H_2O_2氧化CQDs抑制动力学荧光法测定双酚A
|
摘要
以柠檬皮为原料,采用水热法制备碳量子点,基于类Fenton试剂(Fe~(3+)+H_2O_2)能氧化碳量子点使其荧光猝灭而双酚A对此反应有明显抑制效果的实验现象,发展了一种抑制动力学荧光法用以测定双酚A,并探讨了反应机理.实验结果表明,在选定的实验条件下,当双酚A质量浓度在4.0~60.0mg/L范围内时,荧光强度差与质量浓度呈良好的线性关系,方法的检出限(3σ/k)为2.04mg/L;采用此方法对超市购物小票浸取液及自来水等实际样品中BPA的含量进行测定,加标回收率在93%~108%范围内.
Carbon quantum dots(CQDs)were prepared by hydrothermal treatment with Lemon peel as raw material.Peroxyl radical produced by Fenton-like reagent(Fe~(3+)+H_2O_2)oxidizes CQDs and produces the quenching of its fluorescence.It is found that bisphenol A(BPA)has an inhibitory effect on the redox reaction.Based on this observation,an inhibitory kinetic fluorimetric method is reported for the determination of BPA.Under the optimum experimental conditions,the linear range of the determination is 4.0~60.0 mg/L,and the detection limit(3σ/k)for BPA is 2.04 mg/L.The proposed method has been used for the determination of BPA in the shopping receipt and tap waters samples with recoveries of 93%~108%.
Carbon quantum dots(CQDs)were prepared by hydrothermal treatment with Lemon peel as raw material.Peroxyl radical produced by Fenton-like reagent(Fe~(3+)+H_2O_2)oxidizes CQDs and produces the quenching of its fluorescence.It is found that bisphenol A(BPA)has an inhibitory effect on the redox reaction.Based on this observation,an inhibitory kinetic fluorimetric method is reported for the determination of BPA.Under the optimum experimental conditions,the linear range of the determination is 4.0~60.0 mg/L,and the detection limit(3σ/k)for BPA is 2.04 mg/L.The proposed method has been used for the determination of BPA in the shopping receipt and tap waters samples with recoveries of 93%~108%.
引文
[1]方燕,马琳琳,陕多亮,等.石墨烯-金纳米粒子复合膜修饰电极的制备及对双酚A的测定[J].高等学校化学学报,2015,36(8):1 491-1 497.
[2]张圣虎,张易曦,吉贵祥,等.高效液相色谱-串联质谱法测定儿童和成人尿液中双酚A、四溴双酚A和辛基酚[J].分析化学,2016,44(1):19-24.
[3]杨猛,马红燕.纳米银增强化学发光法测定塑料中的双酚A[J].化学研究与应用,2012,24(5):801-805.
[4]FAN J,GUO H,LIU G,et al.Simple and Sensitive Fluorimetric Method for Determination of Environmental Hormone Bisphenol A Based on Its Inhibitory Effect on the Redox Reaction Between Hydroxy Radical and Rhodamine 6G[J].Anal Chim Acta,2007,585(1):134-138.
[5]苏安梅,钟青梅,余姝轶,等.碳量子点荧光猝灭法测定饮料中日落黄[J].发光学报,2017,38(4):530-534.
[6]HUANG H,WANG B,CHEN M,et al.Fluorescence Turn-on Sensing of Ascorbic Acid and Alkaline Phosphatase Activity Based on Graphene Quantum Dots[J].Sensors and Actuators B:Chemical,2016,235:356-361.
[7]WANG G L,FANG X,WU X M,et al.Label-Free and Ratiometric Detection of Nuclei Acids Based on Graphene Quantum Dots Utilizing Cascade Amplification by Nicking Endonuclease and Catalytic G-Quadruplex DNAzyme[J].Biosens Bioelectron,2016,81:214-220.
[8]马红燕,王艳妮.石墨烯量子点荧光探针测定肾上腺色腙[J].发光学报,2016,37(2):231-236.
[9]PAN J H,ZHENG Z Y,YANG J Y,et al.A Nnovel and Sensitive Fluorescence Sensor for Glutathione Detection by Controlling the Surface Passivation Degree of Carbon Quantum Dots[J].Talanta,2017,166:1-7.
[10]KE J,LI X Y,ZHAO Q D,et al.Upconversion Carbon Quantum Dots as Visible Light Responsive Component for Efficient Enhancement of Photocatalytic Performance[J].J Colloid Interface Sci,2017,496:425-433.
[11]LI H,HE X,LIU Y,et al.One-Step Ultrasonic Synthesis of Water-Soluble Carbon Nanoparticles with Excellent Photoluminescent Properties[J].Carbon,2011,49:605-609.
[12]YUAN M,ZHONG R B,GAO H Y,et al.One-Step,Green,and Economic Synthesis of Water-Soluble Photoluminescent Carbon Dots by Hydrothermal Treatment of Wheat Straw,and Their Bio-Applications in Labeling,Imaging,and Sensing[J].Applied Surface Science,2015,355:1 136-1 144.
[13]CHENG C G,SHI Y N,LI M,et al.Carbon Quantum Dots from Carbonized Walnut Shells:Structural Evolution,Fluorescence Characteristics,and Intracellular Bioimaging[J].Mater Sci Eng C Mater Biol Appl,2017,79:473-480.
[14]KUMARI A,KUMAR A,SAHU S K,et al.Synthesis of Green Fluorescent Carbon Quantum Dots Using Waste Polyolefins Residue for Cu2+Ion Sensing and Live Cell Imaging[J].Sensors and Actuators B:Chemical,2018,254:197-205.
[15]HUA J H,YANG J,ZHU Y,et al.Highly Fluorescent Carbon Quantum Dots as Nanoprobes for Sensitive and Selective Determination of Mercury(II)in Surface Waters[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2017,187:149-155.
[16]WU X L,SONG Y,YAN X,et al.Carbon Quantum Dots as Fluorescence Resonance Energy Transfer Sensorsfor Organophosphate Pesticides Determination[J].Biosens Bioelectron,2017,94:292-297.
[17]MADRAKIAN T,MALEKI S,GILAK S,et al.Turn-Off Fluorescence of Amino-Functionalized Carbon Quantum Dots as Effective Fluorescent Probes for Determination of Isotretinoin[J].Sensors and Actuators B:Chemical,2017,247:428-435.
[18]LI S,ZHANG G S,WANG P,et al.Microwave-Enhanced Mn-Fenton Process for the Removal of BPA in Water[J].Chemical Engineering Journal,2016,294:371-379.
[2]张圣虎,张易曦,吉贵祥,等.高效液相色谱-串联质谱法测定儿童和成人尿液中双酚A、四溴双酚A和辛基酚[J].分析化学,2016,44(1):19-24.
[3]杨猛,马红燕.纳米银增强化学发光法测定塑料中的双酚A[J].化学研究与应用,2012,24(5):801-805.
[4]FAN J,GUO H,LIU G,et al.Simple and Sensitive Fluorimetric Method for Determination of Environmental Hormone Bisphenol A Based on Its Inhibitory Effect on the Redox Reaction Between Hydroxy Radical and Rhodamine 6G[J].Anal Chim Acta,2007,585(1):134-138.
[5]苏安梅,钟青梅,余姝轶,等.碳量子点荧光猝灭法测定饮料中日落黄[J].发光学报,2017,38(4):530-534.
[6]HUANG H,WANG B,CHEN M,et al.Fluorescence Turn-on Sensing of Ascorbic Acid and Alkaline Phosphatase Activity Based on Graphene Quantum Dots[J].Sensors and Actuators B:Chemical,2016,235:356-361.
[7]WANG G L,FANG X,WU X M,et al.Label-Free and Ratiometric Detection of Nuclei Acids Based on Graphene Quantum Dots Utilizing Cascade Amplification by Nicking Endonuclease and Catalytic G-Quadruplex DNAzyme[J].Biosens Bioelectron,2016,81:214-220.
[8]马红燕,王艳妮.石墨烯量子点荧光探针测定肾上腺色腙[J].发光学报,2016,37(2):231-236.
[9]PAN J H,ZHENG Z Y,YANG J Y,et al.A Nnovel and Sensitive Fluorescence Sensor for Glutathione Detection by Controlling the Surface Passivation Degree of Carbon Quantum Dots[J].Talanta,2017,166:1-7.
[10]KE J,LI X Y,ZHAO Q D,et al.Upconversion Carbon Quantum Dots as Visible Light Responsive Component for Efficient Enhancement of Photocatalytic Performance[J].J Colloid Interface Sci,2017,496:425-433.
[11]LI H,HE X,LIU Y,et al.One-Step Ultrasonic Synthesis of Water-Soluble Carbon Nanoparticles with Excellent Photoluminescent Properties[J].Carbon,2011,49:605-609.
[12]YUAN M,ZHONG R B,GAO H Y,et al.One-Step,Green,and Economic Synthesis of Water-Soluble Photoluminescent Carbon Dots by Hydrothermal Treatment of Wheat Straw,and Their Bio-Applications in Labeling,Imaging,and Sensing[J].Applied Surface Science,2015,355:1 136-1 144.
[13]CHENG C G,SHI Y N,LI M,et al.Carbon Quantum Dots from Carbonized Walnut Shells:Structural Evolution,Fluorescence Characteristics,and Intracellular Bioimaging[J].Mater Sci Eng C Mater Biol Appl,2017,79:473-480.
[14]KUMARI A,KUMAR A,SAHU S K,et al.Synthesis of Green Fluorescent Carbon Quantum Dots Using Waste Polyolefins Residue for Cu2+Ion Sensing and Live Cell Imaging[J].Sensors and Actuators B:Chemical,2018,254:197-205.
[15]HUA J H,YANG J,ZHU Y,et al.Highly Fluorescent Carbon Quantum Dots as Nanoprobes for Sensitive and Selective Determination of Mercury(II)in Surface Waters[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2017,187:149-155.
[16]WU X L,SONG Y,YAN X,et al.Carbon Quantum Dots as Fluorescence Resonance Energy Transfer Sensorsfor Organophosphate Pesticides Determination[J].Biosens Bioelectron,2017,94:292-297.
[17]MADRAKIAN T,MALEKI S,GILAK S,et al.Turn-Off Fluorescence of Amino-Functionalized Carbon Quantum Dots as Effective Fluorescent Probes for Determination of Isotretinoin[J].Sensors and Actuators B:Chemical,2017,247:428-435.
[18]LI S,ZHANG G S,WANG P,et al.Microwave-Enhanced Mn-Fenton Process for the Removal of BPA in Water[J].Chemical Engineering Journal,2016,294:371-379.