杜仲胶复合义齿软衬材料制备及吸水性和溶解性检测
|
摘要
背景:目前在临床上使用最普遍的软衬为硅橡胶类和丙烯酸树脂类,但2种材料均存在一定的不足,因此开发性质稳定、性能优良、兼具抗菌性的软衬材料具有重要临床意义。目的:制备新型杜仲胶复合义齿软衬材料,同时评价其吸水性和溶解性。方法:参考现有弹性杜仲胶配方和医用橡胶配方,结合义齿软衬材料的特殊要求,调整杜仲胶与顺丁橡胶的比例(混炼比例分别为10∶90,30∶70,50∶50,70∶30,90∶10),制备新型杜仲胶复合义齿软衬材料,以丙烯酸自凝软衬材料和Silagum硅橡胶义齿软衬材料为对照,将各组样品分别置入人工唾液(或蒸馏水)中7,30 d,检测样品吸水性与溶解性。结果与结论:(1)在人工唾液中:第7,30天,各实验组吸水率、溶解率均与硅橡胶组接近,远低于丙烯酸酯组;(2)在蒸馏水中:第7,30天,各实验组吸水率、溶解率均与硅橡胶组接近,远低于丙烯酸酯组;(3)在人工唾液或蒸馏水中:丙烯酸酯组浸泡第7,30天的吸水率、溶解率大于其余各组(P <0.05);(4)浸泡第7,30天时,同种试件在人工唾液中的吸水率低于在蒸馏水中的吸水率(P <0.05);浸泡第7天时,仅30∶70组、50∶50组试件在人工唾液中的溶解率低于在蒸馏水中的溶解率(P<0.05);浸泡第30天时,30∶70组、50∶50组、70∶30组、90∶10组试件在人工唾液中的溶解率低于在蒸馏水中的溶解率(P<0.05);(5)在人工唾液与蒸馏水中:同种试件浸泡第30天的吸水率均大于第7天的吸水率(P<0.05);(6)在人工唾液中,50∶50组浸泡第7,30天的溶解率比较无差异(P> 0.05),70∶30组浸泡第7,30天的溶解率比较无差异(P>0.05),其他组组内不同时间点的溶解率比较差异显著(P <0.05);在蒸馏水中,10∶90组浸泡第7,30天的溶解率比较无差异(P> 0.05),30∶70组浸浸泡第7,30天的溶解率比较无差异(P> 0.05),其他组组内不同时间点的溶解率比较差异显著(P <0.05);(7)结果表明,杜仲胶复合义齿软衬材料的吸水性与Silagum硅橡胶软衬材料接近,显著低于丙烯酸酯软衬材料,且溶解性维持在较低水平。
BACKGROUND: The most common soft liners currently used in the clinic are silicone rubbers and acrylics, but both of them have deficiencies. Therefore, the development of soft lining materials with stability, excellent performance, and antibacterial properties is of important clinical significance. OBJECTIVE: To prepare a soft lining material with high elasticity on the basis of excellent characteristics of Eucommia ulmoides gum, and to preliminarily explore its water absorption and solubility. METHODS: The basic formula was made based on the existing formula of elastic Eucommia ulmoides gum and medical rubber, in combination with the specific requirements of denture lining materials. The ratio of Eucommia ulmoides to butadiene rubber was set to be 10:90, 30:70, 50:50, 70:30 and 90:10, and the new Eucommia ulmoides gum composite soft denture liners were then prepared. Acrylate self-curing soft lining material and Silagum silicone rubber denture soft lining material served as controls. Afterwards, the samples were put into distilled water or artificial saliva for 7 and 30 days, and the value of water absorption and water solubility were calculated. RESULTS AND CONCLUSION:(1) In the artificial saliva for 7 and 30 days, the water absorption rate and solubility of samples in different ratio groups were close to those in the Silagum group, but extremely lower than those in the acrylate group.(2) In the distilled water for 7 and 30 days, the water absorption rate and solubility of samples in different ratio groups were close to those in the Silagum group, but extremely lower than those in the acrylate group.(3) The water absorption rate and solubility of the acrylate group were significantly higher than those in the other groups at 7 and 30 days of immersion in the artificial saliva or distilled water(P < 0.05).(4) After 7 and 30 days of immersion, the water absorption rate of the same sample in the artificial saliva was significantly lower than that in the distilled water. Wile at 7 days of immersion, the samples in the 30:70 and 50:50 groups showed a lower solubility in the artificial saliva than in the distilled water (P < 0.05), and at 30 days of immersion, the samples in the 30:70, 50:50, 70:30 and 90:10 groups showed a lower solubility in the artificial saliva than in the distilled water (P < 0.05).(5) In the artificial saliva, there was no significant difference in the solubility in the 50:50 and 70:30 groups at 7 and 30 days of immersion (P > 0.05), while the solubility in the other groups exhibited significant differences at different observational times(P < 0.05). In the distilled water, there was no significant difference in the solubility in the 10:90 and 30:70 groups at 7 and 30 days of immersion (P > 0.05), while the solubility in the other groups exhibited significant differences at different observational times (P < 0.05). To conclude, the water absorption of Eucommia ulmoides gum composite soft denture liner is close to Silagum, but significantly less than that of acrylate soft lining materials, and the solubility remains at a very low level.
BACKGROUND: The most common soft liners currently used in the clinic are silicone rubbers and acrylics, but both of them have deficiencies. Therefore, the development of soft lining materials with stability, excellent performance, and antibacterial properties is of important clinical significance. OBJECTIVE: To prepare a soft lining material with high elasticity on the basis of excellent characteristics of Eucommia ulmoides gum, and to preliminarily explore its water absorption and solubility. METHODS: The basic formula was made based on the existing formula of elastic Eucommia ulmoides gum and medical rubber, in combination with the specific requirements of denture lining materials. The ratio of Eucommia ulmoides to butadiene rubber was set to be 10:90, 30:70, 50:50, 70:30 and 90:10, and the new Eucommia ulmoides gum composite soft denture liners were then prepared. Acrylate self-curing soft lining material and Silagum silicone rubber denture soft lining material served as controls. Afterwards, the samples were put into distilled water or artificial saliva for 7 and 30 days, and the value of water absorption and water solubility were calculated. RESULTS AND CONCLUSION:(1) In the artificial saliva for 7 and 30 days, the water absorption rate and solubility of samples in different ratio groups were close to those in the Silagum group, but extremely lower than those in the acrylate group.(2) In the distilled water for 7 and 30 days, the water absorption rate and solubility of samples in different ratio groups were close to those in the Silagum group, but extremely lower than those in the acrylate group.(3) The water absorption rate and solubility of the acrylate group were significantly higher than those in the other groups at 7 and 30 days of immersion in the artificial saliva or distilled water(P < 0.05).(4) After 7 and 30 days of immersion, the water absorption rate of the same sample in the artificial saliva was significantly lower than that in the distilled water. Wile at 7 days of immersion, the samples in the 30:70 and 50:50 groups showed a lower solubility in the artificial saliva than in the distilled water (P < 0.05), and at 30 days of immersion, the samples in the 30:70, 50:50, 70:30 and 90:10 groups showed a lower solubility in the artificial saliva than in the distilled water (P < 0.05).(5) In the artificial saliva, there was no significant difference in the solubility in the 50:50 and 70:30 groups at 7 and 30 days of immersion (P > 0.05), while the solubility in the other groups exhibited significant differences at different observational times(P < 0.05). In the distilled water, there was no significant difference in the solubility in the 10:90 and 30:70 groups at 7 and 30 days of immersion (P > 0.05), while the solubility in the other groups exhibited significant differences at different observational times (P < 0.05). To conclude, the water absorption of Eucommia ulmoides gum composite soft denture liner is close to Silagum, but significantly less than that of acrylate soft lining materials, and the solubility remains at a very low level.
引文
[1]刘慧东,马志刚,朱景乐,等.采用滤袋技术快速测定杜仲叶片中杜仲胶含量[J].天然产物研究与开发,2016,28(4):498-504.
[2]付文,刘安华,王丽.杜仲胶的提取与应用研究进展[J].弹性体,2014,24(5):76-80.
[3]张蕊,张洵箐,杨凤,等.杜仲胶改性高聚物的研究进展[J].高分子通报,2015,28(8):63-68.
[4]马娟,林永慧,刘彪,等.我国杜仲胶的发展现状与展望[J].安徽农业科学,2012,40(6):3396-3398.
[5]Tzunghsun T,Tsunghsien T,Youchia C,et al.In vitro antimicrobial activities against cariogenic streptococci and their antioxidant capacities:A comparative study of green tea versus different herbs.Food Chemi.2008;110(4):859-864.
[6]严瑞芳,胡汉杰.杜仲胶的研究与开发[J].中国科学基金,1994,8(1):55-59.
[7]McCabe JF.A polyvinylsiloxane denture soft lining material.JDent.1998;26(5-6):521-526.
[8]McCabe JF,Carrick TE,Kamohara H.Adhesive bond strength and compliance for denture soft lining materials.Biomaterials.2002;23(5):1347-1352.
[9]Mutluay MM,Ruyter IE.Evaluation of bond strength of soft relining materials to denture base polymers.Dent Mater.2007;23(11):1373-1381.
[10]Valentini F,Luz MS,Boscato N,et al.Biofilm formation on denture liners in a randomised controlled in situ trial.J Dent.2013;41(5):420-427.
[11]Zhou L,Tong Z,Wu G,et al.Parylene coating hinders Candida albicans adhesion to silicone elastomers and denture bases resin.Arch Oral Biol.2010;55(6):401-409.
[12]Dorocka-Bobkowska B,Medynski D,Prylinski M.Recent Advances in Tissue Conditioners for Prosthetic Treatment.Areview.Adv Clin Exp Med.2017;26(4):723-728.
[13]Craig RG,Gibbons P.Properties of resilient denture liners.JAm Dent Assoc.1961;63:382-390.
[14]辛振祥,张殿荣.现代橡胶配方设计[M].北京:化学工业出版社,2001.
[15]Altinci P,Mutluay M,S?derling E,et al.Antimicrobial efficacy and mechanical properties of BAC-modified hard and soft denture liners.Odontology.2017;106(1):1-7.
[16]De CF,Ayres M,Feltrin GP,et al.Effect of surface characteristics of soft liners and tissue conditioners and saliva on the adhesion and biofilm formation.Am J Dent.2018;31(1):45-52.
[17]Radi IA,Elmahrouky N.Effect of two different soft liners and thicknesses mediating stress transfer for immediately loaded2-implant supported mandibular overdentures:A finite element analysis study.J Prosthet Dent.2016;116(3):356-361.
[18]Sudhapalli S,Sudhapalli S.Time Dependent Effect of a Denture Cleanser on the Sorption and Solubility of Four Soft Liners-An Invitro Study.J Clin Diagn Res.2016;10(4):ZC100-103.
[19]Mello LJ,Gomes MJ,Juliana H,et al.Porosity of temporary denture soft liners containing antifungal agents.J Appl Oral Sci.2016;24(5):453-461.
[20]Mancuso DN,Goiato MC,Moreno A,et al.Effect of thermocycling on hardness,absorption,solubility and colour change of soft liners.Gerodontology.2012;29(2):e215-e219.
[21]Kostoulas I,Polyzois G,Mitsoudis A,et al.The effect of accelerated ageing on colour stability of visible light-cured(VLC)chairside denture liners.Gerodontology.2012;29(2):e239-e245.
[2]付文,刘安华,王丽.杜仲胶的提取与应用研究进展[J].弹性体,2014,24(5):76-80.
[3]张蕊,张洵箐,杨凤,等.杜仲胶改性高聚物的研究进展[J].高分子通报,2015,28(8):63-68.
[4]马娟,林永慧,刘彪,等.我国杜仲胶的发展现状与展望[J].安徽农业科学,2012,40(6):3396-3398.
[5]Tzunghsun T,Tsunghsien T,Youchia C,et al.In vitro antimicrobial activities against cariogenic streptococci and their antioxidant capacities:A comparative study of green tea versus different herbs.Food Chemi.2008;110(4):859-864.
[6]严瑞芳,胡汉杰.杜仲胶的研究与开发[J].中国科学基金,1994,8(1):55-59.
[7]McCabe JF.A polyvinylsiloxane denture soft lining material.JDent.1998;26(5-6):521-526.
[8]McCabe JF,Carrick TE,Kamohara H.Adhesive bond strength and compliance for denture soft lining materials.Biomaterials.2002;23(5):1347-1352.
[9]Mutluay MM,Ruyter IE.Evaluation of bond strength of soft relining materials to denture base polymers.Dent Mater.2007;23(11):1373-1381.
[10]Valentini F,Luz MS,Boscato N,et al.Biofilm formation on denture liners in a randomised controlled in situ trial.J Dent.2013;41(5):420-427.
[11]Zhou L,Tong Z,Wu G,et al.Parylene coating hinders Candida albicans adhesion to silicone elastomers and denture bases resin.Arch Oral Biol.2010;55(6):401-409.
[12]Dorocka-Bobkowska B,Medynski D,Prylinski M.Recent Advances in Tissue Conditioners for Prosthetic Treatment.Areview.Adv Clin Exp Med.2017;26(4):723-728.
[13]Craig RG,Gibbons P.Properties of resilient denture liners.JAm Dent Assoc.1961;63:382-390.
[14]辛振祥,张殿荣.现代橡胶配方设计[M].北京:化学工业出版社,2001.
[15]Altinci P,Mutluay M,S?derling E,et al.Antimicrobial efficacy and mechanical properties of BAC-modified hard and soft denture liners.Odontology.2017;106(1):1-7.
[16]De CF,Ayres M,Feltrin GP,et al.Effect of surface characteristics of soft liners and tissue conditioners and saliva on the adhesion and biofilm formation.Am J Dent.2018;31(1):45-52.
[17]Radi IA,Elmahrouky N.Effect of two different soft liners and thicknesses mediating stress transfer for immediately loaded2-implant supported mandibular overdentures:A finite element analysis study.J Prosthet Dent.2016;116(3):356-361.
[18]Sudhapalli S,Sudhapalli S.Time Dependent Effect of a Denture Cleanser on the Sorption and Solubility of Four Soft Liners-An Invitro Study.J Clin Diagn Res.2016;10(4):ZC100-103.
[19]Mello LJ,Gomes MJ,Juliana H,et al.Porosity of temporary denture soft liners containing antifungal agents.J Appl Oral Sci.2016;24(5):453-461.
[20]Mancuso DN,Goiato MC,Moreno A,et al.Effect of thermocycling on hardness,absorption,solubility and colour change of soft liners.Gerodontology.2012;29(2):e215-e219.
[21]Kostoulas I,Polyzois G,Mitsoudis A,et al.The effect of accelerated ageing on colour stability of visible light-cured(VLC)chairside denture liners.Gerodontology.2012;29(2):e239-e245.