灰霉菌侵染对蓝莓采后品质变化及抗氧化性的影响
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摘要
灰霉病是蓝莓采后高发病害之一,严重影响贮藏品质。以"灿烂"蓝莓为材料,以无菌水处理的果实为对照,研究灰葡萄孢霉侵染对蓝莓采后品质和抗氧化性的影响。结果表明:受侵染的蓝莓果实在贮藏前期活性氧自由基(ROS)迅速积累,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)活性提高,同时抗坏血酸、谷胱甘肽等抗氧化物质含量增加;贮藏后期抗氧化酶活和抗氧化物质含量下降,活性氧清除能力不足导致ROS过度积累,细胞膜结构破坏,加速了蓝莓的成熟、软化、衰老和腐烂。
Gray mold is one of the frequently-occurring postharvest diseases of blueberry, which seriously affects the storage quality. The effects of Botrytis cinerea on the postharvest quality and antioxidant system of 'Brilliant' blueberry were studied with the fruits treated with sterile water as the control. The results showed that the reactive oxygen species(ROS) accumulated rapidly, the activities of superoxide dismutase(SOD), catalase(CAT), ascorbate peroxidase(APX),glycopeptide reductase(GR) and the the contents of ascorbic acid and glutathione increased rapidly in the infected blueberry fruits. At the late storage stage, the activities of antioxidant enzymes and antioxidant level were decreased, and the insufficient scavenging capacity of ROS lead to excessive accumulation of ROS and destruction of cell membrane structure, which accelerates the ripening, softening, aging and decay of blueberries.
Gray mold is one of the frequently-occurring postharvest diseases of blueberry, which seriously affects the storage quality. The effects of Botrytis cinerea on the postharvest quality and antioxidant system of 'Brilliant' blueberry were studied with the fruits treated with sterile water as the control. The results showed that the reactive oxygen species(ROS) accumulated rapidly, the activities of superoxide dismutase(SOD), catalase(CAT), ascorbate peroxidase(APX),glycopeptide reductase(GR) and the the contents of ascorbic acid and glutathione increased rapidly in the infected blueberry fruits. At the late storage stage, the activities of antioxidant enzymes and antioxidant level were decreased, and the insufficient scavenging capacity of ROS lead to excessive accumulation of ROS and destruction of cell membrane structure, which accelerates the ripening, softening, aging and decay of blueberries.
引文
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[14] KRANNER I, GRILL D. Determination of glutathione and glutathione disulphide in lichens:a comparison of frequently used methods[J]. Phytochemical Analysis, 2015, 7(1):24-28.
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[16] WANY Y S, TIAN S P, XU Y. Effects of high oxygen concentration on pro-and anti-oxidant enzymes in peach fruits during postharvest periods[J].Food Chemistry, 2005, 91(1):99-104.
[17] ZHANG Q, LIU Y L, HE C C, et al. Postharvest exogenous application of abscisic acid reduces internal browning in pineapple[J]. Journal of Agricultural&Food Chemistry, 2015, 63(22):5313.
[18] CHU W J, GAO H Y, CHEN H J, et al. Effects of cuticular wax on the postharvest quality of blueberry fruit[J]. Food Chemistry, 2017, 239:68-74.
[19]曹建康,姜微波,赵玉梅.果蔬采后生理生化实验指导[M].北京:中国轻工业出版社, 2007:126-127.
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[22] CHEN Z, SILVA H, KLESSIG D F. Active oxygen species in the induction of plant systemic acquired resistance by salicylic acid[J]. Science(New York,N.Y.), 1993, 262(5141):1883.
[23] BRADLEY D J, KJELLBOM P, LAMB C J. Elicitor-and wound-induced oxidative cross-linking of a proline-rich plant cell wall protein:a novel, rapid defense response[J]. Cell, 1992, 70(1):21-30.
[24] ARUOMA O I, GROOTVELD M, BAHORUN T.Free radicals in biology and medicine:From inflammation to biotechnology[J]. Biofactors, 2010, 27(1/2/3/4):1-3.
[25] LIU J Z, HE C C SHEN F, et al. The crown plays an important role in maintaining quality of harvested pineapple[J]. Postharvest Biology&Technology, 2017, 124:18-24.
[26]陈艺晖,林河通,林艺芬,等.拟茎点霉侵染对龙眼果实采后果皮褐变和活性氧代谢的影响[J].中国农业科学, 2011, 44(23):4858-4866.
[27] SHARMA P, JHA A B, DUBEY R S, et al. Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions[J]. Journal of Botany, 2012,2012:1-26.
[28]杜秀敏,殷文璇,赵彦修,等.植物中活性氧的产生及清除机制[J].生物工程学报, 2001, 17(2):121-125.
[29] AGUDELO-ROMERO P, ERBAN A, REGO C, et al. Transcriptome and metabolome reprogramming in Vitis vinifera cv. Trincadeira berries upon infection with Botrytis cinerea[J]. Journal of Experimental Botany, 2015, 66(7):1769-1785.
[30]李晓云,王秀峰,吕乐福,等.外源NO对铜胁迫下番茄幼苗根系抗坏血酸-谷胱甘肽循环的影响[J].应用生态学报, 2013, 24(4):1023-1030.
[31] MANDAL S, MITRA A, MALLICK N. Biochemical characterization of oxidative burst during interaction between Solanum lycopersicum and Fusarium oxysporum f. sp. lycopersici[J]. Physiological&Molecular Plant Pathology, 2008, 72(1):56-61.
[32]刘晋,刘爱媛,陈维信.霜疫病菌侵染对荔枝果实生理变化的影响[J].西南园艺, 2006, 34(1):1-4.
[2] TOURNAS V H, KATSOUDAS E. Mould and yeast flora in fresh berries, grapes and citrus fruits[J]. International Journal of Food Microbiology, 2005, 105(1):11.
[3] YANY G Y, YUE J, GONG X C, et al. Blueberry leaf extracts incorporated chitosan coatings for preserving postharvest quality of fresh blueberries[J].Postharvest Biology&Technology, 2014, 92(2):46-53.
[4] KWON J H, CHEON M G, CHOI O, et al. First report of Botrytis cinerea as a postharvest pathogen of blueberry in Korea[J]. Mycobiology, 2011, 39(1):52-53.
[5]郜海燕,肖尚月,陈杭君,等.蓝莓采后主要病原真菌的分离鉴定与生物学特性研究[J].农业机械学报, 2017, 48(5):327-334.
[6] HUANG X J, LI J, SHANG H L, et al. Effect of methyl jasmonate on the anthocyanin content and antioxidant activity of blueberries during cold storage[J]. Journal of the Science of Food and Agriculture,2015, 95(2):337-343.
[7]周雁,陈群超,蒋珩珺,等.银/壳聚糖复合物制备及其对蓝莓真菌的抑制作用[J].中国食品学报,2016, 16(3):58-67.
[8]雷纪锋,陈树兵,吴祖芳,等.葡萄中赭曲霉毒素产生真菌的筛选及侵染特性[J].中国食品学报,2017, 17(5):186-193.
[9] CHU W J, GAO H Y, CHEN H J, et al. Effects of cuticular wax on the postharvest quality of blueberry fruit[J]. Food Chemistry, 2017, 239:68-74.
[10]陈杭君,王翠红,郜海燕,等.不同包装方法对蓝莓采后贮藏品质和抗氧化活性的影响[J].中国农业科学, 2013, 46(6):1230-1236.
[11] ARAKAWA N, TSUTSUMI K, SANCEDA N G, et al. A rapid and sensitive method for the determination of ascorbic acid using 4,7-diphenyl-1,10-phenanthroline[J]. Journal of the Agricultural Chemical Society of Japan, 2006, 45(5):1289-1290.
[12] LI X A, LONG Q H, GAO F, et al. Effect of cutting styles on quality and antioxidant activity in fresh-cut pitaya fruit[J]. Postharvest Biology&Technology, 2017, 124:1-7.
[13] FERGUSON I B, WATKINS C B, HARMAN J E.Inhibition by calcium of senescence of detached cucumber cotyledons:Effect on ethylene and hydroperoxide production[J]. Plant Physiology, 1983, 71(1):182-186.
[14] KRANNER I, GRILL D. Determination of glutathione and glutathione disulphide in lichens:a comparison of frequently used methods[J]. Phytochemical Analysis, 2015, 7(1):24-28.
[15] CHENG H J, GAO H Y, FANG X J, et al. Effects of allyl isothiocyanate treatment on postharvest quality and the activities of antioxidant enzymes of mulberry fruit[J]. Postharvest Biology&Technology,2015, 108:61-67.
[16] WANY Y S, TIAN S P, XU Y. Effects of high oxygen concentration on pro-and anti-oxidant enzymes in peach fruits during postharvest periods[J].Food Chemistry, 2005, 91(1):99-104.
[17] ZHANG Q, LIU Y L, HE C C, et al. Postharvest exogenous application of abscisic acid reduces internal browning in pineapple[J]. Journal of Agricultural&Food Chemistry, 2015, 63(22):5313.
[18] CHU W J, GAO H Y, CHEN H J, et al. Effects of cuticular wax on the postharvest quality of blueberry fruit[J]. Food Chemistry, 2017, 239:68-74.
[19]曹建康,姜微波,赵玉梅.果蔬采后生理生化实验指导[M].北京:中国轻工业出版社, 2007:126-127.
[20] APEL K, HIRT H. Reactive oxygen species:metabolism, oxidative stress, and signal transduction[J]. Annual Review of Plant Biology, 2004, 55(1):373.
[21] AFZAL F, KHURSHID R, ASHRAF M, et al.Chapter 13-reactive oxygen species and antioxidants in response to pathogens and wounding[J]. Oxidative Damage to Plants, 2014:397-424.
[22] CHEN Z, SILVA H, KLESSIG D F. Active oxygen species in the induction of plant systemic acquired resistance by salicylic acid[J]. Science(New York,N.Y.), 1993, 262(5141):1883.
[23] BRADLEY D J, KJELLBOM P, LAMB C J. Elicitor-and wound-induced oxidative cross-linking of a proline-rich plant cell wall protein:a novel, rapid defense response[J]. Cell, 1992, 70(1):21-30.
[24] ARUOMA O I, GROOTVELD M, BAHORUN T.Free radicals in biology and medicine:From inflammation to biotechnology[J]. Biofactors, 2010, 27(1/2/3/4):1-3.
[25] LIU J Z, HE C C SHEN F, et al. The crown plays an important role in maintaining quality of harvested pineapple[J]. Postharvest Biology&Technology, 2017, 124:18-24.
[26]陈艺晖,林河通,林艺芬,等.拟茎点霉侵染对龙眼果实采后果皮褐变和活性氧代谢的影响[J].中国农业科学, 2011, 44(23):4858-4866.
[27] SHARMA P, JHA A B, DUBEY R S, et al. Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions[J]. Journal of Botany, 2012,2012:1-26.
[28]杜秀敏,殷文璇,赵彦修,等.植物中活性氧的产生及清除机制[J].生物工程学报, 2001, 17(2):121-125.
[29] AGUDELO-ROMERO P, ERBAN A, REGO C, et al. Transcriptome and metabolome reprogramming in Vitis vinifera cv. Trincadeira berries upon infection with Botrytis cinerea[J]. Journal of Experimental Botany, 2015, 66(7):1769-1785.
[30]李晓云,王秀峰,吕乐福,等.外源NO对铜胁迫下番茄幼苗根系抗坏血酸-谷胱甘肽循环的影响[J].应用生态学报, 2013, 24(4):1023-1030.
[31] MANDAL S, MITRA A, MALLICK N. Biochemical characterization of oxidative burst during interaction between Solanum lycopersicum and Fusarium oxysporum f. sp. lycopersici[J]. Physiological&Molecular Plant Pathology, 2008, 72(1):56-61.
[32]刘晋,刘爱媛,陈维信.霜疫病菌侵染对荔枝果实生理变化的影响[J].西南园艺, 2006, 34(1):1-4.