石韦的抗病毒黄酮部位及有效化学成分
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摘要
目的:探究石韦的黄酮部位的抗病毒效果,并分析其有效成分.方法:采用标准病毒株或临床分离病毒株进行活性检测,通过观察病毒致细胞病变效应的发生率,计算黄酮部分抑毒指数TI,评价其抗病毒活性,并用HPLC-MS分析有效成分.结果:体积分数为30%的乙醇洗脱的石韦黄酮部位对肠道EV-71病毒抑制效果最好,用HPLC-MS分析体积分数为30%的乙醇洗脱石韦总黄酮部位的15个成分,其中3个酚酸类成分,12个黄酮类成分.结论:石韦黄酮部位有显著的抗病毒效果,可能是一种潜在的具有开发性的抗病毒药物.
Objective: To investigate the antiviral effect of the flavone fraction of pyrrosia lingua and to analyze its active components. Methods: The activity was detected by using the standard virus strains or the clinical isolates. By observing the incidence of the cytopathic effect induced by the viruses,the antiviral index TI was calculated and the antiviral activity was evaluated. The effective components were analyzed with HPLC-MS. Results: The fraction of 30% ethanol-eluted flavonoids had the best inhibitory effect on intestinal EV-71 virus. HPLC-MS analysis showed 15 components in the total flavone fraction of pyrrosia lingua eluted with 30% ethanol,including 3 phenolic acids and 12 flavonoids. Conclusion: The flavonoid fraction of pyrrosia lingua has significant antiviral effect and may be a potential antiviral drug.
Objective: To investigate the antiviral effect of the flavone fraction of pyrrosia lingua and to analyze its active components. Methods: The activity was detected by using the standard virus strains or the clinical isolates. By observing the incidence of the cytopathic effect induced by the viruses,the antiviral index TI was calculated and the antiviral activity was evaluated. The effective components were analyzed with HPLC-MS. Results: The fraction of 30% ethanol-eluted flavonoids had the best inhibitory effect on intestinal EV-71 virus. HPLC-MS analysis showed 15 components in the total flavone fraction of pyrrosia lingua eluted with 30% ethanol,including 3 phenolic acids and 12 flavonoids. Conclusion: The flavonoid fraction of pyrrosia lingua has significant antiviral effect and may be a potential antiviral drug.
引文
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[17]陈焱红.90例手足口病患儿的流行病学特征分析及危险因素研究[J].医疗装备,2016,29(4):59-60.CHEN Y H.Analysis of epidemiological characteristics and risk factors of 90 children with hand,foot and mouth disease[J].Medical Equipment,2016,29(4):59-60.
[2]刘绪林,刘文萍,王丽丽,等.石韦黄酮组分改善糖尿病肾病病理及减轻炎症反应作用研究[J].中国中药杂志,2018,43(11):2352-2357.LIU X L,LIU W P,WANG L L,et al.Study on the improvement of pathological changes and inflammatory response of diabetic nephropathy by flavonoids[J].China Journal of Chinese Materia Medica,2018,43(11):2352-2357.
[3]阎磊,邵凤民,朱清.复方石韦胶囊治疗脾肾气虚型糖尿病肾病临床研究[J].中药药理与临床,2017,33(6):149-151.YANG L,SHAO F M,ZHU Q.Clinical study on compound shiwei capsule in treating diabetic nephropathy of spleen and kidney qi deficiency[J].Pharmacology and Clinics of Chinese Medicine,2017,33(6):149-151.
[4]王亚茹,杨武德.黔产有柄石韦对大鼠利尿作用的初步研究[J].时珍国医国药,2017,28(11):2583-2585.WANG Y R,YANG W D.Preliminary study on the diuretic effect of scutellaria decoction in rats[J].Shizhen Guo Ma Guo Yao,2017,28(11):2583-2585.
[5]胡渊龙.石韦性味与功效演变考证[J].中医文献杂志,2017,35(3):13-14.HU Y L.Study on the evolution of Shi Wei's taste and efficacy[J].Journal of Traditional Chinese Medicine,2017,35(3):13-14.
[6]李晓宁,王向荣.复方石韦颗粒联合缬沙坦和前列地尔治疗慢性肾小球肾炎的临床研究[J].现代药物与临床,2016,31(10):1628-1631.LI X N,WANG X R.Clinical study of compound Shiwei granule combined with valsartan and alprostadil in the treatment of chronic glomerulonephritis[J].Drugs&Clinic,2016,31(10):1628-1631.
[7]WANG N,WANG J H,LI X,et al.Flavonoids from Pyrrosia petiolosa(Christ)Ching[J].J Asian Nat Prod Res,2006,8(8):753-756.
[8]郑民实.472种中草药抗单纯疱疹病毒的实验研究[J].中西医结合杂志,1990,10(1):39.ZHENG M S.Experimental study on 472 kinds of Chinese herbal medicine against herpes simplex virus[J].Journal of Chinese Integrative Medicine,1990,10(1):39.
[9]国家中医药管理局《中华本草》编委会.中华本草[M].上海:上海科技出版社,1999(2):253-258.EDITORIAL BOARD OF THE CHINESE MEDICINEADMINISTRATION OF THE STATE ADMINISTRATIONOF TRADITIONAL CHINESE MEDICINE.Zhonghua materia medica[M].Shanghai:Shanghai Science and Technology Press,1999(2):253-258.
[10]刘飞,徐佳馨,颜娓娓,等.中药佩兰体外抗病毒有效部位筛选[J].暨南大学学报(自然科学与医学版),2018,39(1):80-86.LIU F,XU J X,YAN W W,et al.Screening of effective antiviral parts of Chinese medicine Peilan in vitro[J].Journal of Jinan University(Natural Science&Medicine Edition),2018,39(1):80-86.
[11]牛文斐,王清,张加泽,等.中药白头翁体外抗病毒作用[J].暨南大学学报(自然科学与医学版),2016,37(4):345-349.NIU W F,WANG Q,ZHANG J Z,et al.Antiviral effect of Chinese medicine Pulsatilla chinensis in vitro[J].Journal of Jinan University(Natural Science&Medicine Edition),2016,37(4):345-349.
[12]颜娓娓,徐佳馨,王继锋,等.白头翁与蛴螬不同配伍比例体外抗病毒作用研究[J].长春中医药大学学报,2018,34(3):453-456.YAN W W,XU J X,WANG J F,et al.In vitro antiviral effect of different compatibility ratios of Pulsatillae and Poria[J].Journal of Changchun University of Traditional Chinese Medicine,2018,34(3):453-456.
[13]YAMASHITA H,MASUDA K,KOBAYASHI T,et al.Dammarane triterpe-noids from rhizomes of Pyrrosia lingua[J].Phytochemistry,1998,49(8):2461-2466.
[14]YAMASHITA H,MASUDA K,AGETA H,et al.Fern constituents:cyclo-hopenol and cyclohopanediol,noval skeletal triterpenoids from Rhizomes of Pyrrosia lingua[J].Chem Pharm Bull,1998,46(4):730-732.
[15]王楠,王金辉,程杰,等.有柄石韦化学成分的研究[J].沈阳药科大学学报,2003,20(6):425-427.WANG N,WANG J H,CHENG J,et al.Study on the chemical constituents of Shiwei Shiwei[J].Journal of Shenyang Pharmaceutical University,2003,20(6):425-427.
[16]李慧,贾新华.中药抗病毒机制的研究进展[J].江苏中医药,2015,47(6):82-85.LI H,JIA X H.Research progress on antiviral mechanism of traditional Chinese medicine[J].Jiangsu Journal of Traditional Chinese Medicine,2015,47(6):82-85.
[17]陈焱红.90例手足口病患儿的流行病学特征分析及危险因素研究[J].医疗装备,2016,29(4):59-60.CHEN Y H.Analysis of epidemiological characteristics and risk factors of 90 children with hand,foot and mouth disease[J].Medical Equipment,2016,29(4):59-60.