碳青霉烯类耐药的大肠埃希菌毒力因子与耐药基因相关性研究
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摘要
目的分析碳青霉烯类耐药的大肠埃希菌(E.Coli)耐药基因型与所含的毒力因子的关系。方法 PCR检测E.Coli毒力因子和耐药基因;采用Fisher确切概率法对耐药基因和毒力因子的关系进行分析。结果 95.7%(22/23)菌株超广谱β-内酰胺酶(ESBL)基因阳性,43.5%(10/23)菌株检出胞外黏多糖相关基因,87.0%(20/23)菌株检出P菌毛相关基因。耐药基因CTX-M-15型比CTX-M-55型菌株携带毒力因子papGⅡ的概率低(P=0.049)。耐药基因NDM-1阳性菌株比NDM-5阳性菌株中毒力因子papGⅢ携带率高(P=0.047)。结论 ESBL阳性菌株中不同CTX-M、NDM亚型与毒力因子papGⅡ、papGⅢ具有一定的相关性,毒力因子的携带情况与E.Coli耐药情况可能有关。
Objective To investigate the correlation between the genotype of drug-resistant genes and the virulence factors in carbapenem-resistant Escherichia coli(E.Coli) strains. Methods The virulence factors and drug-resistant genes were detected by PCR. The relationship between drug resistance genes and virulence strains were positive for extended spectrum beta-lactamase(ESBLs) gene, 43.5%(10/23) positive for the extracellular polysaccharide-related genes, and 87.0%(20/23) positive for the P ?mbriae-related genes. The positive rate of virulence factors papG Ⅱ in the CTX-M-15 strain was signi?cantly lower compared with that in the CTX-M-55 strain(P = 0.049). The positive rate of virulence factor papG Ⅲ in the NDM-5 positive strain was considerably higher than that in NDM-1 positive strains(P = 0.047). Conclusions The genotypes of CTX-M and NDM among ESBLs positive strains were asssociated with virulence factors papG virulence factors might be associated with the drug resistance genes in E.Coli.
Objective To investigate the correlation between the genotype of drug-resistant genes and the virulence factors in carbapenem-resistant Escherichia coli(E.Coli) strains. Methods The virulence factors and drug-resistant genes were detected by PCR. The relationship between drug resistance genes and virulence strains were positive for extended spectrum beta-lactamase(ESBLs) gene, 43.5%(10/23) positive for the extracellular polysaccharide-related genes, and 87.0%(20/23) positive for the P ?mbriae-related genes. The positive rate of virulence factors papG Ⅱ in the CTX-M-15 strain was signi?cantly lower compared with that in the CTX-M-55 strain(P = 0.049). The positive rate of virulence factor papG Ⅲ in the NDM-5 positive strain was considerably higher than that in NDM-1 positive strains(P = 0.047). Conclusions The genotypes of CTX-M and NDM among ESBLs positive strains were asssociated with virulence factors papG virulence factors might be associated with the drug resistance genes in E.Coli.
引文
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[2]Clermont O,Bonacorsi S,Bingen E.Rapid and simple determination of the Escherichia coli phylogenetic group.Appl Environ Microbiol,2000,66(10):4555-4558.
[3]Ho PL,Lo WU,Lai EL,Chow KH,Yam WC.Escherichia coli O25b-ST131 is an important cause of antimicrobial-resistant infections in women with uncomplicated cystitis.J Antimicrob Chemother,2012,67(10):2534-2535
[4]Liu X,Liu H,Wang L,Peng Q,Li Y,Zhou H,Li Q.Molecular characterization of extended-spectrum beta-lactamase-producing multidrug resistant Escherichia coli from swine in Northwest China.Front Microbiol,2018,9:1756.
[5]Pitout JD,Laupland KB,Church DL,Menard ML,Johnson JR.Virulence factors of Escherichia coli isolates that produce CTX-M-type extended-spectrum beta-lactamases.Antimicrob Agents Chemother,2005,49(11):4667-4670.
[6]Ejrn?s K,Stegger M,Reisner A,Ferry S,Monsen T,Holm SE,Lundgren B,Frimodt-M?ller N.Characteristics of Escherichia coli causing persistence or relapse of urinary tract infections:phylogenetic groups,virulence factors and biofilm formation.Virulence,2011,2(6):528-537.
[7]Colom K,Pérez J,Alonso R,Fernández-Aranguiz A,Lari?o E,Cisterna R.Simple and reliable multiplex PCR assay for detection of blaTEM,blaSHV and blaOXA-1 genes in Enterobacteriaceae.FEMS Microbiol Lett,2003,223(2):147-151.
[8]Poirel L,Walsh TR,Cuvillier V,Nordmann P.Multiplex PCRfor detection of acquired carbapenemase genes.Diagn Microbiol Infect Dis,2011,70(1):119-123.
[9]Manges AR,Johnson JR,Foxman B,O’Bryan TT,Fullerton KE,Riley LW.Widespread distribution of urinary tract infections caused by a multidrug-resistant Escherichia coli clonal group.NEngl J Med,2001,345(14):1007-1013.
[10]Graveline R,Lavoie R,Garneau P,Daigle F,Sénéchal S,Martin C,Harel J.Monitoring F1651 P-like fimbria expression at the single-cell level reveals a highly heterogeneous phenotype.Infect Immun,2015,83(5):1929-1939.
[11]Barrios-Villa E,Cortés-Cortés G,Lozano-Zaraín P,Arenas-Hernández MMP,Martínez de la Pe?a CF,Martínez-Laguna Y,Torres C,RochaGracia RDC.Adherent/invasive Escherichia coli(AIEC)isolates from asymptomatic people:new E.coli ST131 O25:H4/H30-Rx virotypes.Ann Clin Microbiol Antimicrob,2018,17(1):42.