骨髓间充质干细胞复合支架材料治疗骨缺损:研究现状及前景展望
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摘要
背景:骨缺损是临床常见且较难解决的问题,骨髓间充质干细胞复合支架材料治疗骨缺损是目前研究的热点。目的:总结骨髓间充质干细胞复合支架材料治疗骨缺损的最新研究进展,为临床应用提供理论依据。方法:文章第一作者通过检索中国知网、万方、维普、PubMed、Embase和WebofScience数据库2009年1月至2018年12月相关文献,检索词为"骨髓间充质干细胞,分离培养,支架材料,骨缺损,骨组织工程,成骨分化,新生血管,骨膜,细胞外基质""bone marrow mesenchymal stem cells,isolation,scaffolds,bone defects,bone tissue engineering,osteogenesis,angiogenesis,periosteum,extracellular matrix"。最终选取符合纳入标准的文献共计95篇。结果与结论:大量研究表明,骨髓间充质干细胞是治疗骨缺损的理想种子细胞,其与支架材料复合能够促进骨缺损修复。目前,骨髓间充质干细胞复合支架材料治疗骨缺损正处于新的发展时代,是一种很有前景的骨缺损治疗方法,其中在骨髓间充质干细胞的分离培养、定向分化及支架材料研究方面取得了很大的进展,并进一步证明了新生血管、骨膜和细胞外基质在整个骨组织工程中的重要作用,这一研究对未来临床治疗骨缺损具有重大意义。
BACKGROUND: Bone defect is a common problem encountered in clinical practice. To this end, bone marrow mesenchymal stem cells combined with scaffolds is a hot topic in the treatment of bone defects.OBJECTIVE: To summarize the latest research progress in bone marrow mesenchymal stem cells combined with scaffolds in the treatment of bone defects, which provides a theoretical basis for clinical application.METHODS: The first author searched CNKI, WanFang, VIP, PubMed, Embase and Web of Science databases from January 2009 to December 2018. Search terms were "bone marrow mesenchymal stem cells, isolation, scaffolds, bone defects, bone tissue engineering,osteogenesis, angiogenesis, periosteum, extracellular matrix" in Chinese and English. Totals of 95 articles that met the inclusion criteria were selected.RESULTS AND CONCLUSION: Numerous studies have shown that bone marrow mesenchymal stem cells are ideal seed cells for the treatment of bone defects, and cell-scaffold composites can promote the repair and healing of bone defects. To date, bone marrow mesenchymal stem cells combined with scaffolds as a promising treatment for bone defects have been in the new development era. Great progress has been achieved in terms of isolation, culture and directed differentiation of bone marrow mesenchymal stem cells as well as scaffold materials. These findings further indicate the important roles of new vessels, periosteum and extracellular matrix in bone tissue engineering, which are of great significance for the future clinical treatment of bone defects
BACKGROUND: Bone defect is a common problem encountered in clinical practice. To this end, bone marrow mesenchymal stem cells combined with scaffolds is a hot topic in the treatment of bone defects.OBJECTIVE: To summarize the latest research progress in bone marrow mesenchymal stem cells combined with scaffolds in the treatment of bone defects, which provides a theoretical basis for clinical application.METHODS: The first author searched CNKI, WanFang, VIP, PubMed, Embase and Web of Science databases from January 2009 to December 2018. Search terms were "bone marrow mesenchymal stem cells, isolation, scaffolds, bone defects, bone tissue engineering,osteogenesis, angiogenesis, periosteum, extracellular matrix" in Chinese and English. Totals of 95 articles that met the inclusion criteria were selected.RESULTS AND CONCLUSION: Numerous studies have shown that bone marrow mesenchymal stem cells are ideal seed cells for the treatment of bone defects, and cell-scaffold composites can promote the repair and healing of bone defects. To date, bone marrow mesenchymal stem cells combined with scaffolds as a promising treatment for bone defects have been in the new development era. Great progress has been achieved in terms of isolation, culture and directed differentiation of bone marrow mesenchymal stem cells as well as scaffold materials. These findings further indicate the important roles of new vessels, periosteum and extracellular matrix in bone tissue engineering, which are of great significance for the future clinical treatment of bone defects
引文
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[19]Nicodemou A,Danisovic L.Mesenchymal stromal/stem cell separation methods:concise review.Cell Tissue Bank.2017;18(4):443-460.
[20]Dvorakova J,Hruba A,Velebny V,et al.Isolation and characterization of mesenchymal stem cell population entrapped in bone marrow collection sets.Cell Biol Int.2008;32(9):1116-1125.
[21]Hung SC,Chen NJ,Hsieh SL,et al.Isolation and characterization of size-sieved stem cells from human bone marrow.Stem Cells.2002;20(3):249-258.
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