外源亚精胺防御红椿干旱胁迫生理损伤的最佳浓度筛选
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摘要
为了筛选出外源亚精胺防御红椿干旱胁迫生理损伤的最佳浓度,以2年生红椿家系盆栽苗为研究对象,开展了外源Spd多浓度预处理+干旱胁迫处理试验,并对叶片相对含水量、叶绿素含量、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、游离脯氨酸含量和丙二醛含量等6个生理指标的观测数据和最佳浓度进行了综合研究,得出以下结果:1)4种外源亚精胺溶液对6个生理指标在不同干旱胁迫处理下的防御效果均达到了极显著差异(P <0.01)。2)1 mmol/L和2 mmol/L外源Spd预处理对植株叶片相对含水量、叶绿素含量、游离脯氨酸含量和丙二醛含量等4个生理指标的防御效果更好,且均遵守干旱胁迫程度越轻则生理损伤防御效果越好的规律;而不同浓度外源Spd预处理对植株超氧化物歧化酶(SOD)活性和过氧化物酶(POD)活性的防御影响并未发现明显规律。3)运用模糊隶属函数法对4种外源Spd浓度进行筛选,发现2 mmol/L外源Spd溶液的综合评价值最大,为4种参试浓度中的最优浓度。
A experiment of "exogenous Spd pretreatment with multiple concentrations + drought stress" was carried out with twoyear potted seedlings of Toona ciliata families in order to screen out the optimum concentration of exogenous Spd on defending against physiological damage of Toona ciliata under drought stress. Observational data and optimal concentration of six physiological indexes including leaf relative water content, chlorophyll content, superoxide dismutase(SOD) activity, peroxidase(POD) activity,free proline content and malondialdehyde content were comprehensively studied. The results are as follows. 1)There were generally extremely significant differences in defense effect of 4 exogenous Spd concentrations on 6 physiological parameters(P < 0.01). 2) The pretreatment of 1 mmol/L and 2 mmol/L exogenous Spd had better defense effects on the relative physiological parameters of water,chlorophyll content, free proline content and malondialdehyde content, all of them followed the rule that the less drought stress was, the better the physiological damage defense effect would be; However, the effects of different concentrations of exogenous Spd pretreatment on the activities of superoxide dismutase(SOD) and peroxidase(POD) activities were not found. 3) Four kinds of exogenous Spd concentrations were screened by fuzzy membership function method. It was found that the comprehensive evaluation value of 2 mmol/L exogenous Spd solution was the largest, which was the optimal concentration among the four tested concentrations.
A experiment of "exogenous Spd pretreatment with multiple concentrations + drought stress" was carried out with twoyear potted seedlings of Toona ciliata families in order to screen out the optimum concentration of exogenous Spd on defending against physiological damage of Toona ciliata under drought stress. Observational data and optimal concentration of six physiological indexes including leaf relative water content, chlorophyll content, superoxide dismutase(SOD) activity, peroxidase(POD) activity,free proline content and malondialdehyde content were comprehensively studied. The results are as follows. 1)There were generally extremely significant differences in defense effect of 4 exogenous Spd concentrations on 6 physiological parameters(P < 0.01). 2) The pretreatment of 1 mmol/L and 2 mmol/L exogenous Spd had better defense effects on the relative physiological parameters of water,chlorophyll content, free proline content and malondialdehyde content, all of them followed the rule that the less drought stress was, the better the physiological damage defense effect would be; However, the effects of different concentrations of exogenous Spd pretreatment on the activities of superoxide dismutase(SOD) and peroxidase(POD) activities were not found. 3) Four kinds of exogenous Spd concentrations were screened by fuzzy membership function method. It was found that the comprehensive evaluation value of 2 mmol/L exogenous Spd solution was the largest, which was the optimal concentration among the four tested concentrations.
引文
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[2]SMITH T A.Polyamines[J].Annu.Rev.Plant Physiol.Biochem.J.,1985,249:33-36.
[3]ROY P,NIYOGI K,SENGUPTA D N.Spermindine treatment to rice seedlings recovers salinity stress-induced damage of plasma membrane and PM-bound H+-ATPase in salt-tolerant and saltsensitive rice cultivar[J].Plant Science,2005,168:583-591.
[4]郭双,卢克欢,张翠平,等.外源亚精胺对盐胁迫下颠茄生理特性和生物碱积累的影响[J].西南大学学报(自然科学版),2018,40(1):43-49.
[5]张毅,石玉,胡晓辉.外源亚精胺对盐碱胁迫下番茄幼苗光合特性的影响[J].西北农林科技大学学报(自然科学版),2016,44(2):144-150.
[6]杜红阳,胡春红,刘怀攀.外源亚精胺对盐胁迫下大豆幼苗叶片光合性能和抗氧化代谢的效应[J].大豆科学,2016,35(1):80-85.
[7]梅燚,祖艳侠,王薇薇,等.外源亚精胺对高温胁迫下萝卜幼苗生长发育的影响[J].中国农学通报,2017,33(31):33-38.
[8]张永平,许爽,杨少军,等.外源亚精胺对低温胁迫下甜瓜幼苗生长和抗氧化系统的影响[J].植物生理学报,2017,53(6):1087-1096.
[9]申璐.外源亚精胺对铅胁迫下茶树生长的影响[D].杨凌:西北农林科技大学,2014.
[10]袁菊红,胡锦好.外源亚精胺对受重金属污染的城市污泥植物修复的影响[J].水土保持学报,2011,25(5):186-190.
[11]黄拯,钟秋平,曹林青,等.干旱胁迫对油茶成林光合作用的影响[J].经济林研究,2017,35(4):72-79.
[12]任余艳,赵雨兴,刘朝霞,等.干旱半干旱地区影响文冠果育苗的主导因子分析[J].经济林研究,2017,35(3):1-12,23.
[13]高志明.外源亚精胺对葡萄耐旱性的影响[J].中国南方果树,2018,47(1):108-110,113.
[14]吴旭红,冯晶旻.外源亚精胺对PEG胁迫下燕麦幼苗生长及部分生理特性的影响[J].麦类作物学报,2017,37(1):122-129.
[15]程明明,杜红阳,刘红攀.外源亚精胺对涝胁迫下玉米幼苗根的氧化伤害缓解效应[J].南方农业学报,2015,46(1):36-41.
[16]王强,徐洪峰,郝梦霞.外源亚精胺对干旱胁迫下白刺花幼苗生长的影响[J].浙江农业学报,2014,26(4):954-960.
[17]僧珊珊,王群,张永恩,等.外源亚精胺对淹水胁迫玉米的生理调控效应[J].作物学报,2012,38(6):1042-1050.
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[19]刘球,李志辉,吴际友,等.红椿幼苗对干旱胁迫及复水生理响应的典型相关分析[J].西北农林科技大学学报(自然科学版),2015,43(10):1-10.
[20]湛欣,鲁好君,赵帅,等.红椿SSR-PCR体系建立和多态性引物筛选[J].林业科学研究,2016,29(4):565-570.
[21]吴际友,李志辉,刘球,等.干旱胁迫对红椿无性系幼苗叶片相对含水量和叶绿素含量的影响[J].中国农学通报,2013,29(4):19-22.
[22]陈彩霞,王瑞辉,吴际友,等.持续干旱条件下红椿无性系幼苗的生理响应[J].中南林业科技大学学报,2013,33(9):46-49.
[23]陈彩霞,王瑞辉,吴际友,等.淹水胁迫对红椿家系幼苗几种生理生化指标的影响[J].西北林学院学报,2014,29(3):21-26.
[24]刘球,李志辉,吴际友,等.持续干旱胁迫下外源精胺对红椿幼苗的形态与生理调节效应的影响[J].生态学杂志,2016,35(12):3266-3272.
[25]刘球,李志辉,吴际友,等.外源亚精胺对不同干旱胁迫程度下红椿幼苗生理功能的修复调节[J].中南林业科技大学学报,2017,37(7):66-72.
[26]陈建勋,王晓峰.植物生理学实验指导[M].2版.广州:华南理工大学出版社,2006.
[27]宋洪元,雷建军,李成琼.植物热胁迫反应及抗热性鉴定与评价[J].中国蔬菜,1998(1):48-50.
[28]李丽杰,顾万荣,张倩,等.外源亚精胺对干旱胁迫下玉米幼苗叶片生理及根系特征参数的影响[J].农药学学报,2015,17(3):291-299.
[29]逯明辉,王晓妮,巩振辉.外源亚精胺对辣椒幼苗干旱胁迫的缓解效应[J].辣椒杂志,2014(4):40-44.