有机磷中毒心肌损伤患者血清凋亡促进因子及抑制因子的表达变化研究
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摘要
目的:观察研究有机磷中毒心肌损伤患者血清凋亡促进因子及抑制因子的表达变化情况。方法:选取本院2017年6月-2018年6月收治的有机磷中毒心肌损伤患者50例作为A组,同期有机磷中毒无心肌损伤患者50例作为B组,另选取体检健康者50名作为C组。检测比较三组的血清凋亡促进因子(Caspase-3、Caspase-9)及抑制因子(Bcl-2、sFas、sFasL)的表达水平,以及A、B组不同中毒程度患者的上述检测指标表达水平。结果:A组的血清Caspase-3、Caspase-9、sFas及sFasL表达水平均明显高于B、C组,血清Bcl-2表达水平均明显低于B、C组,差异均有统计学意义(P<0.001);但B组与C组的上述检测指标表达水平比较,差异均无统计学意义(P>0.05)。A、B组不同中毒程度患者的上述检测指标表达水平比较,差异均有统计学意义(P<0.001)。结论:有机磷中毒心肌损伤患者血清凋亡促进因子及抑制因子的表达明显异常,在有机磷中毒心肌损伤患者中具有较高的检测价值。
Objective:To observe and study the expression change of serum apoptotic promoting factors and inhibiting factors of patients with myocardial injury caused by organophosphorus poisoning.Method:A total of 50 patients with myocardial injury caused by organophosphorus poisoning admitted in our hospital from June 2017 to June 2018 were selected as group A,50 patients with organophosphorus poisoning and without myocardial injury at the same time were selected as group B,and 50 healthy persons with physical examination were selected as group C.The expression levels of serum apoptotic promoting factor(Caspase-3 and Caspase-9)and inhibitory factor(Bcl-2,sFas and sFasL)of three groups were detected and compared,and the expression levels of the above-mentioned indicators in patients with different degrees of poisoning in group A and B were also detected and compared.Result:The expression levels of serum Caspase-3,Caspase-9,sFas and sFasL in group A were significantly higher than those in group B and C,the expression level of serum Bcl-2 was significantly lower than those in group B and C,the differences were statistically significant(P<0.001),but there were no significant differences in the expression levels of the above-mentioned indicators between group B and C(P>0.05).There were significant differences in the expression levels of the above-mentioned indicators in patients with different degrees of poisoning between group A and B(P<0.001).Conclusion:The expression of serum apoptotic promoting factors and inhibiting factors of patients with myocardial injury caused by organophosphorus poisoning are obviously abnormal and the detection value in patients with myocardial injury caused by organophosphorus poisoning are higher.
Objective:To observe and study the expression change of serum apoptotic promoting factors and inhibiting factors of patients with myocardial injury caused by organophosphorus poisoning.Method:A total of 50 patients with myocardial injury caused by organophosphorus poisoning admitted in our hospital from June 2017 to June 2018 were selected as group A,50 patients with organophosphorus poisoning and without myocardial injury at the same time were selected as group B,and 50 healthy persons with physical examination were selected as group C.The expression levels of serum apoptotic promoting factor(Caspase-3 and Caspase-9)and inhibitory factor(Bcl-2,sFas and sFasL)of three groups were detected and compared,and the expression levels of the above-mentioned indicators in patients with different degrees of poisoning in group A and B were also detected and compared.Result:The expression levels of serum Caspase-3,Caspase-9,sFas and sFasL in group A were significantly higher than those in group B and C,the expression level of serum Bcl-2 was significantly lower than those in group B and C,the differences were statistically significant(P<0.001),but there were no significant differences in the expression levels of the above-mentioned indicators between group B and C(P>0.05).There were significant differences in the expression levels of the above-mentioned indicators in patients with different degrees of poisoning between group A and B(P<0.001).Conclusion:The expression of serum apoptotic promoting factors and inhibiting factors of patients with myocardial injury caused by organophosphorus poisoning are obviously abnormal and the detection value in patients with myocardial injury caused by organophosphorus poisoning are higher.
引文
[1]王鑫,李爱军,李伟哲,等.血必净对急性百草枯中毒大鼠肺损伤细胞凋亡及炎性因子的影响[J].中华劳动卫生职业病杂志,2018,36(7):551-555.
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[10]张清,李春盛.两种血液净化方式对重度急性有机磷中毒患者心肌损伤疗效的比较[J].中华急诊医学杂志,2016,25(4):495-498.
[11]韩学毅,李敏雄,区彩琼,等.血液灌流联合连续性静脉-静脉血液滤过治疗对急性有机磷农药中毒患者的心肌保护作用[J].南昌大学学报(医学版),2017,57(1):46-49.
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[15]林强康,杨絮,林岳,等.不同血液净化方案下重度急性有机磷农药中毒患者的心肌状况对比[J].中国生化药物杂志,2017,37(5):419-421.
[16]陈士轩,滕哈乐,牛红霞,等.急性有机磷农药中毒心肌损伤最佳标志物的研究[J].中国医药导报,2015,12(31):97-100.
[17]李敬,张建军,李娜,等.和肽素及缺血修饰白蛋白在急性有机磷农药中毒早期心肌损伤中的应用[J].南方医科大学学报,2015,35(3):459-462.
[18]张珂,冯慧,周凤芹,等.左西孟旦改善急性有机磷农药中毒患者心功能效果观察[J].山东医药,2017,57(46):64-66.
[19]李振翮,王贵霞,甄国栋,等.影响急性有机磷中毒合并中间综合征预后的相关因素分析[J].中华急诊医学杂志,2016,25(11):1406-1409.
[20]杜斌,陈炜,陈俊良,等.α-硫辛酸对百草枯中毒大鼠胸腺Bax、Bcl-2表达及SOD活力、MDA水平的影响[J].中国医院药学杂志,2015,35(23):2071-2074.
[2]李海峰,邢宝鹏,权玉兰,等.乌司他丁对百草枯中毒大鼠肺细胞自噬和凋亡的影响[J].中华急诊医学杂志,2015,24(2):169-174.
[3]商跃云,张慧,林书祥,等.急性百草枯中毒患儿外周血CD4+T淋巴细胞肌醇依赖酶1、凋亡信号调节激酶1及c-Jun氨基末端激酶水平变化的意义[J].中华实用儿科临床杂志,2017,32(6):447-450.
[4]王鑫,赵艳霞,李海霞,等.Caspase-8、Caspase-9在急性百草枯中毒大鼠肾组织中的表达及作用研究[J].现代中西医结合杂志,2015,24(16):1734-1737.
[5]李永胜,杨蓉佳,张世魁,等.前列地尔注射液对急性重度有机磷农药中毒患者的心肌保护效果[J].临床荟萃,2015,30(9):981-984.
[6]Peter J V,Sudarsan T I,Moran J L.Clinical features of organophosphate poisoning:a review of different classification systems and approaches[J].Indian J Crit Care Med,2014,18(11):735-745.
[7]Pamies D,Sogorb M A,Fabbri M,et al.Genomic and phenotypic alterations of the neuronal-like cells derived from human embryonal carcinoma stem cells(NT2)caused by exposure to organophosphorus compounds paraoxon and mipafox[J].Int J Mol Sci,2014,15(1):905-926.
[8]赵艳霞,付赟,王鑫.血必净对急性百草枯中毒大鼠肾脏细胞凋亡及其调控因子Bcl-2、Bax、Caspase-3表达的影响[J].河北医药,2018,40(19):2889-2894.
[9]王晔,曲艳,龙波.核结合因子-κB及bcl-2/bax在PQ中毒鼠肺组织中的表达[J].昆明医科大学学报,2015,36(3):30-33.
[10]张清,李春盛.两种血液净化方式对重度急性有机磷中毒患者心肌损伤疗效的比较[J].中华急诊医学杂志,2016,25(4):495-498.
[11]韩学毅,李敏雄,区彩琼,等.血液灌流联合连续性静脉-静脉血液滤过治疗对急性有机磷农药中毒患者的心肌保护作用[J].南昌大学学报(医学版),2017,57(1):46-49.
[12]Eddleston M,Chowdhury F R.Pharmacological treatment of organophosphorus insecticide poisoning:the old and the(possible)new[J].Br J Clin Pharmacol,2016,81(3):462-470.
[13]Kumar S,Diwan S K,Dubey S.Myocardial infarction in organophosphorus poisoning:association or just chance?[J].J Emerg Trauma Shock,2014,7(2):131-132.
[14]王英娟,张春容.血必净治疗急性有机磷农药中毒致心肌损害的疗效及机制探讨[J].中国社区医师,2017,33(16):90-91.
[15]林强康,杨絮,林岳,等.不同血液净化方案下重度急性有机磷农药中毒患者的心肌状况对比[J].中国生化药物杂志,2017,37(5):419-421.
[16]陈士轩,滕哈乐,牛红霞,等.急性有机磷农药中毒心肌损伤最佳标志物的研究[J].中国医药导报,2015,12(31):97-100.
[17]李敬,张建军,李娜,等.和肽素及缺血修饰白蛋白在急性有机磷农药中毒早期心肌损伤中的应用[J].南方医科大学学报,2015,35(3):459-462.
[18]张珂,冯慧,周凤芹,等.左西孟旦改善急性有机磷农药中毒患者心功能效果观察[J].山东医药,2017,57(46):64-66.
[19]李振翮,王贵霞,甄国栋,等.影响急性有机磷中毒合并中间综合征预后的相关因素分析[J].中华急诊医学杂志,2016,25(11):1406-1409.
[20]杜斌,陈炜,陈俊良,等.α-硫辛酸对百草枯中毒大鼠胸腺Bax、Bcl-2表达及SOD活力、MDA水平的影响[J].中国医院药学杂志,2015,35(23):2071-2074.