鞑靼忍冬再生体系的建立
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摘要
以鞑靼忍冬的成熟种胚作为外植体,建立再生植株体系,结果表明:成熟种胚采用2%的NaClO溶液处理时,最佳消毒处理时间为6 min,存活率为75%。诱导愈伤组织以及诱导不定芽的最佳培养基为MS+2.0 mg/L 6-BA+0.1 mg/L NAA,愈伤组织诱导率为74.85%;不定芽诱导率为69.35%。增殖的最佳培养基为MS+2.0 mg/L 6-BA+0.3 mg/L NAA,增殖率为76.58%。诱导生根最佳培养基为1/2 MS+0.5 mg/L IBA,生根率为76.45%,平均根长为4.25 cm。通过组织培养再生植株,对其快速繁殖、种质资源保存和改良及规模化生产利用以及工厂化育苗技术研究提供理论基础。
In this paper, the mature embryo of tatar honeysuckle was used as explants to establish plant regeneration system. The results showed that when the mature embryo was treated with 2% NaClO solution, the best disinfection time was 6 min and the survival rate was 75%. The best medium for callus and adventitious bud induction was MS+2.0 mg/L 6-BA+0.1 mg/L NAA, with the callus induction rate of 74.85%, and adventitious bud induction rate of 69.35%. The best medium for proliferation was MS +2.0 mg/L 6-BA+0.3 mg/L NAA, and the proliferation rate was 76.58%. The best medium for root induction was 1/2 MS+0.5 mg/L IBA, rooting rate was76.45%, and the average root length was 4.25 cm. Plants regenerated by tissue culture could provide theoretical basis for rapid propagation, conservation and improvement of germplasm resources, large-scale production and utilization, as well as industrial seedling technology research of tatar honeysuckle.
In this paper, the mature embryo of tatar honeysuckle was used as explants to establish plant regeneration system. The results showed that when the mature embryo was treated with 2% NaClO solution, the best disinfection time was 6 min and the survival rate was 75%. The best medium for callus and adventitious bud induction was MS+2.0 mg/L 6-BA+0.1 mg/L NAA, with the callus induction rate of 74.85%, and adventitious bud induction rate of 69.35%. The best medium for proliferation was MS +2.0 mg/L 6-BA+0.3 mg/L NAA, and the proliferation rate was 76.58%. The best medium for root induction was 1/2 MS+0.5 mg/L IBA, rooting rate was76.45%, and the average root length was 4.25 cm. Plants regenerated by tissue culture could provide theoretical basis for rapid propagation, conservation and improvement of germplasm resources, large-scale production and utilization, as well as industrial seedling technology research of tatar honeysuckle.
引文
Fang G.Q.,Jiang J.P.,He Y.Q.,and Li F.,2003,In vitro efficient plant regeneration with Platanus orientalis L.leaves as explants,Yuanyi Xuebao(Acta Horticulturae Sinica),30(2):236-238(范国强,蒋建平,贺窑青,李锋,2003,悬铃木叶片植株再生系统的建立,园艺学报,30(2):236-238)
Huang D.L.,Geng S.L.,Li X.S.,and Xu H.H.,2001,Studies on medicinal plant resources of Genera lonicera L.in Guangdong Province,Guangdong Yaoxueyuan Xuebao(Academic Journal of Guangdong College of Pharmacy),18(3):177-180(黄东亮,耿世磊,李学松,徐鸿华,2002,广东省忍冬属药用植物资源研究,广东药学院学报,18(3):177-180)
Li S.C.,Li J.,Tong F.Q.,Luan L.,and Yu Q.L.,1995,Studies on tissue culture of Populus davidiana and Pussuritnsls and Ptomentose,Liaoning Daxue Xuebao(Journal of Liaoning University(Natural Sciences Edition)),22(2):59-62(李世承,李晶,佟凤琴,栾岚,于清兰,1995,山杨、大青杨、毛白杨组织培养的研究,辽宁大学学报(自然科学版),22(2):59-62)
Lin Y.D.,2001,On sustainable development of urban green space,Zhongguo Yuanlin(Chinese Garden),(6):29-31(蔺银鼎,2001,对城市园林绿地可持续发展的思考,中国园林,(6):29-31)
Sun X.G.,Xu X.H.,Jiao J.,and Peng Z.X.,1997,Systematic classification of the genus Lonicera L.from Gansu,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),17(6):190-198(孙学刚,徐向宏,焦健,彭泽祥,1997,甘肃省忍冬属植物系统分类,西北植物学报,17(6):190-198)
Tang W.,and Ouyang F.,1998,Studies on embryogenic callus induction and plant regeneration in loblolly pine,Linye Kexue(Scientia Silvae Sinicae),34(3):115-119(唐巍,欧阳藩,1998,火炬松胚性愈伤组织诱导和植株再生的研究,林业科学,34(3):115-119)
Wang S.,Jia Y.J.,Wei F.,Lu H.Z.,and Wu S.F.,1997,Tissue culture and plant regeneration of Taxus yunnanensis,Yunnan Zhiwu Yanjiu(Acta Botanica Yunnanica),19(4):407-410(王水,贾勇炯,魏峰,卢慧宗,伍顺凤,1997,云南红豆杉的组织培养及植株再生,云南植物研究,19(4):407-410)
Zhang E.H.,1987,Differentiation of flower buds of a whole honeysuckle plant,Zhongguo Yaoke Daxue Xuebao(Journal of China Pharmaceutical University),19(1):8-12(张恩汉,1987,忍冬全株花芽的分化发育,中国药科大学学报,19(1):8-12)
Zhu C.S.,1995,Preliminary study on Lonicear from Henan,Henan Nongye Daxue Xuebao(Aeta Agrieulturae Universitatis Henanensis),29(1):38-44(朱长山,1995,河南省忍冬属植物的初步研究,河南农业大学学报,29(1):38-44)
Huang D.L.,Geng S.L.,Li X.S.,and Xu H.H.,2001,Studies on medicinal plant resources of Genera lonicera L.in Guangdong Province,Guangdong Yaoxueyuan Xuebao(Academic Journal of Guangdong College of Pharmacy),18(3):177-180(黄东亮,耿世磊,李学松,徐鸿华,2002,广东省忍冬属药用植物资源研究,广东药学院学报,18(3):177-180)
Li S.C.,Li J.,Tong F.Q.,Luan L.,and Yu Q.L.,1995,Studies on tissue culture of Populus davidiana and Pussuritnsls and Ptomentose,Liaoning Daxue Xuebao(Journal of Liaoning University(Natural Sciences Edition)),22(2):59-62(李世承,李晶,佟凤琴,栾岚,于清兰,1995,山杨、大青杨、毛白杨组织培养的研究,辽宁大学学报(自然科学版),22(2):59-62)
Lin Y.D.,2001,On sustainable development of urban green space,Zhongguo Yuanlin(Chinese Garden),(6):29-31(蔺银鼎,2001,对城市园林绿地可持续发展的思考,中国园林,(6):29-31)
Sun X.G.,Xu X.H.,Jiao J.,and Peng Z.X.,1997,Systematic classification of the genus Lonicera L.from Gansu,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),17(6):190-198(孙学刚,徐向宏,焦健,彭泽祥,1997,甘肃省忍冬属植物系统分类,西北植物学报,17(6):190-198)
Tang W.,and Ouyang F.,1998,Studies on embryogenic callus induction and plant regeneration in loblolly pine,Linye Kexue(Scientia Silvae Sinicae),34(3):115-119(唐巍,欧阳藩,1998,火炬松胚性愈伤组织诱导和植株再生的研究,林业科学,34(3):115-119)
Wang S.,Jia Y.J.,Wei F.,Lu H.Z.,and Wu S.F.,1997,Tissue culture and plant regeneration of Taxus yunnanensis,Yunnan Zhiwu Yanjiu(Acta Botanica Yunnanica),19(4):407-410(王水,贾勇炯,魏峰,卢慧宗,伍顺凤,1997,云南红豆杉的组织培养及植株再生,云南植物研究,19(4):407-410)
Zhang E.H.,1987,Differentiation of flower buds of a whole honeysuckle plant,Zhongguo Yaoke Daxue Xuebao(Journal of China Pharmaceutical University),19(1):8-12(张恩汉,1987,忍冬全株花芽的分化发育,中国药科大学学报,19(1):8-12)
Zhu C.S.,1995,Preliminary study on Lonicear from Henan,Henan Nongye Daxue Xuebao(Aeta Agrieulturae Universitatis Henanensis),29(1):38-44(朱长山,1995,河南省忍冬属植物的初步研究,河南农业大学学报,29(1):38-44)