金花葵花黄酮提取物不同溶剂萃取物的抗氧化活性
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摘要
目的:研究金花葵花黄酮不同极性溶剂萃取物的体外抗氧化能力,并考察黄酮含量与抗氧化活性的相关性。方法:采用不同极性溶剂对金花葵花乙醇提取物进行液-液萃取,得到金花葵花黄酮样品为:石油醚相、乙酸乙酯相、正丁醇相、水相萃取物。测定不同极性溶剂萃取物中总黄酮含量,研究各萃取物的清除DPPH自由基能力、清除羟基自由基能力、还原能力、清除超氧阴离子能力和抗油脂氧化能力,分析黄酮含量与抗氧化活性的相关关系。结果:金花葵花黄酮不同极性溶剂萃取物均有抗氧化活性,顺序均为乙酸乙酯相>正丁醇相>乙醇提取物>石油醚相>水相。在0.20～1.00 mg/mL的萃取物质量浓度范围内,随着质量浓度的增加,抗氧化活性相应增大。质量浓度在1.00 mg/mL时,乙酸乙酯和正丁醇相萃取物的抗氧化活性接近于V_C。各萃取物中黄酮含量与抗氧化活性呈现较好相关关系(p<0.05)。结论:金花葵花黄酮具有较高的体外抗氧化能力,乙酸乙酯相和正丁醇相萃取物可作为分离活性成分的研究重点。
Objective:To study the in vitro antioxidant capacity of different polar solvent extracts of flavonoids from Aurea helianthus flower,and to investigate the correlation between flavonoids content and antioxidant activity. Methods:The ethanol extracts of Aurea helianthus flower were extracted by liquid-liquid extraction with different polar solvents. The flavonoids from Aurea helianthus flower were obtained:petroleum ether phase,ethyl acetate phase,n-butanol phase and aqueous phase extract. The total flavonoids in different polar solvent extracts were determined. The DPPH free radical scavenging ability,hydroxyl radical scavenging ability,reducing ability,superoxide anion ability and antioxidant capacity of the extracts were studied. The relationship between antioxidant activity and flavonoid content were analyzed. Results:The results showed that solvent extracts of flavonoids with different polarities from Aurea helianthus flower had antioxidant activities,and the antioxidant activities of ethyl acetate phase was the strongest,followed by n-butanol phase,ethanol extract,petroleum ether phase,aqueous phase. In the range of the mass concentration of the extract from 0.20 to 1.00 mg/mL,the antioxidant activity increased correspondingly with the increase of the mass concentration. At the mass concentration of extract was 1.00 mg/mL,the antioxidant activity of the ethyl acetate and n-butanol phase extracts was close to V_C. There was a good correlation between flavonoid content and antioxidant activity in each extract(p<0.05).Conclusion:The flavonoids of Aurea helianthus flower have high antioxidant capacity in vitro,and the ethyl acetate phase and n-butanol phase extract can be used as the research focus of separating active ingredients.
Objective:To study the in vitro antioxidant capacity of different polar solvent extracts of flavonoids from Aurea helianthus flower,and to investigate the correlation between flavonoids content and antioxidant activity. Methods:The ethanol extracts of Aurea helianthus flower were extracted by liquid-liquid extraction with different polar solvents. The flavonoids from Aurea helianthus flower were obtained:petroleum ether phase,ethyl acetate phase,n-butanol phase and aqueous phase extract. The total flavonoids in different polar solvent extracts were determined. The DPPH free radical scavenging ability,hydroxyl radical scavenging ability,reducing ability,superoxide anion ability and antioxidant capacity of the extracts were studied. The relationship between antioxidant activity and flavonoid content were analyzed. Results:The results showed that solvent extracts of flavonoids with different polarities from Aurea helianthus flower had antioxidant activities,and the antioxidant activities of ethyl acetate phase was the strongest,followed by n-butanol phase,ethanol extract,petroleum ether phase,aqueous phase. In the range of the mass concentration of the extract from 0.20 to 1.00 mg/mL,the antioxidant activity increased correspondingly with the increase of the mass concentration. At the mass concentration of extract was 1.00 mg/mL,the antioxidant activity of the ethyl acetate and n-butanol phase extracts was close to V_C. There was a good correlation between flavonoid content and antioxidant activity in each extract(p<0.05).Conclusion:The flavonoids of Aurea helianthus flower have high antioxidant capacity in vitro,and the ethyl acetate phase and n-butanol phase extract can be used as the research focus of separating active ingredients.
引文
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[21]杨琼琼,刘雄民,赖芳,等.柿木皮单宁不同极性组分对油脂抗氧化作用研究[J].中国油脂,2016,41(5):45-49.
[22]徐连巧,曹剑锋,谢之英,等.响应面法优化小春花根黄酮提取工艺及其抗油脂氧化活性研究[J].食品安全质量检测学报,2018,9(5):985-992.
[23]王晓静,陈莉华,黄玉龙,等.火棘果黄酮对油脂的抗氧化活性[J].天然产物研究与开发,2015,27:909-914.
[24]关海宁,刁小琴,乔秀丽,等.提取方式对玉米须总多酚抗氧化活性的影响研究[J].食品工业,2018,39(8):25-29.
[25]张锦华,徐蔓,白宝清,等.响应面法优化提取无花果干果中多酚和总黄酮物质及其抗氧化活性[J].食品工业科技,2018,39(16):183-190.
[2]曹利华,苗明三.金花葵的现代研究与综合利用分析[J].中医学报,2016,31(12):1966-1968.
[3]刘剑,罗仓学,伍贤密,等.响应面法优化金花葵籽多糖提取工艺研究[J].食品研究与开发,2018,39(1):25-29.
[4]巫玲丽,穆祯强,张利,等.金花葵多糖提取的工艺优化及抗氧化活性研究[J].南方农业学报,2017,48(1):109-113.
[5]陈冯钱,黄杰涛,郑婷婷,等.微波辅助提取金花葵多糖工艺及体外抗氧化性研究[J].食品研究与开发,2017,38(19):47-50.
[6]刘琦,辛秀兰,兰蓉,等.金花葵花中总黄酮提取工艺的研究[J].食品研究与开发,2011,32(7):19-21.
[7]宋琳琳,黄蓓蓓,张朝晖.金花葵花中总黄酮的超声波提取工艺[J].贵州农业科学,2016,44(2):70-72.
[8]张路,于慧敏,杜凤沛,等.金花葵中总黄酮的分离提取及含量测定[J].吉林师范大学学报,2015(4):104-107.
[9]陈亮,辛秀兰,苏东海,等.金花葵花总黄酮的精制工艺研究[J].江苏农业科学,2016,44(8):339-343.
[10]申利英,高玉红,任立伟,等.响应曲面法优化金花葵总黄酮超声提取工艺[J].煤炭与化工,2017,40(9):24-31.
[11]云成悦,李潇彬,郑奎玲,等.头花蓼多酚不同极性溶剂萃取物的抗氧化活性[J].食品工业科技,2018,39(3):61-64.
[12]Viacava G E,Roura S I.Principal component and hierarchical cluster analysis to select natural elicitors for enhancing phytochemical content and antioxidant activity of lettuce sprouts[J].Scientia Horticulturae,2015,193:13-21.
[13]Priyanka C,Kadam D A,Kadam A S,et al.Free radical scavenging(DPPH)and ferric reducing ability(FRAP)of some gymnosperm species[J].Ijbpas Com,2013,3(2):34-36.
[14]Chen R Z.Antioxidant and immunobiological activity of water-soluble polysaccharide fractions purified from Acanthopanax senticosu[J].Food Chemistry,2011,127(2):434-440.
[15]YIldirim A,Oktay M,Bilaloglu V.The antioxidant activity of the leaves of Cydonia vulgaris[J].Turkish Journal of Medical Sciences,2001,31(1):23-27.
[16]Liang D,Zhou Q,Gong W,et al.Studies on the antioxidant and hepatoprotective activities of polysaccharides from Talinum triangulare[J].J Ethnopharmacol,2011,136(2):316-321.
[17]刘丹丹,李炳奇,陈韩英,等.沙枣不同果、叶中多酚类物质抗氧化能力比较的研究[J].食品工业科技,2011,32(12):93-95.
[18]刘继攀,张紫华,关振华,等.两种真姬菇不同溶剂提取物的抗氧化活性研究[J].食品工业科技,2018,39(11):56-61.
[19]罗磊,张冰洁,马丽苹,等.金银花叶黄酮体外抗氧化能力及对H2O2诱导RAW264.7巨噬细胞损伤的保护作用[J].食品科学,2018,39(11):139-145.
[20]甘芝霖,倪元颖,郭悦,等.大孔树脂分离纯化玫瑰果多酚及其抗氧化性[J].农业工程学报,2015,31(24):298-306.
[21]杨琼琼,刘雄民,赖芳,等.柿木皮单宁不同极性组分对油脂抗氧化作用研究[J].中国油脂,2016,41(5):45-49.
[22]徐连巧,曹剑锋,谢之英,等.响应面法优化小春花根黄酮提取工艺及其抗油脂氧化活性研究[J].食品安全质量检测学报,2018,9(5):985-992.
[23]王晓静,陈莉华,黄玉龙,等.火棘果黄酮对油脂的抗氧化活性[J].天然产物研究与开发,2015,27:909-914.
[24]关海宁,刁小琴,乔秀丽,等.提取方式对玉米须总多酚抗氧化活性的影响研究[J].食品工业,2018,39(8):25-29.
[25]张锦华,徐蔓,白宝清,等.响应面法优化提取无花果干果中多酚和总黄酮物质及其抗氧化活性[J].食品工业科技,2018,39(16):183-190.