金线莲多糖对Hep1-6细胞活性的影响
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摘要
通过采用MTS法、流式细胞术法以及构建RAW264.7/Hep1-6细胞共培养模型检测经不同浓度(0、125、250、500、1 000、2 000μg/mL)金线莲多糖(Anoectochilus roxburghii Polysaccharides, APS)处理的RAW264.7细胞和Hep1-6细胞的增殖及周期情况,研究APS对Hep1-6细胞生长的影响.直接使用APS处理Hep1-6细胞,发现不同浓度APS对Hep1-6细胞增殖无抑制作用,且对其周期无影响.由共培养模型可知,不同浓度APS先作用于RAW264.7细胞24 h后,将RAW264.7细胞及其培养液与Hep1-6细胞共培养24 h后,0、125、500μg/mL试验组与不加药无RAW264.7空白对照组相比,Hep1-6细胞受到一定抑制作用,细胞毒性为1级. 125、500μg/mL试验组与不加药含RAW264.7对照组相比,Hep1-6细胞具有显著抑制(P <0.01),但2 000μg/mL试验组中Hep1-6细胞生长率高于对照组. APS直接作用于Hep1-6对其活性无影响,而RAW264.7与Hepl-6共培养后,RAW264.7可以抑制Hepl-6的生长,加APS处理RAW264.7后再与Hepl-6共培养,一定浓度范围内可以进一步抑制Hepl-6的生长,但过高浓度的APS反而对共培养的Hepl-6生长有一定促进作用.
The MTS assay was used to detect the cell proliferation and the flow cytometry was used to detect the cell cycle that could reflect the growth of Hep1-6 cell and RAW264.7 cell which were treated by APS of different concentrations. The effect concentration was 0, 125, 250, 500, 1 000 and 2 000 μg/mL. The cell co-culture model was constructed to detect the effect of RAW264.7 on the growth of Hep1-6. The date suggested that APS didnt inhibit the cell proliferation and had no effect on the cell cycle of Hep1-6 cell and RAW264.7 cell. It was found that the growth of Hep1-6 cell was inhibited when RAW264.7 cell treated by different concentrations of APS which were 0, 125 and 500 μg/mL for 24 hours co-cultured with Hep1-6 cell compared with the blank control group. The cytotoxicity was level 1. Hep1-6 cell were significantly inhibited in the 125, 500 μg/mL test group compared with the non-treated RAW264.7 cell control group(P < 0.01), but the growth rate of Hep1-6 cell in the2 000 μg/mL test group was higher than that in the control group. APS has no effect on Hep1-6 cell activity when APS directly treats cell. RAW264.7 cell can inhibit the growth of Hepl-6 cell while RAW264.7 cell and Hepl-6 cell co-culture. The growth of Hepl-6 cell can be further inhibited within a certain concentration range when RAW264.7 cell treated by APS firstly and then co-culture with Hepl-6 cell, but the excessive concentration of APS can promote the growth of Hepl-6 cell.
The MTS assay was used to detect the cell proliferation and the flow cytometry was used to detect the cell cycle that could reflect the growth of Hep1-6 cell and RAW264.7 cell which were treated by APS of different concentrations. The effect concentration was 0, 125, 250, 500, 1 000 and 2 000 μg/mL. The cell co-culture model was constructed to detect the effect of RAW264.7 on the growth of Hep1-6. The date suggested that APS didnt inhibit the cell proliferation and had no effect on the cell cycle of Hep1-6 cell and RAW264.7 cell. It was found that the growth of Hep1-6 cell was inhibited when RAW264.7 cell treated by different concentrations of APS which were 0, 125 and 500 μg/mL for 24 hours co-cultured with Hep1-6 cell compared with the blank control group. The cytotoxicity was level 1. Hep1-6 cell were significantly inhibited in the 125, 500 μg/mL test group compared with the non-treated RAW264.7 cell control group(P < 0.01), but the growth rate of Hep1-6 cell in the2 000 μg/mL test group was higher than that in the control group. APS has no effect on Hep1-6 cell activity when APS directly treats cell. RAW264.7 cell can inhibit the growth of Hepl-6 cell while RAW264.7 cell and Hepl-6 cell co-culture. The growth of Hepl-6 cell can be further inhibited within a certain concentration range when RAW264.7 cell treated by APS firstly and then co-culture with Hepl-6 cell, but the excessive concentration of APS can promote the growth of Hepl-6 cell.
引文
[1] TORRE L A, BRAY F, SIEGEL R L, et al. Global cancer statistic, 2012[J]. CA Cancer J Clin, 2015, 65(2):87-108.
[2] CHEN W, ZHENG R, BAADE P D, et al. Cancer statistics in China, 2015[J]. CA Cancer J Clin, 2016, 66(2):115-132.
[3]洪琳,邵清松,周爱存,等.金线莲产业现状及可持续发展对策[J].中国中药杂志,2016,41(3):553-558.
[4]郭慧慧,蒋元斌,林丛发,等.金线莲研究进展[J].福建农业科技,2016(03):55-59.
[5]吴丽丽,梁燕,许光辉.金线莲化学成分、药理作用及临床应用研究概述[J].海峡药学,2014(10):34-37.
[6] GETACHEW Y, CUSIMANO F A, JAMES L P, et al. The role of intrahepatic CD3+/CD4-/CD8-double negative T(DN T)cells in enhanced acetaminophen toxicity[J]. Toxicology&Applied Pharmacology, 2014, 280(2):264-271.
[7]张多强,辛国军,丁洋,等.枸杞多糖对HepG2肝癌细胞自噬与细胞凋亡的影响[J].中成药,2017(12):2600-2602.
[8]刘栩岑,李玉洁,王娅杰,等.参莲提取物对PM(2.5)染毒RAW264.7细胞损伤的保护作用[J].中国中药杂志,2015,40(10):1977-1983.
[9]代艳文,袁丁,万静枝,等.竹节参总皂苷通过NF-κB通路对LPS致RAW264.7细胞炎症的保护作用研究[J].中国中药杂志,2014,39(11):2076.
[10]李康,郭强,王翠妮,等.M1和M2型巨噬细胞表型的比较分析[J].现代免疫学,2008,28(3):177-183.
[11]阎永贞,那可,魏晓东,等.肿瘤免疫逃逸机制的研究进展[J].复旦学报(医学版),2013,40(5):619-624.
[12]常艳,魏伟.细胞共培养及其应用的研究进展[J].中国临床药理学与治疗学,2009,14(7):827-832.
[13]傅修涛.巨噬细胞在肝癌细胞上皮—间质样表型转化中的作用及其机制的研究[D].上海:复旦大学,2012.
[14]张驰.共培养体系下巨噬细胞对胃癌干细胞影响的研究[D].大连:大连医科大学,2016.
[2] CHEN W, ZHENG R, BAADE P D, et al. Cancer statistics in China, 2015[J]. CA Cancer J Clin, 2016, 66(2):115-132.
[3]洪琳,邵清松,周爱存,等.金线莲产业现状及可持续发展对策[J].中国中药杂志,2016,41(3):553-558.
[4]郭慧慧,蒋元斌,林丛发,等.金线莲研究进展[J].福建农业科技,2016(03):55-59.
[5]吴丽丽,梁燕,许光辉.金线莲化学成分、药理作用及临床应用研究概述[J].海峡药学,2014(10):34-37.
[6] GETACHEW Y, CUSIMANO F A, JAMES L P, et al. The role of intrahepatic CD3+/CD4-/CD8-double negative T(DN T)cells in enhanced acetaminophen toxicity[J]. Toxicology&Applied Pharmacology, 2014, 280(2):264-271.
[7]张多强,辛国军,丁洋,等.枸杞多糖对HepG2肝癌细胞自噬与细胞凋亡的影响[J].中成药,2017(12):2600-2602.
[8]刘栩岑,李玉洁,王娅杰,等.参莲提取物对PM(2.5)染毒RAW264.7细胞损伤的保护作用[J].中国中药杂志,2015,40(10):1977-1983.
[9]代艳文,袁丁,万静枝,等.竹节参总皂苷通过NF-κB通路对LPS致RAW264.7细胞炎症的保护作用研究[J].中国中药杂志,2014,39(11):2076.
[10]李康,郭强,王翠妮,等.M1和M2型巨噬细胞表型的比较分析[J].现代免疫学,2008,28(3):177-183.
[11]阎永贞,那可,魏晓东,等.肿瘤免疫逃逸机制的研究进展[J].复旦学报(医学版),2013,40(5):619-624.
[12]常艳,魏伟.细胞共培养及其应用的研究进展[J].中国临床药理学与治疗学,2009,14(7):827-832.
[13]傅修涛.巨噬细胞在肝癌细胞上皮—间质样表型转化中的作用及其机制的研究[D].上海:复旦大学,2012.
[14]张驰.共培养体系下巨噬细胞对胃癌干细胞影响的研究[D].大连:大连医科大学,2016.