冬季河流中抗生素抗性基因和微生物群落相关性研究
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摘要
为分析冬季河流中tetA、tetB、sul1和sul2抗性基因的分布特征与微生物群落的相关性,用Miseq高通量测序分析和荧光定量PCR技术进行。冬季河流地表水中抗性基因丰度比沉积物中的高2个数量级,磺胺类抗性基因丰度比四环素类的高1个数量级。4种抗性基因总丰度变化范围为3.65×10~2～7.94×10~7copies/L。河流中Proteobacteria和Bacteroidetes为绝对优势菌群,河流地表水与沉积物中优势菌群略有不同。4种抗性基因对样本的影响较大,微生物群落与抗性基因之间相关性较大。4种抗性基因与河流地表水样本均呈正相关,沉积物中仅有sul1与WS1、QS1呈正相关,其他均呈负相关。抗性基因的传播仅与特定的菌群有关,大多数菌属是影响抗性基因分布丰度的主要因素,且对四环素类抗性基因影响的菌群较多。
The distribution of tetA, tetB, sul1 and sul2 resistance genes in winter rivers was correlated with microbial community by high-throughput sequencing and real-time PCR. The abundance of resistance genes in surface waters of winter rivers is two orders of magnitude higher than that in sediments, and the abundance of sulfonamide resistance genes is one order of magnitude higher than that of tetracyclines. The total abundance of the four resistance genes varied from 3.65×10~2-7.94×10~7 Copies/L. Proteobacteria and Bacteroidetes in the river are absolute dominant bacteria, and the dominant species in the river surface water and sediment are slightly different. The four resistance genes have a greater impact on the sample, and the correlation between the microbial community and the resistance gene is greater. The four resistance genes were positively correlated with river surface water samples. Only sul1 was positively correlated with WS1 and QS1, and the others were negatively correlated. The spread of resistance genes is only related to a specific flora, and most of them are the main factors affecting the abundance of resistance genes, and there are more bacteria affecting the tetracycline resistance genes.
The distribution of tetA, tetB, sul1 and sul2 resistance genes in winter rivers was correlated with microbial community by high-throughput sequencing and real-time PCR. The abundance of resistance genes in surface waters of winter rivers is two orders of magnitude higher than that in sediments, and the abundance of sulfonamide resistance genes is one order of magnitude higher than that of tetracyclines. The total abundance of the four resistance genes varied from 3.65×10~2-7.94×10~7 Copies/L. Proteobacteria and Bacteroidetes in the river are absolute dominant bacteria, and the dominant species in the river surface water and sediment are slightly different. The four resistance genes have a greater impact on the sample, and the correlation between the microbial community and the resistance gene is greater. The four resistance genes were positively correlated with river surface water samples. Only sul1 was positively correlated with WS1 and QS1, and the others were negatively correlated. The spread of resistance genes is only related to a specific flora, and most of them are the main factors affecting the abundance of resistance genes, and there are more bacteria affecting the tetracycline resistance genes.
引文
[1]Laxminarayan R,Duse A,Wattal C,et al.Antibiotic resistance-the need for global solutions[J].The Lancet Infectious Diseases,2013,13(12):1057-1098.
[2]Lekunberri I,Villagrasa M,Balcázar,et al.Contribution of bacteriophage and plasmid DNA to the mobilization of antibiotic resistance genes in a river receiving treated wastewater discharges[J].Science of the Total Environment,2017,601/602:206-209.
[3]Qian X,Gu J,Sun W,et al.Diversity abundance and persistence of antibiotic resistance genes in various types of animal manure following industrial composting[J].Journal of Hazardous Materials,2018,344:716-722.
[4]Pruden A,Arabi M,Heather N,et al.Correlation between upstream human activities and riverine antibiotic resistance genes[J].Environmental Science and Technology,2012,46:11541-11549.
[5]Hsu J,Chen C,Young C,et al.Prevalence of sulfonamideresistant bacteria,resistance genes and integron-associated horizontal gene transfer in natural water bodies and soils adjacent to a swine feedlot in northern Taiwan[J].Hazard Mater,2014,277:34-43.
[6]Liao K,Bai Y,Huo Y,et al.Integrating microbial biomass composition and function to discern the level of anthropogenic activity in a river ecosystem[J].Environment International,2018,116:147-155.
[7]Su J,An X,Li B,et al.Metagenomics of urban sewage identifies an extensively shared antibiotic resistome in China[J].Microbiome,2017,5(1):84.
[8]Lin L,Yuan K,Liang X,et al.Occurrences and distribution of sulfonamide and tetracycline resistance genes in the Yangtze River Estuary and nearby coastal area[J].Marine Pollution Bulletin,2015,100(1):304-310.
[9]Bhaskar R,Suresh K D.River Ganges water as reservoir of microbes with antibiotic and metal ion resistance genes:high throughput metagenomic approach[J].Environmental Pollution,2019,246:443-451.
[10]Chen Y,Su J Q,Zhang J,et al.High-throughput profiling of antibiotic resistance gene dynamic in a drinking water riverreservoir system[J].Water Research,2019,149:179.
[11]Chen H,Bai X,Jing L,et al.Characterization of antibiotic resistance genes in the sediments of an urban river revealed by comparative metagenomics analysis[J].Science of the Total Environment,2019,653:1513-1521.
[12]Stoll C,Sidhu J P S,Tiehm A,et al.Prevalence of clinically relevant antibiotic resistance genes in surface water samples collected from Germany and Australia[J].Environmental Science and Technology,2012,46(17):9716-9726.
[13]徐艳,张远,郭昌胜,等.石家庄汪洋沟地区抗生素、抗性细菌和抗性基因污染特征[J].农业环境科学学报,2014,33(6):1174-1182.Xu Yan,Zhang Yuan,Guo Changsheng,et al.Pollution characteristics of antibiotics,resistant bacteria and resistance genes in Wangyanggou area,Shijiazhuang[J].Journal of Agricultural Environmental Science,2014,33(6):1174-1182.
[14]张毓森,叶军,苏建强.农田生态系统抗生素抗性研究进展与挑战[J].浙江大学学报:农业与生命科学版,2017,43(6):691-699.Zhang Yusen,Ye Jun,Su Jianqiang.Antibiotic resistance in agroecosystem:progress and challenges[J].Journal of Zhejiang University:Agriculture&Life Science,2017,43(6):691-699.
[15]Wang R,Zhang Y,Cao Z,et al.Occurrence of super antibiotic resistance genes in the downstream of the Yangtze River in China:prevalence and antibiotic resistance profiles[J].Science of the Total Environment,2019,651:1946-1957.
[16]Chen H,Zhang M.Effects of advanced treatment systems on the removal of antibiotic resistance genes in wastewater treatment plants from Hangzhou,China[J].Environmental Science and Technology,2013,47(15):8157-8163.
[17]Liu L,Liu C,Zheng J,et al.Elimination of veterinary antibiotics and antibiotic resistance genes from swine wastewater in the vertical flow constructed wetlands[J].Chemosphere,2013,91(8):1088-1093.
[18]张丹丹,郭亚平,任红云,等.福建省敖江下游抗生素抗性基因分布特征[J].环境科学,2018,39(6):2601-2606.Zhang Dandan,Guo Yaping,Ren Hongyun,et al.Characteristics of antibiotic resistance genes in downstream areas of Aojiang River,Fujian Province[J].Environmental Science,2018,39(6):2601-2606.
[19]曾涛涛,蒋小梅,韩科昌,等.生活污水处理厂微生物群落结构解析[J].安全与环境学报,2018,18(2):697-703.Zeng Taotao,Jiang Xiaomei,Han Kechang,et al.Analysis of microbial community structure in domestic sewage treatment plant[J].Journal of Safety and Environment,2018,18(2):697-703.
[20]侯佳林,聂唱,Perumal V,等.基于宏基因组学重建来自深海热液喷口的Epsilonproteobacteria基因组及其代谢分析[J].微生物学通报,2018,45(9):1843-1852.Hou Jialin,Nie Chang,Perumal V,et al.Metabolic analysis of Epsilonproteobacteria genomes reconstructed from the deep sea hydrothermal vent chimney based on metagenomic technology[J].Microbiology China,2018,45(9):1843-1852.
[21]Yang Y,Li B,Zou S,et al.Fate of antibiotic resistance genes in sewage treatment plant revealed by metagenomic approach[J].Water Research,2014,62:97-106.
[22]Teng Z,Zhu Y,Li M,et al.Microbial community composition and activity controls phosphorus transformation in rhizosphere soils of the Yeyahu Wetland in Beijing,China[J].The Science of the Total Environment,2018,628/629:1266-1277.
[23]袁立霞,罗晓,张文丽,等.制药废水厂微生物群落和多种抗性基因相关性分析[J].河北科技大学学报,2019,13(3):1-7.Yuan Lixia,Luo Xiao,Zhang Wenli,et al.Correlation between antibiotic resistance genes and microbial communities in pharmaceutical wastewater[J].Journal of Hebei University of Science and Technology,2019,13(3):1-7.
[2]Lekunberri I,Villagrasa M,Balcázar,et al.Contribution of bacteriophage and plasmid DNA to the mobilization of antibiotic resistance genes in a river receiving treated wastewater discharges[J].Science of the Total Environment,2017,601/602:206-209.
[3]Qian X,Gu J,Sun W,et al.Diversity abundance and persistence of antibiotic resistance genes in various types of animal manure following industrial composting[J].Journal of Hazardous Materials,2018,344:716-722.
[4]Pruden A,Arabi M,Heather N,et al.Correlation between upstream human activities and riverine antibiotic resistance genes[J].Environmental Science and Technology,2012,46:11541-11549.
[5]Hsu J,Chen C,Young C,et al.Prevalence of sulfonamideresistant bacteria,resistance genes and integron-associated horizontal gene transfer in natural water bodies and soils adjacent to a swine feedlot in northern Taiwan[J].Hazard Mater,2014,277:34-43.
[6]Liao K,Bai Y,Huo Y,et al.Integrating microbial biomass composition and function to discern the level of anthropogenic activity in a river ecosystem[J].Environment International,2018,116:147-155.
[7]Su J,An X,Li B,et al.Metagenomics of urban sewage identifies an extensively shared antibiotic resistome in China[J].Microbiome,2017,5(1):84.
[8]Lin L,Yuan K,Liang X,et al.Occurrences and distribution of sulfonamide and tetracycline resistance genes in the Yangtze River Estuary and nearby coastal area[J].Marine Pollution Bulletin,2015,100(1):304-310.
[9]Bhaskar R,Suresh K D.River Ganges water as reservoir of microbes with antibiotic and metal ion resistance genes:high throughput metagenomic approach[J].Environmental Pollution,2019,246:443-451.
[10]Chen Y,Su J Q,Zhang J,et al.High-throughput profiling of antibiotic resistance gene dynamic in a drinking water riverreservoir system[J].Water Research,2019,149:179.
[11]Chen H,Bai X,Jing L,et al.Characterization of antibiotic resistance genes in the sediments of an urban river revealed by comparative metagenomics analysis[J].Science of the Total Environment,2019,653:1513-1521.
[12]Stoll C,Sidhu J P S,Tiehm A,et al.Prevalence of clinically relevant antibiotic resistance genes in surface water samples collected from Germany and Australia[J].Environmental Science and Technology,2012,46(17):9716-9726.
[13]徐艳,张远,郭昌胜,等.石家庄汪洋沟地区抗生素、抗性细菌和抗性基因污染特征[J].农业环境科学学报,2014,33(6):1174-1182.Xu Yan,Zhang Yuan,Guo Changsheng,et al.Pollution characteristics of antibiotics,resistant bacteria and resistance genes in Wangyanggou area,Shijiazhuang[J].Journal of Agricultural Environmental Science,2014,33(6):1174-1182.
[14]张毓森,叶军,苏建强.农田生态系统抗生素抗性研究进展与挑战[J].浙江大学学报:农业与生命科学版,2017,43(6):691-699.Zhang Yusen,Ye Jun,Su Jianqiang.Antibiotic resistance in agroecosystem:progress and challenges[J].Journal of Zhejiang University:Agriculture&Life Science,2017,43(6):691-699.
[15]Wang R,Zhang Y,Cao Z,et al.Occurrence of super antibiotic resistance genes in the downstream of the Yangtze River in China:prevalence and antibiotic resistance profiles[J].Science of the Total Environment,2019,651:1946-1957.
[16]Chen H,Zhang M.Effects of advanced treatment systems on the removal of antibiotic resistance genes in wastewater treatment plants from Hangzhou,China[J].Environmental Science and Technology,2013,47(15):8157-8163.
[17]Liu L,Liu C,Zheng J,et al.Elimination of veterinary antibiotics and antibiotic resistance genes from swine wastewater in the vertical flow constructed wetlands[J].Chemosphere,2013,91(8):1088-1093.
[18]张丹丹,郭亚平,任红云,等.福建省敖江下游抗生素抗性基因分布特征[J].环境科学,2018,39(6):2601-2606.Zhang Dandan,Guo Yaping,Ren Hongyun,et al.Characteristics of antibiotic resistance genes in downstream areas of Aojiang River,Fujian Province[J].Environmental Science,2018,39(6):2601-2606.
[19]曾涛涛,蒋小梅,韩科昌,等.生活污水处理厂微生物群落结构解析[J].安全与环境学报,2018,18(2):697-703.Zeng Taotao,Jiang Xiaomei,Han Kechang,et al.Analysis of microbial community structure in domestic sewage treatment plant[J].Journal of Safety and Environment,2018,18(2):697-703.
[20]侯佳林,聂唱,Perumal V,等.基于宏基因组学重建来自深海热液喷口的Epsilonproteobacteria基因组及其代谢分析[J].微生物学通报,2018,45(9):1843-1852.Hou Jialin,Nie Chang,Perumal V,et al.Metabolic analysis of Epsilonproteobacteria genomes reconstructed from the deep sea hydrothermal vent chimney based on metagenomic technology[J].Microbiology China,2018,45(9):1843-1852.
[21]Yang Y,Li B,Zou S,et al.Fate of antibiotic resistance genes in sewage treatment plant revealed by metagenomic approach[J].Water Research,2014,62:97-106.
[22]Teng Z,Zhu Y,Li M,et al.Microbial community composition and activity controls phosphorus transformation in rhizosphere soils of the Yeyahu Wetland in Beijing,China[J].The Science of the Total Environment,2018,628/629:1266-1277.
[23]袁立霞,罗晓,张文丽,等.制药废水厂微生物群落和多种抗性基因相关性分析[J].河北科技大学学报,2019,13(3):1-7.Yuan Lixia,Luo Xiao,Zhang Wenli,et al.Correlation between antibiotic resistance genes and microbial communities in pharmaceutical wastewater[J].Journal of Hebei University of Science and Technology,2019,13(3):1-7.