星点设计-响应面法优化意大利牛舌草总皂苷提取工艺
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摘要
目的采用星点设计-响应面法优化意大利牛舌草总皂苷提取工艺。方法采用单因素试验研究乙醇浓度、液料比、回流提取时间及回流提取次数对意大利牛舌草总皂苷提取效果的影响。在单因素试验结果基础上,采用星点设计-响应面法优选意大利牛舌草总皂苷提取工艺。结果优选出的意大利牛舌草总皂苷提取工艺为:提取溶剂为80%乙醇,液料比14∶1(mL∶g),回流提取1 h,提取2次,总皂苷含量为(203.04±1.36)mg/g (RSD=0.67%),与预测值之间的相对误差为1.04%。结论星点设计-响应面法优化意大利牛舌草总皂苷提取工艺方法简便,结果可靠,预测性良好。
Objective To optimize the extraction process for total saponins from Anchusa italica Retz. by central composite design-response surface method. Methods Single factor test were conducted to evaluate the effects of ethanol concentration, liquid material ratio, reflux extraction time and reflux extraction times on the extraction of total saponins from Anchusa italica Retz. Based on the results of single factor test, the central composite design-response surface method was used to optimize the extraction process of total saponin from Anchusa italica Retz. Results The optimum extraction conditions were as follows: 80% ethanol concentration was selected as extraction, solvent, liquid material ratio was 14∶1(mL∶g), reflux extraction 1 h and reflux extraction twice, the total saponin content is(203.04±1.36) mg/g(RSD=0.67%). Conclusion The central composite design-response surface method is simple, reliable and highly predictive for the optimization of extraction technology of saponins from Anchusa italica Retz.
Objective To optimize the extraction process for total saponins from Anchusa italica Retz. by central composite design-response surface method. Methods Single factor test were conducted to evaluate the effects of ethanol concentration, liquid material ratio, reflux extraction time and reflux extraction times on the extraction of total saponins from Anchusa italica Retz. Based on the results of single factor test, the central composite design-response surface method was used to optimize the extraction process of total saponin from Anchusa italica Retz. Results The optimum extraction conditions were as follows: 80% ethanol concentration was selected as extraction, solvent, liquid material ratio was 14∶1(mL∶g), reflux extraction 1 h and reflux extraction twice, the total saponin content is(203.04±1.36) mg/g(RSD=0.67%). Conclusion The central composite design-response surface method is simple, reliable and highly predictive for the optimization of extraction technology of saponins from Anchusa italica Retz.
引文
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[11] 殷佳, 殷健, 朱艳, 等. 星点设计-响应面法优化补肾活血胶囊提取工艺[J].中国药房, 2016, 27(16):2276-2278.
[12] 刘颖新, 刘利利, 喻祖文,等. 星点设计-响应面法优化厚朴提取工艺[J]. 中国实验方剂学杂志, 2013, 19(7):27-30.
[13] 尚雅文, 龚慕辛, 翟永松, 等. 星点设计-效应面法优选糖肾宁颗粒的成型工艺[J]. 中国实验方剂学杂志, 2015, 21(16):14-18.
[14] 王健,曾吉祥,龚远林. 响应曲面法优化姜黄素包合物的制备 [J]. 川北医学院学报, 2015, 30(2):166-169.
[15] 吴伟, 崔光华, 陆彬. 实验设计中多指标的优化:星点设计和总评归一值的应用[J]. 中国药学杂志,2000, 35(8):530-533.
[2] ABBAS M, DISI A, AL-KHALIL S. Isolation and identification of antiulcer components from Anchusa strigosa root[J]. Jordan J Pharm Sci, 2009, 2(2):131-139.
[3] HOU Y Z, CHEN K K, DENG X L, et al. Anti-complementary constituents of Anchusa italica[J]. Nat Prod Res, 2017, 31(21):2572-2574.
[4] CHEN K K, XIE Z J, DAI W, et al. A new oleanolic-type triterpene glycoside from Anchusa italica[J]. Nat Prod Res, 2016, 31(8):959-965.
[5] KURUUZUMUZ A, SULEYMAN H, CADIRCI E, et al. Investigation on anti-inflammatory and antiulcer activities of Anchusa azurea extracts and their major constituent rosmarinic acid[J]. J of Bio Sci, 2012, 67(7/8):360-366.
[6] 谢周建, 戴沩, 黎梅, 等. 牛舌草活性部位的抗氧化和美白作用[J]. 中国实验方剂学杂志, 2014, 20(12):150-152.
[7] PAPADOYANNIS I N, SAMANIDOU V F, ASSIMOPOULOU A N, et al. Recent advances in chemistry, biology and biotechnology of Alkannins and Shikonins[J]. Current Organic Chem, 2006, 10(16):2123-2142.
[8] LIANG D, YONG S, SHEN Y, et al. Shikonin exerts anti-inflammatory effects in a murine model of lipopolysaccharide-induced acute lung injury by inhibiting the nuclear factor-kappa B signaling pathway[J]. Int Immunopharmacol, 2013, 16(4):475-480.
[9] 何媛媛, 王金凤, 郭丽娜, 等.意大利牛舌草化学成分及药理作用研究进展[J].环球中医药, 2017, 10(1):114-120.
[10] 鞠建峰, 朱宗敏, 杜忠科. 星点设计-效应面法优化重楼皂苷类成分的提取工艺[J].中国药房, 2015, 26(28):3967-3969.
[11] 殷佳, 殷健, 朱艳, 等. 星点设计-响应面法优化补肾活血胶囊提取工艺[J].中国药房, 2016, 27(16):2276-2278.
[12] 刘颖新, 刘利利, 喻祖文,等. 星点设计-响应面法优化厚朴提取工艺[J]. 中国实验方剂学杂志, 2013, 19(7):27-30.
[13] 尚雅文, 龚慕辛, 翟永松, 等. 星点设计-效应面法优选糖肾宁颗粒的成型工艺[J]. 中国实验方剂学杂志, 2015, 21(16):14-18.
[14] 王健,曾吉祥,龚远林. 响应曲面法优化姜黄素包合物的制备 [J]. 川北医学院学报, 2015, 30(2):166-169.
[15] 吴伟, 崔光华, 陆彬. 实验设计中多指标的优化:星点设计和总评归一值的应用[J]. 中国药学杂志,2000, 35(8):530-533.