroma of green leaves, which constituted the main aroma of zhen'an Stauntonvine fruit.
引文
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[2]吴国卿,王文平,陈燕.野木瓜的资源状况与食用加工研究综述[J].贵州农业科学,2010,38(2):163-164.
[3]陈瑛,李锦,吴英良.野木瓜化学成分及其药理和临床研究进展[N].沈阳药科大学学报,2008,25(11):924-928.
[4]谢彦海,李晶,余良忠,等.野木瓜不同提取部位的镇痛抗炎作用研究[J].时珍国医国药,2015,26(4):861-862.
[5]祝小波,李宇婷,朱欣婷,等.野木瓜果实总皂苷体外抗肿瘤作用研究[J].安徽医药,2017,21(6):991-994.
[6]王文平.野木瓜多糖提取分离纯化及生物活性的研究[D].广州:华南理工大学,2008.
[7]邱顺华,金李芬,钱民章.正安野木瓜果实的抗菌活性成分分析[J].贵州农业科学,2013,41(5):54-57.
[8]崔霖芸.野木瓜提取液的抗氧化活性研究[J].中国酿造,2014,33(2):86-89.
[9]张秀璋,叶文博,周丽娜.野木瓜注射液对体外缺血清缺氧培养的小鼠脊髓神经细胞生长的影响[J].上海师范大学学报,2010,39(2):189-193.
[10]陈雪梅.野木瓜多糖对肝损伤大鼠的保护作用[J].中国老年学杂志,2016,36(4):775-777.