HPLC法测定载5-取代-1-氮杂蒽醌类衍生物氧化石墨纳米粒中的药物含量
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摘要
目的:建立氧化石墨纳米粒中氮杂蒽醌类生物碱A1化合物含量测定的HPLC方法。方法:色谱柱为Inertsil ODS-3柱(250 mm×4. 6 mm,5μm);以甲醇-水-三乙胺(65∶35∶0. 05)为流动相;流速为1. 0 ml·min~(-1);检测波长为243 nm;柱温为30℃;进样体积为20μl。结果:A1的质量浓度在1. 00～100. 00μg·ml~(-1)线性关系良好(r=1. 000 0),平均加样回收率为102. 8%(RSD=1. 79%,n=9)。结论:该含量测定方法准确可靠、简单易行,可用于氧化石墨纳米粒中A1化合物的含量测定。
Objective: To establish an HPLC method for the determination of alkaloids in 5-substituted 1-alkaloids with azaanthraquinone skeleton (A1)-loaded graphite oxide nanoparticles. Methods: An Inertsil ODS-3 column (250 mm × 4. 6 mm,5 μm) was used with methanol-water-triethylamine (65 ∶ 35 ∶ 0. 05) as the mobile phase at the flow rate of 1. 0 ml·min~(-1). The A1 was detected at 243 nm. The column temperature was 30℃,and the injection volume was 20 μl. Results: The linear range of A1 was 1. 00-100. 00μg·ml~(-1) (r = 1. 000 0). The average recovery was 102. 8% (RSD = 1. 79%,n = 9). Conclusion: The method is accurate,reliable and simple,and can be used for the determination of A1 compound in graphite oxide nanoparticles.
Objective: To establish an HPLC method for the determination of alkaloids in 5-substituted 1-alkaloids with azaanthraquinone skeleton (A1)-loaded graphite oxide nanoparticles. Methods: An Inertsil ODS-3 column (250 mm × 4. 6 mm,5 μm) was used with methanol-water-triethylamine (65 ∶ 35 ∶ 0. 05) as the mobile phase at the flow rate of 1. 0 ml·min~(-1). The A1 was detected at 243 nm. The column temperature was 30℃,and the injection volume was 20 μl. Results: The linear range of A1 was 1. 00-100. 00μg·ml~(-1) (r = 1. 000 0). The average recovery was 102. 8% (RSD = 1. 79%,n = 9). Conclusion: The method is accurate,reliable and simple,and can be used for the determination of A1 compound in graphite oxide nanoparticles.
引文
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12 Liu Z,Robinson JT,Sun X,et al.PEGylated Nano-Graphene Oxide for Delivery of Water Insoluble Cancer Drugs[J].J Am Chem Soc,2008,130(33):10876-10877
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2马娜新.非共价功能化修饰的氧化石墨烯作为抗癌药物载体的研究[D].济南:山东大学硕士学位论文,2017
3龚水水,光善仪,柯福佑,等.红外光谱法氧化石墨烯羧基官能团含量的测定[J].中国测试,2016,42(4):38-44
4 Muthoosamy K,Abubakar IB,Bai RG,et al.Exceedingly Higher co-loading of Curcumin and Paclitaxel onto Polymer-functionalized Reduced Graphene Oxide for Highly Potent Synergistic Anticancer Treatment[J].Sci Rep,2016,6:32808
5 Thapa RK,Byeon JH,Ku SK,et al.Easy on-demand self-assembly of lateral nanodimensional hybrid graphene oxide flakes for near-infrared-induced chemothermal therapy[J].Npg Asia Materials,2017,9(8):e416
6 Wu SY,An SS,Hulme J.Current applications of graphene oxide in nanomedicine[J].Int J Nanomedicine,2015,10:9-24
7 Hung AH,Holbrook RJ,Rotz MW,et al.Graphene oxide enhances cellular delivery of hydrophilic small molecules by co-incubation[J].Acs Nano,2014,8(10):10168
8邢欢欢.5-取代-1-氮杂蒽醌类衍生物A7对氧化应激诱导的AD细胞毒性的抑制作用及其机制[D].广西桂林:桂林医学院硕士学位论文,2017
9胡观明.蒽醌类化合物的合成及对肿瘤细胞的细胞毒活性的研究[D].兰州:兰州大学硕士学位论文,2014
10陈芊,楚英豪.Hummers法制备氧化石墨烯[J].四川化工,2016,19(2):14-16
11史士强,纪宏宇,唐景玲,等.HPLC法测定载多柔比星氧化石墨纳米粒中的药物含量[J].中国药师,2013,16(3):334-336
12 Liu Z,Robinson JT,Sun X,et al.PEGylated Nano-Graphene Oxide for Delivery of Water Insoluble Cancer Drugs[J].J Am Chem Soc,2008,130(33):10876-10877
13 Huang P,Wang S,Wang X,et al.Surface Functionalization of Chemically Reduced Graphene Oxide for Targeted Photodynamic Therapy[J].J Biomed Nanotechnol,2015,11(1):117-125