表皮生长因子冻干粉对敏感性皮肤屏障功能改善作用的临床观察
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摘要
目的评价外用含有表皮生长因子(EGF)的护肤品对敏感性皮肤生理功能的影响及不良反应。方法首先受试者接受斑贴试验检测接触过敏情况,对30例敏感性皮肤受试者面部外用含有EGF的护肤品,受试者早晚洁面后按推荐剂量(0.4～0.5 mL)每日2次,连续使用28 d。在治疗前及治疗后7、14、21、28 d分别对受试者皮肤生理功能进行检测并做乳酸试验,同时对受试者皮损改善情况及安全性进行评价。结果 30例斑贴试验结果均为阳性,阳性项目前3位的是硫柳汞、芳香混合物、重铬酸钾。与治疗前相比,治疗后14 d患者面部含水量、油脂含量开始呈增加趋势,pH值呈减小趋势(P<0.05);而经皮水流失情况在治疗后21 d,乳酸试验和皮损改善评分在治疗7 d起呈减少趋势(P<0.05)。治疗前,治疗后7、14、21、28 d的阳性率呈逐渐降低趋势(P<0.001),28 d后乳酸刺激试验仍为阳性的7例受试者中2例为硫酸镍(++)、2例1项(+)、1例4项(+)、2例5项(+)。皮损改善率呈增加趋势(P=0.058),斑贴试验示治疗28 d皮损仍无明显改善者的4例中2例5项(+)、2例4项(+)。治疗前后均未发生不良反应,无一例患者脱落。结论含有EGF的护肤产品有效、安全且有助于恢复敏感皮肤患者的皮肤屏障功能。
Objective To evaluate the effects and adverse reactions of external skin care products containing epidermal growth factor(EGF) on physiological functions of sensitive skin. Methods First, 30 patients with sensitive skin received patch test to detect contact allergy. The external skin care products containing EGF were used twice a day for 28 days according to recommended dose(0.4-0.5 mL). A non-invasive skin test was done before the trial and on the day 7, 14,21 and 28 during the trial to evaluate the physiological function of skin. The lactic acid, the recovery of skin lesions and safety of the product were evaluated at the same time. Results The results of 30 patch tests were positive. The water content of the stratum corneum and the oil content of the skin began to increase at 14 days after treatment, and the pH value decreased(P < 0.05). The transepidermal water loss(TEWL), lactic acid test and the skin lesion improvement score showed a decreasing trend at 21 d, 7 d and 7 d, respectively(P < 0.05). The positive rates showed a decreasing trend after 7 d, 14 d, 21 d and 28 d treatment(P < 0.001). Seven subjects whose lactic acid stimulation test were still positive after 28 d included 2 cases of nickel(++), 2 cases of 1 tested item(+), 1 case of 4 tested items(+) and 2 cases of 5 tested items(+). The improvement rate of skin lesions showed an increasing trend(P=0.058). There were 2 cases with 5 tested items(+) and 2 cases with 4 tested items(+) in 4 subjects with no significant improvement in skin lesions for 28 days. No side effects occurred before and after treatment, and none of the patients fell off. Conclusion Skin care products containing oligopeptide-1(EGF) are effective, safe and helpful for recovering the skin barrier function in patients with sensitive skin.
Objective To evaluate the effects and adverse reactions of external skin care products containing epidermal growth factor(EGF) on physiological functions of sensitive skin. Methods First, 30 patients with sensitive skin received patch test to detect contact allergy. The external skin care products containing EGF were used twice a day for 28 days according to recommended dose(0.4-0.5 mL). A non-invasive skin test was done before the trial and on the day 7, 14,21 and 28 during the trial to evaluate the physiological function of skin. The lactic acid, the recovery of skin lesions and safety of the product were evaluated at the same time. Results The results of 30 patch tests were positive. The water content of the stratum corneum and the oil content of the skin began to increase at 14 days after treatment, and the pH value decreased(P < 0.05). The transepidermal water loss(TEWL), lactic acid test and the skin lesion improvement score showed a decreasing trend at 21 d, 7 d and 7 d, respectively(P < 0.05). The positive rates showed a decreasing trend after 7 d, 14 d, 21 d and 28 d treatment(P < 0.001). Seven subjects whose lactic acid stimulation test were still positive after 28 d included 2 cases of nickel(++), 2 cases of 1 tested item(+), 1 case of 4 tested items(+) and 2 cases of 5 tested items(+). The improvement rate of skin lesions showed an increasing trend(P=0.058). There were 2 cases with 5 tested items(+) and 2 cases with 4 tested items(+) in 4 subjects with no significant improvement in skin lesions for 28 days. No side effects occurred before and after treatment, and none of the patients fell off. Conclusion Skin care products containing oligopeptide-1(EGF) are effective, safe and helpful for recovering the skin barrier function in patients with sensitive skin.
引文
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[8] Zhang Z,Xiao C,Gibson AM,et al. EGFR signaling bluntsallergen-induced IL-6 production and Th17 responses in the skinand attenuates development and relapse of atopic dermatitis[J]. JImmunol, 2014, 192(3):859-866. doi:10.4049/jimmunol.1301062.
[9] Kim JM,Choo JE,Lee HJ,et al. Epidermal growth factor attenuatedthe expression of inflammatory cytokines in human epidermalkeratinocyte exposed to propionibacterium acnes[J]. AnnDermatol,2018,30(1):54-63. doi:10.5021/ad.2018.30.1.54.
[10]Hoi SY,Lee YJ,Kim JM,et al. Epidermal growth factor relievesinflammatory signals in staphylococcus aureus-treated humanepidermal keratinocytes and atopic dermatitis-like skin lesions inNc/Nga Mice[J]. Biomed Res Int,2018,2018:9439182. doi:10.1155/2018/9439182.
[11]张秀丽,张理涛. rhEGF凝胶对面部激素依赖性皮炎皮肤功能改善的临床研究[J].天津医药,2016,45(5):629-631.ZhangXL,Zhang LT. Clinical study on the efficiency of rhEGF gel to skinfunction of facial hormone-dependent dermatitis[J]. Tianjin Med J,2016,45(5):629-631. doi:10.11958/20150434.
[2] Yamaoka T,Ohba M,Ohmori T. Molecular-targeted therapies forepidermal growth factor receptor and its resistance mechanisms[J].Int J Mol Sci,2017,18(11). pii:E2420. doi:10.3390/ijms18112420.
[3]李邻峰,顾恒.斑贴试验临床应用专家共识[J].中华皮肤科杂志,2015,48(1):8-10. Committee of allergy and clinicalimmunology, dermatovenerology branch, Chinese medicalassociation. Expert consensus on clinical application of patch test[J]. Chin J dermatol, 2015,48(1):8-10. doi:10.3760/cma. j.issn.0412-4030.2015.01.004.
[4] Berardesca E,Farage M,Maibach H. Sensitive skin:an overview[J]. Int J Cosmet Sci,2013,35(1):2-8. doi:10.1111/j. 1468-2494.2012.00754.x.
[5] Richters RJ,Uzunbajakava NE,Falcone D,et al. Clinical,biophysical and immunohistochemical analysis of skin reactions toacute skin barrier disruption-a comparative trial betweenparticipants with sensitive skin and those with nonsensitive skin[J]. Br J Dermatol,2016,174(5):1126-1133. doi:10.1111/bjd.14307.
[6] den Hollander L,Han H,de Winter M,et al. Skin lamellar bodiesare not discrete vesicles but part of a tubuloreticular network[J].Acta Derm Venereol,2016,96(3):303-308. doi:10.2340/00015555-2249.
[7] An S,Lee E,Kim S,et al. Comparison and correlation betweenstinging responses to lactic acid and bioengineering parameters[J].Contact Dermatitis,2007,57(3):158-162. doi:10.1111/j.1600-0536.2007.01182.x.
[8] Zhang Z,Xiao C,Gibson AM,et al. EGFR signaling bluntsallergen-induced IL-6 production and Th17 responses in the skinand attenuates development and relapse of atopic dermatitis[J]. JImmunol, 2014, 192(3):859-866. doi:10.4049/jimmunol.1301062.
[9] Kim JM,Choo JE,Lee HJ,et al. Epidermal growth factor attenuatedthe expression of inflammatory cytokines in human epidermalkeratinocyte exposed to propionibacterium acnes[J]. AnnDermatol,2018,30(1):54-63. doi:10.5021/ad.2018.30.1.54.
[10]Hoi SY,Lee YJ,Kim JM,et al. Epidermal growth factor relievesinflammatory signals in staphylococcus aureus-treated humanepidermal keratinocytes and atopic dermatitis-like skin lesions inNc/Nga Mice[J]. Biomed Res Int,2018,2018:9439182. doi:10.1155/2018/9439182.
[11]张秀丽,张理涛. rhEGF凝胶对面部激素依赖性皮炎皮肤功能改善的临床研究[J].天津医药,2016,45(5):629-631.ZhangXL,Zhang LT. Clinical study on the efficiency of rhEGF gel to skinfunction of facial hormone-dependent dermatitis[J]. Tianjin Med J,2016,45(5):629-631. doi:10.11958/20150434.
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