青霉素喷施防治柑橘黄龙病试验初报
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摘要
柑橘黄龙病是世界性分布的柑橘毁灭性病害,严重制约着柑橘产业的发展.为探究青霉素对柑橘黄龙病的防治效果,本试验采用喷施的方式对柑橘黄龙病病树进行处理,同时结合实时荧光定量PCR技术对病树内黄龙病菌量进行动态监测,确定青霉素对柑橘黄龙病的相对防效.结果表明连续喷施4次青霉素3个月和7个月后病树中黄龙病菌含量均出现不同程度的上调,相对防效分别为-170.16%和-63.96%.以上结果表明喷施的方式进行青霉素处理黄龙病病树对柑橘黄龙病并不能取到防治效果.
Citrus Huanglongbing(HLB), the most devastating citrus disease worldwide, restricts the development of the citrus industry severely. In order to investigate the control effect of penicillin on Citrus HLB, the test was conducted by means of spraying to treat diseased trees which were infected by bacterial pathogen Candidatus Liberibacter asiaticus(Las). The real-time fluorescence quantitative PCR technique was used to monitor the amount of Huanglongbing pathogen in diseased trees dynamically and determine the relative control effect of penicillin on Las. The results showed that the content of Las in diseased trees at the 3 rd and 7 th months after continuous spraying of penicillin 4 times was up-regulated. At the same time, the relative control effects of the penicillin solution were-170.16% and-63.96%, respectively. The above results indicate that the penicillin treatment has no effect on HLB.
Citrus Huanglongbing(HLB), the most devastating citrus disease worldwide, restricts the development of the citrus industry severely. In order to investigate the control effect of penicillin on Citrus HLB, the test was conducted by means of spraying to treat diseased trees which were infected by bacterial pathogen Candidatus Liberibacter asiaticus(Las). The real-time fluorescence quantitative PCR technique was used to monitor the amount of Huanglongbing pathogen in diseased trees dynamically and determine the relative control effect of penicillin on Las. The results showed that the content of Las in diseased trees at the 3 rd and 7 th months after continuous spraying of penicillin 4 times was up-regulated. At the same time, the relative control effects of the penicillin solution were-170.16% and-63.96%, respectively. The above results indicate that the penicillin treatment has no effect on HLB.
引文
[1]程春振, 曾继吾, 钟云, 等. 柑橘黄龙病研究进展[J]. 园艺学报, 2013, 40(9):1656-1668.
[2]REINKING O A. Diseases of economic plants in southern China[J]. Philippine Agricultural, 1919, 8: 109-135.
[3]余继华, 汪恩国, 杨晓, 等. 柑橘黄龙病老龄果园发病力与时序发生规律研究[J]. 农学学报, 2017, 7(4): 10-14.
[4]许美容, 戴泽翰, 孔维文, 等. 基于分子技术的柑橘黄龙病研究进展[J]. 果树学报, 2015, 32(2):322-334.
[5]陈文利, 徐婉, 程保平, 等. 柑橘黄龙病检测及治疗方法的研究进展[J]. 华南师范大学学报 (自然科学版), 2017, 49(5):9-15.
[6]余继华, 黄振东, 张敏荣, 等. 亚洲柑橘木虱带菌率的周年变化动态[J]. 浙江大学学报 (农业与生命科学版), 2017, 43(1):89-94.
[7]LIN K H. Etiological studies of yellow shoot of citrus[J]. Acta Phytopathologica Sinica, 1956, 2: 13-42.
[8]贾志成, 郑加强, 黄雅杰, 等. 柑橘黄龙病热处理防治技术研究进展[J]. 农业工程学报,2015, 31(23):1-9.
[9]范国成, 林雄杰, 王贤达, 等. 田间自然热罩治疗柑橘黄龙病的效果分析[J]. 果树学报, 2016, 33(9): 1139-1147.
[10]陈仕钦, 卢小林, 陈玉龙, 等. 柑橘黄龙病防控药剂筛选试验初报[J]. 植物保护, 2014, 40(2):166-170.
[11]ZHANG M, POWELL C A, BENYON L S, et al. Deciphering the bacterial microbiome of citrus plants in response to ‘Candidatus Liberibacter asiaticus’-infection and antibiotic treatments[J]. PLoS One, 2013, 8(11): e76331.
[12]ZHANG M, GUO Y, POWELL C A, et al. Effective antibiotics against ‘Candidatus Liberibacter asiaticus’ in HLB-affected citrus plants identified via the graft-based evaluation[J]. PLoS One. 2014, 9(11): e111032.
[13]ZOU X, JIANG X, XU L, et al. Transgenic citrus expressing synthesized cecropin B genes in the phloem exhibits decreased susceptibility to Huanglongbing[J]. Plant Molecular Biology, 2017, 93(4-5):341-353.
[14]ZHANG M, POWELL C A, ZHOU L, et al. Chemical compounds effective against the Citrus Huanglongbing bacterium ‘Candidatus Liberibacter asiaticus’ in planta[J]. Phytopathology, 2011, 101(9): 1097-1013.
[15]MCMANUS P S, STOCKWELL V O, SUNDIN G W, et al. Antibiotic use in plant agriculture[J]. Annual Review of Phytopathology, 2002, 40:443-465.
[16]ZHANG M, POWELL C A, GUO Y, et al. A graft-based chemotherapy method for screening effective molecules and rescuing huanglongbing-affected citrus plants[J]. Phytopathology, 2002, 102: 567-574.
[17]ZHANG M, DUAN Y, ZHOU L, et al. Screening molecules for control of citrus huanglongbing using an optimized regeneration system for ‘Candidatus Liberibacter asiaticus’-infected periwinkle (Catharanthus roseus) cuttings[J]. Phytopathology, 2010, 100: 239-245.
[18]MCCOY R E. Use of tetracycline antibiotics to control yellows diseases[J]. Plant Disease, 1982, 66: 539-542.
[19]孟华岳, 文英杰, 徐汉虹. 亚洲柑橘木虱和柑橘黄龙病的化学防治[J]. 世界农药, 2016, 38(1): 21-31.
[2]REINKING O A. Diseases of economic plants in southern China[J]. Philippine Agricultural, 1919, 8: 109-135.
[3]余继华, 汪恩国, 杨晓, 等. 柑橘黄龙病老龄果园发病力与时序发生规律研究[J]. 农学学报, 2017, 7(4): 10-14.
[4]许美容, 戴泽翰, 孔维文, 等. 基于分子技术的柑橘黄龙病研究进展[J]. 果树学报, 2015, 32(2):322-334.
[5]陈文利, 徐婉, 程保平, 等. 柑橘黄龙病检测及治疗方法的研究进展[J]. 华南师范大学学报 (自然科学版), 2017, 49(5):9-15.
[6]余继华, 黄振东, 张敏荣, 等. 亚洲柑橘木虱带菌率的周年变化动态[J]. 浙江大学学报 (农业与生命科学版), 2017, 43(1):89-94.
[7]LIN K H. Etiological studies of yellow shoot of citrus[J]. Acta Phytopathologica Sinica, 1956, 2: 13-42.
[8]贾志成, 郑加强, 黄雅杰, 等. 柑橘黄龙病热处理防治技术研究进展[J]. 农业工程学报,2015, 31(23):1-9.
[9]范国成, 林雄杰, 王贤达, 等. 田间自然热罩治疗柑橘黄龙病的效果分析[J]. 果树学报, 2016, 33(9): 1139-1147.
[10]陈仕钦, 卢小林, 陈玉龙, 等. 柑橘黄龙病防控药剂筛选试验初报[J]. 植物保护, 2014, 40(2):166-170.
[11]ZHANG M, POWELL C A, BENYON L S, et al. Deciphering the bacterial microbiome of citrus plants in response to ‘Candidatus Liberibacter asiaticus’-infection and antibiotic treatments[J]. PLoS One, 2013, 8(11): e76331.
[12]ZHANG M, GUO Y, POWELL C A, et al. Effective antibiotics against ‘Candidatus Liberibacter asiaticus’ in HLB-affected citrus plants identified via the graft-based evaluation[J]. PLoS One. 2014, 9(11): e111032.
[13]ZOU X, JIANG X, XU L, et al. Transgenic citrus expressing synthesized cecropin B genes in the phloem exhibits decreased susceptibility to Huanglongbing[J]. Plant Molecular Biology, 2017, 93(4-5):341-353.
[14]ZHANG M, POWELL C A, ZHOU L, et al. Chemical compounds effective against the Citrus Huanglongbing bacterium ‘Candidatus Liberibacter asiaticus’ in planta[J]. Phytopathology, 2011, 101(9): 1097-1013.
[15]MCMANUS P S, STOCKWELL V O, SUNDIN G W, et al. Antibiotic use in plant agriculture[J]. Annual Review of Phytopathology, 2002, 40:443-465.
[16]ZHANG M, POWELL C A, GUO Y, et al. A graft-based chemotherapy method for screening effective molecules and rescuing huanglongbing-affected citrus plants[J]. Phytopathology, 2002, 102: 567-574.
[17]ZHANG M, DUAN Y, ZHOU L, et al. Screening molecules for control of citrus huanglongbing using an optimized regeneration system for ‘Candidatus Liberibacter asiaticus’-infected periwinkle (Catharanthus roseus) cuttings[J]. Phytopathology, 2010, 100: 239-245.
[18]MCCOY R E. Use of tetracycline antibiotics to control yellows diseases[J]. Plant Disease, 1982, 66: 539-542.
[19]孟华岳, 文英杰, 徐汉虹. 亚洲柑橘木虱和柑橘黄龙病的化学防治[J]. 世界农药, 2016, 38(1): 21-31.