血肿瘤坏死因子-α、透明质酸在骨关节炎滑膜炎的特点
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摘要
目的探讨血清学指标肿瘤坏死因子-α(TNF-α)、透明质酸(HA)与膝骨关节炎(KOA)关节滑膜血流信号的相关性。方法将KOA患者根据高频能量多普勒超声下有无关节滑膜血流信号进行分组,采用ELISA试验进行检测KOA各组及健康人群对照组血清中血TNF-α、HA值。组间采用t检验或秩合检验,多组间比较采用单因素方差分析,两因素间相关性分析采用Spearman相关分析。结果①关节滑膜有血流信号组的年龄、WOMAC评分均高于关节滑膜无血流信号组,差异有统计学意义(F=0.082,1.862,均P<0.05),②在KOA关节滑膜血流信号组、KOA关节滑膜无血流信号组及正常对照组的之间,各组的血TNF-α、HA值不同,差异有统计学意义,均P<0.05,关节滑膜无血流信号组最高,正常对照组最低。结论血TNF-α、HA与关节滑膜血流信号相关,对膝骨关节炎滑膜炎的病情评估上有一定意义。
Objective To evaluate the correlation of serum levels of tumor necrosis factor-α(TNF-α), hyaluronic acid(HA) with synovial blood signals of knee osteoarthritis(KOA). Methods Patients with knee osteoarthritis were divided into two groups according to whether the synovial blood signal was checked by High-frequency energy Doppler ultrasound. The serum concentrations of TNF-αand HA were detected by ELISA kit. The t test or the rank test was used between the groups. One-way analysis of variance was used for comparison between groups. Spearman correlation analysis was used for correlation analysis between the two factors. Results ① The age and WOMAC scores were higher than those without the synovial blood signals. The difference was statistically significant(F=0.082,1.862, both P<0.05). ② The serum concentrations of TNF-α or HA were statistically significant among synovial blood signal group、without synovial blood signal group and normal group, all P<0.05, which without synovial blood signal group was the highest and the normal control group was the lowest. Conclusion Serums TNF-α and HA concentrations were related to synovial blood signals and have important value in the evaluation of knee osteoarthritis.
Objective To evaluate the correlation of serum levels of tumor necrosis factor-α(TNF-α), hyaluronic acid(HA) with synovial blood signals of knee osteoarthritis(KOA). Methods Patients with knee osteoarthritis were divided into two groups according to whether the synovial blood signal was checked by High-frequency energy Doppler ultrasound. The serum concentrations of TNF-αand HA were detected by ELISA kit. The t test or the rank test was used between the groups. One-way analysis of variance was used for comparison between groups. Spearman correlation analysis was used for correlation analysis between the two factors. Results ① The age and WOMAC scores were higher than those without the synovial blood signals. The difference was statistically significant(F=0.082,1.862, both P<0.05). ② The serum concentrations of TNF-α or HA were statistically significant among synovial blood signal group、without synovial blood signal group and normal group, all P<0.05, which without synovial blood signal group was the highest and the normal control group was the lowest. Conclusion Serums TNF-α and HA concentrations were related to synovial blood signals and have important value in the evaluation of knee osteoarthritis.
引文
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[2] Mary B. Goldring,Miguel Otero. Inflammation in osteoarthritis[J]. NIH Public Access Author Manuscript,2011,23(5):471-478.
[3] Nefla M,Holzinger D,Berenbaum F, et al, The danger from within:alarmins in arthritis[J]. Nat Rev Rheumatol,2016,12(11):669-683.
[4] Lopez-Armada MJ, Carames B, Lires-Dean M, et al. Cytokines, tumor necrosis factor-alpha and interleukin-1 beta, differentially regulate apoptosis in osteoarthritis cultured human chondrocytes[J]. Osteoarthritis and CartiIage,2006,14(7):660-669.
[5] Jing Ding, Xin Niu, Yan Su, et al. Expression of synovial fluid biomarkers in patients with knee osteoarthritis and meniscus injury[J]. EXPERIMENTAL AND THERAPEUTIC MEDICINE 2017,14:1609-1613.
[6]李敏,梁翼,吴晓惠,等.伴骨髓水肿的膝骨节炎患者白细胞介素-1β、白细胞介素-6、肿瘤坏死因子-α的相关性研究[J].中华风湿病学杂志,2012,16(2):107-110.
[7] Sasaki E, Tsuda E, Yamamoto Y, et al. Serum hyaluronic acid concentration predicts the progression of joint space narrowing in normal knees and established knee osteoarthritis-a five-year prospective cohort study[J].Arthritis Research&Therapy 2015,17:283-293.
[8] Sellam J, Berenbaum F. The role of synovitis in pathophysiology and clinical symptoms of osteoarthritis[J]. Nat Rev Rheumatol,2010,6:625-635.
[9]庄超,徐南伟,高翔,等.骨关节炎动物模型中血清透明质酸的变化及其意义田.中华风湿病学杂志,2013,17(12):833-835.