基于高通量测序的紫花丹参与白花丹参根际细菌群落结构研究
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摘要
根际细菌在提高植物营养吸收、促进植株生长及提高植物根系抗病性等方面起着重要作用。该研究以同一地块紫花丹参与白花丹参根际细菌为研究对象,采用16S r DNA高通量测序技术研究其群落结构特征与差异性物种,并分析其优势菌群的功能。结果表明,紫花丹参根际的鞘脂杆菌目、鞘脂单胞菌目、亚硝化单胞菌科等优势菌群丰度显著高于白花丹参。丹参根际优势促生细菌的功能主要集中于促进根际土壤养分的转化、降解自毒物质以及提高植物抗环境胁迫能力。该研究首次比较分析了紫花丹参与白花丹参根际细菌群落结构与差异性物种组成,为进一步研究丹参根际微生态过程提供了科学依据。
Rhizosphere bacteria play a vital role in plant nutrition absorption,growth and disease resistance. In this study,high-throughput sequencing technology was used to analyze the rhizosphere bacterial communities of Salvia miltiorrhiza and S. miltiorrhiza f. alba. Moreover,the function of dominant rhizosphere bacterial communities was analyzed. We found that Sphingobacteriales,Sphingomonadales and Nitrosomonadaceae were both dominant and specific bacteria in the rhizosphere of S. miltiorrhiza. The main functions of dominant rhizosphere bacteria communities in both species include promoting transformation of soil nutrients,improving plant immunity and ability of stress tolerance. This study was the first to compare rhizobacterial communities structure and function of S. miltiorrhiza and S. miltiorrhiza f. alba,which provided a new theoretical reference for studing the rhizosphere mechanism of healthy S. miltiorrhiza planting in the future.
Rhizosphere bacteria play a vital role in plant nutrition absorption,growth and disease resistance. In this study,high-throughput sequencing technology was used to analyze the rhizosphere bacterial communities of Salvia miltiorrhiza and S. miltiorrhiza f. alba. Moreover,the function of dominant rhizosphere bacterial communities was analyzed. We found that Sphingobacteriales,Sphingomonadales and Nitrosomonadaceae were both dominant and specific bacteria in the rhizosphere of S. miltiorrhiza. The main functions of dominant rhizosphere bacteria communities in both species include promoting transformation of soil nutrients,improving plant immunity and ability of stress tolerance. This study was the first to compare rhizobacterial communities structure and function of S. miltiorrhiza and S. miltiorrhiza f. alba,which provided a new theoretical reference for studing the rhizosphere mechanism of healthy S. miltiorrhiza planting in the future.
引文
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[31]汪其同,朱婉芮,刘梦玲,等.基于高通量测序的杨树人工林根际和非根际细菌群落结构比较[J].应用与环境生物学报,2015,21(5):967.
[32] Kim Y,Liesack W. Differential assemblage of functional units in paddy soil microbiomes[J]. PLoS ONE, 2015, 10(4):e0122221.
[33]贺纪正,张丽梅.土壤氮素转化的关键微生物过程及机制[J].微生物学通报,2013,40(1):98.
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[35]赵宁.外施矿质元素对月季及月季长管蚜影响的研究[D].昆明:西南林业大学,2011.
[36] Gullner G,Zechmann B,Künstler A,et al. The signaling roles of glutathione in plant disease resistance[M]//Hossain M,Mostofa M,Diaz-Vivancos P,et al. Glutathione in plant growth,development,and stress tolerance. Cham:Springer,2017:331.
[37] Patel M,Kothari I L,Mohan J S. Plant defense induced in in vitro propagated banana(Musa paradisiaca)plantlets by Fusarium derived elicitors[J]. Indian J Exp Biol,2004,42(7):728.
[38]周冀衡,李卫芳.钾对病毒侵染后烟草叶片内源保护酶活性的影响[J].中国农业科学,2000,33(6):98.
[39] Pegoraro C,Mertz L M,Maia L C D,et al. Importance of heat shock proteins in maize[J]. J Crop Sci Biot,2011,14(2):85.
[40] Wang L,Zhao C M,Wang Y J,et al. Overexpression of chloroplast-localized small molecular heat-shock protein enhances chilling tolerance in tomato plant[J]. Acta Photophysiol Sin,2005,31(2):167.
[41] Shinozaki K,Yamaguchi-Shinozaki K. 10-molecular responses to drought stress[M]//Kimiyuki Satoh,Norio Murata. Stress responses of photosynthetic organisms. Amsterdam:Elsevier Science,1998:149.
[42]聂铭.不同连作年限地黄生长生理特性及其根区土壤化感物质研究[D].郑州:河南农业大学,2017.
[43]杨先国,刘塔斯,陈斌,等.丹参根际土壤浸提物的GC-MS分析[J].中国农学通报,2013,29(10):173.
[44] Dennis P G,Miller A J,Hirsch P R. Are root exudates more important than other sources of rhizodeposits in structuring rhizosphere bacterial communities?[J]. FEMS Microbiol Ecol,2010,72(3):313.
[45]邱文龙.不同品种烟草根系分泌物的组分分析与抗黑胫病的关系[D].泰安:山东农业大学,2014.
[2]杨先国,刘塔斯,林丽美,等.湖南地区白花丹参的引种试验研究[J].中国实验方剂学杂志,2012,18(1):117.
[3]李长峰.丹参与白花丹参的比较研究[D].济南:山东中医药大学,2008.
[4]刘伟,张琳,周洁,等.白花丹参与丹参活性成分积累比较研究[J].植物科学学报,2013,31(6):596.
[5]周洁,王晓,刘建华,等.白花丹参和紫花丹参对菘蓝化感效应比较研究[J].中药材,2013,36(9):1389.
[6]周洁,王晓,刘建华,等.丹参和白花丹参对黄芩化感效应的比较研究[J].山东科学,2012,25(5):30.
[7]王铁霖,关巍,孙楷,等.丹参常见病害的病原、发病规律及综合防治[J].中国中药杂志,2018,43(11):2402.
[8] Hiddink G A,Termorshuizen A J,van Bruggen A H C. Mixed cropping and suppression of soilborne diseases[M]//Lichtfouse E. Genetic engineering,biofertilisation,soilquality and organic farming,sustainable agriculture reviews. Netherlands:Springer,2010:119.
[9] Alvey S,Yang C H,Buerkert A,et al. Cereal/legume rotation effects on rhizosphere bacterial community structure in West African soils[J]. Biol Fert Soils,2003,37(2):73.
[10] Corpas F J,Leterrier M,Valderrama R,et al. Nitric oxide imbalance provokes a nitrosative response in plants under abiotic stress[J]. Plant Sci,2011,181(5):604.
[11] Marschner P,Crowley D,Rengel Z. Rhizosphere interactions between microorganisms and plants govern iron and phosphorus acquisition along the root axis-model and research methods[J].Soil Biol Biochem,2011,43(5):883.
[12] Hu X F,Chen J S,Guo J F. Two phosphate-and potassium-solubilizing bacteria isolated from Tianmu Mountain,Zhejiang,China[J]. World J Microb Biot,2006,22(9):983.
[13] Mendes L W,Kuramae E E,Navarrete A A,et al. Taxonomical and functional microbial community selection in soybean rhizosphere[J]. Isme J,2014,8(8):1577.
[14] Kai M,Haustein M,Molina F,et al. Bacterial volatiles and their action potential[J]. Appl Microbiol Biotechnol,2009,81(6):1001.
[15] Wei W,Yu B,Chen Y,et al. Antagonistic activities of volatiles from four strains of Bacillus spp. and Paenibacillus spp. against soil-borne plant pathogens[J]. Agr Sci China,2008,7(9):1104.
[16] Jaleel C A,Manivannan P,Sankar B,et al. Pseudomonasfluorescens enhances biomass yield and ajmalicineproduction in Catharanthus roseus under water deficitstress[J]. Colloid Surface B,2007,60(1):7.
[17] Castiglioni P,Warner D,Bensen R J,et al. Bacterial RNA chaperones confer abiotic stress tolerance in plants and improved grain yield in maize under water-limited conditions[J]. Plant Physiol,2008,147(2):446.
[18] Zhang H,Kim M S,Sun Y,et al. Soil bacteria confer plant salt tolerance by tissue-specific regulation of the sodium transporter HKT1[J]. Mol Plant Microbe In,2008,21(6):737.
[19] Dodd I C,Pérezalfocea F. Microbial amelioration of crop salinity stress[J]. J Exp Bot,2012,63(9):3415.
[20] Marschner P,Yang C H,Lieberei R,et al. Soil and plant specific effects on bacterial community composition in the rhizosphere[J]. Soil Biol Biochem,2001,33(11):1437.
[21] Lundberg D S,Lebeis S L,Paredes S H,et al. Defining the core Arabidopsis thaliana root microbiome[J]. Nature,2012,488(7409):86.
[22] Haney C H,Samuel B S,Bush J,et al. Associations with rhizosphere bacteria can confer an adaptive advantage to plants[J].Nat Plants,2015,1(6):15051.
[23] Marcel M. Cutadapt removes adapter sequences from highthroughput sequencing reads[J]. Embnet J,2011,17(1):10.
[24] Edgar R C,Haas B J,Clemente J C,et al. UCHIME improves sensitivity and speed of chimera detection[J]. Bioinformatics,2011,27(16):2194.
[25] Haas B J,Gevers D,Earl A M,et al. Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons[J]. Genome Res,2011,21(3):494.
[26] Qiong W,Garrity G M,Tiedje J M,et al. Nave bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy[J]. Appl Environ Microb,2007,73(16):5261.
[27] Quast C,Pruesse E,Yilmaz P,et al. The SILVA ribosomal RNA gene database project:improved data processing and web-based tools[J]. Nucl Acids Res,2013,41:D590.
[28]陈文新,汪恩涛.中国根瘤菌[M].北京:科学出版社,2010.
[29] Cernava T,Erlacher A,Aschenbrenner I A,et al. Deciphering functional diversification within the lichen microbiota by metaomics[J]. Microbiome,2017,5(1):82.
[30] Prosser J I,Head I M,Stein L Y. The family Nitrosomonadaceae[M]. Berlin Heidelberg:Springer,2014.
[31]汪其同,朱婉芮,刘梦玲,等.基于高通量测序的杨树人工林根际和非根际细菌群落结构比较[J].应用与环境生物学报,2015,21(5):967.
[32] Kim Y,Liesack W. Differential assemblage of functional units in paddy soil microbiomes[J]. PLoS ONE, 2015, 10(4):e0122221.
[33]贺纪正,张丽梅.土壤氮素转化的关键微生物过程及机制[J].微生物学通报,2013,40(1):98.
[34]李鑫.矿质元素调控烟草抗PVY-N生理生化及分子机制研究[D].沈阳:沈阳农业大学,2009.
[35]赵宁.外施矿质元素对月季及月季长管蚜影响的研究[D].昆明:西南林业大学,2011.
[36] Gullner G,Zechmann B,Künstler A,et al. The signaling roles of glutathione in plant disease resistance[M]//Hossain M,Mostofa M,Diaz-Vivancos P,et al. Glutathione in plant growth,development,and stress tolerance. Cham:Springer,2017:331.
[37] Patel M,Kothari I L,Mohan J S. Plant defense induced in in vitro propagated banana(Musa paradisiaca)plantlets by Fusarium derived elicitors[J]. Indian J Exp Biol,2004,42(7):728.
[38]周冀衡,李卫芳.钾对病毒侵染后烟草叶片内源保护酶活性的影响[J].中国农业科学,2000,33(6):98.
[39] Pegoraro C,Mertz L M,Maia L C D,et al. Importance of heat shock proteins in maize[J]. J Crop Sci Biot,2011,14(2):85.
[40] Wang L,Zhao C M,Wang Y J,et al. Overexpression of chloroplast-localized small molecular heat-shock protein enhances chilling tolerance in tomato plant[J]. Acta Photophysiol Sin,2005,31(2):167.
[41] Shinozaki K,Yamaguchi-Shinozaki K. 10-molecular responses to drought stress[M]//Kimiyuki Satoh,Norio Murata. Stress responses of photosynthetic organisms. Amsterdam:Elsevier Science,1998:149.
[42]聂铭.不同连作年限地黄生长生理特性及其根区土壤化感物质研究[D].郑州:河南农业大学,2017.
[43]杨先国,刘塔斯,陈斌,等.丹参根际土壤浸提物的GC-MS分析[J].中国农学通报,2013,29(10):173.
[44] Dennis P G,Miller A J,Hirsch P R. Are root exudates more important than other sources of rhizodeposits in structuring rhizosphere bacterial communities?[J]. FEMS Microbiol Ecol,2010,72(3):313.
[45]邱文龙.不同品种烟草根系分泌物的组分分析与抗黑胫病的关系[D].泰安:山东农业大学,2014.