基于灰色关联分析探索艾纳香非挥发性部位的抗炎活性谱效关系
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摘要
目的:研究艾纳香非挥发性部位UPLC指纹图谱与抗炎药效之间的相关性,为明确艾纳香抗炎药效物质基础提供实验依据。方法:采用UPLC建立12批艾纳香非挥发性部位的指纹图谱,运用UPLC-Q-TOF-MS对艾纳香非挥发性部位的共有指纹峰进行指认,通过二甲苯致小鼠耳廓肿胀炎症模型获取相应的药效数据,通过灰色关联分析建立其谱效关系。结果:UPLC指纹图谱显示艾纳香非挥发性部位有14个共有峰,与抗炎药效的关联度处于0.717 1~0.550 5,各特征峰所代表的化学成分对其抗炎药效贡献的大小顺序为3号峰> 9号峰> 4号峰> 11号峰> 2号峰> 1号峰> 14号峰> 7号峰> 6号峰> 5号峰> 12号峰> 8号峰> 10号峰> 13号峰,并指认了其中9个共有峰,对抗炎药效贡献较大的前2个共有峰(3号和9号)对应的化学成分分别为3-O-咖啡酰基奎宁酸和3,5-O-二咖啡酰基奎宁酸。结论:通过谱效关系研究获得了艾纳香非挥发性部位的抗炎药效物质群,其抗炎药效是多种成分共同作用的结果,明确咖啡酰基奎宁酸类化合物为艾纳香非挥发性部位发挥抗炎药效的主要物质基础。
Objective:To study the correlation between UPLC fingerprint of anti-inflammatory effect of active components from nonvolatile fraction of Blumea balsamifera,and to provide the basis for clarifying the antiinflammatory material basis of B.balsamifera.Method:UPLC was used to establish fingerprint of nonvolatile fraction of 12 batches of B.balsamifera and their common fingerprint peaks were identified by UPLC-Q-TOF-MS.The corresponding pharmacodynamic data were obtained by auricle swelling and inflammation model mice induced by xylene,and spectrum-effect relationship was established by gray correlation analysis.Result:A total of 14 common peaks in nonvolatile fraction of B.balsamifera were established by UPLC fingerprint and 9 common peaks of them were identified.The correlation between UPLC fingerprint and the anti-inflammatory activity was from0.717 1 to 0.550 5.The contribution of chemical compositions represented by each characteristic peak to the antiinflammatory efficacy was in the order of peak 3 > peak 9 > peak 4 > peak 11 > peak 2 > peak 1 > peak 14 > peak7 > peak 6 > peak 5 > peak 12 > peak 8 > peak 10 > peak 13,and the top two peaks with strong contribution to anti-inflammatory effect were peak 3 and peak 9,they were 3-O-caffeoylquinic acid and 3,5-di-O-caffeoylquinic acid identified by contrast reference substances,respectively.Conclusion:The active substances in nonvolatile fraction of B.balsamifera are obtained through the study on the relationship between spectrum and efficiency,and the anti-inflammatory efficacy of the nonvolatile fraction is the result of combination of various components.It is clear that the caffeoylquinic acid derivates act as predominant anti-inflammatory active substance of nonvolatile fraction of B.balsamifera.
Objective:To study the correlation between UPLC fingerprint of anti-inflammatory effect of active components from nonvolatile fraction of Blumea balsamifera,and to provide the basis for clarifying the antiinflammatory material basis of B.balsamifera.Method:UPLC was used to establish fingerprint of nonvolatile fraction of 12 batches of B.balsamifera and their common fingerprint peaks were identified by UPLC-Q-TOF-MS.The corresponding pharmacodynamic data were obtained by auricle swelling and inflammation model mice induced by xylene,and spectrum-effect relationship was established by gray correlation analysis.Result:A total of 14 common peaks in nonvolatile fraction of B.balsamifera were established by UPLC fingerprint and 9 common peaks of them were identified.The correlation between UPLC fingerprint and the anti-inflammatory activity was from0.717 1 to 0.550 5.The contribution of chemical compositions represented by each characteristic peak to the antiinflammatory efficacy was in the order of peak 3 > peak 9 > peak 4 > peak 11 > peak 2 > peak 1 > peak 14 > peak7 > peak 6 > peak 5 > peak 12 > peak 8 > peak 10 > peak 13,and the top two peaks with strong contribution to anti-inflammatory effect were peak 3 and peak 9,they were 3-O-caffeoylquinic acid and 3,5-di-O-caffeoylquinic acid identified by contrast reference substances,respectively.Conclusion:The active substances in nonvolatile fraction of B.balsamifera are obtained through the study on the relationship between spectrum and efficiency,and the anti-inflammatory efficacy of the nonvolatile fraction is the result of combination of various components.It is clear that the caffeoylquinic acid derivates act as predominant anti-inflammatory active substance of nonvolatile fraction of B.balsamifera.
引文
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[20]Correa V G,Gon9alves G A,de Sá-Nakanishi A B,et al.Effects of in vitro,digestion and in vitro,colonic fermentation on stability and functional properties of yerba mate(Ilex paraguariensis A.St.Hil.)beverages[J].Food Chem,2017,237:453-460.
[21]DING G,WANG Y,LIU A,et al.From chemical markers to quality markers:an integrated approach of UPLC/Q-TOF,NIRS,and chemometrics for the quality assessment of honeysuckle buds[J].Rsc Advances,2017,7(36):22034-22044.
[22]游飞祥,韩彦琪,龚苏晓,等.HPLC-Q-TOF-MS分析植物凉茶中的化学成分[J].食品研究与开发,2016,37(8):161-165.
[23]李泮霖,李楚源,刘孟华,等.基于UFLC-Triple-Q-TOF-MS/MS技术的金银花、山银花化学成分比较[J].中南药学,2016,14(4):363-369.
[24]刘威,王振中,胡军华,等.不同产地牡丹皮中微量元素与多指标成分灰色关联度评价及相关性分析[J].中国实验方剂学杂志,2017,23(1):34-41.
[25]林梦雅,张玉萍,李雅,等.基于灰色关联度分析的丹参提取物抗炎作用谱效关系研究[J].中草药,2017,48(16):3447-3452.
[26]梁健钦,王剑,熊万娜,等.基于灰色关联分析的芒果叶提取物抗炎作用的谱效关系[J].中国实验方剂学杂志,2015,21(1):121-125.
[27]李婷婷,王新芳,马玲,等.谱效关系与一测多评相结合全面提升中药材质量控制标准[J].中国实验方剂学杂志,2014,20(19):225-228.
[28]吕邵娃,董书羽,郭玉岩,等.数据分析技术在中药谱效关系中的应用进展[J].中国实验方剂学杂志,2015,21(15):226-230.
[29]董莉.黑骨藤抗炎作用及质量控制研究[D].兰州:兰州大学,2017.
[30]李祖晟.二咖啡酰奎宁酸药理实验研究进展[J].医学综述,2004,10(4):249-251.
[31]Shin H S,Satsu H,Bae M J,et al.Anti-inflammatory effect of chlorogenic acid on the IL-8 production in Caco-2 cells and the dextran sulphate sodium-induced colitis symptoms in C57BL/6 mice[J].Food Chem,2015,168:167-175.
[32]GUO Y J,LUO T,WU F,et al.Involvement of TLR2 and TLR9 in the anti-inflammatory effects of chlorogenic acid in HSV-1-infected microglia[J].Life Sci,2015,127:12-18.
[2]官玲亮,庞玉新,王丹,等.中国民族特色药材艾纳香研究进展[J].植物遗传资源学报,2012,13(4):695-698.
[3]陈铭.艾纳香的活性成分研究[D].上海:上海交通大学,2009.
[4]吴镝,解朝霞,吴波.银丹心脑通软胶囊治疗心脑血管疾病的疗效分析[J].中国医药指南,2012,10(12):274-275.
[5]王嵩,赵永恒,周毅生,等.艾纳香的研究进展及其研究价值探讨[J].中国现代中药,2014,16(11):953-956.
[6]吴丽芬.艾纳香提取物质量标准与GC指纹图谱的研究[D].广州:广东药学院,2015.
[7]庞玉新,袁蕾,王中洋,等.艾纳香不同部位提取物的抗氧化活性及其对酪氨酸酶的抑制作用[J].中国实验方剂学杂志,2014,20(18):4-8.
[8]杨巧芳,孟庆刚.炎症动物模型探要[J].中华中医药学刊,2008,26(3):516-517.
[9]李波.艾叶油的抗炎活性研究[D].晋中:山西农业大学,2013.
[10]马青松,王丹,庞玉新,等.艾纳香油对小鼠耳肿胀的抗炎效果[J].贵州农业科学,2016,44(4):100-102.
[11]赵桂芝,王绪平,俞忠明,等.艾叶挥发油对耳肿胀急性炎症模型小鼠的抗炎作用研究[J].浙江中医杂志,2016,51(4):288-289.
[12]李小芬,吴红梅,杨烨,等.贵州不同产地艾纳香药材抗炎作用比较[J].时珍国医国药,2018,29(1):200-201.
[13]夏嬿,李祥,陈建伟.艾纳香叶废弃物镇痛、抗炎、止血活性的初步研究[J].天然产物研究与开发,2015,27(6):1086-1091.
[14]ZHU C S,ZHANG B,LIN Z J,et al.Relationship between high-performance liquid chromatography fingerprints and uric acid-lowering activities of Cichorium intybus L.[J].Molecules,2015,20(5):9455-9467.
[15]于海帅.基于主成分分析、聚类分析和典型相关分析的漏芦抗胃癌谱效关系探索[J].中国实验方剂学杂志,2016,22(21):27-31.
[16]刘莉,李文兰,丁晶鑫.基于“谱-效”相关的中药药效物质基础研究方法[J].天然产物研究与开发,2013,25(3):410-415.
[17]谭振鹏,夏英杰,王柳萍,等.中药谱效关系研究进展[J].中国民族民间医药,2013,22(2):20-22.
[18]张颖.中药谱效关系在中药研究中的现状与展望[J].黑龙江医药,2010,23(5):755-758.
[19]杨烨.伸筋草、艾纳香等四味药材化学指纹图谱整体性和模糊性的质量评价研究[D].贵阳:贵阳中医学院,2014.
[20]Correa V G,Gon9alves G A,de Sá-Nakanishi A B,et al.Effects of in vitro,digestion and in vitro,colonic fermentation on stability and functional properties of yerba mate(Ilex paraguariensis A.St.Hil.)beverages[J].Food Chem,2017,237:453-460.
[21]DING G,WANG Y,LIU A,et al.From chemical markers to quality markers:an integrated approach of UPLC/Q-TOF,NIRS,and chemometrics for the quality assessment of honeysuckle buds[J].Rsc Advances,2017,7(36):22034-22044.
[22]游飞祥,韩彦琪,龚苏晓,等.HPLC-Q-TOF-MS分析植物凉茶中的化学成分[J].食品研究与开发,2016,37(8):161-165.
[23]李泮霖,李楚源,刘孟华,等.基于UFLC-Triple-Q-TOF-MS/MS技术的金银花、山银花化学成分比较[J].中南药学,2016,14(4):363-369.
[24]刘威,王振中,胡军华,等.不同产地牡丹皮中微量元素与多指标成分灰色关联度评价及相关性分析[J].中国实验方剂学杂志,2017,23(1):34-41.
[25]林梦雅,张玉萍,李雅,等.基于灰色关联度分析的丹参提取物抗炎作用谱效关系研究[J].中草药,2017,48(16):3447-3452.
[26]梁健钦,王剑,熊万娜,等.基于灰色关联分析的芒果叶提取物抗炎作用的谱效关系[J].中国实验方剂学杂志,2015,21(1):121-125.
[27]李婷婷,王新芳,马玲,等.谱效关系与一测多评相结合全面提升中药材质量控制标准[J].中国实验方剂学杂志,2014,20(19):225-228.
[28]吕邵娃,董书羽,郭玉岩,等.数据分析技术在中药谱效关系中的应用进展[J].中国实验方剂学杂志,2015,21(15):226-230.
[29]董莉.黑骨藤抗炎作用及质量控制研究[D].兰州:兰州大学,2017.
[30]李祖晟.二咖啡酰奎宁酸药理实验研究进展[J].医学综述,2004,10(4):249-251.
[31]Shin H S,Satsu H,Bae M J,et al.Anti-inflammatory effect of chlorogenic acid on the IL-8 production in Caco-2 cells and the dextran sulphate sodium-induced colitis symptoms in C57BL/6 mice[J].Food Chem,2015,168:167-175.
[32]GUO Y J,LUO T,WU F,et al.Involvement of TLR2 and TLR9 in the anti-inflammatory effects of chlorogenic acid in HSV-1-infected microglia[J].Life Sci,2015,127:12-18.
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