肥胖中医体质与单核苷酸多态性的关系研究
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摘要
目的探究肥胖人群中医体质的分布情况,进一步研究肥胖中医体质与肥胖FTO基因单核苷酸多态性的关系。方法收集健康人群/肥胖人群样本量分别为48、52例,测量体质量指数(BMI)、采外周血标本,采用改良多重高温连接酶检测反应技术(iMLDR)检测FTO基因上的3个SNP位点(rs9939609、rs1421085、rs8057044)。使用对其中的肥胖人群进行中医学体质分类。对健康人群和肥胖人群的FTO基因SNP位点进行分析,将健康人群/肥胖人群SNP突变结果、肥胖人群不同中医体质SNP突变结果进行统计学分析。结果 52例肥胖组人群中,按照中医体质由多到少依次为:肝郁气滞型、脾虚湿阻型、胃热湿阻型、脾肾两虚型、阴虚内热型,全身脂肪含量(FM)和体脂百分比上,5种体质差异无统计学意义(P>0.05)。在rs9939609中基因型及等位基因的概率经比较,差异无统计学意义(P>0.05);rs1421085中C基因及CC+CT基因型分布频率高于对照组,两组间差异有统计学意义(P<0.05);在rs8057044中A基因及AA+AG基因型分布频率高于对照组,两组间差异有统计学意义(P<0.05)。rs1421085的CT与CC基因型在脾虚湿阻型中大量分布,概率分别为61.53%、23.08%。rs8057044的AG与AA基因在脾肾两虚型中大量分布,概率分别为:50%、50%。rs8057044的AG与AA基因阴虚内热型中大量分布,概率分别为75%、25%。结论 FTO基因的rs9939609的A基因在肥胖组和对照组中未见明显差异,且不推荐作为中医体质分型的指标,rs1421085与脾虚湿阻型高度相关,rs8057044与脾肾两虚型和阴虚内热型高度相关。
Objective To explore the distribution of traditional chinese medicine(TCM)constitution symptoms in obese people,and further study the relationship between TCM constitution and FTO single nucleotide polymorphism of obesity gene.Methods 48 healthy people and 52 obese people were enrolled.Body mass index(BMI)was measured and peripheral blood samples were collected.Three SNP loci(rs9939609,rs1421085,rs8057044)in the FTO gene were detected by improved multiple hyperthermic ligase assay(iMLDR).The TCM constitution classification of obese people was carried out.The SNP loci of FTO gene in healthy and obese people were analyzed.The results of SNP mutation in healthy and obese people and in obese people with different TCM constitutions were statistically analyzed.Results Among 52 obese people,according to the number of constitutional people in TCM,the order from the large to small was liver-qi stagnation type,spleen-deficiency-dampness type,stomach-heat-dampness type,spleen-kidney deficiency type and yin-deficiency-internal-heat type.There was no significant difference in body fat percentage FM and body fat percentage between the five TCM constitutions(P>0.05).The probability of genotype and allele in rs9939609 had no statistical significance(P>0.05),and the frequency of C gene and CC+CT genotype in rs1421085 was higher than that in the control group,and there was statistical significance between the two groups(P<0.05).The frequency of A gene and AA+AG genotype in rs8057044 was higher than that in the control group,and there was statistical difference between the two groups(P<0.05).The CT and CC genotypes of rs1421085 were largely distributed in spleen-deficiency-dampness type,with a probability of 61.53% and23.08%respectively.The AG and AA genes of rs8057044 were largely distributed in spleen-kidney deficiency type,with a probability of 50%and 50%respectively.The AG and AA genes of rs8057044 were largely distributed in yin-deficiency-internal-heat type,with a probability of 75%and 25%respectively.Conclusion The rs9939609 of A gene of FTO gene was not significantly different between the obese group and the control group,and was not recommended as an indicator of TCM constitution type.rs1421085 was highly correlated with spleen deficiency dampness type.rs8057044 was highly correlated with spleen-kidney deficiency type and yin-deficiency-internal-heat type.
Objective To explore the distribution of traditional chinese medicine(TCM)constitution symptoms in obese people,and further study the relationship between TCM constitution and FTO single nucleotide polymorphism of obesity gene.Methods 48 healthy people and 52 obese people were enrolled.Body mass index(BMI)was measured and peripheral blood samples were collected.Three SNP loci(rs9939609,rs1421085,rs8057044)in the FTO gene were detected by improved multiple hyperthermic ligase assay(iMLDR).The TCM constitution classification of obese people was carried out.The SNP loci of FTO gene in healthy and obese people were analyzed.The results of SNP mutation in healthy and obese people and in obese people with different TCM constitutions were statistically analyzed.Results Among 52 obese people,according to the number of constitutional people in TCM,the order from the large to small was liver-qi stagnation type,spleen-deficiency-dampness type,stomach-heat-dampness type,spleen-kidney deficiency type and yin-deficiency-internal-heat type.There was no significant difference in body fat percentage FM and body fat percentage between the five TCM constitutions(P>0.05).The probability of genotype and allele in rs9939609 had no statistical significance(P>0.05),and the frequency of C gene and CC+CT genotype in rs1421085 was higher than that in the control group,and there was statistical significance between the two groups(P<0.05).The frequency of A gene and AA+AG genotype in rs8057044 was higher than that in the control group,and there was statistical difference between the two groups(P<0.05).The CT and CC genotypes of rs1421085 were largely distributed in spleen-deficiency-dampness type,with a probability of 61.53% and23.08%respectively.The AG and AA genes of rs8057044 were largely distributed in spleen-kidney deficiency type,with a probability of 50%and 50%respectively.The AG and AA genes of rs8057044 were largely distributed in yin-deficiency-internal-heat type,with a probability of 75%and 25%respectively.Conclusion The rs9939609 of A gene of FTO gene was not significantly different between the obese group and the control group,and was not recommended as an indicator of TCM constitution type.rs1421085 was highly correlated with spleen deficiency dampness type.rs8057044 was highly correlated with spleen-kidney deficiency type and yin-deficiency-internal-heat type.
引文
[1]刘丹.代谢正常肥胖转归糖尿病风险的队列研究及FTO SNP rs9939609与糖尿病关系的研究[D].西南医科大学,2017.
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[10]SANG G K,LEE J S,BAE H H,et al.Distinguishing the Korean silage corn varieties through development of PCRbased SNP marker[J].2017,37(2):168-175.
[11]李文君,朱可,赵冬雪,等.Fetuin-A基因多态性(rs4917,rs4918)与动脉粥样硬化性脑梗死的关系[J].中风与神经疾病杂志,2016,3(8):719-723.
[12]李思勤,邹秉杰,王建平,等.焦磷酸测序测定SNP的常见问题与解决方法[J].现代生物医学进展,2014,14(12):2219-2223.
[13]赵丽荣.PPARG2基因Pro12Ala多态与呼市地区汉族人群心肌梗死和肥胖的关系[D].呼和浩特:内蒙古医科大学,2010.
[14] HOSSEINI-ESFAHANI F,KOOCHAKPOOR G,DANESHPOUR M S,et al.Mediterranean dietary pattern adherence modify the association between FTO genetic variations and obesity phenotypes[J].Nutrients,2017,9(10):1064.
[15]朱丽冰,王济,李玲孺,等.超重和肥胖人群的中医兼夹体质分析[J].中华中医药学刊,2017(1):161-165.
[16]芦波,周训杰,符德玉,等.腹型肥胖高血压中医证候特点及与靶器官损害的相关性分析[J].中西医结合心脑血管病杂志,2017,15(1):1-5.
[17]ELOUEJ S,BELFKIBENALI H,NAGARA M,et al.Association of rs9939609polymorphism with metabolic parameters and FTO risk haplotype among tunisian metabolic syndrome[J].Metab Syndr Relat Dis,2016,14(2):121.
[2] GONZLEZSNCHEZ J L,ZABENA C,MARTNEZLA RRAD M T,et al.Variant rs9939609in the FTO gene is associated with obesity in an adult population from Spain.[J].Clinical Endocrinology,2010,70(3):390-393.
[3]王亚男,董学君,徐秀平,等.FTO多态性与乳腺癌易感性的荟萃分析[J/CD].中华临床实验室管理电子杂志,2017,5(3):165-170.
[4]王琦,朱燕波,吴玉娥,等.肥胖,超重与正常体质量高血压患者的中医体质类型分布特点比较研究[J].中华中医药杂志,2011,26(8):1676-1679.
[5]张笑梅,朱燕波,邬宁茜,等.基于不同指标诊断的肥胖与中医体质的相关性分析[J].中医杂志,2015,56(3):212-215.
[6]胡畅.基于iMLDR技术的耳聋基因突变热点的新型快速检测方法研究[D].长沙:中南大学,2013.
[7] MAJDI M A,MOHAMMADZADEH N A,LOTFI H,et al.Correlation of resistin serum level with fat mass and obesity-associated gene(FTO)rs9939609polymorphism in obese women with type 2diabetes[J].Diabetes&Metabolic Syndrome,2017,11(Suppl 2):S715.
[8]孙升云,王凤珍.中医药防治单纯性肥胖的临床研究进展[J].时珍国医国药,2006,17(3):331-332.
[9]王瑞恒,刘利民,赵金玲,等.基于等位基因特异性PCR原理建立的SNP分型新方法[J].法医学杂志,2008,24(3):189-193.
[10]SANG G K,LEE J S,BAE H H,et al.Distinguishing the Korean silage corn varieties through development of PCRbased SNP marker[J].2017,37(2):168-175.
[11]李文君,朱可,赵冬雪,等.Fetuin-A基因多态性(rs4917,rs4918)与动脉粥样硬化性脑梗死的关系[J].中风与神经疾病杂志,2016,3(8):719-723.
[12]李思勤,邹秉杰,王建平,等.焦磷酸测序测定SNP的常见问题与解决方法[J].现代生物医学进展,2014,14(12):2219-2223.
[13]赵丽荣.PPARG2基因Pro12Ala多态与呼市地区汉族人群心肌梗死和肥胖的关系[D].呼和浩特:内蒙古医科大学,2010.
[14] HOSSEINI-ESFAHANI F,KOOCHAKPOOR G,DANESHPOUR M S,et al.Mediterranean dietary pattern adherence modify the association between FTO genetic variations and obesity phenotypes[J].Nutrients,2017,9(10):1064.
[15]朱丽冰,王济,李玲孺,等.超重和肥胖人群的中医兼夹体质分析[J].中华中医药学刊,2017(1):161-165.
[16]芦波,周训杰,符德玉,等.腹型肥胖高血压中医证候特点及与靶器官损害的相关性分析[J].中西医结合心脑血管病杂志,2017,15(1):1-5.
[17]ELOUEJ S,BELFKIBENALI H,NAGARA M,et al.Association of rs9939609polymorphism with metabolic parameters and FTO risk haplotype among tunisian metabolic syndrome[J].Metab Syndr Relat Dis,2016,14(2):121.