应用微卫星DNA标记对广西食蟹猴的遗传学研究
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摘要
目的对广西食蟹猴不同生产繁殖群进行遗传检测,分析广西不同繁殖群食蟹猴的遗传背景,为建立广西食蟹猴遗传质量检测方法和种群资源库提供基础资料。方法应用20个微卫星基因位点和毛细管电泳技术对广西3个不同生产群食蟹猴进行遗传检测,并计算群体内和群体间的遗传变异参数。结果共检测到等位基因237个,其观察等位基因数(Na)为5～19个,平均11.85个;平均期望杂合度(He)为0.85;平均多态信息含量(PIC)为0.817。3个不同生产群(F、N和W)分别检测到等位基因158、158和173个,平均期望杂合度(He)分别为0.8371、0.8318和0.8642;平均多态信息含量(PIC)分别为0.7692、0.7653和0.8001,3个不同生产群存在较高的遗传多态性。3个生产群Hardy-Weinberg平衡检验多数位点处于H-W平衡。群内近交系数Fis均值为0.0235,总群体近交系数Fit均值为0.0628,遗传分化系数Fst均值为0.0402,基因流Nm均值为5.9616,表明3个生产群基本处于随机交配状态,群体间遗传分化很小。3个生产群遗传距离为0.2556～0.3223,遗传相似度为0.7245～0.7745,聚类分析显示F群体和N群体先聚为一类,再和W群体聚为一类,符合3个繁殖猴场各自关联引种历史特征。结论本研究有效分析了广西食蟹猴的遗传多态性和群体间的遗传关系,所选的20个微卫星基因位点可用于食蟹猴遗传质量检测。
Objective To genetic monitoring of Guangxi Macaca fascicularis from different breeding groups, and analyze their genetic background, provide basic information for establishing the genetic quality monitoring method and population repository of Guangxi Macaca fascicularis.Method Twenty microsatellite DNA markers and capillary electrophoresis were adopted to genetic detect Guangxi Macaca fascicularis from three different breeding groups, and the genetic variation parameters within and between populations were calculated.Result A total of 237 alleles were detected in the Guangxi Macaca fascicularis, the observed allelic number was ranging from 5 to 19, with a mean of 11.85. The mean expected heterozygosity was 0.85, and the mean polymorphism information content was 0.817. In the three different breeding groups(F,N and W), respectively, 158, 158 and 173 alleles were detected, the mean expected heterozygosity was 0.8371, 0.8318 and 0.8642, and the mean polymorphism information content was 0.7692, 0.7653 and 0.8001. The three breeding groups showed the high genetic diversity. The Hardy-Weinberg equilibrium test for 3 breeding groups showed that most loci were in H-W equilibrium. Respectively, the average Fis, Fit, Fst of all loci was 0.0235, 0.0628, 0.0402, the index of gene flow was 5.9616, which implied that 3 breeding groups was nearly under the random mating system and had low genetic differentiation. The genetic distance was ranging from 0.2556 to 0.3223, the genetic similarity was 0.7245 to 0.7745. Cluster analysis showed that F population first clustered with N population, after that clustered with W population, it is conformed to their historical introduction.Conclusion The study effectively analyzed the genetic diversity and genetic relationship of Guangxi Macaca fascicularis, and the 20 microsatellite DNA markers selected in this study could be used to detect genetic quality of Macaca fascicularis.
Objective To genetic monitoring of Guangxi Macaca fascicularis from different breeding groups, and analyze their genetic background, provide basic information for establishing the genetic quality monitoring method and population repository of Guangxi Macaca fascicularis.Method Twenty microsatellite DNA markers and capillary electrophoresis were adopted to genetic detect Guangxi Macaca fascicularis from three different breeding groups, and the genetic variation parameters within and between populations were calculated.Result A total of 237 alleles were detected in the Guangxi Macaca fascicularis, the observed allelic number was ranging from 5 to 19, with a mean of 11.85. The mean expected heterozygosity was 0.85, and the mean polymorphism information content was 0.817. In the three different breeding groups(F,N and W), respectively, 158, 158 and 173 alleles were detected, the mean expected heterozygosity was 0.8371, 0.8318 and 0.8642, and the mean polymorphism information content was 0.7692, 0.7653 and 0.8001. The three breeding groups showed the high genetic diversity. The Hardy-Weinberg equilibrium test for 3 breeding groups showed that most loci were in H-W equilibrium. Respectively, the average Fis, Fit, Fst of all loci was 0.0235, 0.0628, 0.0402, the index of gene flow was 5.9616, which implied that 3 breeding groups was nearly under the random mating system and had low genetic differentiation. The genetic distance was ranging from 0.2556 to 0.3223, the genetic similarity was 0.7245 to 0.7745. Cluster analysis showed that F population first clustered with N population, after that clustered with W population, it is conformed to their historical introduction.Conclusion The study effectively analyzed the genetic diversity and genetic relationship of Guangxi Macaca fascicularis, and the 20 microsatellite DNA markers selected in this study could be used to detect genetic quality of Macaca fascicularis.
引文
[1] 李瑞生,李晓娟,高蓉,等.食蟹猴微卫星DNA标记遗传监测及多态性分析[J].中国比较医学杂志,2011,21(8):27-30.
[2] 江鹏亮,汤球,余琛琳,等.非人灵长类动物模型在医学研究中的应用概况[J].实验动物科学,2010,27(6):59-64.
[3] 高建峰,滕利,周立,等.恒河猴和食蟹猴在结核病研究中的应用进展[J].实验动物科学,2014,31(6):51-55.
[4] 季芳,饶军华,刘晓明.应用微卫星标记对海南和广西恒河猴遗传多样性的研究[J].实验动物科学与管理,2006,23(2):26-30.
[5] 张卉,李进华,赵健元,等.皖南山区猕猴种群的形态特征与微卫星遗传多样性初步分析[J].实验动物与比较医学,2008,28(4):225-229.
[6] 李瑞生,赵爽,高蓉,等.恒河猴与食蟹猴微卫星DNA多态性的比较分析[J].中国实验动物学报,2009,17(1):57-60.
[7] 周建华,李志雄,杨燕燕,等.基于微卫星DNA标记分析福建猕猴与河南猕猴的遗传多样性[J].福建中医药,2017,48(5):30-34.
[8] 刘欢.我国笼养食蟹猴种群遗传多样性与亲子鉴定研究[D].哈尔滨:东北林业大学,2010:1-43.
[9] 瞿陆峰,潘伟荣,曾养志.微卫星DNA标记及其应用[J].畜牧与饲料科学,2010,31(4):6-8.
[10] Kikuchi T,Hara M,Terao K.Development of a microsatellite marker set applicable to genome-wide screening of cynomolgus monkeys(Macaca fascieularis)[J].Primates,2007,48(2):140-146.
[11] Nikzad S,Tan S G,Yong S Y C,et al.Genetic diversity and population structure of long-tailed macaque (Macaca fascicularis) populations in Peninsular Malaysia[J].J Med Primatol,2014,43(6):433-444.
[12] Smith D G,Ng J,George D,et al.A genetic comparison of two alleged subspecies of Philippine cynomolgus macaques[J].Am J Phys Anthropol,2014,155(1):136-148.
[13] 李芳芳,魏杰,王洪,等.应用微卫星标记对两个豚鼠封闭群的遗传学研究[J].中国比较医学杂志,2014,24(12):33-38+46.
[14] 皮道元,谢莉萍,卢晟盛,等.利用微卫星标记分析4个食蟹猴群体的遗传多样性[J].中国兽医学报,2011,31(3):444-448.
[15] Wright S.Evolution and the Genetics of Populations Variability within and among Natural Populations [M].1st edn.Chicago,University of Chicago Press,1978,395-486.
[16] 曲若竹,侯林,吕红丽,等.群体遗传结构中的基因流[J] .遗传,2004,26(3):377-382.
[2] 江鹏亮,汤球,余琛琳,等.非人灵长类动物模型在医学研究中的应用概况[J].实验动物科学,2010,27(6):59-64.
[3] 高建峰,滕利,周立,等.恒河猴和食蟹猴在结核病研究中的应用进展[J].实验动物科学,2014,31(6):51-55.
[4] 季芳,饶军华,刘晓明.应用微卫星标记对海南和广西恒河猴遗传多样性的研究[J].实验动物科学与管理,2006,23(2):26-30.
[5] 张卉,李进华,赵健元,等.皖南山区猕猴种群的形态特征与微卫星遗传多样性初步分析[J].实验动物与比较医学,2008,28(4):225-229.
[6] 李瑞生,赵爽,高蓉,等.恒河猴与食蟹猴微卫星DNA多态性的比较分析[J].中国实验动物学报,2009,17(1):57-60.
[7] 周建华,李志雄,杨燕燕,等.基于微卫星DNA标记分析福建猕猴与河南猕猴的遗传多样性[J].福建中医药,2017,48(5):30-34.
[8] 刘欢.我国笼养食蟹猴种群遗传多样性与亲子鉴定研究[D].哈尔滨:东北林业大学,2010:1-43.
[9] 瞿陆峰,潘伟荣,曾养志.微卫星DNA标记及其应用[J].畜牧与饲料科学,2010,31(4):6-8.
[10] Kikuchi T,Hara M,Terao K.Development of a microsatellite marker set applicable to genome-wide screening of cynomolgus monkeys(Macaca fascieularis)[J].Primates,2007,48(2):140-146.
[11] Nikzad S,Tan S G,Yong S Y C,et al.Genetic diversity and population structure of long-tailed macaque (Macaca fascicularis) populations in Peninsular Malaysia[J].J Med Primatol,2014,43(6):433-444.
[12] Smith D G,Ng J,George D,et al.A genetic comparison of two alleged subspecies of Philippine cynomolgus macaques[J].Am J Phys Anthropol,2014,155(1):136-148.
[13] 李芳芳,魏杰,王洪,等.应用微卫星标记对两个豚鼠封闭群的遗传学研究[J].中国比较医学杂志,2014,24(12):33-38+46.
[14] 皮道元,谢莉萍,卢晟盛,等.利用微卫星标记分析4个食蟹猴群体的遗传多样性[J].中国兽医学报,2011,31(3):444-448.
[15] Wright S.Evolution and the Genetics of Populations Variability within and among Natural Populations [M].1st edn.Chicago,University of Chicago Press,1978,395-486.
[16] 曲若竹,侯林,吕红丽,等.群体遗传结构中的基因流[J] .遗传,2004,26(3):377-382.