水热反应时间对三维石墨烯血液相容性的影响
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摘要
以石墨烯为原料,采用乙二胺水热还原的方法制备三维石墨烯,在实验中通过控制水热反应时间来调控所得到的三维石墨烯的孔径大小,并通过扫描电子显微镜(SEM)、X射线光电子能谱(XPS)对三维石墨烯的表面形貌、结构和成分进行了表征,利用蛋白电泳、血小板粘附实验、溶血实验对制备样品表面的蛋白吸附率、血小板粘附等情况进行了对比。随着水热反应时间的延长,三维石墨烯的孔径由340μm逐渐减小为230μm,且N元素被引入到材料中,形成了新的化学键。将水热反应时间由6h延长至24h,使得制备出的三维石墨烯样品表面对牛血清白蛋白(BSA)的吸附量由2.6%上升至16.8%,溶血率由2.2%降至0.3%;同时,抗血小板粘附的能力也在不断增强。水热反应时间的延长可以减小三维石墨烯材料的表面孔径、增加其比表面积,提高材料表面对BSA的吸附能力和抗血小板粘附的能力,同时不断降低材料溶血率。水热反应时间的延长有利于提高石墨烯材料的血液相容性。
Three-dimensional graphene were produced via the ethylenediamine hydrothermal reduction method by using the two-dimensional graphene.And in this experiment,the hole's diameter of three-dimensional graphene was adjusted by the hydrothermal reaction time.In order to characterize the surface topography,the chemical characteristics and components of three-dimensional graphene,scanning electron microscopy(SEM)and X-ray photoelectron spectroscopy(XPS)were used.The protein absorption rate and the platelet conglutination rate of three-dimensional graphene were also contrasted by protein electrophoresis test,platelet conglutination test and hemolysis test.The hole's diameter of three-dimensional graphene decreased from 340 to 230μm with the increasing of hydrothermal reaction time.The introduction of nitrogen into the three-dimensional graphene formed new chemical bonds.When the hydrothermal time was extended from 6 to 24 h,the bovine serum albumin absorption rate of three-dimensional graphene was improved from 2.6%to 16.8%and the hemolysis ratio was decreased from 2.2%to 0.3%.In addition,the capability of resist platelet conglutination of three-dimensional graphene was increased.With the increasing of hydrothermal reaction time,the hole's diameter of three-dimensional graphene was reduced and the specific surface area was increased.The ability of BSA absorption and the capability of resist platelet conglutination were also improved.Furthermore,the hemolysis ratios were decreased.Therefore,the increasing of hydrothermal reaction time was conducive to enhance the hemocompatibility of three-dimensional graphene.
Three-dimensional graphene were produced via the ethylenediamine hydrothermal reduction method by using the two-dimensional graphene.And in this experiment,the hole's diameter of three-dimensional graphene was adjusted by the hydrothermal reaction time.In order to characterize the surface topography,the chemical characteristics and components of three-dimensional graphene,scanning electron microscopy(SEM)and X-ray photoelectron spectroscopy(XPS)were used.The protein absorption rate and the platelet conglutination rate of three-dimensional graphene were also contrasted by protein electrophoresis test,platelet conglutination test and hemolysis test.The hole's diameter of three-dimensional graphene decreased from 340 to 230μm with the increasing of hydrothermal reaction time.The introduction of nitrogen into the three-dimensional graphene formed new chemical bonds.When the hydrothermal time was extended from 6 to 24 h,the bovine serum albumin absorption rate of three-dimensional graphene was improved from 2.6%to 16.8%and the hemolysis ratio was decreased from 2.2%to 0.3%.In addition,the capability of resist platelet conglutination of three-dimensional graphene was increased.With the increasing of hydrothermal reaction time,the hole's diameter of three-dimensional graphene was reduced and the specific surface area was increased.The ability of BSA absorption and the capability of resist platelet conglutination were also improved.Furthermore,the hemolysis ratios were decreased.Therefore,the increasing of hydrothermal reaction time was conducive to enhance the hemocompatibility of three-dimensional graphene.
引文
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[5] Yu D,Dai L.Self-assembled graphene carbon nanotube hybrid films for supercapacitors[J].Journal of Physical Chemistry Letters,2010,1(2):467-470.
[6] Li X,Cai W,An J,et al.Large-area synthesis of highquality and uniform graphene films on copper foils[J].Science,2009,324(5932):1312-1314.
[7] Huang Guojia,Chen Zhigang,Li Maodong,et al.Surface functional modification of graphene and graphene oxide[J].Acta Chimica Sinica,2016,74(10):789-799(in Chinese).黄国家,陈志刚,李茂东,等.石墨烯和氧化石墨烯的表面功能化改性[J].化学学报,2016,74(10):789-799.
[8] Li D J,Niu L.Effects of COOH+ion implantation on hemocompatibility of polypropylene[J].Sci China Ser E,2002,45(6):666-670.
[9] Xu Y,Sheng K,Li C,et al.Self-assembled graphene hydrogel via a one-step hydrothermal process[J].ACS Nano,2010,4(7):4324-4330.
[10] Chen Z P,Ren W C,Gao L B,et al.Three-dimensional flexible and conductive interconnected graphene networks grown by chemical vapour deposition[J].Nat mater,2011,10(6):424-428.
[11] Wu Tao,Zhang Xiaowei,Jiang Yehua.Growth mechanisms of graphene prepared by CVD on transition metals substrates[J].Journal of Functional Materials,2017,48(6):6050-6056(in Chinese).吴涛,张晓伟,蒋业华.过渡金属表面CVD石墨烯生长机理研究进展[J].功能材料,2017,48(6):6050-6056.
[12] Gao H,Duan H.2Dand 3Dgraphene materials:preparation and bioelectrochemical applications[J].Biosensors&Bioelectronics,2015,65:404-419.
[13] Tan Simin,Wang Shuaixing,Zhao Qing,et al.Effects of hydrothermal time on hydroxyapatite synthesis of microarc oxidized titanium[J].Surface Technology,2014,43(3):20-24(in Chinese).谭思民,王帅星,赵晴,等.水热时间对钛合金微弧氧化膜合成羟基磷灰石的影响[J].表面技术,2014,43(3):20-24.
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[16] Gao Jiacheng,Li Longchuan,Wang Yong,et al.Biocompatibility of magnesium after surface modification by heat-organic films-treatment[J].Rare Metal Materials and Engineering,2005,34(6):930-937(in Chinese).高家诚,李龙川,王勇,等.表面改性纯镁的细胞毒性和溶血率[J].稀有金属材料与工程,2005,34(6):930-937.
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