基于UPLC-LTQ-Orbitrap-MS技术快速解析杜仲颗粒化学成分

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英文篇名：Rapid Identification of Chemical Compositions in Duzhong Granules by UPLC-LTQ-Orbitrap-MS Technique 作者：马永青 ; 王璐 ; 刘颖 ; 李欣欣 ; 刘永利 英文作者：MA Yong-qing;WANG Lu;LIU Ying;LI Xin-xin;LIU Yong-li;Hebei Institute for Drug Control;Hebei Province for Food and Drug Evaluation Certification Center;School of Traditional Chinese Pharmacy,China Pharmaceutical University; 关键词：杜仲颗粒 ; 超高效液相色谱-线性离子阱-静电场轨道阱组合式高分辨质谱(UPLC-LTQ-Orbitrap-MS) ; 定性分析 ; 黄酮类 ; 环烯醚萜类 英文关键词：Duzhong granules;;ultra high-performance liquid chromatography coupled with linear ion trap-orbitrap mass spectrometry(UPLC-LTQ-Orbitrap-MS);;qualitative analysis;;flavonoids;;iridoids 中文刊名：TEST 英文刊名：Journal of Instrumental Analysis 机构：河北省药品检验研究院;河北省食品药品审评认证中心;中国药科大学中药学院; 出版日期：2019-07-25 出版单位：分析测试学报 年：2019 期：v.38 基金：河北省科技项目(16272511) 语种：中文; 页：TEST201907010 页数：6 CN：07 ISSN：44-1318/TH 分类号：68-73

摘要

建立了定性分析杜仲颗粒化学成分的超高效液相色谱-线性离子阱-静电场轨道阱组合式高分辨质谱(UPLC-LTQ-Orbitrap-MS)方法。杜仲颗粒采用甲醇超声提取,Thermo Scientific Syncronis C_(18)色谱柱(100 mm×2.1 mm,1.9μm)分离,以乙腈-0.1%甲酸溶液为流动相进行梯度洗脱。利用LTQ-Orbitrap-MS负离子模式采集,通过对照品比对及高分辨质谱数据解析,鉴定了25种化学成分,包括8种环烯醚萜类、8种黄酮类、5种苯丙素类、3种木脂素类化合物及1种非还原性双糖。该方法简便、快速、准确,可为进一步分析杜仲颗粒的药效物质基础提供依据。
        An ultra high-performance liquid chromatography coupled with linear ion trap-orbitrap mass spectrometric(UPLC-LTQ-Orbitrap-MS) method was developed for the qualitative analysis of main chemical constituents in Duzhong granules,a Chinese medicine.The sample was ultrasonically extracted with methanol,then separated on a Thermo Scientific Syncronis C_(18) chromatographic column(100 mm×2.1 mm,1.9 μm) by gradient elution with acetonitrile-0.1% formic acid as mobile phase.The data were collected by using LTQ-Orbitrap-MS in negative ion mode.According to the contrast of the reference standards and the accurate masses of molecules,a total of 25 compounds in Duzhong granules were identified,including 8 iridoids,8 flavonoids,5 phenylpropanoids,3 lignans and 1 non-reducing disaccharide.Results showed that,with the advantages of simplicity,rapidness and accuracy,the developed method was suitable for the identification of chemical constituents in Duzhong granules,which provided a reference for the pharmacological basis of further analysis of Duzhong granules.

引文

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