Fe~(3+)催化二苯胺磺酸钠显色-分光光度法测定消毒液中过氧化氢
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摘要
将1.00mL消毒液样品用蒸馏水稀释至100mL,取5.00mL,与5.0mL的8.0g·L~(-1)二苯胺磺酸钠溶液混合,加入3mol·L~(-1) H_2SO_4溶液2mL,0.1mol·L~(-1) FeCl_3溶液0.5mL,用蒸馏水定容至50.0mL,室温下反应60min,以试剂空白作参比,用1cm的比色皿于波长410nm处测量吸光度。结果表明:过氧化氢浓度在0.116～1.16mmol·L~(-1)内与吸光度呈线性关系,检出限(3s/k)为0.02mmol·L~(-1),表观摩尔吸光率为1.34×10~3L·mol~(-1)·cm~(-1)。方法用于测定消毒液中过氧化氢的含量,测定值与标示值相符,和碘量法的测定结果一致,测定值的相对标准偏差(n=6)小于2.0%。
The disinfectant sample(1.00 mL)was diluted to 100 mL with distilled water.5.00 mL of the solution was mixed with 5.0 mL of 8.0 g·L~(-1) sodium diphenylaminesulfonate solution,and 2 mL of 3 mol·L~(-1) H_2 SO_4 solution,0.5 mL of 0.1 mol·L~(-1) FeCl_3 solution were added.The volume of the solution was made up to50.0 mL with distilled water.After reaction at room temperature for 60 min,the absorbance was measured at the wavelength of 410 nm using a 1 cm cuvette with the reagent blank as a reference.The results showed that linear relationship was found between the absorbance of the solution and the concentration of hydrogen peroxide in the range of 0.116-1.16 mmol·L~(-1).The detection limit(3 s/k)was 0.02 mmol·L~(-1) and the apparent molar absorptivity was 1.34×10~3 L·mol~(-1)·cm~(-1).The proposed method was applied to determination of content of hydrogen peroxide in disinfectant,giving the measured value consistent with the labeled value and the determination result obtained by the iodometric method.The relative standard deviations(n=6)of the measured values were less than 2.0%.
The disinfectant sample(1.00 mL)was diluted to 100 mL with distilled water.5.00 mL of the solution was mixed with 5.0 mL of 8.0 g·L~(-1) sodium diphenylaminesulfonate solution,and 2 mL of 3 mol·L~(-1) H_2 SO_4 solution,0.5 mL of 0.1 mol·L~(-1) FeCl_3 solution were added.The volume of the solution was made up to50.0 mL with distilled water.After reaction at room temperature for 60 min,the absorbance was measured at the wavelength of 410 nm using a 1 cm cuvette with the reagent blank as a reference.The results showed that linear relationship was found between the absorbance of the solution and the concentration of hydrogen peroxide in the range of 0.116-1.16 mmol·L~(-1).The detection limit(3 s/k)was 0.02 mmol·L~(-1) and the apparent molar absorptivity was 1.34×10~3 L·mol~(-1)·cm~(-1).The proposed method was applied to determination of content of hydrogen peroxide in disinfectant,giving the measured value consistent with the labeled value and the determination result obtained by the iodometric method.The relative standard deviations(n=6)of the measured values were less than 2.0%.
引文
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[11]唐宁莉,单雅琦,陈永宁,等.共振散射光谱法测定水中痕量的过氧化氢[J].理化检验-化学分册,2016,52(5):589-592.
[12]颜双,李晓燕,刘志昌,等.2-氨基咪唑血红素模拟酶催化荧光光谱法测定过氧化氢[J].理化检验-化学分册,2015,51(3):300-303.
[13]方艳芬,黄应平,罗光富,等.荧光光度法测定雨水中的H2O2含量[J].光谱学与光谱分析,2008,28(4):917-921.
[14]马美萍,连国军,曹建明.辣根过氧化物酶/多壁碳纳米管修饰铂电极生物传感器用于过氧化氢的循环伏安测定[J].理化检验-化学分册,2011,47(3):279-283.
[15]刘艳,牛卫芬,徐岚.基于层层自组装技术制备石墨烯/多壁碳纳米管共修饰的过氧化氢传感器的研究[J].分析化学,2011,39(11):1676-1681.
[16]张国芳,陈洪渊.流动注射胶束电化学发光测定过氧化氢的研究[J].分析科学学报,2001,17(1):1-5.
[17]陈易晖,刘艳,周建立,等.高效液相色谱-紫外检测法测定食品中的过氧化氢[J].光谱实验室,2009,26(2):414-417.
[18]王尊本,郑朱梓,金敏.一阶导数催化动力学分光光度法测定饮用水中的铁[J].分析试验室,1994,13(5):14-16.
[19]李多孚,谭树民,吴大佩,等.Fe(Ⅲ)-二苯胺磺酸钠-过氧化氢体系测微量铁[J].理化检验-化学分册,1999,35(12):548-549.
[20]陈晓红,徐玲.二苯胺磺酸钠光度法测定Fe(Ⅲ)[J].武汉工程大学学报,2011,33(7):34-35.
[2]陶锁军,张爱菊,张小林.Fe(Ⅱ)-铁氰化钾阻抑分光光度法测定消毒液中过氧化氢[J].理化检验-化学分册,2017,53(2):233-235.
[3]向晓明,李花,张小林.协同增敏Fe(Ⅱ)-邻二氮杂菲阻抑光度法测定微量过氧化氢[J].分析试验室,2015,34(11):1322-1324.
[4] LUO W,ABBAS M E,ZHU L H,et al.Rapid quantitative determination of hydrogen peroxide by oxidation decolorization of methyl orange using a Fenton reaction system[J].Analytica Chimica Acta,2008,629(1/2):1-5.
[5]陈亚红,刘红梅,田丰收,等.酶催化分光光度法测定过氧化氢[J].理化检验-化学分册,2009,45(4):401-403.
[6]唐宁莉,蒙华毅,李欣,等.牛血红蛋白催化分光光度法测定过氧化氢[J].理化检验-化学分册,2014,50(2):191-194.
[7]蔡甫格,王立丰,史敏晶,等.一种测定巴西橡胶树胶乳中过氧化氢含量及其在胶乳各组分中分布的方法研究[J].热带作物学报,2011,32(5):891-894.
[8]杨署光,陈月异,李言.一种测定巴西橡胶树树皮中过氧化氢含量的方法[J].热带作物学报,2018,39(12):2383-2390.
[9]汤亚芳,蒋治良,梁爱惠,等.铜催化过氧化氢氧化纳米银-原子吸收光谱法测定过氧化氢[J].冶金分析,2009,29(3):37-40.
[10]李振中,蒋治良,杨光,等.罗丹明6G缔合微粒共振散射光谱法测定过氧化氢[J].光谱学与光谱分析,2005,25(8):1286-1288.
[11]唐宁莉,单雅琦,陈永宁,等.共振散射光谱法测定水中痕量的过氧化氢[J].理化检验-化学分册,2016,52(5):589-592.
[12]颜双,李晓燕,刘志昌,等.2-氨基咪唑血红素模拟酶催化荧光光谱法测定过氧化氢[J].理化检验-化学分册,2015,51(3):300-303.
[13]方艳芬,黄应平,罗光富,等.荧光光度法测定雨水中的H2O2含量[J].光谱学与光谱分析,2008,28(4):917-921.
[14]马美萍,连国军,曹建明.辣根过氧化物酶/多壁碳纳米管修饰铂电极生物传感器用于过氧化氢的循环伏安测定[J].理化检验-化学分册,2011,47(3):279-283.
[15]刘艳,牛卫芬,徐岚.基于层层自组装技术制备石墨烯/多壁碳纳米管共修饰的过氧化氢传感器的研究[J].分析化学,2011,39(11):1676-1681.
[16]张国芳,陈洪渊.流动注射胶束电化学发光测定过氧化氢的研究[J].分析科学学报,2001,17(1):1-5.
[17]陈易晖,刘艳,周建立,等.高效液相色谱-紫外检测法测定食品中的过氧化氢[J].光谱实验室,2009,26(2):414-417.
[18]王尊本,郑朱梓,金敏.一阶导数催化动力学分光光度法测定饮用水中的铁[J].分析试验室,1994,13(5):14-16.
[19]李多孚,谭树民,吴大佩,等.Fe(Ⅲ)-二苯胺磺酸钠-过氧化氢体系测微量铁[J].理化检验-化学分册,1999,35(12):548-549.
[20]陈晓红,徐玲.二苯胺磺酸钠光度法测定Fe(Ⅲ)[J].武汉工程大学学报,2011,33(7):34-35.