重庆自育水稻骨干亲本稻瘟病抗性评价及其抗病基因分布分析
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摘要
【目的】明确重庆自育水稻骨干亲本的稻瘟病抗性及其抗病基因分布情况,为高效选育抗稻瘟病水稻品种提供优质亲本。【方法】采用病圃自然诱发和人工接种相结合的方法,对重庆地区自育的73份水稻骨干亲本稻瘟病抗性进行鉴定,并利用Pi2、Pi5、Pi9、Pi-kh、Pib、Pita和Pi-km1 7个抗稻瘟病基因的分子标记对其基因型进行检测以分析抗病基因分布和聚合方式。【结果】73份水稻亲本中,田间稻瘟病抗性鉴定为高抗和抗病的亲本(42份)占57.5%,携带抗病基因的水稻亲本(49份)占67.1%,其中仅携带1个抗病基因的亲本有18份,表型高抗的有1份,表型抗病的有7份,抗病比率44.4%;聚合2个抗病基因的亲本有18份,表型高抗的有4份,表型抗病的有6份,抗病比率55.6%,聚合3个抗病基因的亲本有9份,均表型抗病,抗病比率100.0%;聚合4个抗病基因的亲本有4份,表型高抗的有2份,表型抗病的有2份,抗病比率100.0%;不携带检测基因的亲本(24份)占32.9%,其中表型高抗的有3份,表型抗病的有8份,抗病比率45.8%;抗病基因Pi2、Pi5、Pi-kh、Pib和Pita在携带抗病基因的水稻骨干亲本中分布频率不同,分别为13.7%、31.5%、31.5%、37.0%和19.2%;未检测出含抗病基因Pi9和Pi-km1的亲本,也未检测到同时携带抗病基因Pi2、Pi5、Pi-kh、Pib和Pita的亲本。不同水稻亲本携带抗病基因不同及聚合方式不同,抗病表现均存在差异。【结论】基因聚合可有效提高亲本的抗病性,但基因聚合后抗病比率并非简单地叠加,应从中筛选田间抗病较强的亲本作为抗稻瘟病育种的候选亲本。
【Objective】This study aimed to confirm resistance of self-bred rice backbone parents germplasm against rice blast in Chongqing area and the distribution of resistance genes,and provide parents foundation for efficient breeding of rice varieties resistant to rice blast.【Method】Seventy-three rice germplasms were evaluated on resistance against rice blast through combined methods of natural induction in disease field and artificial inoculation in a disease nursery. The molecular markers of seven resistance genes,including Pi2,Pi5,Pi9,Pi-kh,Pib,Pita and Pi-km1,were used to detect the distribution and polymerization patterns.【Result】The results showed that among the 73 rice parents,57.5%(42 parents)of the rice germplasms expressed high resistance(HR)and resistance(R)to rice blast in field. 67.1%(49 parents)of them carried resistance genes. Among them,there were eighteen parents carried only one resistance gene,with one expressed HR and seven expressed R,the resistance rate was 44.4%. Eighteen parents carried two resistance genes,four of them were HR,six of them were R,and the resistance rate was 55.6%. Nine carried three resistance genes,all of them were R,and the resistance rate was 100.0%. Only four carried four resistance genes,two of them were HR,two of them were R,and the resistance rate was 100.0%. 32.9%(24 parents)of rice parents carried no detected genes. Among them,three were HR,and eight were R,the resistance rate was 45.8%. The distribution frequency of resistance genes Pi2,Pi5,Pi-kh,Pib and Pita in rice backbone parents carrying resistance genes were 13.7%,31.5%,31.5%,37.0% and 19.2% respectively. Parents carrying Pi9 and Pi-km1 genes were not detected. And the parents carrying Pi2,Pi5,Pi-kh,Pib and Pita at the same time were not detected. Different tested parents carried various resistance genes and were with various polymerization patterns,and the resistance performance was also different.【Conclusion】Gene polymerization can increase the resistance of parents. But the resistance rate after gene polymerization is not a simple add-up. The parents with strong resistance in field should be selected to be candidate parents in rice blast resistance breeding.
【Objective】This study aimed to confirm resistance of self-bred rice backbone parents germplasm against rice blast in Chongqing area and the distribution of resistance genes,and provide parents foundation for efficient breeding of rice varieties resistant to rice blast.【Method】Seventy-three rice germplasms were evaluated on resistance against rice blast through combined methods of natural induction in disease field and artificial inoculation in a disease nursery. The molecular markers of seven resistance genes,including Pi2,Pi5,Pi9,Pi-kh,Pib,Pita and Pi-km1,were used to detect the distribution and polymerization patterns.【Result】The results showed that among the 73 rice parents,57.5%(42 parents)of the rice germplasms expressed high resistance(HR)and resistance(R)to rice blast in field. 67.1%(49 parents)of them carried resistance genes. Among them,there were eighteen parents carried only one resistance gene,with one expressed HR and seven expressed R,the resistance rate was 44.4%. Eighteen parents carried two resistance genes,four of them were HR,six of them were R,and the resistance rate was 55.6%. Nine carried three resistance genes,all of them were R,and the resistance rate was 100.0%. Only four carried four resistance genes,two of them were HR,two of them were R,and the resistance rate was 100.0%. 32.9%(24 parents)of rice parents carried no detected genes. Among them,three were HR,and eight were R,the resistance rate was 45.8%. The distribution frequency of resistance genes Pi2,Pi5,Pi-kh,Pib and Pita in rice backbone parents carrying resistance genes were 13.7%,31.5%,31.5%,37.0% and 19.2% respectively. Parents carrying Pi9 and Pi-km1 genes were not detected. And the parents carrying Pi2,Pi5,Pi-kh,Pib and Pita at the same time were not detected. Different tested parents carried various resistance genes and were with various polymerization patterns,and the resistance performance was also different.【Conclusion】Gene polymerization can increase the resistance of parents. But the resistance rate after gene polymerization is not a simple add-up. The parents with strong resistance in field should be selected to be candidate parents in rice blast resistance breeding.
引文
陈利锋,徐敬友.2005.农业植物病理[M].北京:中国农业出版社.[Chen L F,Xu J Y.2005.Agricultural Plant Pathology[M].Beijing:China Agriculture Press.]
付崇允,王玉平,马玉清,王霞玲,李仕贵.2006.丽江新团黑谷近等基因系抗稻瘟病分析[J].作物学报,32(6):799-804.[Fu C Y,Wang Y P,Ma Y Q,Wang X L,Li S G.2006.Identification of IRBL near-isogenic lines for rice blast resistance[J].Acta Agronomic Sinica,32(6):799-804.]
高利军,高汉亮,颜群,周萌,周维永,张晋,邓国富.2010.4个抗稻瘟病基因分子标记的建立及在水稻亲本中的分布[J].杂交水稻,25(S1):294-298.[Gao L J,Gao H L,Yan Q,Zhou M,Zhou W Y,Zhang J,Deng G F.2010.Establishment of markers for four blast genes and marker distribution in rice parents[J].Hybrid Rice,25(S1):294-298.]
管玉圣,欧阳杰,黄乾龙,郭爽,朱子超,熊英,王静.2017.重庆地区部分水稻亲本材料的抗稻瘟病基因检测分析[J].南方农业,11(34):17-19.[Guan Y S,Ouyang J,Huang QL,Guo S,Zhu Z C,Xiong Y,Wang J.2017.Detecting and analysis of rice blast resistance gene in some rice parents in Chongqing area[J].South China Agriculture,11(34):17-19.]
黄富,谢戎,刘成元,叶华智,杨文鈺.2007.亲本抗瘟性对杂交水稻组合抗瘟性的影响[J].杂交水稻,22(2):64-68.[Huang F,Xie R,Liu C Y,Ye H Z,Yang W Y.2007.Effects of blast resistance of parents on that of their F1hybrids in rice[J].Hybrids Rice,22(2):64-68.]
冷祯陆.2011.78份水稻制恢材料的抗瘟性、农艺性状及杂种优势评价[D].成都:四川农业大学.[Leng Z L.2011.Evaluation of resistance to rice blast,main agronomic traits and heterosis of 78 rice materials of breeding restorer line[D].Chengdu:Sichuan Agricultural University.]
李建修.2012.水稻制恢材料对稻曲病、稻瘟病抗性及农艺性状评价[D].成都:四川农业大学.[Li J X.2012.Evaluation of resistance to rice false smut and rice blast,and agronomic characters evaluation of rice materials of breeding restorer line[D].Chengdu:Sichuan Agricultural University.]
李进斌,王甜,许明辉.2012.云南地方稻种抗稻瘟病基因Pita和Pi-b的鉴定[J].中国水稻科学,26(5):593-599.[Li J B,Wang T,Xu M H.2012.Identification of Pi-ta and Pi-b genes for rice blast resistance of rice landraces from Yunnan Province[J].Chinese Journal of Rice Science,26(5):593-599.]
刘洋,徐培洲,张红宇,徐建第,吴发强,吴先军.2008.水稻抗稻瘟病Pib基因的分子标记辅助选择与应用[J].中国农业科学,41(1):9-14.[Liu Y,Xu P Z,Zhang H Y,Xu JD,Wu F Q,Wu X J.2008.Marker-assisted selection and application of blast resistant gene Pib in rice[J].Scientia Agricultura Sinica,41(1):9-14.]
宋广树,孙蕾,刘志全,陈寞军,朱秀侠,周广春,封云,浦桂君,侯立刚.2016.吉林省主要水稻品种稻瘟病抗性分析及分子辅助改良[J].西北农业学报,25(5):677-683.[Song G S,Sun L,Liu Z Q,Chen M J,Zhu X X,Zhou G C,Feng Y,Pu G J,Hou L G.2016.Rice blast resistance analysis and molecular assistant improvement of main rice varieties in Jilin Province[J].Acta Agriculturae Boreali-occidentalis Sinica,25(5):677-683.]
孙国昌,杜新法,陶荣祥,孙漱沅.1998.水稻稻瘟病防治策略和21世纪研究展望[J].植物病理学报,28(4):289-292.[Sun G C,Du X F,Tao R X,Sun S Y.1998.Control tactics and prospect of rice blast research in 21thcentury[J].Acta Phytopathology Sinica,28(4):289-292.]
王丽丽,赵家铭,马作斌,郑文静,马殿荣.2017.辽宁地区水稻资源抗稻瘟病基因的检测分析[J].植物遗传资源学报,18(2):325-339.[Wang L L,Zhao J M,Ma Z B,Zheng W J,Ma D R.2017.Identificating and analyzing to rice blast gene in rice germplasm resources of Liaoning Province[J].Journal of Plant Genetic Resource,18(2):325-339.]
杨秀娟,朱春雨,阮宏椿,杜宜新,关瑞峰,陈福如.2008.福建省稻瘟病菌毒性类型及部分水稻品种(组合)抗病性[J].福建农林大学学报(自然科学版),37(3):244-247.[Yang X J,Zhu C Y,Ruan H C,Du Y X,Guan R F,Chen F R.2008.Pathogenic type of Magnaporthe grisea Barr.and the resistance of some rice cultivars to the pathogens in Fujian Province[J].Journal of Fujian Agriculture and Forestry University(National Science Edition),37(3):244-247.]
张雪梅.2011.四川省水稻主栽品种抗瘟性评价和内恢99-14抗瘟遗传性分析[D].成都:四川农业大学.[Zhang X M.2011.Resistance evaluation of rice cultivar from Sichuan Province and genetic analysis of Neihui 99-14 to blast disease[D].Chengdu:Sichuan Agricultural University.]
张银霞,田蕾,李培富.2016.宁夏自育水稻品种及部分引进品种的稻瘟病抗性基因分子标记检测[J].西北农业学报,25(7):989-996.[Zhang Y X,Tian L,Li P F.2016.Detection and analysis of rice blast resistant gene in rice varieties bred in Ningxia and introduced from outside Ningxia[J].Acta Agriculturae Boreali-occidentalis Sinica,25(7):989-996.]
张羽,冯志峰,崔明珠,张晓娟.2013.稻瘟病抗性基因Pi-km在陕西省稻种资源中的分布状况[J].分子植物育种,11(3):311-316.[Zhang Y,Feng Z F,Cui M Z,Zhang X J.2013.Distribution of blast resistance gene Pi-km in rice germplasms in Shaanxi Province of northwest China[J].Molecular Plant Breeding,11(3):311-316.]
Dean R,Van Kan J A L,Pretorius Z A,Hammond-Kosack KE,Pietro A D,Spanu P D,Rudd J J,Dickman M,Kahmann R,Ellis J,Foster G D.2012.The top 10 fungal pathogens in molecular plant pathology[J].Molecular Plant Pathology,13(4):414-430.
Deng Y W,Zhai K R,Xie Z,Yang D Y,Zhu X D,Liu J Z,Wang X,Qin P,Yang Y Z,Zhang G M,Li Q,Zhang J F,Wu S Q,Milazzo J,Mao B Z,Wang E T,Xie H A,Tharreau D,He Z H.2017.Epigenetic regulation of antagonistic receptors confers rice blast resistance with yield balance[J].Science,355(6238):962-965.
Jia Y L,McAdams S A,Bryan G T,Hershey H P,Valent B.2000.Direct interaction of resistance gene and avirulence gene products confers rice blast resistance[J].The EM-BO Journal,19(5):4004-4014.
Jia Y L,Wang Z H,Singh P.2002.Development of dominant rice blast Pita resistance gene markers[J].Crop Science,42(6):2145-2149
Moytri R,Jia Y L,Richard D C.2012.Structure,function,and co-evolution of rice blast resistance genes[J].Acta Agronomica Sinica,8(38):381-393.
Ramkumar G,Srinivasarao K,Madhan Mohan K,Sudarshan I,Sivaranjani A K P,Gopalakrishna K,Neeraja C N,Balachandran S M,Sundaram R M,Prasad M S,Shobha Rani N,Rama Prasad A M,Viraktamath B C,Madhav MS.2011.Development and validation of functional marker targeting an InDel in the major rice blast disease resistance gene Pi54(Pi-kh)[J].Molecular Breeding,27(1):129-135.
Skamnioti P,Gurr S J.2009.Against the grain:safeguarding rice from rice blast disease[J].Trends in Biotechnology,27(3):141-150.
付崇允,王玉平,马玉清,王霞玲,李仕贵.2006.丽江新团黑谷近等基因系抗稻瘟病分析[J].作物学报,32(6):799-804.[Fu C Y,Wang Y P,Ma Y Q,Wang X L,Li S G.2006.Identification of IRBL near-isogenic lines for rice blast resistance[J].Acta Agronomic Sinica,32(6):799-804.]
高利军,高汉亮,颜群,周萌,周维永,张晋,邓国富.2010.4个抗稻瘟病基因分子标记的建立及在水稻亲本中的分布[J].杂交水稻,25(S1):294-298.[Gao L J,Gao H L,Yan Q,Zhou M,Zhou W Y,Zhang J,Deng G F.2010.Establishment of markers for four blast genes and marker distribution in rice parents[J].Hybrid Rice,25(S1):294-298.]
管玉圣,欧阳杰,黄乾龙,郭爽,朱子超,熊英,王静.2017.重庆地区部分水稻亲本材料的抗稻瘟病基因检测分析[J].南方农业,11(34):17-19.[Guan Y S,Ouyang J,Huang QL,Guo S,Zhu Z C,Xiong Y,Wang J.2017.Detecting and analysis of rice blast resistance gene in some rice parents in Chongqing area[J].South China Agriculture,11(34):17-19.]
黄富,谢戎,刘成元,叶华智,杨文鈺.2007.亲本抗瘟性对杂交水稻组合抗瘟性的影响[J].杂交水稻,22(2):64-68.[Huang F,Xie R,Liu C Y,Ye H Z,Yang W Y.2007.Effects of blast resistance of parents on that of their F1hybrids in rice[J].Hybrids Rice,22(2):64-68.]
冷祯陆.2011.78份水稻制恢材料的抗瘟性、农艺性状及杂种优势评价[D].成都:四川农业大学.[Leng Z L.2011.Evaluation of resistance to rice blast,main agronomic traits and heterosis of 78 rice materials of breeding restorer line[D].Chengdu:Sichuan Agricultural University.]
李建修.2012.水稻制恢材料对稻曲病、稻瘟病抗性及农艺性状评价[D].成都:四川农业大学.[Li J X.2012.Evaluation of resistance to rice false smut and rice blast,and agronomic characters evaluation of rice materials of breeding restorer line[D].Chengdu:Sichuan Agricultural University.]
李进斌,王甜,许明辉.2012.云南地方稻种抗稻瘟病基因Pita和Pi-b的鉴定[J].中国水稻科学,26(5):593-599.[Li J B,Wang T,Xu M H.2012.Identification of Pi-ta and Pi-b genes for rice blast resistance of rice landraces from Yunnan Province[J].Chinese Journal of Rice Science,26(5):593-599.]
刘洋,徐培洲,张红宇,徐建第,吴发强,吴先军.2008.水稻抗稻瘟病Pib基因的分子标记辅助选择与应用[J].中国农业科学,41(1):9-14.[Liu Y,Xu P Z,Zhang H Y,Xu JD,Wu F Q,Wu X J.2008.Marker-assisted selection and application of blast resistant gene Pib in rice[J].Scientia Agricultura Sinica,41(1):9-14.]
宋广树,孙蕾,刘志全,陈寞军,朱秀侠,周广春,封云,浦桂君,侯立刚.2016.吉林省主要水稻品种稻瘟病抗性分析及分子辅助改良[J].西北农业学报,25(5):677-683.[Song G S,Sun L,Liu Z Q,Chen M J,Zhu X X,Zhou G C,Feng Y,Pu G J,Hou L G.2016.Rice blast resistance analysis and molecular assistant improvement of main rice varieties in Jilin Province[J].Acta Agriculturae Boreali-occidentalis Sinica,25(5):677-683.]
孙国昌,杜新法,陶荣祥,孙漱沅.1998.水稻稻瘟病防治策略和21世纪研究展望[J].植物病理学报,28(4):289-292.[Sun G C,Du X F,Tao R X,Sun S Y.1998.Control tactics and prospect of rice blast research in 21thcentury[J].Acta Phytopathology Sinica,28(4):289-292.]
王丽丽,赵家铭,马作斌,郑文静,马殿荣.2017.辽宁地区水稻资源抗稻瘟病基因的检测分析[J].植物遗传资源学报,18(2):325-339.[Wang L L,Zhao J M,Ma Z B,Zheng W J,Ma D R.2017.Identificating and analyzing to rice blast gene in rice germplasm resources of Liaoning Province[J].Journal of Plant Genetic Resource,18(2):325-339.]
杨秀娟,朱春雨,阮宏椿,杜宜新,关瑞峰,陈福如.2008.福建省稻瘟病菌毒性类型及部分水稻品种(组合)抗病性[J].福建农林大学学报(自然科学版),37(3):244-247.[Yang X J,Zhu C Y,Ruan H C,Du Y X,Guan R F,Chen F R.2008.Pathogenic type of Magnaporthe grisea Barr.and the resistance of some rice cultivars to the pathogens in Fujian Province[J].Journal of Fujian Agriculture and Forestry University(National Science Edition),37(3):244-247.]
张雪梅.2011.四川省水稻主栽品种抗瘟性评价和内恢99-14抗瘟遗传性分析[D].成都:四川农业大学.[Zhang X M.2011.Resistance evaluation of rice cultivar from Sichuan Province and genetic analysis of Neihui 99-14 to blast disease[D].Chengdu:Sichuan Agricultural University.]
张银霞,田蕾,李培富.2016.宁夏自育水稻品种及部分引进品种的稻瘟病抗性基因分子标记检测[J].西北农业学报,25(7):989-996.[Zhang Y X,Tian L,Li P F.2016.Detection and analysis of rice blast resistant gene in rice varieties bred in Ningxia and introduced from outside Ningxia[J].Acta Agriculturae Boreali-occidentalis Sinica,25(7):989-996.]
张羽,冯志峰,崔明珠,张晓娟.2013.稻瘟病抗性基因Pi-km在陕西省稻种资源中的分布状况[J].分子植物育种,11(3):311-316.[Zhang Y,Feng Z F,Cui M Z,Zhang X J.2013.Distribution of blast resistance gene Pi-km in rice germplasms in Shaanxi Province of northwest China[J].Molecular Plant Breeding,11(3):311-316.]
Dean R,Van Kan J A L,Pretorius Z A,Hammond-Kosack KE,Pietro A D,Spanu P D,Rudd J J,Dickman M,Kahmann R,Ellis J,Foster G D.2012.The top 10 fungal pathogens in molecular plant pathology[J].Molecular Plant Pathology,13(4):414-430.
Deng Y W,Zhai K R,Xie Z,Yang D Y,Zhu X D,Liu J Z,Wang X,Qin P,Yang Y Z,Zhang G M,Li Q,Zhang J F,Wu S Q,Milazzo J,Mao B Z,Wang E T,Xie H A,Tharreau D,He Z H.2017.Epigenetic regulation of antagonistic receptors confers rice blast resistance with yield balance[J].Science,355(6238):962-965.
Jia Y L,McAdams S A,Bryan G T,Hershey H P,Valent B.2000.Direct interaction of resistance gene and avirulence gene products confers rice blast resistance[J].The EM-BO Journal,19(5):4004-4014.
Jia Y L,Wang Z H,Singh P.2002.Development of dominant rice blast Pita resistance gene markers[J].Crop Science,42(6):2145-2149
Moytri R,Jia Y L,Richard D C.2012.Structure,function,and co-evolution of rice blast resistance genes[J].Acta Agronomica Sinica,8(38):381-393.
Ramkumar G,Srinivasarao K,Madhan Mohan K,Sudarshan I,Sivaranjani A K P,Gopalakrishna K,Neeraja C N,Balachandran S M,Sundaram R M,Prasad M S,Shobha Rani N,Rama Prasad A M,Viraktamath B C,Madhav MS.2011.Development and validation of functional marker targeting an InDel in the major rice blast disease resistance gene Pi54(Pi-kh)[J].Molecular Breeding,27(1):129-135.
Skamnioti P,Gurr S J.2009.Against the grain:safeguarding rice from rice blast disease[J].Trends in Biotechnology,27(3):141-150.