艾灸、电针足三里对腓总神经横断术后大鼠胃黏膜损伤修复的影响
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摘要
目的探讨艾灸、电针启动相关内源性保护信息对胃黏膜的保护效应以及与腓总神经通路的关系。方法将72只SD大鼠随机分为正常组(A组)、模型组(B组)、模型艾灸组(C组)、手术模型艾灸组(D组)、模型电针组(E组)和手术模型电针组(F组),每组12只。除正常组外,其余各组均用无水乙醇、阿司匹林灌胃制备胃黏膜损伤模型,其中D组和F组在行腓总神经切断术后进行造模。C组和D组采用艾灸双侧足三里穴治疗,E组和F组采用电针双侧足三里穴治疗。治疗3 d后,采用苏木精-伊红(HE)染色观察各组大鼠胃黏膜形态学变化,计算胃黏膜溃疡指数(UI),并采用ELISA法检测各组大鼠血清与胃黏膜组织中一氧化氮(NO)、白细胞介素-1?(IL-1?)的含量。结果 B组、C组、D组、E组和F组UI、血清NO含量及胃黏膜组织中NO、IL-1?含量与A组比较,差异均具有统计学意义(P<0.01,P<0.05)。B组、D组、E组和F组血清IL-1?含量与A组比较,差异均具有统计学意义(P<0.01,P<0.05)。B组和D组UI、血清NO含量及胃黏膜组织中NO、IL-1?含量与C组比较,差异均具有统计学意义(P<0.01)。B组和F组UI、血清NO含量及胃黏膜组织中NO、IL-1?含量与E组比较,差异均具有统计学意义(P<0.01)。结论艾灸和电针足三里穴均可通过腓总神经途径传递信息,促使NO、IL-1?释放,从而有效促进胃黏膜修复,且两种方法治疗效果相近。
Objective To investigate the protective effect of moxibustion and electroacupuncture in initiating the relative endogenous protection information on gastric mucosa, and its relationship with the pathway of common peroneal nerve. Method Seventy-two Sprague-Dawley (SD) rats were randomized into a normal group (group A), a model group (group B), a moxibustion model group (group C), a moxibustion plus surgery model group (group D), an electroacupuncture model group (group E) and a electroacupuncture plus surgery model group (group F), with 12 rats in each group. Except for group A, rats in the other groups were treated with dehydrated ethanol and aspirin to prepare gastric mucosal lesion model. The rats in group D and group F were subjected to preparing the gastric mucosal damage model after the common peroneal nerve transection. Group C and group D were intervened by moxibustion at Zusanli (ST36), while Group E and group F were intervened by electroacupuncture at the same acupoints. After 3-day treatment, morphological changes in gastric mucosa of rats in each group were observed by hematoxylin-eosin (HE) staining, ulcer index (UI) was calculated, and nitric oxide (NO) and interleukin-1? (IL-1?) contents in serum and gastric mucosa tissue were examined by enzyme-linked immunosorbent assay (ELISA). Result The UI, NO content in serum, and NO and IL-1? contents in gastric mucosa tissue in group B, group C, group D, group E and group F were significantly different from those in group A (P<0.01, P<0.05). The IL-1? content in serum in group B, group D, group E and group F was significantly different from that in group A (P<0.01, P<0.05). The UI, NO content in serum, and NO and IL-1? contents in gastric mucosa tissue in group B and group D were significantly different from those in group C (P<0.01). The UI, NO content in serum, and NO and IL-1? contents in gastric mucosa tissue in group B and group F were significantly different from those of group E (P<0.01). Conclusion Both moxibustion and electroacupuncture at Zusanli (ST36) can transmit information through the pathway of common peroneal nerve, so as to promote the release of NO and IL-1?, which can effectively promote the recovery of gastric mucosa. The treatment efficacy of these two methods is similar.
Objective To investigate the protective effect of moxibustion and electroacupuncture in initiating the relative endogenous protection information on gastric mucosa, and its relationship with the pathway of common peroneal nerve. Method Seventy-two Sprague-Dawley (SD) rats were randomized into a normal group (group A), a model group (group B), a moxibustion model group (group C), a moxibustion plus surgery model group (group D), an electroacupuncture model group (group E) and a electroacupuncture plus surgery model group (group F), with 12 rats in each group. Except for group A, rats in the other groups were treated with dehydrated ethanol and aspirin to prepare gastric mucosal lesion model. The rats in group D and group F were subjected to preparing the gastric mucosal damage model after the common peroneal nerve transection. Group C and group D were intervened by moxibustion at Zusanli (ST36), while Group E and group F were intervened by electroacupuncture at the same acupoints. After 3-day treatment, morphological changes in gastric mucosa of rats in each group were observed by hematoxylin-eosin (HE) staining, ulcer index (UI) was calculated, and nitric oxide (NO) and interleukin-1? (IL-1?) contents in serum and gastric mucosa tissue were examined by enzyme-linked immunosorbent assay (ELISA). Result The UI, NO content in serum, and NO and IL-1? contents in gastric mucosa tissue in group B, group C, group D, group E and group F were significantly different from those in group A (P<0.01, P<0.05). The IL-1? content in serum in group B, group D, group E and group F was significantly different from that in group A (P<0.01, P<0.05). The UI, NO content in serum, and NO and IL-1? contents in gastric mucosa tissue in group B and group D were significantly different from those in group C (P<0.01). The UI, NO content in serum, and NO and IL-1? contents in gastric mucosa tissue in group B and group F were significantly different from those of group E (P<0.01). Conclusion Both moxibustion and electroacupuncture at Zusanli (ST36) can transmit information through the pathway of common peroneal nerve, so as to promote the release of NO and IL-1?, which can effectively promote the recovery of gastric mucosa. The treatment efficacy of these two methods is similar.
引文
[1]陈果,向娟,欧阳里知,等.艾灸刺激对腓总神经横断术后大鼠胃黏膜修复作用机制的影响[J].世界华人消化志,2016,24(2):248-254.
[2]粟艳梅,张泓,张雨辰,等.艾灸对脾虚胃溃疡模型大鼠MT及HSP 70影响[J].辽宁中医药大学学报,2015,17(1):80-83.
[3]刘密,常小荣,严洁,等.艾灸预处理对大鼠应激性胃黏膜损伤的保护作用[J].中华中医药杂志,2012,27(1):58-62.
[4]易受乡,彭艳,常小荣,等.艾灸足三里、梁门穴对应激性溃疡大鼠胃黏膜细胞凋亡的干预作用[J].世界华人消化杂志,2006,14(33):3163-3168.
[5]向娟,陈果,欧阳里知,等.艾灸“足三里”对胃黏膜损伤大鼠内源性保护因子含量及相关蛋白表达的影响[J].北京中医药大学学报,2016,39(5):406-412.
[6]黄碧兰,王秋桂,秦灵芝,等.外周神经参与电针足三里穴抗大鼠应激性胃粘膜损伤作用[J].中华医学与健康,2006,7(1):1-3.
[7]向娟,陈果,李飞,等.针灸治疗胃黏膜损伤中枢调控机制的研究[J].上海针灸杂志,2016,35(12):1484-1487.
[8]向丽婷,李飞,刘芳,等.从迷走神经通路研究艾灸对胃粘膜损伤修复机制的研究进展[J].针灸临床杂志,2016,32(7):84-87.
[9]El-Ashmawy NE,Khedr EG,El-Bahrawy HA,et al.Gastroprotective effect of garlic in indomethacin induced gastric ulcer in rats[J].Nutrition,2016,32(7-8):849-854.
[10]Akanda MR,Park BY.Involvement of MAPK/NF-κBsignal transduction pathways:Camellia japonica mitigates inflammation and gastric ulcer[J].Biomed Pharmacother,2017,95:1139-1146.
[11]Suárez J,Herrera MD,Marhuenda E.Hesperidin and neohesperidin dihydrochalcone on different experi-mental models of induced gastric ulcer[J].Phytotherapy Research,2015,10(7):616-618.
[12]陈志峰.探索理想的大鼠慢性胃溃疡模型的建立方法[D].汕头大学,2012:6-7.
[13]李忠仁.试验针灸学[M].北京:中国中医药出版社,2007:327-329.
[14]Guth PH,Aures D,Paulsen G.Topical aspirin plus HCl gastric lesions in the rat.Cytoprotective effect of prostaglandin,cimetidine,and probanthine[J].Gastroenterology,1979,76(1):88-93.
[15]Repetto MG,Boveris A.Bioactivity of sesquiterpenes:compounds that protect from alcohol-induced gastric mucosal lesions and oxidative damage[J].Mini Rev Med Chem,2010,10(7):615-623.
[16]郭礼娜,刘薇薇,谭成富,等.艾灸、电针预处理对急性胃黏膜损伤大鼠TGF-?、PCNA的影响[J].湖南中医药大学学报,2016,36(4):45-48.
[17]李铁浪,粟艳梅,祁芳,等.艾灸对脾虚胃溃疡模型大鼠血清TFF及胃黏膜ERK1/2、PCNA的影响[J].湖南中医药大学学报,2015,35(2):49-51.
[18]杨宗保,王亚东,刘琼,等.电针胃经穴抑制胃溃疡大鼠氧化损伤的作用机制研究[J].中国针灸,2016,36(6):617-621.
[19]彭亮.艾灸预处理对神经通路切断/损毁术后大鼠胃黏膜保护作用机制的研究[D].湖南中医药大学,2012:4-5.
[20]Mori H,Kobara H,Rahman A,et al.Innovative delivery method using a detachable device to deliver a large polyglycolic acid sheet to a gastric ulcer perforation[J].Endoscopy,2017,49(7):E165-E167.
[21]Zhao Z,Gong S,Wang S,et al.Effect and mechanism of evodiamine against ethanol-induced gastric ulcer in mice by suppressing Rho/NF-кB pathway[J].Int Immunophar-macol,2015,28(1):588-595.
[22]张海云,常香荣.CIP2A过表达对胃黏膜上皮细胞增殖和凋亡的影响[J].中国病理生理杂志,2016,32(10):1881-1886.
[23]邵雪辉,王建国,张晓丽,等.激光照射足三里穴对慢性萎缩性胃炎大鼠胃酸分泌及NO含量的影响[J].中华物理医学与康复杂志,2016,38(7):492-496.
[24]刘密,常小荣,严洁,等.艾灸预处理对应激性胃黏膜损伤大鼠血清的IL-1?、TNF-?和IL-10影响[J].中国中医急症,2011,20(6):906-908.
[2]粟艳梅,张泓,张雨辰,等.艾灸对脾虚胃溃疡模型大鼠MT及HSP 70影响[J].辽宁中医药大学学报,2015,17(1):80-83.
[3]刘密,常小荣,严洁,等.艾灸预处理对大鼠应激性胃黏膜损伤的保护作用[J].中华中医药杂志,2012,27(1):58-62.
[4]易受乡,彭艳,常小荣,等.艾灸足三里、梁门穴对应激性溃疡大鼠胃黏膜细胞凋亡的干预作用[J].世界华人消化杂志,2006,14(33):3163-3168.
[5]向娟,陈果,欧阳里知,等.艾灸“足三里”对胃黏膜损伤大鼠内源性保护因子含量及相关蛋白表达的影响[J].北京中医药大学学报,2016,39(5):406-412.
[6]黄碧兰,王秋桂,秦灵芝,等.外周神经参与电针足三里穴抗大鼠应激性胃粘膜损伤作用[J].中华医学与健康,2006,7(1):1-3.
[7]向娟,陈果,李飞,等.针灸治疗胃黏膜损伤中枢调控机制的研究[J].上海针灸杂志,2016,35(12):1484-1487.
[8]向丽婷,李飞,刘芳,等.从迷走神经通路研究艾灸对胃粘膜损伤修复机制的研究进展[J].针灸临床杂志,2016,32(7):84-87.
[9]El-Ashmawy NE,Khedr EG,El-Bahrawy HA,et al.Gastroprotective effect of garlic in indomethacin induced gastric ulcer in rats[J].Nutrition,2016,32(7-8):849-854.
[10]Akanda MR,Park BY.Involvement of MAPK/NF-κBsignal transduction pathways:Camellia japonica mitigates inflammation and gastric ulcer[J].Biomed Pharmacother,2017,95:1139-1146.
[11]Suárez J,Herrera MD,Marhuenda E.Hesperidin and neohesperidin dihydrochalcone on different experi-mental models of induced gastric ulcer[J].Phytotherapy Research,2015,10(7):616-618.
[12]陈志峰.探索理想的大鼠慢性胃溃疡模型的建立方法[D].汕头大学,2012:6-7.
[13]李忠仁.试验针灸学[M].北京:中国中医药出版社,2007:327-329.
[14]Guth PH,Aures D,Paulsen G.Topical aspirin plus HCl gastric lesions in the rat.Cytoprotective effect of prostaglandin,cimetidine,and probanthine[J].Gastroenterology,1979,76(1):88-93.
[15]Repetto MG,Boveris A.Bioactivity of sesquiterpenes:compounds that protect from alcohol-induced gastric mucosal lesions and oxidative damage[J].Mini Rev Med Chem,2010,10(7):615-623.
[16]郭礼娜,刘薇薇,谭成富,等.艾灸、电针预处理对急性胃黏膜损伤大鼠TGF-?、PCNA的影响[J].湖南中医药大学学报,2016,36(4):45-48.
[17]李铁浪,粟艳梅,祁芳,等.艾灸对脾虚胃溃疡模型大鼠血清TFF及胃黏膜ERK1/2、PCNA的影响[J].湖南中医药大学学报,2015,35(2):49-51.
[18]杨宗保,王亚东,刘琼,等.电针胃经穴抑制胃溃疡大鼠氧化损伤的作用机制研究[J].中国针灸,2016,36(6):617-621.
[19]彭亮.艾灸预处理对神经通路切断/损毁术后大鼠胃黏膜保护作用机制的研究[D].湖南中医药大学,2012:4-5.
[20]Mori H,Kobara H,Rahman A,et al.Innovative delivery method using a detachable device to deliver a large polyglycolic acid sheet to a gastric ulcer perforation[J].Endoscopy,2017,49(7):E165-E167.
[21]Zhao Z,Gong S,Wang S,et al.Effect and mechanism of evodiamine against ethanol-induced gastric ulcer in mice by suppressing Rho/NF-кB pathway[J].Int Immunophar-macol,2015,28(1):588-595.
[22]张海云,常香荣.CIP2A过表达对胃黏膜上皮细胞增殖和凋亡的影响[J].中国病理生理杂志,2016,32(10):1881-1886.
[23]邵雪辉,王建国,张晓丽,等.激光照射足三里穴对慢性萎缩性胃炎大鼠胃酸分泌及NO含量的影响[J].中华物理医学与康复杂志,2016,38(7):492-496.
[24]刘密,常小荣,严洁,等.艾灸预处理对应激性胃黏膜损伤大鼠血清的IL-1?、TNF-?和IL-10影响[J].中国中医急症,2011,20(6):906-908.