玉米o2、o16和wx基因不同组合类型近等基因系品质分析
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摘要
为评价o2、o16和wx基因不同组合类型近等基因系的品质,以玉米o2、o16和wx基因不同组合类型近等基因系BC 6 F 4家系及其轮回亲本为试材,利用近红外分析仪和氨基酸全自动分析仪对籽粒油分、淀粉、蛋白质和氨基酸含量进行分析。结果表明,与轮回亲本CML 539相比,o2o16wx-NIL、o2o16-NIL、o2wx-NIL和o2-NIL籽粒油分含量分别提高了11.37%、8.86%、17.53%和11.95%。o2wx-NIL和o2-NIL籽粒淀粉含量分别降低了0.71%和1.00%。o16wx-NIL和wx-NIL蛋白质含量分别提高了9.91%和10.22%,o2wx-NIL、o16wx-NIL和o16-NIL蛋白质含量分别降低了6.03%、6.05%和5.87%。o2o16wx-NIL,o2o16-NIL,o2wx-NIL,o16wx-NIL,o2-NIL、wx-NIL和o16-NIL氨基酸含量均发生不同程度变化,其中,Lys分别增加了111.22%、109.53%、91.22%、34.40%、79.93%、29.69%和70.11%。可见,o2、o16和wx基因不同组合类型近等基因系是品质基因遗传研究和育种应用的重要材料。
In order to evaluate the nutritional quality of the near-isogenic lines(NILs) of the different combination types of o2,o16 and wx genes,the seed oil,starch,protein and amino acid contents were measured by the near-infrared and automatic amino acid analyzers,using the BC 6 F 4 families of those NILs and their recurrent parent as materials.The results showed that,compared with the recurrent parent CML 539,the seed oil contents of o2 o16 wx-NIL,o2 o16-NIL,o2 wx-NIL and o2-NIL were increased by 11.37%,8.86%,17.53% and 11.95%,respectively.The seed starch contents of o2 wx-NIL and o2-NIL were decreased by 0.71% and 1.00%,respectively.The seed protein contents of o16 wx-NIL and wx-NIL were increased by 9.91% and 10.22%,while those of o2 wx-NIL,o2-NIL and o16-NIL were decreased by 6.03%,6.05% and 5.87%,respectively.The seed lysine contents of o2 o16 wx-NIL,o2 o16-NIL,o2 wx-NIL,o16 wx-NIL,o2-NIL,wx-NIL and o16-NIL were increased by 111.22%,109.53%,91.22%,34.40%,79.93%,29.69% and 70.11%,respectively.It can be seen that the near isogenic lines of different combinations of o2,o16 and wx genes are important materials for genetic research and breeding application of quality genes.
In order to evaluate the nutritional quality of the near-isogenic lines(NILs) of the different combination types of o2,o16 and wx genes,the seed oil,starch,protein and amino acid contents were measured by the near-infrared and automatic amino acid analyzers,using the BC 6 F 4 families of those NILs and their recurrent parent as materials.The results showed that,compared with the recurrent parent CML 539,the seed oil contents of o2 o16 wx-NIL,o2 o16-NIL,o2 wx-NIL and o2-NIL were increased by 11.37%,8.86%,17.53% and 11.95%,respectively.The seed starch contents of o2 wx-NIL and o2-NIL were decreased by 0.71% and 1.00%,respectively.The seed protein contents of o16 wx-NIL and wx-NIL were increased by 9.91% and 10.22%,while those of o2 wx-NIL,o2-NIL and o16-NIL were decreased by 6.03%,6.05% and 5.87%,respectively.The seed lysine contents of o2 o16 wx-NIL,o2 o16-NIL,o2 wx-NIL,o16 wx-NIL,o2-NIL,wx-NIL and o16-NIL were increased by 111.22%,109.53%,91.22%,34.40%,79.93%,29.69% and 70.11%,respectively.It can be seen that the near isogenic lines of different combinations of o2,o16 and wx genes are important materials for genetic research and breeding application of quality genes.
引文
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[15]杨文鹏,王明春,王伟,等.玉米高赖氨酸突变奥帕克-2和奥帕克-16近等基因系的营养品质分析[J].贵州农业科学,2012(8):8-10.
[16]张晓星,朱慧,张东民,等.糯玉米opaque2基因近等基因系的创制[J].作物学报,2017,43(12):1760-1766.
[17]谭华,郑德波,邹成林,等.利用分子标记辅助选择优质蛋白玉米近等基因系[J].中国农学通报,2015,31(15):146-150.
[2]Mertz E T,Bates L S,Nelson O E.Mutant gene that changes protein composition and increases lysine content of maize endosperm[J].Science,1964,145,279-280.DOI:10.1126/science.145.3629.279.
[3]曾孟潜.我国糯质玉米的亲缘关系[J].作物品种资源,1987,3,21-24.DOI:10.19462/j.cnki.1671-895x.1987.03.004.
[4]Collins G N.A new type of Indian corn from China[J].U.S.Dapt.Agric.Bur.Plant Indus.Bull.1909,161,1-30.
[5]Wicker T,Sabot F,Hua-Van A,et al.A unified classification system for eukaryotic transposable elements[J].Nat.Rev.Genet.,2007,8:973-982.DOI:https://doi.org/10.1038/nrg2165.
[6]Fan L J,Quan L Y,Leng X D,et al.Molecular evidence for post-domestication selection in the Waxy gene of Chinese waxy maize[J].Mol.Breeding,2008,22:329-338.DOI:https://doi.org/10.1007/s11032-008-9178-2.
[7]Bao J D,Yao J Q,Zhu J Q Hu,et al.Identification of glutinous maize landraces and inbred lines with altered transcription of waxy gene[J].Mol.Breeding,2012,30:1707-1714.DOI:https://doi.org/10.1007/s11032-012-9754-3.
[8]Lu D,Lu WP.Effects of protein removal on the physicochemical properties of waxy maize flours[J].Starch-St?rke,2012,64:874-881.DOI:https://doi.org/10.1002/star.201200038.
[9]Zhang W L,Yang W P,Wang M C,et al.Increasing lysine content of waxy maize through introgression of opaque-2 and opaque-16 genes using molecular assisted and biochemical development[J].PLoS ONE,2013,8,e56227.DOI:10.1371/journal.pone.0056227.
[10]Zhou Z Q,Song L Y,Zhang X X,et al.Introgression of opaque 2 into waxy maize causes extensive biochemical and proteomic changes in endosperm[J].PLoS ONE,2016,11,e0158971.DOI:https://doi.org/10.1371/journal.pone.0158971.
[11]Misra P S,Mertz E T,Glover D V.Studies on corn proteins.VI.Endosperm protein changes in single and double endosperm mutants of maize[J].Cereal Chem.,1975,52:1-6.
[12]张述宽,滕辉升,苏琪,等.应用SSR辅助选择技术选育优质蛋白糯玉米自交系[J].广西农业科学,2009,40(10):1279-1283.
[13]Yang L Q,Wang W,Yang W P,et al.Marker-assisted selection for pyramiding the waxy and opaque-16 genes in maize using cross and backcross schemes[J].Mol.Breeding 2013,31:767-775.DOI:10.1007/s11032-012-9830-8.
[14]王伟,杨文鹏,王明春.优质蛋白糯玉米自交系的选育及品质分析[J].种子,2017,36(4):105-108.
[15]杨文鹏,王明春,王伟,等.玉米高赖氨酸突变奥帕克-2和奥帕克-16近等基因系的营养品质分析[J].贵州农业科学,2012(8):8-10.
[16]张晓星,朱慧,张东民,等.糯玉米opaque2基因近等基因系的创制[J].作物学报,2017,43(12):1760-1766.
[17]谭华,郑德波,邹成林,等.利用分子标记辅助选择优质蛋白玉米近等基因系[J].中国农学通报,2015,31(15):146-150.
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