长期负性情绪积累肝气郁结对轻度认知功能障碍患者端粒酶活性的影响
|
摘要
目的:探讨长期负性情绪积累肝气郁结与外周血单核细胞(PBMC)端粒酶活性的关系及在轻度认知功能障碍(MCI)形成过程中的影响。方法:采用问卷调查法筛选40～65岁肝气郁结型MCI患者,按照同性别、同年龄段、相同文化程度进行1∶1匹配;采用蒙特利尔认知评估量表(MoCA)等评估两组认知功能,采用艾森克人格问卷(EPQ)N维度量表评估两组被试情绪稳定性倾向,采用酶联免疫吸附法检测两组被试的PBMC端粒酶活性。结果:与正常组比较,MCI组MoCA量表总分及视空间与执行、注意、语言、抽象、延迟回忆得分均显著降低(P<0.05),EPQ量表N维度得分显著升高(P<0.05),PBMC端粒酶活性显著降低(P<0.01)。结论:长期负性情绪肝气郁结可造成人体端粒酶活性降低,这可能是引发患者认知功能下降的机制之一。
Objective: To investigate the relationship between liver qi stagnation caused by long-term accumulation of negative emotion and telomerase activity in peripheral blood mononuclear cells(PBMC), and its effect on the formation of mild cognitive impairment(MCI). Methods: Questionnaire survey was used to screen 40 to 65-year-old MCI patients and normal people. The two groups were matched according to the same gender, the same age group and the same education level. The Montreal cognitive assessment(MoCA) scale was used to evaluate the cognitive function of the two groups while the N dimension of EPQ scale was used to evaluate the emotional stability tendency of the two groups. The telomerase activity of the PBMC of the two groups was tested by enzyme-linked immunosorbent assay. Results: Compared with the normal group, the MCI group got lower scores on The total MoCA table and visual space and execution, attention, language, abstraction, delayed recall(P<0.05),got higher scores on N dimension of EPQ scale(P<0.05), and lower telomerase activity(P<0.01). Conclusion: Liver qi stagnation caused by long-term accumulation of negative emotions can bring down the activity of telomerase, which may be one of the mechanisms that cause the cognitive decline in MCI patients.
Objective: To investigate the relationship between liver qi stagnation caused by long-term accumulation of negative emotion and telomerase activity in peripheral blood mononuclear cells(PBMC), and its effect on the formation of mild cognitive impairment(MCI). Methods: Questionnaire survey was used to screen 40 to 65-year-old MCI patients and normal people. The two groups were matched according to the same gender, the same age group and the same education level. The Montreal cognitive assessment(MoCA) scale was used to evaluate the cognitive function of the two groups while the N dimension of EPQ scale was used to evaluate the emotional stability tendency of the two groups. The telomerase activity of the PBMC of the two groups was tested by enzyme-linked immunosorbent assay. Results: Compared with the normal group, the MCI group got lower scores on The total MoCA table and visual space and execution, attention, language, abstraction, delayed recall(P<0.05),got higher scores on N dimension of EPQ scale(P<0.05), and lower telomerase activity(P<0.01). Conclusion: Liver qi stagnation caused by long-term accumulation of negative emotions can bring down the activity of telomerase, which may be one of the mechanisms that cause the cognitive decline in MCI patients.
引文
[1]Bozoki A,Giordani B,Heidebrink J L,et al.Mild cognitive impairments predict dementia innondemented elderly patients with memory loss.Arch Neurol,2001,58(3):411-416
[2]Wang C,Yu J T,Wang H F,et al.Non-pharmacological interventions for patients with mild cognitive impairment:A meta-analysis of randomized controlled trials of cognition-based and exercise interventions.Journal of Alzheimers Disease Jad,2014,42(2):663-678
[3]周莉,吴超然,刘俐,等.老年轻度认知功能障碍的危险因素.华西医学,2016,31(2):374-378
[4]Hanninen T,Hallikainen M,Vanhanen M,et al.Prevalence of mild cognitive impairment:A population-based study in elderly subjects.Neurobiology of Aging,2000,21(3):148-154
[5]马素亚,畅洪昇,郑俊超,等.锁阳乙酸乙酯提取物改善慢性应激认知功能障碍神经电生理长时程增强表现.中华中医药杂志,2017,32(10):4608-4610
[6]詹向红,李伟,赵君玫,等.慢性愤怒应激对大鼠衰老进程及其神经内分泌免疫机制的影响.中华中医药杂志,2010,25(1):111-113
[7]Yaffe K,Petersen R C,Lindquist K,et al.Subtype of mild cognitive impairment and progression to dementia and death.Dementia&Geriatric Cognitive Disorders,2006,22(4):312-319
[8]Epel E S,Blackburn E H,Lin J,et al.Accelerated telomere shortening in response to life stress.Proceedings of the National Academy of Science,2004,101(49):17312-17315
[9]Winblad B,Palmer K,Kivipelto M,et al.Mild cognitive impairmentbeyond controversies,towards a consensus:Report of the International Working Group on Mild Cognitive Impairment.J Intern Med,2004,256(3):240-246
[10]曾红兵.肝气郁结型心理应激人群药物及心理干预研究.广州:广州中医药大学,2006
[11]Fossel M.cell senescense in human aging:A review of the theroy.In Vivo,2000,14:29-34
[12]Zhou Q G,Hu Y,Wu D L,et al.Hippocampal telomerase is involved in the modulation of depressive behaviors.Journal of Neuroscience,2011,31(34):12258-12269
[13]Wei Y B,Backlund L,Wegener G,et al.Telomerase dysregulation in the hippocampus of a rat model of depression:Normalization by lithium.International Journal of Neuropsychopharmacology,2015,18(7):1-8
[14]杨靖,詹向红,孙晔,等.四君子汤对D-半乳糖衰老模型小鼠心、肝、脑组织MDA含量及端粒酶活性的影响.中国中西医结合杂志,2005,(6):531-533
[15]万向.金匮肾气丸对衰老模型小鼠端粒酶的影响.重庆:西南大学,2011:38
[16]Wolkowitz O M,Mellon S H,Lindqvist D,et al.PBMC telomerase activity,but not leukocyte telomere length,correlates with hippocampal volume in major depression.Psychiatry Research,2015,232(1):58-64
[17]杨莉莉,钱雅琴,任秀宝.T淋巴细胞端粒及端粒酶与肿瘤免疫.中国肿瘤生物治疗杂志,2015,22(1):116-119
[18]刘江红,许贤豪.老年性痴呆的免疫机制研究进展.中国神经免疫学和神经病学杂志,2004,11(2):111-114
[19]Kumagai S,Chen S.Effects of exercise and physical activity for the improvement of cognitive function.Japanese Journal of Cognitive Neuroscience,2015,17(3):141-143
[20]Deng W,Cheung S T,Tsao S W,et al.Telomerase activity and its association with psychological stress,mental disorders,lifestyle factors and interventions:A systematic review.Psychoneuroendocrinology,2016,64:150
[21]亓胜辉,余林,马建苓.人格特质对认知老化的影响及其机制.心理科学进展,2013,21(1):96-107
[22]詹向红,李伟,徐玮玮,等.慢性愤怒应激对衰老大鼠学习记忆能力的影响及其脂质过氧化机制.中国老年学杂志,2010,30(6):752-753
[23]刘胜利,詹向红,李伟,等.慢性愤怒应激对脑老化大鼠学习记忆及血浆单胺类神经递质的影响.中华中医药杂志,2013,28(11):3185-3187
[24]詹向红,刘胜利,李伟,等.慢性愤怒应激对脑老化大鼠学习记忆及海马神经元的影响.中国老年学杂志,2013,33(20):5038-5040
[25]王友杰,詹向红,闫国立,等.特质怒及怒的表达与阿尔茨海默病的关系.中国老年学杂志,2014,34(17):4725-4726
[2]Wang C,Yu J T,Wang H F,et al.Non-pharmacological interventions for patients with mild cognitive impairment:A meta-analysis of randomized controlled trials of cognition-based and exercise interventions.Journal of Alzheimers Disease Jad,2014,42(2):663-678
[3]周莉,吴超然,刘俐,等.老年轻度认知功能障碍的危险因素.华西医学,2016,31(2):374-378
[4]Hanninen T,Hallikainen M,Vanhanen M,et al.Prevalence of mild cognitive impairment:A population-based study in elderly subjects.Neurobiology of Aging,2000,21(3):148-154
[5]马素亚,畅洪昇,郑俊超,等.锁阳乙酸乙酯提取物改善慢性应激认知功能障碍神经电生理长时程增强表现.中华中医药杂志,2017,32(10):4608-4610
[6]詹向红,李伟,赵君玫,等.慢性愤怒应激对大鼠衰老进程及其神经内分泌免疫机制的影响.中华中医药杂志,2010,25(1):111-113
[7]Yaffe K,Petersen R C,Lindquist K,et al.Subtype of mild cognitive impairment and progression to dementia and death.Dementia&Geriatric Cognitive Disorders,2006,22(4):312-319
[8]Epel E S,Blackburn E H,Lin J,et al.Accelerated telomere shortening in response to life stress.Proceedings of the National Academy of Science,2004,101(49):17312-17315
[9]Winblad B,Palmer K,Kivipelto M,et al.Mild cognitive impairmentbeyond controversies,towards a consensus:Report of the International Working Group on Mild Cognitive Impairment.J Intern Med,2004,256(3):240-246
[10]曾红兵.肝气郁结型心理应激人群药物及心理干预研究.广州:广州中医药大学,2006
[11]Fossel M.cell senescense in human aging:A review of the theroy.In Vivo,2000,14:29-34
[12]Zhou Q G,Hu Y,Wu D L,et al.Hippocampal telomerase is involved in the modulation of depressive behaviors.Journal of Neuroscience,2011,31(34):12258-12269
[13]Wei Y B,Backlund L,Wegener G,et al.Telomerase dysregulation in the hippocampus of a rat model of depression:Normalization by lithium.International Journal of Neuropsychopharmacology,2015,18(7):1-8
[14]杨靖,詹向红,孙晔,等.四君子汤对D-半乳糖衰老模型小鼠心、肝、脑组织MDA含量及端粒酶活性的影响.中国中西医结合杂志,2005,(6):531-533
[15]万向.金匮肾气丸对衰老模型小鼠端粒酶的影响.重庆:西南大学,2011:38
[16]Wolkowitz O M,Mellon S H,Lindqvist D,et al.PBMC telomerase activity,but not leukocyte telomere length,correlates with hippocampal volume in major depression.Psychiatry Research,2015,232(1):58-64
[17]杨莉莉,钱雅琴,任秀宝.T淋巴细胞端粒及端粒酶与肿瘤免疫.中国肿瘤生物治疗杂志,2015,22(1):116-119
[18]刘江红,许贤豪.老年性痴呆的免疫机制研究进展.中国神经免疫学和神经病学杂志,2004,11(2):111-114
[19]Kumagai S,Chen S.Effects of exercise and physical activity for the improvement of cognitive function.Japanese Journal of Cognitive Neuroscience,2015,17(3):141-143
[20]Deng W,Cheung S T,Tsao S W,et al.Telomerase activity and its association with psychological stress,mental disorders,lifestyle factors and interventions:A systematic review.Psychoneuroendocrinology,2016,64:150
[21]亓胜辉,余林,马建苓.人格特质对认知老化的影响及其机制.心理科学进展,2013,21(1):96-107
[22]詹向红,李伟,徐玮玮,等.慢性愤怒应激对衰老大鼠学习记忆能力的影响及其脂质过氧化机制.中国老年学杂志,2010,30(6):752-753
[23]刘胜利,詹向红,李伟,等.慢性愤怒应激对脑老化大鼠学习记忆及血浆单胺类神经递质的影响.中华中医药杂志,2013,28(11):3185-3187
[24]詹向红,刘胜利,李伟,等.慢性愤怒应激对脑老化大鼠学习记忆及海马神经元的影响.中国老年学杂志,2013,33(20):5038-5040
[25]王友杰,詹向红,闫国立,等.特质怒及怒的表达与阿尔茨海默病的关系.中国老年学杂志,2014,34(17):4725-4726