26份芸芥种质的耐盐性评价
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摘要
为了明确26份芸芥种质的耐盐性差异,本试验采用6个不同浓度盐溶液,包括0, 0.2%, 0.4%, 0.6%, 0.8%和1.0%NaCl处理芽期芸芥,测定了发芽率、胚根长等指标;筛选出0.6%、0.8%NaCl溶液处理苗期芸芥,测定了幼苗生长及耐盐生理相关指标,并采用模糊数学隶属函数法评价不同芸芥材料的耐盐性。结果表明:(1) 0.6%盐胁迫所造成的芸芥萌发抑制率接近50%,0.8%盐胁迫基本造成了生长的停滞(发芽率8%),因此认为这两个浓度是盐胁迫抑制芸芥生长的阈值。(2)随着盐浓度的增加,正常种苗率变小,其均值变化为57.62%～34.45%;苗高、根长、根鲜重、苗鲜重均下降,其中各指标的变幅分别为0.99～0.77 cm,0.69～0.44 cm,0.0034～0.0023 g,0.0244～0.0176 g。(3)根据隶属函数总平均值大小将参试材料进行耐盐性分类:耐盐性较强的材料(0.6133≤隶属值总平均值≤0.7261),包括08武芸3-1等;耐盐性中等的材料(0.4274≤隶属值总平均值≤0.5911),包括临洮芸芥等;耐盐性较弱的材料(0.3894≤隶属值总平均值≤0.0280),包括13芸芥68-3等。(4)耐盐材料中游离脯氨酸和可溶性糖含量均显著高于耐盐性较差的材料。总之,26份芸芥种质材料对NaCl溶液胁迫的耐受性存在明显差异,通过模糊数学隶属函数法综合苗期各指标筛选出的耐盐材料,可进一步用于耐盐育种与耐盐基因挖掘。
To assess the salt tolerance of 26 Eruca sativa Mill., 6 concentration of NaCl solution were used,including 0, 0.2%, 0.4%, 0.6%, 0.8% and 1.0%, to treat Eruca sativa at germination stage, and the germination rate, radicles length were measured. 0.6% and 0.8% NaCl solution were selected to treat the seedling of Eruca sativa, the physiological indexes of seedling growth and salt tolerance were detemined, and all resources were sorted out using the method of membership function. Results showed that:(1) Tests on seed germination indicated that stress of 0.6% NaCl had an inhabitation of 50% nearly, salt stress of 0.8% NaCl basically caused growth stagnation, therefore, these two concentrations were the thresholds of salt stress inhibiting the growth of Eruca sativa;(2) Survival seedlings, shoot height, root length, fresh weight of shoot and root were all decreased with the increase of salt concentration, the mean were 57.62%～34.45%, 0.99～0.77 cm, 0.69～0.44 cm, 0.0034～0.0023 g, 0.0244～0.0176 g, respectively;(3) Based on the membership function on the means of parameters at seedling stages for each growth level, 26 materials were classified into three types: high salt tolerance, including 08 wuyun 3-1, etc(0.6133≤the verage of membership function values≤0.7261); salt tolerance, including Lintao yunjie, etc(0.4274≤the verage of membership function values≤0.5911); low salt tolerance, including 13 yunjie 76-4,etc(0.0280≤the verage of membership function values≤0.3894);(4) The contents of free proline and soluble sugar in high salt tolerant meaterials were significantly higher than those in low salt tolerant materials. In conclusion, significant difference in salt tolerance of 26 Eruca sativa were revealed, the salt tolerant materials screened by method of membership function of fuzzy mathematics could be further used in salt-tolerant breeding and gene mining.
To assess the salt tolerance of 26 Eruca sativa Mill., 6 concentration of NaCl solution were used,including 0, 0.2%, 0.4%, 0.6%, 0.8% and 1.0%, to treat Eruca sativa at germination stage, and the germination rate, radicles length were measured. 0.6% and 0.8% NaCl solution were selected to treat the seedling of Eruca sativa, the physiological indexes of seedling growth and salt tolerance were detemined, and all resources were sorted out using the method of membership function. Results showed that:(1) Tests on seed germination indicated that stress of 0.6% NaCl had an inhabitation of 50% nearly, salt stress of 0.8% NaCl basically caused growth stagnation, therefore, these two concentrations were the thresholds of salt stress inhibiting the growth of Eruca sativa;(2) Survival seedlings, shoot height, root length, fresh weight of shoot and root were all decreased with the increase of salt concentration, the mean were 57.62%～34.45%, 0.99～0.77 cm, 0.69～0.44 cm, 0.0034～0.0023 g, 0.0244～0.0176 g, respectively;(3) Based on the membership function on the means of parameters at seedling stages for each growth level, 26 materials were classified into three types: high salt tolerance, including 08 wuyun 3-1, etc(0.6133≤the verage of membership function values≤0.7261); salt tolerance, including Lintao yunjie, etc(0.4274≤the verage of membership function values≤0.5911); low salt tolerance, including 13 yunjie 76-4,etc(0.0280≤the verage of membership function values≤0.3894);(4) The contents of free proline and soluble sugar in high salt tolerant meaterials were significantly higher than those in low salt tolerant materials. In conclusion, significant difference in salt tolerance of 26 Eruca sativa were revealed, the salt tolerant materials screened by method of membership function of fuzzy mathematics could be further used in salt-tolerant breeding and gene mining.
引文
[1] Liang C Z,Meng Z H,Meng Z G,et al.GhABF2,a bZIP transcription factor,confers drought and salinity tolerance in cotton (Gossypium hirsutum L.) [J].Scientific Reports,2016,6:35040.
[2] Li Z G,Duan X Q,Xing M,et al.Methylglyoxal as a novel signal molecule induces the salt tolerance of wheat by regulating the glyoxalase system,the antioxidant system,and osmolytes [J].Protoplasma,2017,254(5):1995-2006.
[3] 陈新,张宗文,吴斌.裸燕麦萌发期耐盐性综合评价与耐盐种质筛选[J].中国农业科学,2014,47(10):2038-2046.
[4] 王治江,刘自刚,孙万仓,等.NaCl和Na2SO4胁迫对白菜型冬油菜种子萌发的影响及其耐盐性分析 [J].干旱地区农业研究,2016,34(6):243-252.
[5] 王遵亲.中国盐渍土[M].北京:科学出版社,1993:250-252.?
[6] 张巧凤,陈宗金,吴纪中,等.小麦种质芽期和苗期的耐盐性鉴定评价[J].植物遗传资源学报,2013,14 (4) :620-626.
[7] 李琳,于裕,蒋永超,等.芸豆苗期耐盐碱性鉴定及品种筛选研究[J].植物生理学报,2016,52 (1):62-72.
[8] 刘自刚,王志江,方圆.NaCl胁迫对白菜型冬油菜种子萌发和幼苗生理的影响[J].中国油料作物学报,2017,39(3):351-359.
[9] 田小霞,毛培春,孟林,等.无芒雀麦苗期耐盐指标筛选及耐盐性综合评价[J].干旱区资源与环境,2017,31(10):156-161.
[10] 王靖泽,高树仁,孙丽芳,等.3个玉米自交系对盐胁迫的生理响应及耐盐性评价[J].干旱地区农业研究,2017,35(2):89-95.
[11] 刘敏轩,张宗文,吴斌.黍稷种质资源芽、苗期耐中性混合盐胁迫评价与耐盐生理机制研究[J].中国农业科学,2012,45(18):3733-3743.
[12] 李丰先,周宇飞,王艺陶,等.高粱品种萌发期耐碱性筛选与综合鉴定[J].中国农业科学,2013,46(9):1762-1771.
[13] Hilda A,Babak D H,Azra A A.Morpho-physiological responses of Rocket (Eruca sativa L.) varieties to sodium sulfate (Na2SO4) stress:an experimental approach [J].Acta Physiol Plant,2016,38(10):246.
[14] 方彦,孙万仓,武军艳.芸芥自交亲和相关基因的差异显示及表达分析[J].中国油料作物学报,2014,36(5):580-585.
[15] 孙万仓.中国芸芥的分布、类型划分及油菜-芸芥杂交亲和性研究[D].长沙:湖南农业大学,2000.
[16] 闫旭东.植物耐盐性鉴定及评价技术规程[M].北京:中国农业科学技术出版社,2012:27-28.
[17] 高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006.
[18] 苏国兴,洪法水.桑品种耐盐性的隶属函数法之评价[J].江苏农业科学,2002,18(1):42-47:144,228-230.
[19] 柴媛媛,史团省,谷卫彬.种子萌发期甜高粱对盐胁迫的响应及其耐盐性综合评价分析[J].种子,2008,27(2):43-46.
[20] 吴家富,杨博文,向珣朝,等.不同水稻种质在不同生育期耐盐鉴定的差异[J].植物学报,2017,52(1):77-88.
[21] 李娜,陈红,裴孝伯,等.外源亚精胺对盐胁迫下黄瓜幼苗耐盐性的影响[J].热带作物学报,2013,34(7):1359-1364.
[22] 于洁,贾振宇,黄帆,等.5份不同来源扁蓿豆幼苗期的耐盐性比较[J].草地学报,2016,24(2):459-461.
[2] Li Z G,Duan X Q,Xing M,et al.Methylglyoxal as a novel signal molecule induces the salt tolerance of wheat by regulating the glyoxalase system,the antioxidant system,and osmolytes [J].Protoplasma,2017,254(5):1995-2006.
[3] 陈新,张宗文,吴斌.裸燕麦萌发期耐盐性综合评价与耐盐种质筛选[J].中国农业科学,2014,47(10):2038-2046.
[4] 王治江,刘自刚,孙万仓,等.NaCl和Na2SO4胁迫对白菜型冬油菜种子萌发的影响及其耐盐性分析 [J].干旱地区农业研究,2016,34(6):243-252.
[5] 王遵亲.中国盐渍土[M].北京:科学出版社,1993:250-252.?
[6] 张巧凤,陈宗金,吴纪中,等.小麦种质芽期和苗期的耐盐性鉴定评价[J].植物遗传资源学报,2013,14 (4) :620-626.
[7] 李琳,于裕,蒋永超,等.芸豆苗期耐盐碱性鉴定及品种筛选研究[J].植物生理学报,2016,52 (1):62-72.
[8] 刘自刚,王志江,方圆.NaCl胁迫对白菜型冬油菜种子萌发和幼苗生理的影响[J].中国油料作物学报,2017,39(3):351-359.
[9] 田小霞,毛培春,孟林,等.无芒雀麦苗期耐盐指标筛选及耐盐性综合评价[J].干旱区资源与环境,2017,31(10):156-161.
[10] 王靖泽,高树仁,孙丽芳,等.3个玉米自交系对盐胁迫的生理响应及耐盐性评价[J].干旱地区农业研究,2017,35(2):89-95.
[11] 刘敏轩,张宗文,吴斌.黍稷种质资源芽、苗期耐中性混合盐胁迫评价与耐盐生理机制研究[J].中国农业科学,2012,45(18):3733-3743.
[12] 李丰先,周宇飞,王艺陶,等.高粱品种萌发期耐碱性筛选与综合鉴定[J].中国农业科学,2013,46(9):1762-1771.
[13] Hilda A,Babak D H,Azra A A.Morpho-physiological responses of Rocket (Eruca sativa L.) varieties to sodium sulfate (Na2SO4) stress:an experimental approach [J].Acta Physiol Plant,2016,38(10):246.
[14] 方彦,孙万仓,武军艳.芸芥自交亲和相关基因的差异显示及表达分析[J].中国油料作物学报,2014,36(5):580-585.
[15] 孙万仓.中国芸芥的分布、类型划分及油菜-芸芥杂交亲和性研究[D].长沙:湖南农业大学,2000.
[16] 闫旭东.植物耐盐性鉴定及评价技术规程[M].北京:中国农业科学技术出版社,2012:27-28.
[17] 高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006.
[18] 苏国兴,洪法水.桑品种耐盐性的隶属函数法之评价[J].江苏农业科学,2002,18(1):42-47:144,228-230.
[19] 柴媛媛,史团省,谷卫彬.种子萌发期甜高粱对盐胁迫的响应及其耐盐性综合评价分析[J].种子,2008,27(2):43-46.
[20] 吴家富,杨博文,向珣朝,等.不同水稻种质在不同生育期耐盐鉴定的差异[J].植物学报,2017,52(1):77-88.
[21] 李娜,陈红,裴孝伯,等.外源亚精胺对盐胁迫下黄瓜幼苗耐盐性的影响[J].热带作物学报,2013,34(7):1359-1364.
[22] 于洁,贾振宇,黄帆,等.5份不同来源扁蓿豆幼苗期的耐盐性比较[J].草地学报,2016,24(2):459-461.