摘要
采用磷酸盐缓冲溶液法从脱脂椰子粉中提取椰子蛋白,在单因素试验的基础上,通过Plackett-Burman试验和响应面分析法对椰子蛋白提取工艺进行优化,结果表明:最优工艺条件为碱溶时间92 mim、碱溶pH 10.0、酸沉时间116 min、酸沉pH 4.5,在该条件下,椰子分离蛋白提取率为83.18%。
The coconut protein was extracted from defatted coconut powder by phosphate buffer solution. Based on the single factor test, the coconut protein extraction process was optimized by Plackett-Burman test and response surface analysis. The results showed that the optimal process conditions were as follows: alkali dissolution time 92 min, alkali solution pH 10.0, acid precipitation time 116 min, acid precipitation pH 4.5, and the extraction rate of coconut protein isolate was 83.18% under these conditions.
引文
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