华南风铃木类植物种质资源的形态与分子鉴定
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摘要
原产于中南美洲的风铃木类植物(紫葳科)因具有很高的观赏价值广泛栽培于华南地区。然而这些植物的种源目前还有疑问。本研究对栽培于华南的风铃木类植物进行了系统的形态和分子鉴定。结果表明,这7个分类单元的植物来自于2个属(Tabebuia和Handroanthus)的4个物种(T. rosea,T. aurea,H. chrysanthus和H. impetiginosa),其中H. chrysanthus包括2个亚种(H. chrysanthus subsp. chrysanthus和H. chrysanthus subsp. meridionalis)。其中,紫花/洋红/粉红风铃木在分子水平上无法区分,支持其作为一个物种。正确的物种鉴定为杂交育种、科学管理和园林应用奠定了基础。
Tabebuia and Handroanthus(Bignoniaceae) are native to central and south America,and widely cultivated in south China because of the high ornamental value,but the provenance of these plants is still unclear.We carried out the morphology and molecular identification of these plants cultivated in south China.The results indicated that:7 taxa belonged to 4 species from 2 genera(Tabebuia and Handroanthus),including T.rosea,T.aurea,H.impetiginosa and 2 subspecies of H.chrysanthus(H.chrysanthus subsp.chrysanthus and H.chrysanthus subsp.meridionalis).3 taxa with main difference in petal color could not be distinguished at the molecular level,supporting them as one species.Correct classification of these species will contribute to the hybrid breeding,scientific management and landscape application.
Tabebuia and Handroanthus(Bignoniaceae) are native to central and south America,and widely cultivated in south China because of the high ornamental value,but the provenance of these plants is still unclear.We carried out the morphology and molecular identification of these plants cultivated in south China.The results indicated that:7 taxa belonged to 4 species from 2 genera(Tabebuia and Handroanthus),including T.rosea,T.aurea,H.impetiginosa and 2 subspecies of H.chrysanthus(H.chrysanthus subsp.chrysanthus and H.chrysanthus subsp.meridionalis).3 taxa with main difference in petal color could not be distinguished at the molecular level,supporting them as one species.Correct classification of these species will contribute to the hybrid breeding,scientific management and landscape application.
引文
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[23]曾珠,许学洪,严冯权,等.热带北缘地区风铃木火焰木引种试验及大苗移栽技术[J].山东林业科技,2017(5):35-38.
[24]陈卫国.深圳南山区前海路黄花风铃木赏析[J].广东园林,2014,36(2):I0005-I0008.
[25]张静,廖绍波,孙冰,等.观赏树种黄花风铃木花期物候与花形态[J].浙江农林大学学报,2017,34(4):759-764.
[26] Doyle J, Doyle J L. Genomic plant DNA preparation from freshtissue-CTAB method[J].Phytochem Bull,1987,19(11):11-15.
[27] Taberlet P, Gielly L, Pautou G, et al. Universal primers foramplification of three non-coding regions of chloroplast DNA[J].Plant molecular biology,1991,17(5):1105-1109.
[28] Strand A E, Leebens-Mack J, Milligan B G. Nuclear DNA-basedmarkers for plant evolutionary biology[J].Molecular Ecology,1997,6(2):113-118.
[29] Librado P, Rozas J. DnaSP v5:a software for comprehensiveanalysis of DNA polymorphism data[J].Bioinformatics,2009,25(11):1451-1452.
[30] Tamura K, Stecher G, Peterson D, et al. MEGA6:molecularevolutionary genetics analysis version 6.0[J].Molecular biology andevolution,2013,30(12):2725-2729.
[31] Katoh K, Misawa K, Kuma K, et al. MAFFT:a novel method forrapid multiple sequence alignment based on fast Fourier transform[J].Nucleic acids research,2002,30(14):3059-3066.
[32] Ronquist F, Huelsenbeck J, Teslenko M. Draft MrBayes version 3.2manual:tutorials and model summaries[J].2011.
[2]王熙.园林树种黄花风铃木[J].广东林业科技,2007,23(2):115-116.
[3]林文胜.黄花风铃木的栽培技术研究及绿化应用[J].中国园艺文摘,2011,27(12):117-118.
[4]武丽琼.红花风铃木的引种栽培及应用[J].广东农业科学,2010,37(7):77-79.
[5] Mattos J R. Handroanthus, Um novo gênero para os‘‘ipês’’doBrasil[J].Loefgrenia,1970,50(2):1-4.
[6] Gentry A H. Handroanthus(Bigoniaceae):a critique[J].Taxon,1972,21(1):113-114.
[7] dos Santos G, Miller R B. Wood anatomy of Tecomeae[J].FloraNeotropica Monograph,1992,25:336-358.
[8] Spangler R E, Olmstead R G. Phylogenetic analysis ofBignoniaceae based on the cp DNA gene sequences rbcL and ndhF[J].Annals of the Missouri Botanical Garden,1999:33-46.
[9] Grose S O, Olmstead R G. Taxonomic revisions in the polyphyleticgenus Tabebuia s. I(Bignoniaceae)[J].Systematic Botany,2007,32(3):660-670.
[10] Grose S O, Olmstead R G. Evolution of a charismatic neotropicalclade:Molecular phylogeny of Tabebuia sl, Crescentieae, and alliedgenera(Bignoniaceae)[J].Systematic Botany,2007,32(3):650-659.
[11] Record S J, Hess R W. American timbers of the family Bignoniaceae[J].Tropical Woods,1940(63):9-38.
[12] Gentry A H. A synopsis of Bignoniaceae ethnobotany and economicbotany[M].Annals of the Missouri Botanical Garden,1992:53-64.
[13] Huxley A J, Griffiths M. New royal horticultural society dictionaryof gardening[M].Grove’s Dictionaries Inc.,1999.
[14] Staples G, Herbst D R. tropical garden flora[M].Bishop MuseumPress,2005.
[15] Mabberley D J. Mabberley’s Plant-Book third edition[M].Cambridge University Press,2008.
[16]蔡德才,方良,尚秀华,等.风铃木研究现状及开发利用前景[J].桉树科技,2016(3):46-50.
[17]尚秀华,高丽琼,张沛健,等.3种风铃木扦插繁殖技术研究[J].桉树科技,2016,33(1):38-42.
[18]钟海智,林耿,陈志刚,等.黄花风铃木种苗培育技术[J].广东林业科技,2015(2):146-148.
[19]李进华,林茂,孙开道,等.黄花风铃木园林用容器大苗培植关键技术[J].农业研究与应用,2016(2):75-77.
[20]黄宝华.黄花风铃木穴盘育苗基质筛选的研究[J].闽南师范大学学报:自然科学版,2016,29(4):29-32.
[21]王振师,许冲勇,曾雷,等.黄金风铃木,鸡冠刺桐和雄黄豆的引种与栽培[J].广东园林,2005(1):33-35.
[22]李玲莉,王宝宁,张鹏.黄花风铃木在重庆的引种栽培[J].园林科技,2016(1):11-15.
[23]曾珠,许学洪,严冯权,等.热带北缘地区风铃木火焰木引种试验及大苗移栽技术[J].山东林业科技,2017(5):35-38.
[24]陈卫国.深圳南山区前海路黄花风铃木赏析[J].广东园林,2014,36(2):I0005-I0008.
[25]张静,廖绍波,孙冰,等.观赏树种黄花风铃木花期物候与花形态[J].浙江农林大学学报,2017,34(4):759-764.
[26] Doyle J, Doyle J L. Genomic plant DNA preparation from freshtissue-CTAB method[J].Phytochem Bull,1987,19(11):11-15.
[27] Taberlet P, Gielly L, Pautou G, et al. Universal primers foramplification of three non-coding regions of chloroplast DNA[J].Plant molecular biology,1991,17(5):1105-1109.
[28] Strand A E, Leebens-Mack J, Milligan B G. Nuclear DNA-basedmarkers for plant evolutionary biology[J].Molecular Ecology,1997,6(2):113-118.
[29] Librado P, Rozas J. DnaSP v5:a software for comprehensiveanalysis of DNA polymorphism data[J].Bioinformatics,2009,25(11):1451-1452.
[30] Tamura K, Stecher G, Peterson D, et al. MEGA6:molecularevolutionary genetics analysis version 6.0[J].Molecular biology andevolution,2013,30(12):2725-2729.
[31] Katoh K, Misawa K, Kuma K, et al. MAFFT:a novel method forrapid multiple sequence alignment based on fast Fourier transform[J].Nucleic acids research,2002,30(14):3059-3066.
[32] Ronquist F, Huelsenbeck J, Teslenko M. Draft MrBayes version 3.2manual:tutorials and model summaries[J].2011.