菌糠炭与微生物协同吸附-降解石油烃类污染物
|
摘要
以菌糠为原材料,在不同热解温度(250～650℃)下限氧热解制备菌糠炭,通过分析菌糠及菌糠炭结构的差异,探究其对微生物、石油烃的吸附性能及固定化菌株苍白杆菌Q1对石油烃的降解效果。结果表明:随着热解温度升高,菌糠炭对微生物吸附效果提高,其中550℃菌糠炭吸附固定化量最高为1.582×10~(10) CFU/g,SEM扫面电镜结果显示菌株主要吸附在材料表面。高温炭对石油烃吸附较好,其中550℃菌糠炭对胶质、沥青质吸附率最高,分别为36.33%、25.59%;吸附效果均与孔结构、芳香性相关显著,其协同微生物对石油烃四组分总体降解效率高,均优于其他热解温度下制备的菌糠炭组,pH值和有机碳含量对微生物吸附-降解影响较明显,550℃菌糠炭对微生物降解石油烃具有强化作用。
Biochar samples were prepared at different pyrolysis temperatures(250-650 ℃) using spent mushroom substrate(SMS) as raw materials. Effect of different biochar structures on adsorption rate of Ochrobactrum sp. Q1, oil and degradation rate were analyzed. Experimental results indicated that 550 ℃-biochar(BC550) has the best adsorption performance(i.e., 1.582×10~(10) CFU/g) on microorganism. SEM analyses showed that most microorganisms were immobilized on the surface of the materials. BC550 also has the best adsorption performance on resin and asphaltenes, which were 36.33% and 25.59%, respectively. It was found that adsorption performance is strongly related with pore structure and aromaticity. BC550 immobilized bacteria have better degradation rate for different oil components(i.e., saturates, aromatics, resins and asphaltenes) than other biochar groups prepared at other different pyrolysis temperatures. In addition, pH value and organic carbon content have obvious effect on microbial adsorption-degradation rate. BC550 has enhanced effect on microbial degradation of petroleum hydrocarbons.
Biochar samples were prepared at different pyrolysis temperatures(250-650 ℃) using spent mushroom substrate(SMS) as raw materials. Effect of different biochar structures on adsorption rate of Ochrobactrum sp. Q1, oil and degradation rate were analyzed. Experimental results indicated that 550 ℃-biochar(BC550) has the best adsorption performance(i.e., 1.582×10~(10) CFU/g) on microorganism. SEM analyses showed that most microorganisms were immobilized on the surface of the materials. BC550 also has the best adsorption performance on resin and asphaltenes, which were 36.33% and 25.59%, respectively. It was found that adsorption performance is strongly related with pore structure and aromaticity. BC550 immobilized bacteria have better degradation rate for different oil components(i.e., saturates, aromatics, resins and asphaltenes) than other biochar groups prepared at other different pyrolysis temperatures. In addition, pH value and organic carbon content have obvious effect on microbial adsorption-degradation rate. BC550 has enhanced effect on microbial degradation of petroleum hydrocarbons.
引文
[1]李佳,曹兴涛,隋红,等.石油污染土壤修复技术研究现状与展望[J].石油学报(石油加工),2017,33(5):811-833.(LI Jia,CAO Xingtao,SUI Hong,et al.Overview of remediation technologies for petroleumcotaminated soil[J].Acta Petrolei Sinica(Petroleum Processing Section),2017,33(5):811-833.)
[2]李杨,李凡修.石油污染土壤的微生物修复技术[J].化工环保,2017,37(6):605-610.(LI Yang,LIFanxiu.Microbial remediation technology for petroleum contaminated soil[J].Environmental Protection of Chemical Industry,2017,37(6):605-610.)
[3]王玉建,李红玉.固定化微生物在废水处理中的研究及进展[J].生物技术,2006,16(1):94-96.(WANGYujian,LI Hongyu.Advances in immobilized microorganism and it’s research on waste water treatment[J].Biotechnology,2006,16(1):94-96.)
[4]李亚娇,孙国琴,郭九峰,等.食用菌菌糠利用的最新研究进展[J].中国食用菌,2017,36(4):1-4.(LIYajiao,SUN Guoqin,GUO Jiufeng,et al.Latest research progress and utilization on spent mushroom substrate[J].Edible Fungi of China,2017,36(4):1-4.)
[5]LI X,WU Y,LIN X,et al.Dissipation of polycyclic aromatic hydrocarbons(PAHs)in soil microcosms amended with mushroom cultivation substrate[J].Soil Biology and Biochemistry,2012,47:191-197.
[6]GARCA-DELGADO C,YUNTA F,EYMAR E.Bioremediation of multi-polluted soil by spent mushroom(Agaricus bisporus)substrate:Polycyclic aromatic hydrocarbons degradation and Pb availability[J].Journal of Hazardous Materials,2015,300:281-288.
[7]ASEMOLOYE M D,JONATHAN S G,JAYEOLA A A,et al.Mediational influence of spent mushroom compost on phytoremediation of black-oil hydrocarbon polluted soil and response of Megathyrsus maximus Jacq[J].Journal of Environmental Management,2017,200:253-262.
[8]李晓娜,宋洋,贾明云,等.生物质炭对有机污染物的吸附及机理研究进展[J].土壤学报,2017,54(6):1313-1325.(LI Xiaona,SONG Yang,JIA Mingyun,et al.A review of researches on biochar adsorbing organic contaminants and its mechanism[J].Acta Pedologica Sinica,2017,54(6):1313-1325.)
[9]马锋锋,赵保卫.不同热解温度制备的玉米芯生物炭对对硝基苯酚的吸附作用[J].环境科学,2017,38(2):837-844.(MA Fengfeng,ZHAO Baowei.Sorption of p-nitrophenol by biochars of corncob prepared at different pyrolysis temperatures[J].Environmental Science,2017,38(2):837-844.)
[10]高凯芳,简敏菲,余厚平,等.裂解温度对稻秆与稻壳制备生物炭表面官能团的影响[J].环境化学,2016,35(8):1663-1669.(GAO Kaifang,JIAN Minfei,YUHouping,et al.Effects of pyrolysis temperatures on the biochars and its surface functional groups made from rice straw and rice husk[J].Environmental Chemistry,2016,35(8):1663-1669.)
[11]张秀霞,张守娟,张涵,等.固定化微生物对石油污染土壤生物学特性的影响[J].石油学报(石油加工),2015,31(1):112-118.(ZHANG Xiuxia,ZHANGShoujuan,ZHANG Han,et al.Influence of immobilized microorganism on biological characteristicis of petroleum-contaminated soil[J].Acta Petrolei Sinica(Petroleum Processing Section),2015,31(1):112-118.)
[12]迟建国,吕吉利,高永超,等.生物炭固定化菌剂对含油海水中石油的去除[J].工业安全与环保,2015,41(6):13-16.(CHI Jianguo,LJili,GAO Yongchao,et al.Crude oil removal from contaminated sea water with biochar immobilized microbial agents[J].Industrial Safety and Environmental Protection,2015,41(6):13-16.)
[13]张守娟.固定化微生物对石油污染土壤环境因子的调控作用研究[D].青岛:中国石油大学(华东),2014.
[14]BOEHM H P.Some aspects of the surface chemistry of carbon blacks and other carbons[J].Carbon,1994,32(5):759-769.
[15]郑孟杰,李继洲,靳红梅,等.沉水植物生物炭对Cr~(6+)和磷的吸附特性[J].生态与农村环境学报,2017,33(12):1132-1139.(ZHENG Mengjie,LIJizhou,JIN Hongmei,et al.Characterization of Cr6+and phosphorus adsorptions of biochars derived from submerged plants[J].Journal of Ecology and Rural Environment,2017,33(12):1132-1139.)
[16]杜勇.生物炭固定化微生物去除水中苯酚的研究[D].重庆:重庆大学,2012.
[17]张昭.两株来自深海热液区的嗜热铁还原细菌的分离鉴定及其铁还原机制的研究[D].厦门:厦门大学,2013.
[18]孔露露,周启星.新制备生物炭的特性表征及其对石油烃污染土壤的吸附效果[J].环境工程学报,2015,9(5):2462-2468.(KONG Lulu,ZHOU Qixing.Characterization of new-prepared biochars and their adsorption effectiveness on petroleum hydrocarbon contaminated soil[J].Chinese Journal of Environmental Engineering,2015,9(5):2462-2468.)
[19]侯建华.多孔碳的结构设计及其电化学储能性能研究[D].北京:北京理工大学,2015.
[20]张秀霞,徐娜娜,秦丽姣,等.固定化微生物降解石油的影响因素研究[J].安全与环境学报,2011,11(5):77-81.(ZHANG Xiuxia,XU Nana,QIN Lijiao,et al.Study on the influential factors of immobilized microorganisms biodegrading oil[J].Journal of Safety and Environment,2011,11(5):77-81.)
[21]LPEZ-MIRANDA J,CISNEROS-DE LA CUEVA S,PEZ-LERMA J B,et al.Changes in hydrocarbon composition and autochthonous microorganism growth of contaminated mining soil during bioremediation[J].Water,Air,&Soil Pollution,2018,229:165.
[22]李政,梁昌峰,赵朝成,等.应用SPSS软件分析石油污染土壤微生态环境[J].石油学报(石油加工),2012,28(2):345-351.(LI Zheng,LIANG Changfeng,ZHAO Chaocheng,et al.Analysis of microbial ecosystem in petroleum polluted soils by statistic analysis software SPSS[J].Acta Petrolei Sinica(Petroleum Processing Section),2012,28(2):345-351.)
[2]李杨,李凡修.石油污染土壤的微生物修复技术[J].化工环保,2017,37(6):605-610.(LI Yang,LIFanxiu.Microbial remediation technology for petroleum contaminated soil[J].Environmental Protection of Chemical Industry,2017,37(6):605-610.)
[3]王玉建,李红玉.固定化微生物在废水处理中的研究及进展[J].生物技术,2006,16(1):94-96.(WANGYujian,LI Hongyu.Advances in immobilized microorganism and it’s research on waste water treatment[J].Biotechnology,2006,16(1):94-96.)
[4]李亚娇,孙国琴,郭九峰,等.食用菌菌糠利用的最新研究进展[J].中国食用菌,2017,36(4):1-4.(LIYajiao,SUN Guoqin,GUO Jiufeng,et al.Latest research progress and utilization on spent mushroom substrate[J].Edible Fungi of China,2017,36(4):1-4.)
[5]LI X,WU Y,LIN X,et al.Dissipation of polycyclic aromatic hydrocarbons(PAHs)in soil microcosms amended with mushroom cultivation substrate[J].Soil Biology and Biochemistry,2012,47:191-197.
[6]GARCA-DELGADO C,YUNTA F,EYMAR E.Bioremediation of multi-polluted soil by spent mushroom(Agaricus bisporus)substrate:Polycyclic aromatic hydrocarbons degradation and Pb availability[J].Journal of Hazardous Materials,2015,300:281-288.
[7]ASEMOLOYE M D,JONATHAN S G,JAYEOLA A A,et al.Mediational influence of spent mushroom compost on phytoremediation of black-oil hydrocarbon polluted soil and response of Megathyrsus maximus Jacq[J].Journal of Environmental Management,2017,200:253-262.
[8]李晓娜,宋洋,贾明云,等.生物质炭对有机污染物的吸附及机理研究进展[J].土壤学报,2017,54(6):1313-1325.(LI Xiaona,SONG Yang,JIA Mingyun,et al.A review of researches on biochar adsorbing organic contaminants and its mechanism[J].Acta Pedologica Sinica,2017,54(6):1313-1325.)
[9]马锋锋,赵保卫.不同热解温度制备的玉米芯生物炭对对硝基苯酚的吸附作用[J].环境科学,2017,38(2):837-844.(MA Fengfeng,ZHAO Baowei.Sorption of p-nitrophenol by biochars of corncob prepared at different pyrolysis temperatures[J].Environmental Science,2017,38(2):837-844.)
[10]高凯芳,简敏菲,余厚平,等.裂解温度对稻秆与稻壳制备生物炭表面官能团的影响[J].环境化学,2016,35(8):1663-1669.(GAO Kaifang,JIAN Minfei,YUHouping,et al.Effects of pyrolysis temperatures on the biochars and its surface functional groups made from rice straw and rice husk[J].Environmental Chemistry,2016,35(8):1663-1669.)
[11]张秀霞,张守娟,张涵,等.固定化微生物对石油污染土壤生物学特性的影响[J].石油学报(石油加工),2015,31(1):112-118.(ZHANG Xiuxia,ZHANGShoujuan,ZHANG Han,et al.Influence of immobilized microorganism on biological characteristicis of petroleum-contaminated soil[J].Acta Petrolei Sinica(Petroleum Processing Section),2015,31(1):112-118.)
[12]迟建国,吕吉利,高永超,等.生物炭固定化菌剂对含油海水中石油的去除[J].工业安全与环保,2015,41(6):13-16.(CHI Jianguo,LJili,GAO Yongchao,et al.Crude oil removal from contaminated sea water with biochar immobilized microbial agents[J].Industrial Safety and Environmental Protection,2015,41(6):13-16.)
[13]张守娟.固定化微生物对石油污染土壤环境因子的调控作用研究[D].青岛:中国石油大学(华东),2014.
[14]BOEHM H P.Some aspects of the surface chemistry of carbon blacks and other carbons[J].Carbon,1994,32(5):759-769.
[15]郑孟杰,李继洲,靳红梅,等.沉水植物生物炭对Cr~(6+)和磷的吸附特性[J].生态与农村环境学报,2017,33(12):1132-1139.(ZHENG Mengjie,LIJizhou,JIN Hongmei,et al.Characterization of Cr6+and phosphorus adsorptions of biochars derived from submerged plants[J].Journal of Ecology and Rural Environment,2017,33(12):1132-1139.)
[16]杜勇.生物炭固定化微生物去除水中苯酚的研究[D].重庆:重庆大学,2012.
[17]张昭.两株来自深海热液区的嗜热铁还原细菌的分离鉴定及其铁还原机制的研究[D].厦门:厦门大学,2013.
[18]孔露露,周启星.新制备生物炭的特性表征及其对石油烃污染土壤的吸附效果[J].环境工程学报,2015,9(5):2462-2468.(KONG Lulu,ZHOU Qixing.Characterization of new-prepared biochars and their adsorption effectiveness on petroleum hydrocarbon contaminated soil[J].Chinese Journal of Environmental Engineering,2015,9(5):2462-2468.)
[19]侯建华.多孔碳的结构设计及其电化学储能性能研究[D].北京:北京理工大学,2015.
[20]张秀霞,徐娜娜,秦丽姣,等.固定化微生物降解石油的影响因素研究[J].安全与环境学报,2011,11(5):77-81.(ZHANG Xiuxia,XU Nana,QIN Lijiao,et al.Study on the influential factors of immobilized microorganisms biodegrading oil[J].Journal of Safety and Environment,2011,11(5):77-81.)
[21]LPEZ-MIRANDA J,CISNEROS-DE LA CUEVA S,PEZ-LERMA J B,et al.Changes in hydrocarbon composition and autochthonous microorganism growth of contaminated mining soil during bioremediation[J].Water,Air,&Soil Pollution,2018,229:165.
[22]李政,梁昌峰,赵朝成,等.应用SPSS软件分析石油污染土壤微生态环境[J].石油学报(石油加工),2012,28(2):345-351.(LI Zheng,LIANG Changfeng,ZHAO Chaocheng,et al.Analysis of microbial ecosystem in petroleum polluted soils by statistic analysis software SPSS[J].Acta Petrolei Sinica(Petroleum Processing Section),2012,28(2):345-351.)