HPLC-DAD法同时测定蒙药森登-4汤散中7种抗风湿活性成分的含量
|
摘要
目的:建立同时测定蒙药森登-4汤散中7种抗风湿活性成分儿茶素、栀子苷、二氢杨梅素、花旗松素、芦丁、杨梅素和槲皮素含量的方法。方法:采用高效液相色谱-二极管阵列检测法(HPLC-DAD)。色谱柱为Sil Green C_(18),流动相为乙腈-0.1%磷酸溶液(梯度洗脱),流速为1 mL/min,检测波长为238 nm,柱温为30℃,进样量为10μL。结果:儿茶素、栀子苷、二氢杨梅素、花旗松素、芦丁、杨梅素和槲皮素检测质量浓度的线性范围分别为8.590～2 290、10.56～3 901、13.00～3 958、8.815～564.2、4.030～257.8、8.130～750.5、7.075～454.2μg/mL(r≥0.999 1);检测限分别为0.429 5、0.264 0、0.325 0、0.220 4、0.201 5、0.203 2、0.176 9μg/mL,定量限分别为1.030、1.321、1.302、1.397、1.637、0.813 0、0.707 5μg/mL;精密度、稳定性(48 h)、重复性试验的RSD均小于2.0%(n=6);平均加样回收率分别为96.24%～99.28%,RSD分别为1.03%～1.63%(n=6)。结论:该方法简便、准确、重复性良好,可用于同时测定蒙药森登-4汤散中儿茶素、栀子苷、二氢杨梅素、花旗松素、芦丁、杨梅素和槲皮素的含量。
OBJECTIVE:To establish a method for simultaneous determination of 7 kinds of anti-rheumatic active ingredients in Mongolian medicine Sendeng-4 decoction powder,such as catechin,jasminoidin,dihydromyricetin,texifolin,rutin,myricetin and quercetin. METHODS:HPLC-DAD method was adopted. The determination was performed on Sil Green C_(18) column with mobile phase consisted of acetonitrile-0.1% phosphoric acid solution(gradient elution)at the flow rate of 1 mL/min. The detection wavelength was set at 238 nm,and column temperature was 30 ℃. The sample size was 10 μL. RESULTS:The linear range of catechin,jasminoidin,dihydromyricetin,texifolin,rutin,myricetin and quercetin were 8.590-2 290,10.56-3 901,13.00-3 958,8.815-564.2,4.030-257.8,8.130-750.5,7.075-454.2 μ g/mL(r≥0.999 1),respectively;the limits of detection were 0.429 5,0.264 0,0.325 0,0.220 4,0.201 5,0.203 2,0.176 9 μg/mL,respectively;the limits of quantification were 1.030,1.321,1.302,1.397,1.637,0.813 0,0.707 5 μg/mL,respectively. RSDs of precision,stability(48 h)and repetition tests were all lower than2.0%(n=6). The average recoveries were 96.24%-99.28%(RSD=1.03%-1.63%,n=6). CONCLUSIONS:The established method is simple, accurate and reproducible, and can be used for simultaneous determination of catechin, jasminoidin,dihydromyricetin,texifolin,rutin,myricetin and quercetin in Mongolian medicine Sendeng-4 decoction powder.
OBJECTIVE:To establish a method for simultaneous determination of 7 kinds of anti-rheumatic active ingredients in Mongolian medicine Sendeng-4 decoction powder,such as catechin,jasminoidin,dihydromyricetin,texifolin,rutin,myricetin and quercetin. METHODS:HPLC-DAD method was adopted. The determination was performed on Sil Green C_(18) column with mobile phase consisted of acetonitrile-0.1% phosphoric acid solution(gradient elution)at the flow rate of 1 mL/min. The detection wavelength was set at 238 nm,and column temperature was 30 ℃. The sample size was 10 μL. RESULTS:The linear range of catechin,jasminoidin,dihydromyricetin,texifolin,rutin,myricetin and quercetin were 8.590-2 290,10.56-3 901,13.00-3 958,8.815-564.2,4.030-257.8,8.130-750.5,7.075-454.2 μ g/mL(r≥0.999 1),respectively;the limits of detection were 0.429 5,0.264 0,0.325 0,0.220 4,0.201 5,0.203 2,0.176 9 μg/mL,respectively;the limits of quantification were 1.030,1.321,1.302,1.397,1.637,0.813 0,0.707 5 μg/mL,respectively. RSDs of precision,stability(48 h)and repetition tests were all lower than2.0%(n=6). The average recoveries were 96.24%-99.28%(RSD=1.03%-1.63%,n=6). CONCLUSIONS:The established method is simple, accurate and reproducible, and can be used for simultaneous determination of catechin, jasminoidin,dihydromyricetin,texifolin,rutin,myricetin and quercetin in Mongolian medicine Sendeng-4 decoction powder.
引文
[1]吉格木德丹金扎木苏.观者之喜[M].敖特根毕力格,段关布扎布,仁庆稍,译.赤峰:内蒙古科学技术出版社,2015:209.
[2]布和巴特尔,奥·乌力吉.传统蒙药与方剂[M].赤峰:内蒙古科学技术出版社,2013:213.
[3]XU L,ZHANG L,LI Q,et al.Determination of geniposide in rats plasma.Application to pharmacokinetic studies of sendeng-4 decoction[J].Chromatographia,2006,63(9/10):493-497.
[4]许良,毕开顺.多元线性回归分析法在蒙药森登-4汤谱效关系解析中的应用[J].计算机与应用化学,2008,25(10):1189-1192.
[5]李丹.基于组学方法及LC-MS技术的两种蒙药(森登-4和丹参)作用机制研究[D].通辽:内蒙古民族大学,2016.
[6]WANG X,LI D,JIANG M,et al.K Nearest Neighbor algorithm coupled with metabonomics to study the therapeutic mechanism of Sendeng-4 in adjuvant-induced rheumatoid arthritis rat[J].Evid Based Complement Alternat Med,2018.DOI:10.1155/2018/2484912.
[7]王曦烨,李丹,唐兴盟,等.基于组学方法的蒙药复方森登-4配伍机制研究[J].中草药,2016,47(5):736-740.
[8]唐兴盟,刘景林,许良.蒙药复方森登-4汤现代研究若干进展[J].内蒙古民族大学学报(自然科学版),2015,30(5):420-423.
[9]赵娅,冯慧,周珍,等.HPLC法同时测定四味姜黄汤散中7种成分的含量[J].中国药房,2018,29(1):29-33.
[10]陈雁,张现涛,张雷红,等.栀子化学成分及药理作用研究进展[J].海峡药学,2010,22(12):1-5.
[11]杨小娟,高晓黎.文冠果化学成分及药理作用研究进展[J].西北药学杂志,2004,19(5):235-237.
[12]徐先祥.儿茶素的药理作用研究综述[J].郑州轻工业学院学报(自然科学版),2012,27(4):60-64.
[13]林国钡,谢燕,李国文.杨梅素的研究进展[J].国际药学研究杂志,2012,39(6):483-487.
[14]侯小龙,王文清,施春阳,等.二氢杨梅素药理作用研究进展[J].中草药,2015,46(4):603-609.
[15]乔华,谢鋆,张晓云.花旗松素的生物活性及其应用[J].中草药,2003,34(8):15-17.
[16]马开,田萍,张薇,等.青钱柳叶HPLC指纹图谱研究及9个成分定量分析[J].中药材,2018,41(8):1904-1909.
[2]布和巴特尔,奥·乌力吉.传统蒙药与方剂[M].赤峰:内蒙古科学技术出版社,2013:213.
[3]XU L,ZHANG L,LI Q,et al.Determination of geniposide in rats plasma.Application to pharmacokinetic studies of sendeng-4 decoction[J].Chromatographia,2006,63(9/10):493-497.
[4]许良,毕开顺.多元线性回归分析法在蒙药森登-4汤谱效关系解析中的应用[J].计算机与应用化学,2008,25(10):1189-1192.
[5]李丹.基于组学方法及LC-MS技术的两种蒙药(森登-4和丹参)作用机制研究[D].通辽:内蒙古民族大学,2016.
[6]WANG X,LI D,JIANG M,et al.K Nearest Neighbor algorithm coupled with metabonomics to study the therapeutic mechanism of Sendeng-4 in adjuvant-induced rheumatoid arthritis rat[J].Evid Based Complement Alternat Med,2018.DOI:10.1155/2018/2484912.
[7]王曦烨,李丹,唐兴盟,等.基于组学方法的蒙药复方森登-4配伍机制研究[J].中草药,2016,47(5):736-740.
[8]唐兴盟,刘景林,许良.蒙药复方森登-4汤现代研究若干进展[J].内蒙古民族大学学报(自然科学版),2015,30(5):420-423.
[9]赵娅,冯慧,周珍,等.HPLC法同时测定四味姜黄汤散中7种成分的含量[J].中国药房,2018,29(1):29-33.
[10]陈雁,张现涛,张雷红,等.栀子化学成分及药理作用研究进展[J].海峡药学,2010,22(12):1-5.
[11]杨小娟,高晓黎.文冠果化学成分及药理作用研究进展[J].西北药学杂志,2004,19(5):235-237.
[12]徐先祥.儿茶素的药理作用研究综述[J].郑州轻工业学院学报(自然科学版),2012,27(4):60-64.
[13]林国钡,谢燕,李国文.杨梅素的研究进展[J].国际药学研究杂志,2012,39(6):483-487.
[14]侯小龙,王文清,施春阳,等.二氢杨梅素药理作用研究进展[J].中草药,2015,46(4):603-609.
[15]乔华,谢鋆,张晓云.花旗松素的生物活性及其应用[J].中草药,2003,34(8):15-17.
[16]马开,田萍,张薇,等.青钱柳叶HPLC指纹图谱研究及9个成分定量分析[J].中药材,2018,41(8):1904-1909.