微波辅助提取薏苡仁油工艺优化
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摘要
采用微波辅助提取法研究了薏苡仁中薏苡仁油的提取工艺。在单因素实验基础上,采用Box-Behnken试验设计结合响应面分析法,对薏苡仁油的提取工艺进行优化。单因素实验结果表明,薏苡仁油最佳提取液料比为12∶1 mL/g、微波提取温度为60℃、微波提取时间为15 min、微波提取功率600 W。响应面试验结果表明,薏苡仁油提取的最优工艺参数为液料比为12.39∶1 mL/g,微波提取温度为60℃,微波提取时间为920 s,微波提取功率为621 W。在此条件下,薏苡仁油得率可达9.31%±0.10%,与预测值9.41%接近,说明响应面法优化的薏苡仁油微波辅助提取工艺具有可行性。
The extraction technology of coix seed oil was studied by microwave-assisted extraction.Based on single factor experiment,the Box-Behnken experimental design combined with response surface optimization was applied to optimize the extraction process of coix seed oil.The single factor experiment results showed that the ratio of ethanol to material was 12∶1 mL/g,microwave extraction temperature was 60 ℃,microwave extraction duration was 15 min,microwave extraction power was 600 W,respectively. The results of response surface test showed that the optimal process parameters were ratio of ethanol to material 12.39∶1 mL/g,microwave extraction temperature 60 ℃,microwave extraction duration 920 s,microwave extraction power 621 W. Under these condition,the yield of coix seed oil reached to 9.31%±0.10%,which was close to the predicted value of 9.41%,indicating that the microwave-assisted extraction process of coix seed oil optimized by response surface method was feasible.
The extraction technology of coix seed oil was studied by microwave-assisted extraction.Based on single factor experiment,the Box-Behnken experimental design combined with response surface optimization was applied to optimize the extraction process of coix seed oil.The single factor experiment results showed that the ratio of ethanol to material was 12∶1 mL/g,microwave extraction temperature was 60 ℃,microwave extraction duration was 15 min,microwave extraction power was 600 W,respectively. The results of response surface test showed that the optimal process parameters were ratio of ethanol to material 12.39∶1 mL/g,microwave extraction temperature 60 ℃,microwave extraction duration 920 s,microwave extraction power 621 W. Under these condition,the yield of coix seed oil reached to 9.31%±0.10%,which was close to the predicted value of 9.41%,indicating that the microwave-assisted extraction process of coix seed oil optimized by response surface method was feasible.
引文
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[19]胡滨,潘婉舒,刘换丽,等.微波辅助提取榛子油及其脂肪酸组成分析[J].中国食品学报,2016,16(12):151-161.
[20]胡滨,陈一资,杨勇,等.响应面法优化微波辅助提取松籽油的工艺研究[J].中国粮油学报,2016,31(1):52-59.
[21]徐华,张茹,刘连亮,等.辣木籽油脂微波提取工艺优化及其脂质分析[J].核农学报,2018,32(7):1393-1399.
[22]杜邵龙,周春山.微波辅助提取薏苡仁油的研究[J].中国粮油学报,2006,21(2):79-81.
[23]金宏,朱庆书,赵文英,等.微波法提取薏苡仁油的研究[J].化学与生物工程,2009,26(7):52-54.
[24]贺东亮,张恒慧,李静舒,等.响应面法优化绿薄荷精油的超临界CO2萃取工艺[J].食品研究与开发,2018,39(10):61-66.
[2]胡少华,肖小年,易醒,等.薏苡仁的研究新进展[J].时珍国医国药,2009,20(5):1059-1060.
[3]梁金梅.薏苡仁汤治疗类风湿关节炎、发炎性皮肤病的临床研究[D].武汉:湖北中医药大学,2012:9-10.
[4]Hu Y,Liang H,Gong W K,et al.Effect of jobstears seed oil on telomerase activity of rat mesangial cells in vitro[J].Chinese Journal of Pharmacology and Toxicology,2005(6):452-454.
[5]张洋婷,马洪波,宋春梅,等.薏苡仁多糖的提取及其对油脂的抗氧化作用研究[J].食品研究与开发,2015,36(18):58-61.
[6]吴岩,原永芳.薏苡仁的化学成分和药理活性研究进展[J].华西药学杂志,2010,25(1):111-113.
[7]陶小军,闫宇辉,徐志立,等.薏苡仁油抗炎消肿作用研究[J].辽宁中医药大学报,2015,17(1):45-46.
[8]Liang X Y,Ding Z H.The pharmacological action and clinical application of coix seed oil on digestive system tumors[J].Journal of Practical Medicine,2017,33(1):159-161.
[9]Zhan Y P,Huang X E,Cao J,et al.Clinical safety and efficacy of Kanglaite(coix seed oil)injection combined with chemotherapy in treating patients with gastric cancer[J].Asian Pacific Journal Cancer Prevention Apjcp,2012,13(10):5319-5321.
[10]Xi X J,Zhu Y G,Tong Y P,et al.Assessment of the genetic diversity of different job’s tears(Coix lacryma-jobi L.)accessions and the active composition and anticancer effect of its seed oil[J].Plos One,2016,11(4):e0153269.
[11]郑利,陈丹,范世明,等.薏苡仁油的质量分析[J].福建医科大学学报,2016,50(4):222-226.
[12]徐俊丽,张明丽.细胞免疫治疗联合注射用薏苡仁油对老年肝癌患者T细胞亚群及疾病预后的影响[J].中国临床研究,2016,29(8):1054-1056.
[13]许健,沈雯,孙金权,等.薏苡仁油对人原位胰腺癌Bx PC-3细胞生长及VEGF和bFGF表达的影响[J].中草药,2012,43(4):724-728.
[14]张明发,沈雅琴.薏苡仁油抗白血病和肾癌药理作用的研究近况[J].抗感染药学,2012,9(4):252-256.
[15]谭兵,吴府容,白玉,等.注射用薏苡仁油在晚期非小细胞肺癌化疗中的作用[J].肿瘤学杂志,2014,20(6):460-463.
[16]Yu Y L,Lu Y,Tang X,et al.Formulation,preparation and evaluation of an intravenous emulsion containing Brucea javanica oil and coix seed oil for anti-tumor application[J].Biological and Pharmaceubial Bulletin,2008,31(4):673-680.
[17]Woo J H,Dapeng L,Orita H,et al.Coix seed extract,a commonly used treatment for cancer in china,inhibits NFκB and protein kinase C signaling[J].Cancer Biology and Therapy,2007,6(12):2005-2011.
[18]李毓,胡笑克,吴棣华,等.薏苡仁酯对人鼻咽癌细胞裸鼠移植瘤的治疗作用[J].肿瘤防治研究,2001,28(5):356-358.
[19]胡滨,潘婉舒,刘换丽,等.微波辅助提取榛子油及其脂肪酸组成分析[J].中国食品学报,2016,16(12):151-161.
[20]胡滨,陈一资,杨勇,等.响应面法优化微波辅助提取松籽油的工艺研究[J].中国粮油学报,2016,31(1):52-59.
[21]徐华,张茹,刘连亮,等.辣木籽油脂微波提取工艺优化及其脂质分析[J].核农学报,2018,32(7):1393-1399.
[22]杜邵龙,周春山.微波辅助提取薏苡仁油的研究[J].中国粮油学报,2006,21(2):79-81.
[23]金宏,朱庆书,赵文英,等.微波法提取薏苡仁油的研究[J].化学与生物工程,2009,26(7):52-54.
[24]贺东亮,张恒慧,李静舒,等.响应面法优化绿薄荷精油的超临界CO2萃取工艺[J].食品研究与开发,2018,39(10):61-66.