脂滴包被蛋白基因对猪肌肉发育的影响
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摘要
PLIN(perilipin,脂滴包被蛋白)是脂滴表面含量最多的一种蛋白,被认为是影响猪生长、胴体及肉质性状的重要候选基因。PLIN基因几乎在所有组织中均有广泛表达,对脂肪组织中甘油三酯的分解和储存具有重要的双重调控作用,主要调控类固醇激素合成前体、胆固醇酯的分解;通过调节脂肪酶的功能和脂肪沉积来影响肌肉的生长发育并能影响高氧化能力组织内的线粒体功能。本文综述了猪PLIN基因背景、基因结构及组织分布情况,并对近年来PLIN基因对猪肌肉发育影响的研究进行了总结,解析其在骨骼肌发育差异调控网络中的地位和作用,为家猪生产性状改良提供理论依据。
Perilipin(PLIN) is the most abundant protein on the surface of lipid droplets, which is considered to be an important candidate gene for growth, carcass and meat quality. The PLIN gene is widely expressed in almost all tissues, and has an important dual regulation effect on the decomposition and storage of triglycerides in adipose tissue, mainly regulating the decomposition of steroid hormone synthesis precursors and cholesterol esters; it is not only can affects muscle growth and development by regulating the function of lipases and fat deposition but also can affect mitochondrial function in tissues with high oxidative capacity. This paper summarized the background, gene structure and tissue distribution of pig PLIN gene, and generalized the research about the influence of PLIN gene on pig muscle development in recent years. In this paper, the research progress of PLIN gene in recent years was summarized, whose position and role in the regulation network of skeletal muscle development were analyzed, which provided a theoretical basis for the improvement of production characteristics of domestic pigs.
Perilipin(PLIN) is the most abundant protein on the surface of lipid droplets, which is considered to be an important candidate gene for growth, carcass and meat quality. The PLIN gene is widely expressed in almost all tissues, and has an important dual regulation effect on the decomposition and storage of triglycerides in adipose tissue, mainly regulating the decomposition of steroid hormone synthesis precursors and cholesterol esters; it is not only can affects muscle growth and development by regulating the function of lipases and fat deposition but also can affect mitochondrial function in tissues with high oxidative capacity. This paper summarized the background, gene structure and tissue distribution of pig PLIN gene, and generalized the research about the influence of PLIN gene on pig muscle development in recent years. In this paper, the research progress of PLIN gene in recent years was summarized, whose position and role in the regulation network of skeletal muscle development were analyzed, which provided a theoretical basis for the improvement of production characteristics of domestic pigs.
引文
[1] Hashani M,Witzel H R,Pawella L M,et al.Widespread expression of perilipin 5 in normal human tissues and in diseases is restricted to distinct lipid droplet subpopulations[J].Cell Tissue Res,2018,374 (1):1-16.
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[5] Davoli R,Gandolfi G,Braglia S,et al.New SNP of the porcine perilipin 2 (PLIN2) gene,association with carcass traits and expression analysis in skeletal muscle[J].Mol Biol Rep,2011,38(3):1575-1583.
[6] Liu G,Jennen D G,Tholen E,et al.A genome scan reveals QTL for growth,fatness,leanness and meat quality in a Duroc-Pietrain resource population[J].Anim Genet,2007,38(3):241-252.
[7] Thomsen H,Lee H K,Rothschild M F,et al.Characterization of quantitative trait loci for growth and meat quality in a cross between commercial breeds of swine [J].J Anim Sci 82:2213-2228.
[8] Beeckmann P,Scho¨ffel J,Moser G,et al.Linkage and QTL mapping for Sus scrofa chromosome 1[J].J Anim Breed Genet 120:1–10.
[9] Sztalryd C and Brasaemle D L.Thematic review series:adipocyte biology.The perilipin family of structural lipid droplet proteins:stabilization of lipid droplets and control of lipolysis[J].J Lipid Res,2007,48(12):2547-2559.
[10] Zappaterra M,Mazzoni M,Zambonelli P,et al.Investigation of the Perilipin 5 gene expression and association study of its sequence polymorphism with meat and carcass quality traits in different pig breeds[J].Animal,2018,12(6):1135-1143.
[11] Gol S,Ros-Freixedes R,Zambonelli P,et al.Relationship between perilipin genes polymorphisms and growth,carcass and meat quality traits in pigs[J].Anim Breed Genet,2016,133(1):24-30.
[12] Dodson M V,Wei S,Duarte M,et al.Cell supermarket:adipose tissue as a source of stem cells[J].J Genomics,2013,1:39-44.
[13] Ramos S V,Turnbull P C,MacPherson R E K,et al.Changes in mitochondrial perilipin 3 and perilipin 5 protein content in rat skeletal muscle following endurance training and acute stimulated contraction[J].Exp Physiol,2015,100(4):450-462.
[14] Ramos S V,MacPherson R E K,Turnbull P C,et al.Higher PLIN5 but not PLIN3 content in isolated skeletal muscle mitochondria following acute in vivo contraction in rat hindlimb[J].Physiol Rep,2014,2(10):1-12.
[15] Yamamoto K,Miyoshi H,Cho K Y,et al.The data of change in macrophage gene expression which induced by perilipin 1 overexpression[J].Data Brief,2018,19:179-182.
[2] Londos C,Sztalryd C,Tansey J T,et al.Role of PAT proteins in lipid metabolism[J].Biochimie,2005,87(1):45-49.
[3] Kimmel A R,Brasaemle D L,Mcandrewshil M,et al.Adoption of PERILIPIN as a unifying nomenclature for the mammalian PAT-family of intracellular lipid storage droplet proteins[J].J Lipid Res,2010,51(3):468-471.
[4] Sztalryd C and Kimmel A R.Perilipins:lipid droplet coat proteins adapted for tissue-specific energy storage and utilization,and lipid cytoprotection[J].Biochimie,2014,96:96-101.
[5] Davoli R,Gandolfi G,Braglia S,et al.New SNP of the porcine perilipin 2 (PLIN2) gene,association with carcass traits and expression analysis in skeletal muscle[J].Mol Biol Rep,2011,38(3):1575-1583.
[6] Liu G,Jennen D G,Tholen E,et al.A genome scan reveals QTL for growth,fatness,leanness and meat quality in a Duroc-Pietrain resource population[J].Anim Genet,2007,38(3):241-252.
[7] Thomsen H,Lee H K,Rothschild M F,et al.Characterization of quantitative trait loci for growth and meat quality in a cross between commercial breeds of swine [J].J Anim Sci 82:2213-2228.
[8] Beeckmann P,Scho¨ffel J,Moser G,et al.Linkage and QTL mapping for Sus scrofa chromosome 1[J].J Anim Breed Genet 120:1–10.
[9] Sztalryd C and Brasaemle D L.Thematic review series:adipocyte biology.The perilipin family of structural lipid droplet proteins:stabilization of lipid droplets and control of lipolysis[J].J Lipid Res,2007,48(12):2547-2559.
[10] Zappaterra M,Mazzoni M,Zambonelli P,et al.Investigation of the Perilipin 5 gene expression and association study of its sequence polymorphism with meat and carcass quality traits in different pig breeds[J].Animal,2018,12(6):1135-1143.
[11] Gol S,Ros-Freixedes R,Zambonelli P,et al.Relationship between perilipin genes polymorphisms and growth,carcass and meat quality traits in pigs[J].Anim Breed Genet,2016,133(1):24-30.
[12] Dodson M V,Wei S,Duarte M,et al.Cell supermarket:adipose tissue as a source of stem cells[J].J Genomics,2013,1:39-44.
[13] Ramos S V,Turnbull P C,MacPherson R E K,et al.Changes in mitochondrial perilipin 3 and perilipin 5 protein content in rat skeletal muscle following endurance training and acute stimulated contraction[J].Exp Physiol,2015,100(4):450-462.
[14] Ramos S V,MacPherson R E K,Turnbull P C,et al.Higher PLIN5 but not PLIN3 content in isolated skeletal muscle mitochondria following acute in vivo contraction in rat hindlimb[J].Physiol Rep,2014,2(10):1-12.
[15] Yamamoto K,Miyoshi H,Cho K Y,et al.The data of change in macrophage gene expression which induced by perilipin 1 overexpression[J].Data Brief,2018,19:179-182.