术前跑台训练在改善术后大鼠认知功能中的应用效果
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摘要
目的观察术前跑台训练改善术后大鼠认知功能的作用。方法将40只SPF级健康的老年SD雄性大鼠随机分为对照组和治疗组,各20只。治疗组大鼠术前2周进行电动跑台训练,30 min/d。对照组大鼠仅置于跑台机上30 min/d,不进行训练。训练结束1周后对两组大鼠均进行脾切除术。术后2周,所有大鼠均行Morris水迷宫实验,评估大鼠认知功能的变化,并测定大鼠海马区ChAT和AchE的活性。结果定位航行实验中,随着训练次数增加,两组大鼠逃避潜伏期时间均有所缩短;定位航行训练第1～5天,治疗组大鼠逃避潜伏期时间均明显短于对照组(P<0.05)。在空间探索实验中,治疗组大鼠在目标象限区内游泳时间占总游泳时间的百分比高于对照组,且60 s内跨越原平台次数明显多于对照组(P<0.05)。治疗组大鼠海马区ChAT活性高于对照组大鼠,而AchE活性低于对照组大鼠(P<0.05)。结论术前跑台训练可改善术后大鼠的认知功能,其作用机制可能为上调大鼠海马区ChAT活性并下调AchE活性。
Objective To observe the role of preoperative treadmill training in the improvement of postoperative rats' cognitive function. Methods Forty healthy old SD male rats of SPF grade were randomly divided into control group and treatment group, with 20 rats in each group. The rats in the treatment group received electric treadmill training from 2 weeks before operation, with 30 min/d. The rats in the control group were only placed on the treadmill for 30 min/d without training.Splenectomy was performed in both groups at one week after training. Morris water maze test was performed in all rats at2 weeks after operation to evaluate the changes of cognitive function and the activities of ChAT and AchE in hippocampus of all rats were determined. Results In the positioning navigation experiment, with the increase of training times, the time of escape latency of rats in both groups shortened, and the time of escape latency of rats in the treatment group were significantly shorter than those in the control group from the first to fifth days of the training(P <0.05). In the space exploration experiment, the percentage of swimming time in the target quadrant area to total swimming time in the treatment group was higher than that in the control group, and the number of crossing the original platform in 60 s was significantly more than that in the control group(P<0.05). The ChAT activity in the hippocampus of the treatment group was higher than that of the control group, while the AchE activity was lower than that of the control group(P <0.05). Conclusion The preoperative treadmill training is capable of improving the postoperative rats' cognitive function, its mechanism may be to up-regulating the activity of ChAT and down-regulating the activity of AchE in rats' hippocampus.
Objective To observe the role of preoperative treadmill training in the improvement of postoperative rats' cognitive function. Methods Forty healthy old SD male rats of SPF grade were randomly divided into control group and treatment group, with 20 rats in each group. The rats in the treatment group received electric treadmill training from 2 weeks before operation, with 30 min/d. The rats in the control group were only placed on the treadmill for 30 min/d without training.Splenectomy was performed in both groups at one week after training. Morris water maze test was performed in all rats at2 weeks after operation to evaluate the changes of cognitive function and the activities of ChAT and AchE in hippocampus of all rats were determined. Results In the positioning navigation experiment, with the increase of training times, the time of escape latency of rats in both groups shortened, and the time of escape latency of rats in the treatment group were significantly shorter than those in the control group from the first to fifth days of the training(P <0.05). In the space exploration experiment, the percentage of swimming time in the target quadrant area to total swimming time in the treatment group was higher than that in the control group, and the number of crossing the original platform in 60 s was significantly more than that in the control group(P<0.05). The ChAT activity in the hippocampus of the treatment group was higher than that of the control group, while the AchE activity was lower than that of the control group(P <0.05). Conclusion The preoperative treadmill training is capable of improving the postoperative rats' cognitive function, its mechanism may be to up-regulating the activity of ChAT and down-regulating the activity of AchE in rats' hippocampus.
引文
[1] HU Z,OU Y,DUAN K,et al.Inflammation:a bridge between postoperative cognitive dysfunction and Alzheimer's disease[J].Med Hypotheses,2010,74(4):722-724.
[2]肖建波.右美托咪定对全麻骨科手术老年患者术后认知功能的影响[J].临床医学研究与实践,2017,2(25):61-62.
[3]魏小利,袁媛,徐亚红.认知康复训练对缺血性脑卒中患者认知功能障碍的影响[J].临床医学研究与实践,2016,1(22):149-151.
[4]王妍,王莹.术前认知功能训练对全麻老年患者术后早期认知功能障碍的影响[J].哈尔滨医科大学学报,2017,51(2):183-185.
[5] KIM DH,KO IG,KIM BK,et a1.Treadmill exercise inhibits traumatic brain injury-induced hippocampal apoptosis[J].Physiol Behav,2010,101(5):660-665.
[6]陈小璐,蒋莉.运动康复对缺氧缺血性脑损伤新生大鼠空间学习记忆的影响[J].中国当代儿科杂志,2010,12(5):363-367.
[7]许敬,钟卫权,杨波,等.运动后大鼠小脑乙酰胆碱酯酶的动态变化及其对运动调节的作用[J].中国组织工程研究,2004,8(9):1736-1737.
[8] ZHANG HY,ZHENG CY,YAN H,et al.Potential therapeutic targets of huperzine A for Alzheimer's disease and vascular dementia[J].Chem Biol Interact,2008,175(1-3):396-402.
[9]张泰鹏,莫雪安,刘金萍,等.甘露醇对骨髓间充质干细胞治疗血管性痴呆大鼠行为学及海马胆碱能系统的影响[J].中风与神经疾病,2011,28(4):317-320.
[2]肖建波.右美托咪定对全麻骨科手术老年患者术后认知功能的影响[J].临床医学研究与实践,2017,2(25):61-62.
[3]魏小利,袁媛,徐亚红.认知康复训练对缺血性脑卒中患者认知功能障碍的影响[J].临床医学研究与实践,2016,1(22):149-151.
[4]王妍,王莹.术前认知功能训练对全麻老年患者术后早期认知功能障碍的影响[J].哈尔滨医科大学学报,2017,51(2):183-185.
[5] KIM DH,KO IG,KIM BK,et a1.Treadmill exercise inhibits traumatic brain injury-induced hippocampal apoptosis[J].Physiol Behav,2010,101(5):660-665.
[6]陈小璐,蒋莉.运动康复对缺氧缺血性脑损伤新生大鼠空间学习记忆的影响[J].中国当代儿科杂志,2010,12(5):363-367.
[7]许敬,钟卫权,杨波,等.运动后大鼠小脑乙酰胆碱酯酶的动态变化及其对运动调节的作用[J].中国组织工程研究,2004,8(9):1736-1737.
[8] ZHANG HY,ZHENG CY,YAN H,et al.Potential therapeutic targets of huperzine A for Alzheimer's disease and vascular dementia[J].Chem Biol Interact,2008,175(1-3):396-402.
[9]张泰鹏,莫雪安,刘金萍,等.甘露醇对骨髓间充质干细胞治疗血管性痴呆大鼠行为学及海马胆碱能系统的影响[J].中风与神经疾病,2011,28(4):317-320.