短小芽孢杆菌抗菌代谢物对斑马鱼的毒性研究
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摘要
试验研究了短小芽孢杆菌抗菌代谢物对斑马鱼96 h急性毒性并同时检测了乙酰胆碱酯酶、SOD、MDA、Na~+K~+-ATP酶等生化指标。结果表明:短小芽孢杆菌代谢物对斑马鱼的96 h LC_(50)为1.606 mg/l,95%置信区间为1.207～2.199 mg/l,代谢物浓度为1.5 mg/l以下时,短小芽孢杆菌抗菌代谢物对斑马鱼机体并没有造成损伤,但随着时间及浓度的增加,抗菌代谢物对斑马鱼的毒性作用开始明显,表现为MDA、Na~+K~+-ATP酶含量上升、SOD、乙酰胆碱酯酶含量下降,并有明显的时间效应、浓度效应关系。
In this experiment,the antibacterial metabolites of Bacillus pumilus were studied,and thebiochemical indexes such as Acetylcholinesterase,SOD,MDA and Na~+K~+-ATPase were detected.Theresults showed that the 96 h LC_(50 )of Bacillus pumilus metabolites to zebrafish was 1.606 mg/l,95%confidence interval was 1.207～2.199 mg/l.When the metabolite concentration was below 1.5 mg/l,theantibacterial metabolites of Bacillus pumilus had no damage to the organism,but with the increase ofthe time and concentration,the toxic effects of antimicrobial metabolites on zebrafish began to be obvi-ous,and it showing that MDA,Na~+K~+-ATPase content increased,SOD,Acetylcholinesterase content de-creased,and they had obvious time effect and concentration effect relationship.
In this experiment,the antibacterial metabolites of Bacillus pumilus were studied,and thebiochemical indexes such as Acetylcholinesterase,SOD,MDA and Na~+K~+-ATPase were detected.Theresults showed that the 96 h LC_(50 )of Bacillus pumilus metabolites to zebrafish was 1.606 mg/l,95%confidence interval was 1.207～2.199 mg/l.When the metabolite concentration was below 1.5 mg/l,theantibacterial metabolites of Bacillus pumilus had no damage to the organism,but with the increase ofthe time and concentration,the toxic effects of antimicrobial metabolites on zebrafish began to be obvi-ous,and it showing that MDA,Na~+K~+-ATPase content increased,SOD,Acetylcholinesterase content de-creased,and they had obvious time effect and concentration effect relationship.
引文
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[3]孔高飞.一种短小芽孢杆菌分离鉴定及培养条件研究[D].杭州:浙江理工大学,2014.
[4]刘国红.芽孢杆菌的系统进化及其分类学研究[J].福建农业学报,2008,23(4):436-339.
[5]于士强,桂俊鸿,王海燕.短小芽孢杆菌胞外蛋白质组双向电泳分析及碱性胁迫下胞外差异蛋白鉴定[J].应用与环境生物学报,2014,20(2):217-222.
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[8]孔志明.环境遗传毒理学[M].南京大学出版社,2009:172-174.
[9]吴迟,刘新刚,何明远,等.戊唑醇对斑马鱼的急性毒性及生物富集效应[J].生态毒理学报, 2017,12(4):302-309.
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[11]张年国,潘桂平,周文玉,等. 4种常见水产药物对菊黄东方鲀的急性毒性试验[J].水产科技情报,2015,42(6):339-342.
[12]刘海燕,张伟,薛敏,等.三聚氰胺对吉富罗非鱼的急性毒性研究[J].河北师范大学学报(自然科学版), 2009,33(5):659-664.
[13]李立.基于斑马鱼为指示生物的水体毒性试验研究[D].武汉:武汉理工大学, 2015.
[14] Lundqvist J, Hellman B, Oskarsson A. Fungicide Prochloraz Induces Oxidative Stress and DNA Damage in vitro[J]. Food and Chemical Toxicology, 2016, 9(1):36-41.
[15]臧晓霞,纪明山,左平春,等.啶酰菌胺对斑马鱼胚胎及成鱼的毒性[J].农药, 2016,55(12):918-921.
[16] Herbert N, Steffensen J. The response of atlantic cod,gadusmorhua,to progressive hypoxia:fish swimming speed and physiological stress[J]. Marine Biology,2015,147(6):1403-1412.
[17]杜娟娟.苯酚对斑马鱼行为学影响及毒理学效应研究[D].济南:山东建筑大学, 2016.
[2]杨佐忠.枯草芽孢杆菌PRS5抗生作用的初步研究[J].西南林学院学报, 1992,12(2):167-173.
[3]孔高飞.一种短小芽孢杆菌分离鉴定及培养条件研究[D].杭州:浙江理工大学,2014.
[4]刘国红.芽孢杆菌的系统进化及其分类学研究[J].福建农业学报,2008,23(4):436-339.
[5]于士强,桂俊鸿,王海燕.短小芽孢杆菌胞外蛋白质组双向电泳分析及碱性胁迫下胞外差异蛋白鉴定[J].应用与环境生物学报,2014,20(2):217-222.
[6] Sayeed I, Parvez S, Pandey S, et al. Oxidative stress biomarkers of exposure to deltamethrin in freshwater fish, channa punctatus bloch[J]. Ecotoxicologyand Environmental Safety, 2003,56(2):295-301.
[7]胡秀彩,杨小平,孙敬锋,等.敌敌畏对斑马鱼急性毒性试验[J].黑龙江水产, 2017(6):32-35.
[8]孔志明.环境遗传毒理学[M].南京大学出版社,2009:172-174.
[9]吴迟,刘新刚,何明远,等.戊唑醇对斑马鱼的急性毒性及生物富集效应[J].生态毒理学报, 2017,12(4):302-309.
[10]中华人民共和国国家环境保护部. GB/T 31270.12—2014化学农药环境安全评价试验准则:鱼类急性毒性试验[S].北京:中华人民共和国国家环境保护部, 2014.
[11]张年国,潘桂平,周文玉,等. 4种常见水产药物对菊黄东方鲀的急性毒性试验[J].水产科技情报,2015,42(6):339-342.
[12]刘海燕,张伟,薛敏,等.三聚氰胺对吉富罗非鱼的急性毒性研究[J].河北师范大学学报(自然科学版), 2009,33(5):659-664.
[13]李立.基于斑马鱼为指示生物的水体毒性试验研究[D].武汉:武汉理工大学, 2015.
[14] Lundqvist J, Hellman B, Oskarsson A. Fungicide Prochloraz Induces Oxidative Stress and DNA Damage in vitro[J]. Food and Chemical Toxicology, 2016, 9(1):36-41.
[15]臧晓霞,纪明山,左平春,等.啶酰菌胺对斑马鱼胚胎及成鱼的毒性[J].农药, 2016,55(12):918-921.
[16] Herbert N, Steffensen J. The response of atlantic cod,gadusmorhua,to progressive hypoxia:fish swimming speed and physiological stress[J]. Marine Biology,2015,147(6):1403-1412.
[17]杜娟娟.苯酚对斑马鱼行为学影响及毒理学效应研究[D].济南:山东建筑大学, 2016.